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1.
Foliar surfaces of nine pleurothallid orchids (Cryptophoranthus lepidotus L. O. Wms., Octomeria sp., Pleurothallis pidax Luer, P. poeppigii Lindl., P. revoluta (Ruiz & Pav.) Garay, Restrepiella ophiocephala (Lindl.) Garay & Dunsterv., Restrepia muscifera Rchb. f. ex Lindl., Scaphosepalum rapax Luer, Stelis endresii Rchb. f.), four nonpleurothallid orchids (Cyrtopodium punctatum (L.) Lindl., Encyclia cochleata (L.) Lemee, E. tampensis (Lindl.) Small, Paphiope-dilum hybrid) and five tillandsioid bromeliads (Catopsis nutans (Sw.) Griseb., Tillandsia fas-ciculata Sw., T. streptophylla Scheidw., T. stricta Soland., T. tectorum E. Morr.) were assayed for permeability to Ca, S and P ions. Compared to leaves of the atmospheric bromeliads, those of the orchids proved less permeable to Ca and S. Moisture exchange profiles were determined for two of the bromeliads and three orchids; bromeliad leaves were able to rehydrate completely, orchid leaves only partially. Absorptive capacities of trichomes borne by five taxa were tested by autoradiographic analysis. Unlike bromeliad trichomes, those of the orchids exhibited no capacity to accumulate 3H-leucine. These results and other considerations described here suggest that, unlike tillandsioid bromeliads, neither the pleurothallid nor the nonpleurothallid orchids examined in this survey rely heavily on shoots for mineral and moisture procurement. 相似文献
2.
FINN N. RASMUSSEN 《Botanical journal of the Linnean Society. Linnean Society of London》1985,91(4):447-456
RASMUSSEN, F. N., 1985. The gynostemium of Bulbophyllum ecornutum (J. J. Smith) J.J. Smith (Orchidaceae) . Stages in the development of the gynostemium of Bulbophyllum ecornutum demonstrate that the pollinium stalk is a hamulus in this and in a closely related species, B. gibbolabium . A hamulus arises by apical growth and reflexion of the median carpel. Hamuli have recently been discovered in several orchid genera, and a transverse fold of the rostellar apex is already known from a large group of orchids. The closely related B. cornutum has a quite different gynostemium structure. 相似文献
3.
Natural hybrids among the specialized terrestrial oil-secreting orchids of South Africa are extremely rare even where multiple closely related species co-occur. We found putative hybrids between Pterygodium catholicum Sw. and P. acutifolium Lindl., two closely related oil-secreting orchids that lack morphological barriers to inter-breeding. The purpose of this study was to confirm the parentage of the putative hybrids using molecular data from one nuclear (ITS) and two plastid (matK-trnK and trnL/F introns) DNA regions. Phylogenetic analyses of sequences as well as nucleotide substitution patterns of the putative hybrids, putative parents, and their closest relatives were consistent with a hypothesis of hybridization. We suggest that the hybrids were the result of visits to both orchids by a single species of oil-collecting bee during a brief period of flowering overlap. These results suggest that the rarity of hybrids between these orchids is due to factors other than genetic incompatibility. 相似文献
4.
Steven Dodsworth 《Curtis's Botanical Magazine》2016,33(4):294-302
Platystele misera (Lindl.) Garay is illustrated as the first of two species of Platystele Schltr., a genus of tiny Pleurothallid orchids from South and Central America. The ecology and distribution of Platystele are discussed, accompanied by brief notes on the cultivation of this species. 相似文献
5.
This work is the first to report the isolation and identification of bacteria colonizing the roots of tropical epiphytic orchids Acampe papillosa (Lindl.) Lindl. and Dendrobium moschatum (Buch.-Ham.) Swartz. and bacteria inhabiting inner layers of the aerial and substrate roots of A. papillosa. We showed by the example of this epiphyte that associative bacteria are present in large amounts on the aerial but not substrate roots. We isolated and identified bacteria from the substrate roots of D. moschatum and from its growth substrate (pine bark). The structure of the intercellular matrix of the associative bacteria was studied. 相似文献
6.
