Photoinduction of circular F-actin on chloroplast in a fern protonemal cell

Summary Circular F-actin on a photooriented chloroplast was observed by rhodamine-phalloidin staining in the fernAdiantum protonemal cells in which phytochrome- or blue light receptor-mediated intracellular photoorientation of chloroplasts was induced. The circular structure located along the edge of chloroplast on the side facing the plasma membrane but not on the opposite side. Most of the chloroplasts in protonemal cell have dumbbell-shape and the circular ring-like structure was found on each half of the dumbbell. The structure was not observed in the cells which were kept in the dark, indicating the change of F-actin organization by the light condition. Possible role of the structure on the anchorage of chloroplast in its intracellular photoorientation was discussed.     

Photoorientation of chloroplasts in protonemal cells of the fernAdiantum as analyzed by use of a video-tracking system

Photoorientation of chloroplasts mediated by phytochrome and blue light-absorbing pigment in protonemal cells of the fernAdiantum was studied by use of inhibitors of the cytoskeleton and was analyzed with a video-tracking system. The photoorientation responses were inhibited by cytochalasin B and by N-ethylmaleimide (NEM) but not by colchicine, suggesting that the photomovement depends on the actomyosin system. In the dark, chloroplasts moved randomly, being independent of one another. After induction of photoorientation by polarized red light, most chloroplasts that had been located at the margin of cells moved almost perpendicularly to the cell axis toward the site of photoorientation. This type of movement was hardly ever observed in the dark. Under polarized blue light, such specific movements were less evident but were still observed in the case of a few chloroplasts. After photoorientation was complete, chloroplasts still moved in random directions but their mobility was lower than that in the dark, indicating the presence of some anchoring mechanism. When EGTA was applied, photoorientation was inhibited but this inhibition was overcome by the addition of CaCl₂. Video-tracking of chloroplasts in the dark revealed that the mobility of chloroplasts was higher in medium with EGTA than in medium with EGTA plus CaCl₂ and that many of the chloroplasts moved jerkily in the medium with EGTA. This change in the nature of movements was also seen under polarized light, resulting in the disturbance of photoorientation. These results indicate that the inhibition of photoorientation at low concentrations of Ca²⁺ ions may be due to change in the nature of chloroplast movement.     

Photoregulation systems for light-oriented chloroplast movement

Movements of the chloroplasts induced by a directional stimulus of light are found to occur in various plant materials ranging from algae to terrestrial angiosperms. Depending on the fluence rate of light, chloroplasts move toward the area of the maximum light absorption and escape from it, which are named as low- and high-fluence-rate responses, respectively. In most materials the effective wavelengths are exclusively found in the blue-UV region of spectrum, (a) flavin pigment(s) being considered as the photoreceptor, while in a few species of plants phytochrome is involved. The arrangement of chloroplasts as a result of the movement depends on the orientation of the electrical vector of light, which reflects the dichroic orientation of the photoreceptor for perception of light direction. Photosystems involved in these responses, however, are not only for perception of light direction, but also for realization of the movement and for holding of the chloroplasts in the reached site. Possible interactions and dual roles of photosystems in regulation of chloroplast movement are discussed.     

Mapping the interaction sites of <Emphasis Type="Italic">Aspergillus nidulans</Emphasis> phytochrome FphA with the global regulator VeA and the White Collar protein LreB

Aspergillus nidulans senses red and blue-light and employs a phytochrome and a Neurospora crassa White Collar (WC) homologous system for light perception and transmits this information into developmental decisions. Under light conditions it undergoes asexual development and in the dark it develops sexually. The phytochrome FphA consists of a light sensory domain and a signal output domain, consisting of a histidine kinase and a response regulator domain. Previously it was shown that the phytochrome FphA directly interacts with the WC-2 homologue, LreB and another regulator, VeA. In this paper we mapped the interaction of FphA with LreB to the histidine kinase and the response regulator domain at the C-terminus in vivo using the bimolecular fluorescence complementation assay and in vitro by co-immunoprecipitation. In comparison, VeA interacted with FphA only at the histidine kinase domain. We present evidence that VeA occurs as a phosphorylated and a non-phosphorylated form in the cell. The phosphorylation status of the protein was independent of the light receptors FphA, LreB and the WC-1 homologue LreA.     

