异型兰的种子试管育苗

1植物名称异型兰(Chiloschista yunnanensisSchltr.)。2材料类别种子。3培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量、微量元素用量减半);(3)3g·L-1花宝1号(美国Hyponex公司,N:P:K=7:6:19);(4)MS+100mL·L-1椰子乳;(5)1/2MS+100mL·L-1椰子乳;(6)3g·L-1花宝1号+100mL·L-1椰子乳;(7)1/2MS+100mL·L-1椰子乳+1g·L-1蛋白胨;(8)1/2MS+100mL·L-1椰子乳+1g·L-1胰蛋白胨;(9)1/2MS+100mL·L-1椰子乳+1g·L-1酵母提取液。生根育苗培养基:(10)3g·L-1花宝1号+2g·L-1活性炭+100g·L-1香蕉汁;(11)3g·L-1花宝1号+2g·L-1活性炭…     

多花脆兰的离体快速繁殖

1 植物名称多花脆兰[Acampe rigida(Buch.-Ham.ex J.E.Smith)P.F.Hunt]. 2 材料类别种子. 3 培养条件种子萌发培养基:(1)MS;(2)1/2MS(大量元素用量减半);(3)KC[成份如下:MgSO4·7H2O 250 mg·L-1(单位下同);KH2PO425O;(NH4)2SO4 500; Ca(NO 3)2·2H2O 1 000 ;FeSO4·7H2O 25; MnSO4.4H2O 7.5;肌醇(C6H12O6)100;盐酸硫胺素(VB,)1; D-甘露糖醇12.7%];(4)1 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:16:9)+2 g.L-1花宝2号(美国Haponex公司产品,N:P:K=20:20:20);(5)MS+100 g·L-1香蕉汁;(6)1/2MS+100 g·L-1香蕉汁;(7)KC+100 g·L-1香蕉汁;(8)1 g·L-1花宝1号+2 g·L-1花宝2号+100g·L-1香蕉汁.原球茎增殖和分化成苗培养基:(9)1/2MS+6.BA 1.5+NAA 0.2+100 g·L-1香蕉汁;(10)1/2MS+6-BA 0.5+NAA 0.05+100 g·L-1香蕉汁;(11)1 g·L-1花宝1号+2 g·L-1花宝2号+6-BA 1.0+NAA0.5+100 g·L-1香蕉汁.     

虎杖的组织培养与快速繁殖

以虎杖茎段、叶柄、叶片为外植体探讨了愈伤组织诱导、分化和植株再生的条件,筛选出茎段生长培养基为1/2MS+BA1.0mg·L-1+KT0.5mg·L-1+NAA0.2mg·L-1,茎段、叶柄和叶片外植体愈伤组织诱导培养基为MS+BA1.0～2.0mg·L-1+KT0.2～0.5mg·L-1+NAA0.2～0.5mg·L-1或MS+BA2.0～3.0mg·L-1+KT0.2～0.5mg·L-1+2,4-D0.5mg·L-1;丛生芽诱导培养基为MS+BA2.0mg·L-1+KT0.5mg·L-1+IBA0.2mg·L-1+LH1000;不定根及根状茎诱导培养基为1/2MS+IBA0.2mg·L-1.     

白旗兜兰种子非共生萌发与试管苗快速繁育

对我国极小种群野生植物白旗兜兰的种子非共生萌发与试管苗快速繁育开展研究,对比了不同培养基、预处理、种子成熟度及光照对种子萌发的影响。白旗兜兰种子萌发的最适条件是授粉后270 d的种子,在1/4MS+10%椰汁培养基上,用1%Na OCl溶液处理种子40 min并在播种后的前4周进行黑暗培养。最适合原球茎转化幼苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+0.5 g·L-1活性炭+10%香蕉匀浆,而最佳幼苗生根壮苗的培养基是3.0 g·L-1花宝一号+1.0 mg·L-1NAA+1.0 mg·L-16-BA+0.5 g·L-1活性炭+10%香蕉匀浆。     

带叶兜兰的无菌播种和离体快速繁殖

1植物名称带叶兜兰(PaphiopedilumhirsutissimumPfitz)。2材料类别种子。3培养条件种子萌发培养基:(1)RE(Arditti1982);(2)VW;(3)1/4MS;(4)MS;(5)RE+活性炭2g·L-1;(6)RE;(7)RE+活性炭2g·L-1;(8)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)2.5g·L-1。原球茎增殖和分化成苗培养基:(9)RE+6-BA2.0mg·L-1(单位下同)+NAA0.2;(10)RE+活性炭2g·L-1+6-BA2.0+NAA0.2;(11)花宝1号1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+活性炭2g·L-1+6-BA2.0+NAA0.2。生根壮苗培养基:(12)RE+NAA1.0+活…     