Water Relations of Tropical Epiphytes: III. EVIDENCE FOR CRASSULACEAN ACID METABOLISM 总被引:3,自引:0,他引:3
Five tropical epiphytes were examined for evidence of CrassulaceanAcid Metabolism (CAM), namely the orchids Eria velutina Lindl.,Dendrobium tortile Lindl. and Dendrobium crumenatum Sw., andthe ferns Pyrrosia adnascens (Forst.) Ching and Pyrrosia angustata(Sw.) Ching, family Polypodiaceae. Diurnal variations in leaftitratable acidity, diffusive conductance and water potentialwere measured at various levels of water stress. The three orchidsshowed typical CAM behaviour, namely large diurnal fluctuationsin leaf acidity, day-time closure and night opening of stomataand a very slow decline in water potential under stress. Theferns showed some evidence of CAM, but this was not as well-developedas had been reported for two other tropical epiphytic membersof the same family. Key words: Crassulacean acid metabolism, Tropical epiphytes, Water stress 相似文献
7.
东喜马拉雅地区是全球生物多样性的热点区域,西藏为其重要组成单元,明确该地区本底植物资源及地理分布对区域植物区系研究有着重要意义。该文报道了笔者在西藏考察中发现的西藏兰科(Orchidaceae)植物5个新记录属,即美柱兰属(Callostylis Blume)、异型兰属(Chiloschista Lindl.)、蛇舌兰属(Diploprora Hook. f.)、带叶兰属(Taeniophyllum Blume)和宽距兰属(Yoania Maxim.)。对应的5个新记录种,即美柱兰(Callostylis rigida Bl.)、异型兰(Chiloschista yunnanensis Schlechter)、蛇舌兰 [Diploprora championii(Lindl.)Hook. f.]、毛莛带叶兰(Taeniophyllum retrospiculatum King & Pantl.)和印度宽距兰(Yoania prainii King & Pantl.)。该文还附有新记录属、种的形态描述和特征图片。该研究结果进一步丰富了中国植物区系资料,拓宽了西藏兰科植物的记录,并对西藏及毗邻地区兰科植物多样性保护具有重要价值。 相似文献
8.
9.
以中国特有种沙芥[Pugionium cornutum(L.)Gaertn.]和濒危种斧形沙芥(Pugionium dolabratum Max-im.)的种子为研究材料,利用自然生境的沙土在室内进行种子萌发人工模拟试验,研究沙芥和斧形沙芥种子萌发、幼苗出土和幼苗生长对土壤含水量的适应性。结果表明:沙芥和斧形沙芥果实适宜萌发的土壤含水量范围均为6%~12%,适宜出苗的土壤含水量范围均为6%~16%。当土壤含水量低于2%时,果实不能萌发;当土壤含水量低于3%时,幼苗不能出土定居;当土壤含水量为6%~12%时,最有利于幼苗的生长。当土壤含水量分别为3%~8%和4%~8%时,沙芥和斧形沙芥植株根冠比最大。因此,沙芥和斧形沙芥种子萌发机制对沙生环境有着极强的适应性;植株将更多的同化物用于根系生长,以吸收更深层次的水分,从而适应含水量低的土壤。 相似文献
10.
This work is the first to report the isolation and identification of bacteria colonizing the roots of the tropical epiphytic orchids Acampe papillosa (Lindl.) Lindl. and Dendrobium moschatum (Buch.-Ham.) Swartz. and bacteria inhabiting inner layers of the aerial and substrate roots of A. papillosa. We showed by the example of this epiphyte that associative bacteria are present in large amounts on the aerial but not the substrate roots. We isolated and identified bacteria from the substrate roots of D. Moschatum and from its growth substrate (pine bark). The structure of the intercellular matrix of the associative bacteria was studied.Translated from Mikrobiologiya, Vol. 73, No. 6, 2004, pp. 825–831.Original Russian Text Copyright © 2004 by Tsavkelova, Cherdyntseva, Netrusov. 相似文献
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12.
