新疆八个民族指纹崎线离解的调查

本文首次报道我国新疆8个民族的中小学生8000人（男女各4000人）的指纹蜻线离解出现频率。结果表明,十指指腹有肤纹覆盖的任何区域均可出现蜻线离解,其频剖男高于女。民族间频率高低亦有一定差异．     

宁夏回、汉族指纹白线的研究

分析了宁夏回族 390 例 (男 :189 例 ,女 :201 例) 、汉族 425 (男 :217 例 ,女 208 例) 指纹白线的分布特 征。结果表明 : 宁夏回族指纹白线出现率为 : 19103 % (男 : 17109 % ,女 : 20185 %) ; 汉族为 : 17155 % (男 : 16122 % ,女 :18194 %) 。同一民族指纹白线出现率女性高于男性 ,有显著性差异 (回 :χ2 = 81916 ,P < 0101 ; 汉 :χ2 = 51434 ,P < 0105) ;回、汉族间指纹白线出现率无显著性差异(χ2 = 21956 ,P > 0105) 。     

糖尿病患者指纹白线的分析

作者对298例糖尿病患者的指纹白线的出现率进行了调查,结果表明,糖尿病患者的指纹白线出现率明显高于同一地区正常人群,差异高度显著(P<0 .01)。其中女性指纹白线的出现率明显高于男性,差异显著(P<0.05),较某些遗传病指纹白线的出现率低。     

民族药用植物指纹数据库的构建研究

通过对四川省时采集的民族药用植物标本进行基因和化学指纹图谱、药用价值、民族传统利用等信息的整理,用SQL Server 2000建立一个民族药用植物指纹的开放式关系数据库初步框架。该数据库重点体现药用植物的民族利用特色,同时包括各种指纹图谱图片。该数据库不仅可以快速、方便地检索和查询有关民族药用植物的背景资料、药用价值以及指纹图谱信息,而且可为四川省内民族药用植物资源的保护和利用提供基础。     

中国十一个少数民族的皮纹研究 Ⅰ.指纹

研究了中国11个少数民族(12个群体)5013人的指纹花样和指纹脊线数,计算出各项基本参数,比较分析了不同性别、左右侧、不同民族和人种间的差异以及指纹花样和指纹脊线数在不同手指上的分布特点。分析表明,这些民族的指纹具有各自的特点又具有蒙古人种的一般特性。     

新疆伊犁六个民族的初潮年龄

1983年7月至1984年7月,用回忆法调查了新疆伊犁地区3972名12—23岁的女性的初潮年龄,其中哈萨克族924人,维吾尔族864人,锡伯族486人,回族209人,蒙古族216人,汉族1273人。其平均初潮年龄是:哈萨克族14.37±1.09岁,维吾尔族14.34±1.41岁,蒙古族14.21±1.05岁,回族13.89±1.32岁,锡伯族13.78±1.02岁,汉族13.61±1.11岁。各族中均以七、八、九三个月来月经的最多。此外,还发现六个民族中1969—1972年出生的与1961—1964年出生的相比,平均初潮年龄都明显提前。在八年中,回族提前2.67岁,蒙古族提前1.90岁,锡伯族提前1.78岁,汉族提前1.64岁,哈萨克族提前1.17岁,维吾尔族提前1.13岁。     

5个生姜品种的蛋白电泳指纹图谱研究

目的：研究5个生姜品种的鉴定。方法：利用蛋白质聚丙烯酰胺凝胶电泳（PAGE）指纹图谱分析、鉴别5个生姜品种。结果：5个样品的蛋白质PAGE指纹图谱具有一定差异。结论：PAGE指纹图谱可用于生姜品种鉴定与质量评价。     

奶牛和肉牛6个STR基因座遗传多态性研究The Polymorphism Distributions of Six STR Loci in Dairy Cattle and Beef Cattle