蒙花忍冬的组织培养与快速繁殖研究

以金银花 蒙花 品系的茎段为外植体 ,于 MS和 B5附加不同激素配比的培养基上 ,探讨愈伤组织形成和丛生芽诱导及生根培养的条件 .诱导愈伤组织和丛生芽的培养基为 B5+BA2 .0 m g· L- 1 +KT0 .2～ 0 .5 mg· L- 1+IAA 0 .5～ 1 .0 mg· L- 1 +L H 1 0 0 0 m g· L- 1 ,继代增殖培养的培养基为 B5+BA 2 .0 m g· L- 1 +KT 0 .5 m g·L- 1 +IAA1 .0 m g· L- 1 +L H1 0 0 0 m g· L- 1和 B5+BA2 .0 mg· L- 1 +KT0 .36 m g· L- 1 +IAA1 .0 mg· L- 1+CH 1 0 0 0 m g· L- 1 +1 / 2 MS :大量元素 ,生根培养基为 1 / 2 MS +IBA 0 .2 mg· L- 1 +L H 1 0 0 0 mg·L- 1 .结果表明 L H和 CH在金银花愈伤组织和丛生芽诱导方面具明显的作用     

细茎石斛的快速繁殖和试管开花诱导

1植物名称细茎石斛[Dendrobiummoniliforme(L.)Sw.]。2材料类别野生植株的成熟种子。3培养条件种子萌发培养基:(1)1/2MS+NAA0.5mg·L-1(单位下同);增殖分化培养基:(2)1/2MS+NAA0.4;生根壮苗培养基:(3)1/2MS+NAA0.2+6-BA2.0;试管开花预处理培养基:(4)1/2MS+TDZ0.05,(5)1/2MS+PP3330.40+ABA1.0,预处理90d;试管开花培养基:(6)MS(1/6N,2P,2K)+PP3331.0+TDZ0.1。每个处理20株,重复3次,150d内统计花芽诱导率(花芽数/植株数)和正常花开放率(正常花开放数/植株数)。以上培养基均附加4%蔗糖、10%(W/V)香蕉汁、8g·L-1琼脂、0.1g·…     

土人参茎尖培养和植株再生

1　植物名称　土人参 (Talinumpaniculatum)。2　材料类别　实生苗之茎尖 ,种子取自本校花圃。3　培养条件　种子萌发培养基 :( 1 ) 1 /2MS +0 .7%琼脂 + 2 %蔗糖。丛生芽诱导培养基 :( 2 )MS + 6 BA 0 .5mg·L- 1 (单位下同 ) ;( 3)MS +NAA0 .5 + 6 BA 2 .0 ;( 4 )MS +NAA 0 .5 +KT 2 .0 ;( 5 )MS+NAA 0 .5 + 6 BA 3.0。丛生芽增殖培养基 :( 6)MS + 6 BA 2 .0 ;( 7)MS +NAA 0 .0 5 + 6 BA 3.0。生根培养基 :( 8) 1 /2MS +NAA 2 .0。上述 ( 2 )～ ( 8)培养基均附加 30 g·L- 1 蔗糖、7g·L- 1 琼脂 ,pH 5 .8。培养室温…     

火焰兰的种子培养和试管育苗

1植物名称火焰兰(Renanthera CoCCinea). 2材料类别种子. 3培养条件种子萌发培养基:(1)VW;(2)VW 100 mL·L-1椰子乳;(3)KC;(4)KC 100 mL·L-1椰子乳(5)1/2MS;(6)MS.生根育苗培养基:(7)3 g·L-1花宝1号(美国Haponex公司产品,N:P:K=7:6:19) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA;(8)1 g·L-1花宝1号 1 g·L-1花宝2号(N:P:K=20:20:20) 2 g·L-1蛋白胨 2 g·L-1活性炭 0.5 mg·L-1NAA 0.2 mg·L-16-BA.以上培养基均附加1.5%蔗糖、0.6%琼脂,pH 5.2～5.4.培养温度为(25±2)℃,光照度1 500～2000lx,光照时间12 h·d-1.     

羚羊石斛的离体快速繁殖

1植物名称羚羊石斛(DendrobiumcochliodesSchltr.)。2材料类别种子。3培养条件萌发培养基:(1)VW;(2)VW+椰子乳;(3)1/2MS;(4)1/2MS+椰子乳;(5)N6;(6)N6+椰子乳;(7)N6+椰子乳+活性炭2g·L-1。继代增殖和分化成苗培养基:(8)N6+椰子乳+6-BA1.0mg·L-1(单位下同)+NAA0.2;(9)N6+椰子乳+6-BA0.2+NAA0.1;(10)N6+椰子乳+6-BA0.2+NAA0.1+活性炭2g·L-1;(11)花宝1号(美国Haponex公司产品,N∶P∶K=7∶6∶19)1.5g·L-1+花宝2号(美国Haponex公司产品,N∶P∶K=20∶20∶20)1.5g·L-1+椰子乳+6-BA0.2+NAA0.1。生根壮苗培养基:(12)N6+NA…     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.