Kiran Gogoi Suman Kumaria Pramod Tandon 《In vitro cellular & developmental biology. Plant》2013,49(3):248-254
A successful cryopreservation protocol for the long-term conservation of protocorms of two threatened and vulnerable orchids, Cymbidium eburneum Lindl. and Cymbidium hookerianum Rchb. f., was developed using encapsulation–dehydration. Protocorms were osmoprotected in liquid Murashige and Skoog medium (MS) containing 0.7 M sucrose for 20 h at 25?±?2°C on a rotary shaker, and incorporated into an encapsulation matrix [consisting of 3% (w/v) sodium alginate and 100 mM CaCl2]. The encapsulated protocorms, which were desiccated in a laminar airflow cabinet for 6 h, were able to withstand cryostorage in liquid nitrogen. Maximum regeneration into complete plantlets (72% for C. eburneum and 70% for C. hookerianum) of the cryostored, encapsulated protocorms was obtained using MS medium containing 3% sucrose and 0.8% agar. Using this protocol of cryopreservation, long-term preservation for ex situ conservation of these two threatened orchids can be accomplished. 相似文献
13.
The water relations of five species of tropical vascular epiphytesnative to Malaysia were studied. The species were ferns: Pyrrosiaadnascens (Forst.) Ching. and Pyrrosia angustata (Sw.) Ching.;orchids: Eria velutina Lindl., Dendrobium tortile Lindl. andDendrobium crumenatum Sw. Leaf resistance as a function of leafwater potential was measured for the two ferns. The criticalwater potential at which stomata closed was found to be highin each case; 0.75 MPa and 0.5 MPa respectively.The components of water potential were estimated with the pressurechamber as functions of relative water content. For each speciescell sap was found to be dilute, pressure potential low at fullturgor, and the change in relative water content between fullturgor and wilting point small. Small values of solute potentialat full turgor were also found for the ferns and E. velutinausing a vapour pressure osmometer. Values of the bulk modulusof elasticity of the leaf tissue for each species lay withinthe range of published data. The significance of these resultsfor the epiphytic way of life is discussed. Key words: Water potential, Epiphytes, Diffusive resistance, Orchid, Fern 相似文献
14.
Roots of representative epiphytic orchids were examined for anatomical detail, desiccation resistance and evidence of CAM activity. Those “shootless” taxa examined (Campylocentrum pachyrrhizum (Reichenb. f.) Rolfe, Harrisella porrecta Reichenb. f.) Fawc. & Rendle, and Polyradicion lindenii (Lindl.) Cogn. ex Urban) and a semi-shootless type (Kingidium taeniale (Lindl.) P. F. Hunt) bear thinner or eroded velamina and greater volumes of cortical intercellular space than do those of the leafy forms tested (Campylocentrum sellowii (Reichenb. f.) Rolfe, Encyclia tampensis (Lindl.) Small, Epidendrum radicans Pavon ex Lindl., Phalaenopsis amabilis (L.) Blume, Rangaeris amaniensis (Krzl.) Summerhayes and Vanda parishii (Reichenb. f.)). Shootless species also bear a more elaborate aeration apparatus at the velamen-cortex interface. Structurally distinct cortical cells located in this region may regulate gas exchange across the exodermis. Velamen thickness varies greatly among the ten species, as does the development of outer tangential walls of U cells in the underlying exodermis. Desiccation resistance under laboratory conditions was more closely related to root surface-to-volume ratio (S/V) than to any other measured anatomical parameter, including velamen development. Modes of carbon gain and the possible pathway for movement of fungus-borne carbon into an orchid's pool of assimilates are described, as is the possible significance of these processes to survival in forest canopy habitats. 相似文献
15.