The Protonema of Chara fragilis Desv.: Regenerative Formation,Photomorphogenesis, and Gravitropism

When exposed to constant white light for four weeks, isolated nodes of Chara fragilis Desv. regenerated side branches, rhizoids, and multicellular protonemata, the latter being similar to those germinated from oospores. When kept in darkness the nodes developed protonemata exclusively. These were single-celled, colourless, and tip-growing and, with the light microscope, they looked like rhizoids. Upon exposure to blue light, but not to red or far-red, the growth rates of the protonemata rapidly declined, the cell apices swelled, and the nucleus migrated acropetally. Within 24 h the cells went through the first of a series of divisions resulting in the formation of multicellular protonemata. When returned to darkness after a blue light pulse of 5 h the cell divisions proceeded normally, but the protonemata showed etiolated growth. While growth of the internode was drastically promoted, the development of the multicellular apex and the lateral initial were suppressed. Both uni- and multicellular etiolating protonemata showed negative gravitropism but were phototropically insensitive. It is argued that the single-celled protonema is an organ specialized for the penetration of mud covering the nodes or oospores of Chara and thus serves to search for light, comparable to etiolated hypocotyls and stems in seedlings of higher plants.     

Reorganization of microfilaments in protonemal tip cells of the mossCeratodon purpureus during the phototropic response

Summary The F-actin distribution in caulonemal tip cells of the mossCeratodon purpureus was examined by rhodamine-phalloidin staining. Gravitropically-growing caulonemal tip cells of the moss possess a distinct alignment of microfilaments (MFs) in their apices. Axially oriented actin bundles run from subapical regions to the apex where they converge towards a central area of the tip, although bundles are absent from the central area itself thus forming a collar-like structure. During a unilateral red light irradiation the actin strands of the apical dome become reoriented towards the irradiated apical flank and still surround an area free of MFs, the point of prospective outgrowth. This process is closely correlated with the morphological effect of bulging and precedes the light-directed outgrowth. The collar structure is essential for the tubular growth form. In darkness, under the influence of antimicrotubule agents the structure is decomposed, the actin strands drift along the cell flanks and finally accumulate in randomly distributed areas where further growth takes place. The microtubules (MTs) are not involved in the phytochromemediated reorientation of the microfilaments. Unilateral red light suppresses the distorting effect of antimicrotubule drugs and restores the collar structure with a pronounced light-directed orientation. Instead, the MTs seem to be responsible for restricting the reorientation to the cell tip. This notion is based on the observation that the small area in the apical dome, which is normally the exclusive location of the light-regulated MF rearrangement, extends towards the cell base when MT inhibitors are applied before the unilateral red light irradiation. This in turn leads to a non-tubular expansion of the light-directed cell flank.Abbreviations DIG differential interference contrast - DMSO dimethyl sulfoxide - EGTA ethyleneglycol-bis-(beta-aminoethylether) N,N,N,N-tetraacetic acid - MF microfilament - MT microtubule - MTSB microtubule stabilizing buffer - MBS 3-maleimidobenzoic     

Structural basis for the red light induced repolarization of tip growth in caulonema cells ofCeratodon purpureus

Summary Two dynamic changes are associated with the phytochrome-regulated phototropic response in tip cells of the mossCeratodon purpureus: a tip-located gradient shift of chlortetracy-cline (CTC)-stained calcium and a structural reorganization of apical microfilaments (MFs). We examined the interdependence of these processes. Cells were treated with the antimicrotubule drug oryzalin, the antimicrofilament drug cytochalasin-D, and the calcium channel blocker nifedipine. respectively. The effects on phototropic growth, on the structural alignment of the cytoskeleton (microtubules, MTs; microfilaments) and on the distribution of CTC-stained calcium were studied under each of these conditions. In gravitropically growing tip cells the apical MFs form a cortical collar-like structure, consisting of actin bundles with a parallel axial alignment. These MFs point towards the presumptive growing point, a weakly stained region in the tip of the cell from which bundles are absent. MTs are present in the cortex and in the endoplasm of the tip, predominantly oriented longitudinally. The MTs converge within the central apex. The cells show a steep tip-to-base CTC-calcium gradient with its highest signal in the central apex. Destruction of MTs by 1 M oryzalin induces several translocational effects: (i) the growing zone and phototropic outgrowth shift from the apex to subapical parts of the cell; (ii) the structural integrity of the apical MFs and the tip-to-base alignment of the CTC-calcium gradient are disturbed; and (iii) the red light induced gradient shift and the reorientation of MFs proceed in an expanded area spanning from the tip to subapical parts of the cell. Cytochalasin-D (10 g/ml) destroys the MFs. Under these conditions tip growth stops and the phototropic outgrowth is suppressed. The apical MT-structure and the CTC-calcium gradient are not influenced by the agent. Unilateral red light still induces the light-directed translocation of the gradient. Tip cells memorize a unilateral irradiation applied during growth inhibition with cytochalasin-D. After recovery in darkness the cells start to grow in the former light direction. The restoration of the MFs precedes the outgrowth. The structural alignment of the rebuilt actin bundles indicates the future growth direction. The calcium channel blocker nifedipine (10 M) also inhibits tip growth and concurrently phototropic outgrowth. Nifedipine destroys the CTC-calcium gradient and apical MFs; MTs are not influenced by the channel blocker.Abbreviations CTC chlortetracycline - DIC differential interference contrast - DMSO dimethyl sulfoxide - EGTA ethyleneglycol-bis-(-aminoethylether) N,N,N-N-tetraacetic acid - MBS 3-maleimidobenzoic acid N-hydroxysuccimide ester - MF microfilament - MT microtubule - MTSB microtubule stabilizing buffer - PIPES piperazine-N,N-bis(2-ethane-sulfonic acid) - RP rhodamine labeled phalloidin     

