共查询到20条相似文献,搜索用时 78 毫秒
1.
摘要 目的:探讨急性淋巴细胞白血病(ALL)患儿血清颗粒蛋白前体(PGRN)、X-C基序趋化因子配体1(XCL1)与危险度分层和预后的关系。方法:选取2018年1月~2020年1月我院收治的102例ALL患儿为ALL组,根据危险度分层分为低危组27例、中危组42例、高危组33例,根据生存结局分为死亡组和存活组,另选取同期50名于我院体检健康儿童为对照组。采用酶联免疫吸附法检测血清PGRN、XCL1水平。采用多因素Logistic回归分析ALL患儿死亡的影响因素,受试者工作特征(ROC)曲线分析血清PGRN、XCL1水平对ALL患儿死亡的评估价值。结果:与对照组比较,ALL组血清PGRN、XCL1水平升高(P<0.05)。低危组、中危组、高危组血清PGRN、XCL1水平依次升高(P<0.05)。随访2年,102例ALL患儿死亡率为34.31%(35/102)。单因素分析显示,白细胞计数(WBC)、混合谱系白血病(MLL)重排、危险度分层、PGRN、XCL1为ALL患儿死亡的影响因素(P<0.05)。多因素Logistic回归分析显示,WBC≥50×109/L、MLL重排阳性、危险度分层高危和PGRN、XCL1升高为ALL患儿死亡的独立危险因素(P<0.05)。ROC曲线分析显示,血清PGRN联合XCL1评估ALL患儿死亡的曲线下面积(AUC)大于PGRN、XCL1单独评估。结论:ALL患儿血清PGRN、XCL1水平升高,与危险度分层和预后有关,血清PGRN联合XCL1评估ALL患儿预后的价值较高,可能成为ALL患儿预后辅助评估指标。 相似文献
2.
摘要 目的:分析血清载脂蛋白C1(APOC1)、乳酸脱氢酶(LDH)及炎性感染指标在难治性支原体肺炎患儿中的表达水平及其与肺通气功能、预后效果的相关性。方法:选择我院自2019年1月至2022年12月接诊的130例难治性支原体肺炎患儿作为观察组,另选同期在我院体检且结果正常的儿童作为对照组。检测两组入选者血清APOC1、LDH及炎性感染指标[C反应蛋白(CRP)、白细胞介素-6(IL-6)]表达水平、肺通气功能指标[用力肺活量(FVC)、第1秒用力呼气容积(FEV1)、呼气峰流速(PEF)];观察组患儿均严格按照《儿童社区获得性肺炎诊疗规范(2019年版)》接受治疗,通过ROC曲线下面积(AUC)评价血清APOC1、LDH及炎性感染指标对难治性支原体肺炎患儿预后不良的预测效能。结果:观察组血清APOC1、LDH、CRP、IL-6表达水平均高于对照组(P<0.05);在观察组患儿中,重症组血清APOC1、LDH、CRP、IL-6表达水平均高于轻症组(P<0.05);经Pearson相关性分析,难治性支原体肺炎患儿血清APOC1、LDH、CRP、IL-6表达水平均与FVC、FEV1、PEF呈负相关(P<0.05);经多因素Logistic回归分析,难治性支原体肺炎患儿血清APOC1、LDH、CRP、IL-6均是其预后不良的独立预测因素(P<0.05);经ROC曲线分析,难治性支原体肺炎患儿血清APOC1、LDH、CRP联合IL-6预测其预后不良的AUC为0.930。结论:血清APOC1、LDH及炎性感染指标在难治性支原体肺炎患儿中的表达水平明显升高,与其肺通气功能密切相关,其中APOC1、LDH、CRP联合IL-6预测预后不良的效能较好,值得进一步研究应用。 相似文献
3.