利用PCR技术和复合电泳银染技术检测奶牛和肉牛BM2113、BM1862、BMc701、BM2934、TGLA122、BM720等6个STR基因座的多态性分布，并计算该6个基因座的基因频率（Pi）、个体鉴别力（DP）、杂合度（H）、多态信息含量（PIC）、和非父排除概率（PE）。结果显示：6个STR基因座的基因型分布符合Hardy-Weinberg平衡，奶牛中6个STR基因座中BM2113 基因座的DP、H和PIC最高，TGLA122基因座的PE最高。 6个STR基因座的累积个体鉴别力 （CDP）为 0.99997 ，累积非父排除能力（CPE）为 0.98827 。肉牛中6个STR基因座中BM1862 基因座的DP、H、PIC、PE都是最高，6个STR基因座的累积个体鉴别力（CDP）为 0.99999，累积非父排除能力（CPE）为 0.99578 。结果表明，6个STR基因座可用于牛的遗传连锁分析、个体识别和亲子鉴定等研究领域。Abstract：The polymorphism distributions of six STR loci，BM2113，BM1862，BMc701，BM2934，TGLA122，and BM720 were detected in cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver staining. Gene frequency (Pi)，power of discrimination (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (PE) were calculated．All loci obey Hardy-Weinberg equilibrium. DP, H and PIC of BM2113 locus, and PE of TGLA122 locus are the biggest among six STR loci in Holstein Friesian. Cumulate DP of six STR loci is 0.99997, Cumulate PE of six STR loci is 0.98827. DP, H, PIC and PE of BM1862 loci are the biggest among six STR loci in beef. Cumulate DP of six STR loci is 0.99999, Cumulate PE of six STR loci is 0.99578. These results showed that the six STR loci could be used as linkage analysis, individual identification and paternity test in cattle.     

新疆维、哈两民族人群血浆中谷氨酰胺的含量及其与2型糖尿病关系的研究

目的 探讨谷氨酰胺与2型糖尿病的关系.方法 采用高效液相色谱仪(HPLC)来测定新疆维、哈两民族血浆中谷氨酰胺的含量;使用放免法测定空腹血清胰岛素(FINS)含量;采用全自动生化分析仪来测定空腹血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDLC)、低密度脂蛋白(LDLC)的含量.结果 维吾尔族正常人与糖尿病患者之间血浆中谷氨酰胺含量差异有统计学意义(P＜0.01);哈萨克族正常人与糖尿病患者之间血浆中谷氨酰胺含量差异无统计学意义;维哈两民族的正常人血浆中谷氨酰胺含量差异有统计学意义(P＜0.01);维哈两民族的糖尿病患者血浆中谷氨酰胺的含量差异有统计学意义(P＜0.05).维哈两民族人群血浆中的谷氨酰胺含量与空腹血糖(FBG)水平呈负相关(r=-0.4858,P＜0.001),与FINS水平呈正相关(r =0.236,P＜0.05),与胰岛素分泌指数(HOMA-IS)呈正相关(r=0.52,P=0.001),与TG呈负相关(r=-0.4330,P＜0.01),与LDLC呈负相关(r=-0.4077,P＜0.01),与HDLC呈正相关(r=0.2553,P＜0.01).结论 谷氨酰胺可能是2型糖尿病的一个保护性的影响因素.     