Preprophase Band in Fern Protonemata: Mechanism of Development and Comparison with Flowering Plants

Adiantum capillus-veneris L. This review focuses on mechanisms of preprophase band development in fern protonemata. Received 12 December 1999/ Accepted in revised form 18 January 2000     

Blue- and red-light action in photoorientation of chloroplasts in Adiantum protonemata

An action spectrum for the low-fluencerate response of chloroplast movement in protonemata of the fern Adiantum capillus-veneris L. was determined using polarized light vibrating perpendicularly to the protonema axis. The spectrum had several peaks in the blue region around 450 nm and one in the red region at 680 nm, the blue peaks being higher than the red one. The red-light action was suppressed by nonpolarized far-red light given simultaneously or alternately, whereas the bluelight action was not. Chloroplast movement was also induced by a local irradiation with a narrow beam of monochromatic light. A beam of blue light at low energy fluence rates (7.3·10^-3-1.0 W m^-2) caused movement of the chloroplasts to the beam area (positive response), while one at high fluence rates (10 W m^-2 and higher) caused movement to outside of the beam area (negative response). A red beam caused a positive response at fluence rates up to 100 W m^-2, but a negative response at very high fluence rates (230 and 470 W m^-2). When a far-red beam was combined with total background irradiation with red light at fluence rates causing a low-fluence-rate response in whole cells, chloroplasts moved out of the beam area. When blue light was used as background irradiation, however, a narrow far-red beam had no effect on chloroplast distribution. These results indicate that the light-oriented movement of Adiantum chloroplasts is caused by red and blue light, mediated by phytochrome and another, unidentified photoreceptor(s), respectively. This movement depends on a local gradient of the far-red-absorbing form of phytochrome or of a photoexcited blue-light photoreceptor, and it includes positive and negative responses for both red and blue light.Abbreviations BL blue light - FR far-red light - Pfr far-red-absorbing form of phytochrome - Pr red-absorbing form of phytochrome - R red light - UV ultraviolet     

Reorganization of the cortical cytoskeleton in tip-growing fern protonemal cells during phytochrome-mediated phototropism and blue light-induced apical swelling

Summary Circular arrays of cortical microtubules (MTs) and microfilaments (MFs) are found in the subapical region of tip-growing protonemal cells of the fernAdiantum capillus-veneris. Reorganization of the two cytoskeletal structures during phytochrome-mediated phototropism and blue light-induced apical swelling was investigated by double-staining of MTs and MFs with rhodaminephalloidin and an indirect immunofluorescence method with tubulinspecific antibody. Before any growth responses were detectable, the MF and MT structures were reorganized according to similar patterns in both photoresponses, that is, oblique orientation and transient disappearance of the structures occurred during the phototropic response, and the disappearance of the structures occurred during apical swelling. The reorganization of MF structures clearly preceded that of the MT structures in the phototropic response. In the case of apical swelling, both types of circular array disappeared with an almost identical time course.These results provide evidence for the significant role of the circular organization of MFs as well as of MTs, in the light-induced growth responses of tip-growing fern protonemal cells. Possible roles of the circular array of MFs in the regulation of tip growth are discussed.Abbreviations DMSO dimethylsulfoxide - PIPES piperazine-N,N-bis(2-ethane-sulfonic acid) - EGTA ethyleneglycol-bis-(-aminoethylether)-N,N,N,N-tetraacetic acid - PMSF phenylmethylsulfonyl fluoride - MF microfilament - MT microtubule - Rh-Phal rhodaminelabeled phalloidin     