摘要 目的:探讨中性粒细胞与淋巴细胞比值(NLR)、25-羟维生素D3 [25-(OH)D3]、白细胞介素-6(IL-6)、降钙素原(PCT)与重症肺炎支原体肺炎(MMP)患儿免疫功能和预后不良的关系。方法:选取2019年2月至2021年12月我院收治的106例重症MMP患儿作为重症组,同期收治的101例轻症MMP患儿(轻症组)作为对照。检测外周血中性粒细胞计数、淋巴细胞计数、T淋巴细胞亚群以及血清25-(OH)D3、IL-6、PCT水平,计算NLR。分析NLR、25-(OH)D3、IL-6、PCT与T淋巴细胞亚群的相关性。重症MMP患儿治疗后随访半年,根据重症MMP患儿的预后情况分为预后良好组(75例)和预后不良组(31例),多因素Logistic回归分析影响重症MMP患儿预后的因素。结果:重症组NLR、IL-6、PCT水平,CD8+高于轻症组(P<0.05),25-(OH)D3水平、CD3+、CD4+、CD4+/CD8+低于轻症组(P<0.05)。NLR、IL-6、PCT水平与CD3+、CD4+、CD4+/CD8+呈负相关(P<0.05),与CD8+呈正相关(P<0.05);25-(OH)D3与CD3+、CD4+、CD4+/CD8+呈正相关(P<0.05),与CD8+呈负相关(P<0.05)。多因素Logistic回归分析显示:肺大片实变影、NLR(较高)、IL-6(较高)、PCT(较高)是重症MPP患儿预后不良的危险因素(P<0.05),25-(OH)D3(较高)是保护因素(P<0.05)。结论:重症MMP患儿NLR、IL-6、PCT水平升高,25-(OH)D3水平降低,且与细胞免疫功能低下以及预后不良有关,检测NLR、IL-6、PCT、25-(OH)D3有助于评估重症MMP患儿的预后。 相似文献
4.
摘要 目的:探讨肺炎支原体肺炎(MPP)患儿血清表面活性蛋白D(SP-D)、半乳糖凝集素-3(Gal-3)、C-C基序趋化因子配体5(CCL5)水平与炎症因子和预后不良的关系。方法:选取2020年1月~2021年1月我院收治的165例MPP患儿为MPP组,另选取54例健康儿童为对照组。采用ELISA法检测血清SP-D、Gal-3、CCL5、白细胞介素-6(IL-6)、IL-8水平,放射免疫法检测血清肿瘤坏死因子-α(TNF-α)水平。采用Pearson/Spearman相关系数分析MPP患儿血清SP-D、Gal-3、CCL5水平与IL-6、IL-8、TNF-α水平的相关性。通过多因素Logistic回归分析MPP患儿预后不良的影响因素。采用接受者操作特征(ROC)曲线分析血清SP-D、Gal-3、CCL5水平对MPP患儿预后不良的预测价值。结果:与对照组比较,MPP组患儿的血清SP-D、Gal-3、CCL5以及IL-6、IL-8、TNF-α水平升高(P<0.05)。MPP患儿的血清SP-D、Gal-3、CCL5水平与IL-6、IL-8、TNF-α水平均呈正相关(P<0.05)。血清SP-D、Gal-3、CCL5、IL-6、TNF-α水平较高、病变类型为大片状阴影、热程较长是MPP患儿预后不良的危险因素(P<0.05)。血清SP-D、Gal-3、CCL5三项联合预测MPP患儿预后不良的曲线下面积(AUC)为0.926,明显大于三项指标单独预测的0.842、0.794、0.771。结论:MPP患儿血清SP-D、Gal-3、CCL5水平升高,与炎症因子和预后不良密切相关,可作为MPP患儿预后不良的预测指标。 相似文献
5.
摘要 目的:探讨血清白细胞介素-6(IL-6)、C-反应蛋白与白蛋白比值(CAR)联合中性粒细胞与淋巴细胞比值(NLR)对新生儿呼吸窘迫综合征(NRDS)患儿病情和预后的评估价值。方法:选取2020年12月~2022年4月我院收治的102例NRDS患儿(NRDS组),根据病情严重程度分为轻度亚组23例、中度亚组38例、重度亚组41例,根据28 d治疗后结局分为死亡组37例和存活组65例,另选取同期57名健康新生儿(对照组)。检测血清IL-6、CAR、NLR,多因素Logistic回归分析NRDS患儿预后不良的影响因素,采用受试者工作特征(ROC)曲线分析血清IL-6、CAR、NLR对NRDS患儿预后不良的评估价值。结果:NRDS组血清IL-6、CAR、NLR均高于对照组(P均<0.001)。轻度、中度、重度亚组血清IL-6、CAR、NLR依次升高(P均<0.001)。死亡组血清IL-6、CAR、NLR高于存活组(P均<0.001)。多因素Logistic回归分析显示,胎龄和5min Apgar评分增加为NRDS患儿预后不良的独立保护因素,IL-6、CAR、NLR升高为独立危险因素(P均<0.05)。ROC曲线分析显示,血清IL-6、CAR、NLR联合评估NRDS患儿预后不良的曲线下面积(AUC)大于各指标单独评估(P均<0.05)。结论:NRDS患儿血清IL-6、CAR、NLR升高与病情加重和预后不良密切相关,血清IL-6、CAR联合NLR评估NRDS患儿预后不良的价值较高。 相似文献
6.