新疆维吾尔族HLA-Cw基因座遗传多态性调查 The Study of HLA-Cw Polymorphism in Uygur Population

采用序列特异性寡核苷酸探针杂交技术（PCR-SSOP）对146位新疆维吾尔族无关个体HLA-Cw基因座进行基因分型,研究该民族HLA-Cw基因座遗传多态性,建立新疆维吾尔族HLA-Cw基因频率数据库。检出18种等位基因,基因频率分布在0.0069~02460,其中HLA-Cw*04、07、08、14基因频率比较高,基因频率分别为02460、0.1151、0.1010、0.1202,共占新疆维吾尔族可检出等位基因的58.23%,PCR-SSOP分型技术使新疆维吾尔族HLA-Cw基因座空白基因频率降至0.0064。经χ2检验,基因型分布符合Hardy-Weinberg平衡定律。建立民族HLA-Cw基因座基因频率数据库,为临床器官移植配型、人类学、法医学提供重要的群体遗传学资料。 Abstract:The HLA-Cw loci polymorphism in Uygur population was investigated using the PCR- sequence specific oligonucleotide probe (SSOP) method,and the genetic database on the distribution of gene frequency of the HLA-Cw loci was established.From 146 individuals of Uygur population,18 HLA-Cw alleles were detected.The gene frequency was from 0.0069 to 0.2460.The four most common alleles were HLA-Cw*04(24.60%)、07(11.51%)、08(1010%)、14(12.02%),and they covered 58.23% of total alleles detected from Uygur population.We have made a survey of HLA-Cw alleles frequencies in a Uygur population,with blank frequency being lowered to 0.0064.The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by hi-square test.The frequency data can be used in forensic and paternity tests to estimate the frequency of a DNA profile in the Uygur population,transplant matching and anthropology.     

辽宁满族和汉族指纹白线正常值分析

本文研究了1060例 (满族：460,汉族：600;男女各半) 满族和汉族指纹白线。满族和汉族指纹白线出现率分别是7.11％和31.73％。结果表明民族间的指纹白线有他们各自的特征并做了性别和手别间差异的比较分析。     

汉、回、蒙古族拇指类型、环食指长、扣手、交叉臂及惯用手的研究

作者于1996年在内蒙古调查了汉、回、蒙古族5项人类遗传学经典指标(拇指类型、环食指长、扣手、交叉臂、惯用手)。研究结果显示:(1)3个民族间拇指类型、扣手出现率存在显著性差异,交叉臂、惯用手出现率则无显著性差异,环食指长出现率蒙-汉、蒙-回间存在显著性差异;(2)拇指类型、扣手、惯用手出现率无性别间差异,环食指长出现率男女间存在显著性差异;(3)惯用手与扣手、惯用手与交叉臂间存在明显的相互关系,交叉臂与扣手之间则无关;(4)与国外人群比较,3个民族环指长出现率高,交叉臂R型出现率较高,扣手R型出现率较低,惯用手L型出现率高于印度的一些群体。 Abstract:Authors in vestigated 5 general indexes of anthrotogical genetics including pollical type,palmar digital formula,hand clasping,arm folding and handedness in Han,Hui and Mongol nationalities in 1996.The results showed as follows:(1)There were significant differences in the frequency of pollical type and hand clasping in 3 nationalities,but those of arm folding and handedness showed nosignificant difference and the frequencies of palmar digital formula between the Mongol and the Hui revealed significant difference.(2)There were no significant sexual difference in the frequency of pollical type,hand clasping and handedness while the long type (R) of ring finger revealed significant sexual difference.(3)There were obvious correlations between handedness and hand clasping,handedness and arm folding but no relation between arm folding and hand clasping.(4)In comparison with foreign ethnic groups,the 3 nationalities showed higher frequencies of long type (R) of ring finger and right-arm folding but the frequence right-hand clasping revealed slightly lower.The findings showed higher frequence of Left-Handedness than that of Indian population.     

果蝇心脏发育标记基因Hand多克隆抗体的制备

制备果蝇心脏标记基因Hand抗体对研究果蝇心脏发育具有重要意义。从果蝇体内提取出总RNA,反转录得到果蝇的cDNA,将其作为模板PCR得到Hand基因部分片段,将片段连接到pET-28a上,构建重组质粒pET一28a—Hand,将重组质粒转化rosetta受体菌,IPTG诱导表达,表达产物经镍柱纯化,SDS—PAGE电泳分析,结果表明Hand基因在大肠杆菌中成功表达,表达的Hand融合蛋白分子量大约为24kD,经镍柱纯化后获得了高纯度可溶性的Hand蛋白。     