Irradiance-dependent regulation of gravitropism by red light in protonemata of the moss Ceratodon purpureus

Apical cells of protonemata of the moss Ceratodon purpureus (Hedw.) Brid. are negatively gravitropic in the dark and positively phototropic in red light. Various fluence rates of unilateral red light were tested to determine whether both tropisms operate simultaneously. At irradiances ≥140 nmol m⁻² s⁻¹ no gravitropism could be detected and phototropism predominated, despite the presence of amyloplast sedimentation. Gravitropism occurred at irradiances lower than 140 nmol m⁻² s⁻¹ with most cells oriented above the horizontal but not upright. At these low fluence rates, phototropism was indistinct at 1 g but apparent in microgravity, indicating that gravitropism and phototropism compete at 1 g. The frequency of protonemata that were negatively phototropic varied with the fluence rate and the duration of illumination, as well as with the position of the apical cell before illumination. These data show that the fluence rate of red light regulates whether gravitropism is allowed or completely repressed, and that it influences the polarity of phototropism and the extent to which apical cells are aligned in the light path. Received: 19 January 1999 / Accepted: 19 March 1999     

Organization of the actin cytoskeleton during pollen development inGasteria verrucosa (Mill.) H. Duval visualized with rhodamine-phalloidin

The three-dimensional organization of the microfilamental cytoskeleton of developingGasteria pollen was investigated by light microscopy using whole cells and fluorescently labelled phalloidin. Cells were not fixed chemically but their walls were permeabilized with dimethylsulphoxide and Nonidet P-40 at premicrospore stages or with dimethylsulphoxide, Nonidet P-40 and 4-methylmorpholinoxide-monohydrate at free-microspore and pollen stages to dissolve the intine.Four strikingly different microfilamentous configurations were distinguished. (i) Actin filaments were observed in the central cytoplasm throughout the successive stages of pollen development. The network was commonly composed of thin bundles ramifying throughout the cytoplasm at interphase stages but as thick bundles encaging the nucleus prior to the first and second meiotic division. (ii) In released microspores and pollen, F-actin filaments formed remarkably parallel arrays in the peripheral cytoplasm. (iii) In the first and second meiotic spindles there was an apparent localization of massive arrays of phalloidin-reactive material. Fluorescently labelled F-actin was present in kinetochore fibers and pole-to-pole fibers during metaphase and anaphase. (iv) At telophase, microfilaments radiated from the nuclear envelopes and after karyokinesis in the second meiotic division, F-actin was observed in phragmoplasts.We did not observe rhodamine-phalloidin-labelled filaments in the cytoplasm after cytochalasin-B treatment whereas F-actin persisted in the spindle. Incubation at 4° C did not influence the existence of cytoplasmic microfilaments whereas spindle filaments disappeared. This points to a close interdependence of spindle microfilaments and spindle tubules.Based on present data and earlier observations on the configuration of microtubules during pollen development in the same species (Van Lammeren et al., 1985, Planta165, 1-11) there appear to be apparent codistributions of F-actin and microtubules during various stages of male meiosis inGasteria verrucosa.Abbreviation DMSO dimethylsulfoxide     

Novel mutant phenotypes of a dark-germinating mutant<Emphasis Type="Italic"> dkg1</Emphasis> in the fern<Emphasis Type="Italic"> Ceratopteris richardii</Emphasis>

The mutant dark-germinating 1 (dkg1) of the fern Ceratopteris richardii was originally characterized by two phenotypes, germination in the dark and inhibition of germination by light. In this work, we examined whether other phenotypes are present in the gametophytic generation of the dkg1 mutant. Although dkg1 prothalli grown in darkness were elongated as in the case of the wild type, some developmental processes were found to proceed even in complete darkness: (1) the apical and subapical zones developed largely by forming a lateral meristem; (2) asymmetric cell division for rhizoid differentiation occurred in the subapical elongation zone; (3) an archegonium was formed in the proximity of the meristem; and (4) chloroplast relocation could occur without de novo protein synthesis. Furthermore, these processes were shown to be under the control of phytochrome in the wild-type gametophytes on the basis of red/far-red reversibility. These results indicate that the DKG1 gene is pleiotropic and is involved in several phytochrome-mediated responses in the gametophyte development of C. richardii.     