摘要 目的:探讨血清尿酸(UA)、白细胞介素-6(IL-6)、磷酸化Tau181(P-tau181)与阿尔茨海默病(AD)患者认知功能、日常生活能力和预后的关系。方法:选择2018年5月至2021年1月上海交通大学医学院附属第九人民医院与上海交通大学医学院附属第九人民医院黄浦分院收治的98例AD患者(AD组)和71例门诊体检的健康志愿者(对照组),检测血清UA、IL-6、P-tau181水平,使用简易智力状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)评估患者认知功能,Barthel指数(BI)评估患者日常生活能力。所有患者出院后随访1年,统计随访期间不良预后发生情况。Pearson相关性分析UA、IL-6、P-tau181与MMSE、MoCA、BI的相关性,多因素Logistic回归分析AD患者预后不良的危险因素。结果:AD组血清UA水平和MMSE、MoCA评分、BI低于对照组(P<0.05),IL-6、P-tau181水平高于对照组(P<0.05)。AD患者血清UA水平与MMSE、MoCA评分、BI呈正相关(P<0.05),IL-6、P-tau181水平与MMSE、MoCA评分、BI呈负相关(P<0.05)。多因素Logistic回归分析结果显示:长期卧床、AD痴呆阶段、高水平IL-6、高水平P-tau181是AD患者预后不良的危险因素(P<0.05),高水平UA是保护因素(P<0.05)。结论:AD患者血清UA水平降低,IL-6、P-tau181水平升高,且与认知功能障碍、日常生活能力降低以及预后不良有关,检测血清UA、IL-6、P-tau181可辅助评估AD病情和预后。 相似文献
7.
摘要 目的:探讨血清过氧化还原蛋白1(PRDX1)、成纤维细胞生长因子4(FGF4)、铁调素25(Hepc25)与急性缺血性脑卒中(AIS)患者病情严重程度和预后的关系。方法:选择2018年1月到2020年1月我院收治的73例AIS患者(AIS组)和同期于我院进行体检的56例健康者(对照组),根据入院当日美国国立卫生研究院卒中量表(NIHSS)评分将AIS组进一步分为轻症组(NIHSS评分<6分,21例)、中症组(6分≤NIHSS评分<13分,38例)、重症组(NIHSS评分≥13分,14例)。根据患者出院后改良Rankin量表(mRS)评分将其分为预后不良组(≥3分,18例)和预后良好组(0~2分,55例)。检测所有受试者血清PRDX1、FGF4、Hepc25水平,分析PRDX1、FGF4、Hepc25与NIHSS、mRS评分相关性,分析AIS患者预后的影响因素。结果:AIS组血清PRDX1、FGF4、Hepc25水平均高于对照组(P<0.05),重症组血清PRDX1、FGF4、Hepc25水平高于中症组和轻度症组(P<0.05),且中症组血清PRDX1、FGF4、Hepc25水平高于轻症组(P<0.05),预后不良组血清PRDX1、FGF4、Hepc25水平高于预后良好组(P<0.05)。AIS患者血清PRDX1、FGF4、Hepc25水平均与NHISS评分、mRS评分呈正相关(r=0.636、0.794、0.682;0.619、0.705、0.713,P<0.05)。单因素分析结果显示年龄、高血压、糖尿病、入院时NIHSS评分与AIS患者预后有关(P<0.05),多因素Logistic回归分析结果显示入院时高NIHSS评分,高血清PRDX1、FGF4、Hepc25水平是AIS患者预后不良的危险因素(P<0.05)。结论:AIS患者血清RDX1、FGF4、Hepc25水平明显升高,高水平RDX1、FGF4、Hepc25与AIS患者严重神经缺损和预后不良密切相关,可以作为AIS预后评估的辅助生物学指标。 相似文献
8.