报道了中国兰科植物一新记录种互生对叶兰[Neottia alternifolia(KingPantl.)Szlach.];并对紫婉石斛(Dendrobium transparens Wall.ex Lindl.)的形态特征、生境等进行了描述。紫婉石斛与兜唇石斛[Dendrobium aphyllum(Roxb.)C.E.Fischer]相似,区别在于唇瓣中央具深紫红色大斑块,唇瓣正面具柔毛;互生对叶兰是鸟巢兰属自养类型种类中唯一具有两片互生叶的种类,极易同该属其他种类区分。 相似文献
16.
Orchids are obligate mycoheterotrophic plants, relying on fungal nutrient resources to grow for their entire life or until they develop into photosynthetic seedlings. In Chile, orchids are represented by 7 genera and 63 species, 27 of which are endemic. Some Chilean species are considered endangered or rare, but many are insufficiently known. This study aims to isolate, culture, and identify fungal species found in symbiosis with the endemic Chilean orchids Chloraea collicensis Kraenzl. and Chloraea gavilu Lindl. for their potential to be used in future conservation programs. Roots of both species of orchids were collected in the field and those presenting pelotons were firstly cultured in agar-water and thereafter sub-cultured in potato dextrose agar media. Fungal colony growth was measured under the dissecting microscope. Fungal isolates from C. gavilu showed a higher growth rate than isolates from C. collicensis and could be used as inoculum for seed germination in further studies. Isolated colonies showed morphological characteristics of the form genus Rhizoctonia and presented two nuclei per cell. The ITS-nrDNA sequences confirmed their morphological identification as species of Tulasnella. 相似文献
17.
Microbial activity of trench leachates from shallow-land, low-level radioactive waste disposal sites. 总被引:1,自引:1,他引:0
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Trench leachate samples collected anoxically from shallow-land, low-level radioactive waste disposal sites were analyzed for total aerobic and anaerobic populations, sulfate reducers, denitrifiers, and methanogens. Among the several aerobic and anaerobic bacteria isolated, only Bacillus sp., Pseudomonas sp., Citrobacter sp., and Clostridium sp. were identified. Mixed bacterial cultures isolated from the trench leachates were able to grow anaerobically in trench leachates, which indicates that the radionuclides and organic chemicals present were not toxic to these bacteria. Changes in concentrations of several of the organic constituents of the waste leachate samples were observed due to anaerobic microbial activity. Growth of a mixed culture of trench-water bacteria in media containing a mixture of radionuclides, 60Co, 85Sr, and 134,137Cs, was not affected at total activity concentrations of 2.6 X 10(2) and 2.7 X 10(3) pCi/ml. 相似文献
18.
Liebel HT Bidartondo MI Preiss K Segreto R Stöckel M Rodda M Gebauer G 《American journal of botany》2010,97(6):903-912
We compared the nutritional modes and habitats of orchids (e.g., autotrophic, partially or fully mycoheterotrophic) of the Mediterranean region and adjacent islands of Macaronesia. We hypothesized that ecological factors (e.g., relative light availability, surrounding vegetation) determine the nutritional modes of orchids and thus impose restrictions upon orchid distribution. Covering habitats from dark forests to open sites, orchid samples of 35 species from 14 genera were collected from 20 locations in the Mediterranean and Macaronesia to test for mycoheterotrophy. Mycorrhizal fungi were identified via molecular analyses, and stable isotope analyses were applied to test whether organic nutrients are gained from the fungal associates. Our results show that orchids with partial or full mycoheterotrophy among the investigated species are found exclusively in Neottieae thriving in light-limited forests. Neottioid orchids are missing in Macaronesia, possibly because mycoheterotrophy is constrained by the lack of suitable ectomycorrhizal fungi. Furthermore, most adult orchids of open habitats in the Mediterranean and Macaronesia show weak or no N gains from fungi and no C gain through mycoheterotrophy. Instead isotope signatures of some of these species indicate net plant-to-fungus C transfer. 相似文献
19.