Reorientation of microtubules at the outer epidermal wall of maize coleoptiles by phytochrome,blue-light photoreceptor,and auxin

Summary The effects of red and blue light on the orientation of cortical microtubules (MTs) underneath the outer epidermal wall of maize (Zea mays L.) coleoptiles were investigated with immunofluorescent techniques. The epidermal cells of dark-grown coleoptiles demonstrated an irregular pattern of regions of parallel MTs with a random distribution of orientations. This pattern could be changed into a uniformly transverse MT alignment with respect to the long cell axis by 1 h of irradiation with red light. This response was transient as the MTs spontaneously shifted into a longitudinal orientation after 1–2 h of continued irradiation. Induction/reversion experiments with short red and far-red light pulses demonstrated the involvement of phytochrome in this response. In contrast to red light, irradiation with blue light induced a stable longitudinal MT alignment which was established within 10 min. The blue-light response could not be affected by subsequent irradiations with red or far-red light indicating the involvement of a separate blue-light photoreceptor which antagonizes the effect of phytochrome. In mixed light treatments with red and blue light, the blue-light photoreceptor always dominated over phytochrome which exhibited an apparently less stable influence on MT orientation. Long-term irradiations with red or blue light up to 6 h did not reveal any rhythmic changes of MT orientation that could be related to the rhythmicity of helicoidal cell-wall structure. Subapical segments isolated from dark-grown coleoptiles maintained a longitudinal MT arrangement even in red light indicating that the responsiveness to phytochrome was lost upon isolation. Conversely auxin induced a transverse MT arrangement in isolated segments even in blue light, indicating that the responsiveness to blue-light photoreceptor was eliminated by the hormone. These complex interactions are discussed in the context of current hypotheses on the functional significance of MT reorientations for cell development.Abbreviations MT cortical microtubule - P_r, P_fr red and far-red absorbing form of phytochrome     

Branch formation induced by microbeam irradiation ofAdiantum protonemata

Branches were induced in centrifugedAdiantum protonemal cells by partial irradiation with polarized red light. Nuclear behavior and microtubule pattern change during branch formation were investigated. A branch formed at any part where a red microbeam was focused along a long apical cell. The nucleus moved towards the irradiated area and remained there until a branch developed. The pattern of microtubules changed from parallel to oblique at the irradiated area and then a transverse arrangement of microtubules appeared on both sides of the area. It appeared as if the nucleus was suspended between two microtubule rings. This nuclear behavior and the changes in microtubule pattern were different from those observed during branch formation under whole cell irradiation. From the results of this work we suggest that there is an importance for precise control of experimental conditions.     

Action spectra for the inhibition of growth in radish hypocotyls

In etiolated seedlings of Raphanus sativus L. the inhibition of hypocotyl elongation by continuous light showed a major bimodal peak of action in the red and far-red, and two minor peaks in the blue regions of the spectrum. It is argued that, under conditions of prolonged irradiation, phytochrome is the pigment controlling the inhibition of hypocotyl elongation by red and far-red light, but that its mode of action in far-red is different from that in red. A distinct pigment is postulated for blue light.Abbreviations B blue - FR far red - G green - R red - HIR high irradiance reaction - P_r and P_fr red and far red absorbing forms of phytochrome - R red     

Cryptic species in the fern Ceratopteris thalictroides (L.) Brongn. (Parkeriaceae). I. Molecular analyses and crossing tests

For the taxonomic revision of the problematic species Ceratopteris thalictroides, molecular analyses and crossing tests were conducted for 16 sources in the world. An analysis of allozyme composition of five enzymes revealed the presence of three intraspecific entities, which were called the south type, the north type, and the third type. An analysis of the nucleotide sequences of chloroplast DNA also distinguished the same entities. Crossing tests showed that the south type was completely cross-sterile with the other two types, and that the other two were considerably cross-sterile with each other. These results suggest that the three entities should be regarded as different biological species. Although the south type and the other two meet in several regions, complete cross-sterility between them seems to sustain their genetic distinctiveness in spite of occasional crossing. The results from the present study suggest that widely distributed fern species are apt to comprise several cryptic species. Received: August 1, 2001 / Accepted: November 1, 2001