摘要 目的:探讨慢性肺源性心脏病(CCP)患者血清白细胞介素-6(IL-6)、降钙素原(PCT)、D-二聚体(D-D)、N端B型脑钠肽前体(NT-proBNP)与心功能分级和预后的关系。方法:选取2020年1月~2021年5月合肥市第一人民医院全科医学科及呼吸与危重症科收治的170例CCP患者(CCP组),其中纽约心脏协会(NYHA)心功能分级Ⅱ级49例、Ⅲ级66例、Ⅳ级55例,根据1年后存活状况分为死亡组26例和存活组144例,另选取同期54名体检健康者(对照组)。收集CCP患者临床资料,采用酶联免疫吸附法检测血清IL-6、PCT、D-D、NT-proBNP水平。通过多因素Logistic回归分析CCP患者预后不良的影响因素,受试者工作特征(ROC)曲线分析血清IL-6、PCT、D-D、NT-proBNP水平对CCP患者预后不良的预测价值。结果:与对照组比较,CCP组血清IL-6、PCT、D-D、NT-proBNP水平升高(P<0.05)。Ⅱ级、Ⅲ级、Ⅳ级CCP患者血清IL-6、PCT、D-D、NT-proBNP水平依次升高(P<0.05)。单因素分析显示,死亡组NYHA心功能分级Ⅳ级、肺动脉收缩压、Tie指数和血清IL-6、PCT、D-D、NT-proBNP水平高于存活组,第1秒用力呼气容积(FEV1)占预计值%、FEV1/用力肺活量(FVC)、三尖瓣环收缩期位移低于存活组(P<0.05)。多因素Logistic回归分析显示,NYHA心功能分级Ⅳ级和肺动脉收缩压、IL-6、PCT、D-D、NT-proBNP升高为CCP患者预后不良的独立危险因素,FEV1占预计值%升高为独立保护因素(P<0.05)。ROC曲线分析显示,血清IL-6、PCT、D-D、NT-proBNP水平单独与联合预测CCP患者预后不良的曲线下面积分别为0.789、0.789、0.792、0.801、0.954,灵敏度分别为93.33%、66.67%、66.67%、86.67%、86.67%,特异度分别为46.67%、82.67%、85.33%、66.67%、94.67%。血清IL-6、PCT、D-D、NT-proBNP水平联合预测CCP患者预后不良的AUC大于单项预测(P<0.05)。结论:CCP患者血清IL-6、PCT、D-D、NT-proBNP水平升高可增加心功能严重程度并导致预后不良,可作为CCP患者预后不良的辅助预测指标,且四项指标联合检测具有更好的预测效能。 相似文献
9.
摘要 目的:分析慢性阻塞性肺疾病急性加重期(AECOPD)无创机械通气患者血清白细胞介素(IL)-13、IL-17、IL-37的变化并探讨其与患者预后不良的关系。方法:选取2019年1月~2022年2月我院收治的102例接受无创机械通气治疗的AECOPD患者(AECOPD组),根据预后情况分为预后不良亚组41例和预后良好亚组61例,另选取68例慢性阻塞性肺疾病稳定期(SCOPD)患者作为SCOPD组和57例体检健康者作为对照组。采用酶联免疫吸附法检测血清IL-13、IL-17、IL-37水平。采用单因素和多因素Logistic回归分析AECOPD无创机械通气患者预后不良的影响因素,受试者工作特征(ROC)曲线分析血清IL-13、IL-17、IL-37水平对AECOPD无创机械通气患者预后不良的预测价值。结果: 对照组、SCOPD组、AECOPD组血清IL-13、IL-17、IL-37水平依次升高(P<0.05)。预后不良亚组血清IL-13、IL-17、IL-37水平高于预后良好亚组(P<0.05)。单因素分析显示,预后不良亚组年龄大于预后良好亚组,心功能不全比例和COPD评估测试(CAT)评分、C反应蛋白高于预后良好亚组(P<0.05),而两组性别、体质指数、AECOPD病程、吸烟、糖尿病、高血压、冠心病、慢性肾功能不全、第1秒用力呼气容积/用力肺活量(FEV1/FVC)、白细胞计数、降钙素原组间对比无差异(P>0.05)。多因素Logistic回归分析显示,FEV1%增加为AECOPD无创机械通气患者预后不良的独立保护因素,年龄增加、CAT评分增加和IL-13、IL-17、IL-37升高独立危险因素(P<0.05)。ROC曲线分析显示,血清IL-13、IL-17、IL-37水平联合预测AECOPD无创机械通气患者预后不良的曲线下面积大于IL-13、IL-17、IL-37水平单独预测。结论:AECOPD无创机械通气患者血清IL-13、IL-17、IL-37水平升高与预后不良密切相关,血清IL-13、IL-17、IL-37可作为AECOPD无创机械通气患者预后评估的潜在生物学指标。 相似文献
10.