内蒙地区蒙古,汉,回,朝鲜族指纹白线分析

本文分析了内蒙地区３５９７例蒙古、汉、回、朝鲜族人指纹白线分布特征,并探讨了指纹白线与皱纹掌和某些掌褶纹类型的关系。结果表明４个民族的指纹白线出现率：蒙古族１２．５７％、汉族１５．１７％、回族１８．８０％、朝鲜族１４．７８％。各民族的指纹白线出现率具有性别间和手别间明显差异,亦具民族间显著差异。此外,在这４个民族中,具指纹白线者的皱纹掌和桥贯型、悉尼型掌褶纹的相对出现率均较高。     

四川彝族成人手长、手宽和指长推断身高及性别判别的研究

本文通过对460例(男354, 女106) 20—50岁中国四川凉山彝族健康成年人右利手手长、手宽、五指长度及确切身高的测量, 研究了四川彝族成人手长、手宽、指长推断身高和性别判别的方法。建立了推断身高的22个回归方程和2个性别判别函数, 其中手长推断身高和手长、手宽判别性别的效果较好。     

Hand foot and mouth disease due to enterovirus 71 in Malaysia

Hand foot and mouth disease is a febrile sickness complex characterized by cutaneous eruption (exanthem) on the palms and soles with simultaneous occurrence of muco-cutanous vesiculo-ulcerative lesions (enanthem) affecting the mouth.The illness is caused by a number of enteroviruses with coxsackievirus A16 and enterovirus 71 as the main causative agents.Human enterovirus 71 (EV71) belongs to the species Human enterovirus A under the genus Enterovirus within the family Picornaviridae.EV71 has been associated with an array of clinical diseases including hand foot and mouth disease (HFMD),aseptic meningitis,encephalitis and poliomyelitis-like acute flaccid paralysis.A large outbreak of HFMD due to highly neurovirulent EV71 emerged in Malaysia in 1997,and caused 41deaths amongst young children.In late 2000,a recurrence of an outbreak of HFMD occurred in Malaysia with S fatalities in peninsular Malaysia.Outbreak of HFMD due to EV71 recurred in 2003 with an unknown number of cases and mortalities.A similar outbreak of HFMD with 2 recorded deaths in young children occurred in peninsular Malaysia in late 2005 and this was followed by a larger outbreak in Sarawak (Malaysian Borneo) with 6 reported fatalities in the early part of 2006.The current on-going outbreak of HFMD started in peninsular Malaysia in epidemiological week 12 of 2010.As with other HFMD outbreaks in Malaysia,both EV71 and CA16 were the main aetiological viruses isolated.In similarity with the HFMD outbreak in 2005,the isolation of CA16 preceded the appearance of EV71.Based on the VP 1 gene nucleotide sequences,4 sub-genogroups of EV71 (C1,C2,B3 and B4) co-circulated and caused the outbreak of hand,foot and mouth disease in peninsular Malaysia in 1997.Two sub-genogroups (C1 and B4) were noted to cause the outbreak in 2000 in both peninsular Malaysia and Sarawak.EV71 of sub-genogroup B5 with smaller contribution from sub-genogroup C1 caused the outbreak in 2003.In the 2005 outbreak,besides the EV71 strains of sub-genogroup C1,EV71 strains belonging to sub-genogroup B5 were isolated but formed a cluster which was distinct from the EV71 strains from the sub-genogroup B5 isolated in 2003.The four EV71 strains isolated from clinical specimens of patients with hand,foot and mouth disease in the Sarawak outbreak in early 2006 also belonged to sub-genogroup B5.Phylogenetic analysis of the VP1 gene suggests that the EV71 strains causing the outbreak in Sarawak could have originated from peninsular Malaysia.Epidemiological and molecular data since 1997 show the recurrence of HFMD due to EV71 in Malaysia every 2 to 4 years.In each of the past outbreaks,more than one sub-genogroup of the virus co-circulate.