Background and Aims A significant number of species assigned to the Neotropical orchid sub-tribe Oncidiinae reward insect pollinators with oil produced in floral glands termed elaiophores. The latter may be glabrous (epithelial elaiophores) or hirsute (trichomal elaiophores). Although the detailed anatomy and ultrastructure of epithelial elaiophores have been studied for a number of genera, such as Oncidium Sw., Gomesa R. Br. and Trichocentrum Poepp. & Endl., hitherto, trichomal elaiophores have been investigated only for a single species of Oncidiinae, Ornithocephalus ciliatus Lindl. Furthermore, this is the only representative of the Ornithocephalus clade to be investigated to date. Here, an examination is made of the elaiophore anatomy and ultrastructure of a further four species currently assigned to this clade (Ornithocephalus gladiatus Hook., Phymatidium falcifolium Lindl., Zygostates grandiflora (Lindl.) Mansf. and Zygostates lunata Lindl.) and the results compared with those obtained for other Oncidiinae. Methods Elaiophore structure was examined for all species at three stages of flower development: closed bud, first day of anthesis and final stage of anthesis, using light microscopy, fluorescence microscopy, scanning electron microscopy, transmission electron microscopy and histochemistry. Key Results Elaiophores of O. gladiatus occur upon the lateral lobes of the labellum and display characters intermediate between those of typical epithelial and trichomal elaiophores, in that they are largely glabrous, consisting mainly of cuboidal epidermal cells, but bear short, unicellular hairs proximally. By contrast, the elaiophores of all the other species investigated occur on the callus and are of the trichomal type. In P. falcifolium, these unicellular hairs are capitate. In all species, oil secretion commenced at the closed floral bud stage. Ultrastructurally, the mainly trichomal elaiophores of the four representatives of the Ornithocephalus clade closely resembled the epithelial elaiophores of other Oncidiinae, in that their cells displayed an organelle complement typical of lipid-secreting cells. However, in some taxa, a number of noteworthy characters were present. For example, the elaiophore cuticle of O. gladiatus and P. falcifolium was bi-layered, the outer layer being lamellate, the inner reticulate. The cuticle of Z. grandiflora and Z. lunata was also lamellate, but here, a reticulate layer was absent. Accumulation of secreted oil resulted in the localized distension of the cuticle. Cuticular cracks and pores, however, were absent from all species. The walls of the secretory cells of Z. grandiflora were also atypical in that they had short protuberances or ingrowths, and contained cavities which are thought to be involved in the secretory process. Conclusions Of the species investigated, most displayed similar anatomical organization, their trichomal elaiophores occurring on the labellar callus. They, thus, differ from many other members of the Oncidiinae, where epithelial elaiophores are found either on the callus, or on the lateral lobes of the labellum. However, ultrastructurally, all elaiophores, whether those of representatives of the Ornithocephalus clade, or those of other oil-secreting Oncidiinae, possessed a similar complement of organelles, regardless of whether the elaiophores were trichomal or epithelial. In view of the latter, and the similar chemical composition of oils derived from all Oncidiinae investigated to date, it is probable that position and type of elaiophore, and possibly the structure of the overlying cuticle, play an important role in pollinator selection in these oil-secreting orchids. 相似文献
20.
广西壮族自治区位于我国南部,气候湿润,生境复杂,具有丰富的物种多样性。该文报道广西兰科植物13个新记录种,即旗唇兰[Kuhlhasseltia yakushimensis(Yamamoto) Ormerod]、紫茎兰(Risleya atropurpurea King&Pantl.)、指柱兰(Stigmatodactylus sikokianus Maxim. ex Makino)、二尾兰(Vrydagzynea nuda Bl.)、深圳拟兰(Apostasia shenzhenica Z. J. Liu&L. J. Chen)、拟泰国卷瓣兰(Bulbophyllum nipondhii Seidenf.)、南岭叠鞘兰(Chamaegastrodia nanlingensis H. Z. Tian&F. W. Xing)、垂叶斑叶兰(Goodyera pendula Maxim.)、四腺翻唇兰(Hetaeria anomala Lindl.)、褐花羊耳蒜(Liparis brunnea Ormerod)、聚叶钗子股(Luisia appressifolia Av... 相似文献