摘要 目的:探讨小儿危重病例评分(PCIS)联合脑脊液降钙素原(PCT)、白细胞介素-6(IL-6)对重症病毒性脑炎(SVE)患儿预后不良的预测价值。方法:回顾性分析2020年1月至2021年12月我院收治的104例SVE患儿的临床病历资料,根据儿童格拉斯哥预后量表(CGOS)将患儿分为预后不良组(CGOS评级1~4级,n=39)和预后良好组(CGOS评级5级,n=65)。采用PCIS对患儿病情严重程度进行评估,采用酶联免疫吸附法检测患儿脑脊液PCT和IL-6水平。采用多因素logistic回归分析SVE患儿预后不良的影响因素,绘制受试者工作特征(ROC)曲线评估PCIS联合脑脊液PCT及IL-6对预后不良的预测价值。结果:预后不良组意识障碍、惊厥、合并脏器损伤发生率、脑脊液白细胞计数、PCT、IL-6水平高于预后良好组(P<0.05),视频脑电图(VEEG)重度异常构成比大于预后良好组(P<0.05),预后不良组PCIS评分低于预后良好组(P<0.05)。多因素logistic回归分析结果显示:合并脏器损伤、VEEG重度异常、脑脊液白细胞计数(较高)、PCT(较高)和IL-6水平(较高)是SVE患儿预后不良的危险因素(OR=1.449、3.732、1.355、1.483、1.388,P<0.05),PCIS评分(较高)是SVE患儿预后不良的保护因素(OR=0.769,P<0.05)。PCIS评分、脑脊液PCT和IL-6预测预后不良的曲线下面积(AUC)为0.869,灵敏度、特异性分别为0.85、0.78,均高于单一指标检测。结论:PCIS、脑脊液PCT及IL-6均是SVE患儿预后的影响因素,联合检测有助于预测患儿预后不良发生风险,从而指导临床制定针对性诊疗方案以改善患儿预后。 相似文献
11.
12.
Pushkar Shejwalkar 《Inorganica chimica acta》2011,366(1):209-218
New sulfur derivatives of phosphoramidite ligands were synthesized and the impact of the sulfur unit on the spectroscopic properties of their rhodium and iridium complexes was investigated. The new ligands Bn2NPSCH2CH2Sa(P-Sa) (Bn = benzyl, 4), Bn2NPSCHCHSa(CH2)3CaH2(P-Sa)(Ca-Sa) (6) and Bn2NP(4-XC6H4OMe)2 (X = S, 7a; X = O, 7b) were converted to the rhodium and iridium complexes trans-[Rh(CO)Cl(L)2] (L = 4, 6, 7), [RhCl(COD)(L)] (L = 4, 6, 7), [IrCl(COD)(7a)] and [IrCl2Cp∗(6)]. For comparison, some phosphoramidite complexes of these formulations also were synthesized. The new metal complexes were spectroscopically analyzed. For the carbonyl complexes, the νCO IR stretching frequencies were lower than for the corresponding phosphite and phosphoramidite ligands. The 1JPRh coupling constants for the rhodium complexes with the new ligands were also smaller than for the respective phosphoramidite and phosphite complexes. Finally, the 1JPSe coupling constants of the selenides of the new ligands were lower than those of the phosphoramidite ligands but higher than for PPh3. The spectroscopic data reveal that the new thio ligands 4, 6 and 7a are more electron donating than phosphites and phosphoramidites but less electron donating than PPh3. 相似文献
13.
Astrocytes transport the monocarboxylate acetate, but synaptosomes do not. The reason for this is unknown, because both preparations express monocarboxylate transporters (MCT). The transport and metabolism of lactate, another monocarboxylate, was examined in these two preparations, and the results were compared to those for acetate. Lactate transport is more rapid in astrocytes than in synaptosomes, but of lower affinity (Kms of 17 and 4 mM, respectively). Lactate (0.2 mM) is metabolized to CO2 more rapidly in synaptosomes than in astrocytes (rates of 0.37 and 0.07 nmol x mg protein(-1) x min(-1), respectively). The reason for this is unclear, but cellular differences in lactate dehydrogenase isotype expression may be involved. Acetate is metabolized to CO2 more rapidly in astrocytes than in synaptosomes (rates of 0.43 and 0.02 nmol x mg protein(-1) x min(-1), respectively). This is likely due to cellular differences in the expression of monocarboxylate transporter subtypes. 相似文献
14.
Hand JW 《Progress in biophysics and molecular biology》2007,93(1-3):192-194
The first and second sessions of the Workshop focussed on the basics of ultrasound and infrasound, their applications in both industry and medicine, and metrology and protection standards for ultrasound applications. 相似文献
15.
16.
Gonzalez-Reimers E Rodriguez-Moreno F Martinez-Riera A Mas-Pascual A Delgado-Ureta E Galindo-Martin L Arnay-de la Rosa M Santolaria-Fernandez F 《Biological trace element research》1999,68(1):41-49
To elucidate accumulation of minerals in human iliac arteries with aging, the content of minerals was analyzed by inductively
coupled plasma atomic emission spectrometry. Bilateral common, internal, and external iliac arteries of 16 men and 8 women,
ranging ages from 65 to 93 yr, were examined. It was found that an extremely high accumulation of calcium and phosphorus occurred
in the common iliac artery at old age, being higher than that of the internal and external iliac arteries. It should be noted
that the accumulation of calcium and phosphorus is the highest in the common iliac artery among the human arteries examined
to date.
Regarding sexual differences, the content of calcium and phosphorus in the common and internal iliac arteries was higher in
women than in men, whereas their content in the external iliac artery was lower in women than in men. 相似文献
17.
18.
J. Morley M.A. Bray R.W. Jones D.H. Nugteren D.A. van Dorp 《Prostaglandins & other lipid mediators》1979,17(5):729-736
The ability of partially purified human and guinea-pig haematogenous cell populations, when cultured in vitro, to metabolise arachidonic acid (AA) has been studied. Supernatants from 24 hour cell culture have been subjected to analysis for products of AA metabolism by gas chromatography with electron-capture detection.The cell types studied were human peripheral blood monocytes (both glass adherent and non-adherent), neutrophils, eosinophils and leukemic leucocytes; thoracic duct lymphocytes and lung alveolar macrophages. From the guinea-pig, induced and non-induced macrophage or neutrophil enriched peritoneal exudate populations, lymph node cells, peritoneal eosinophils and peripheral blood platelets were examined. Supernatants were assayed for the presence of PGE2, PGD2, PGF2α, TXB2 and 6-keto-PGF1α. In all types studied PGE2 and TXB2 were the major products formed. The identification of PGE2 and TXB2 was confirmed by GC/MS with multiple ion monitoring.The results have been compared with other reports and their possible significance discussed in relation to the proposed role of prostaglandins as mediators and modulators in immunopathology. 相似文献
19.
Allergic asthma can be precipitated by many factors. For the atopic person, fungus, pollen, dust mites, cockroach antigens, and diesel exhaust are all agents that may trigger an allergic attack. Cytokines and chemokines are integral mediators of fungal asthma. From the earliest time points, they recruit and activate the cells required for the clearance of fungus as well as being critical factors involved in the immunopathology of this disease. In the final analysis, it is clear that these mediators can act to the benefit or the detriment of the host. 相似文献
20.
Sandra M. Vaz 《Archives of biochemistry and biophysics》2009,484(2):127-431
In spite of the many studies on protein modifications by reactive species, knowledge about the products resulting from the oxidation of protein-aromatic residues, including protein-derived radicals and their stable products, remains limited. Here, we compared the oxidative modifications promoted by peroxynitrite and myeloperoxidase/hydrogen peroxide/nitrite in two model proteins, ribonuclease (6Tyr) and lysozyme (3Tyr/6Trp). The formation of protein-derived radicals and products was higher at pH 5.4 and 7.4 for myeloperoxidase and peroxynitrite, respectively. The main product was 3-nitro-Tyr for both proteins and oxidants. Lysozyme rendered similar yields of nitro-Trp, particularly when oxidized by peroxynitrite. Hydroxylated and dimerized products of Trp and Tyr were also produced, but in lower yields. Localization of the main modified residues indicates that peroxynitrite decomposes to radicals within the proteins behaving less specifically than myeloperoxidase. Nitrogen dioxide is emphasized as an important protein modifier. 相似文献