Investigation of the First Case of Dengue Virus Infection Acquired in Western Australia in Seven Decades: Evidence of Importation of Infected Mosquitoes?

In October 2013, a locally-acquired case of dengue virus (DENV) infection was reported in Western Australia (WA) where local dengue transmission has not occurred for over 70 years. Laboratory testing confirmed recent DENV infection and the case demonstrated a clinically compatible illness. The infection was most likely acquired in the Pilbara region in the northwest of WA. Follow up investigations did not detect any other locally-acquired dengue cases or any known dengue vector species in the local region, despite intensive adult and larval mosquito surveillance, both immediately after the case was notified in October 2013 and after the start of the wet season in January 2014. The mechanism of infection with DENV in this case cannot be confirmed. However, it most likely followed a bite from a single infected mosquito vector that was transiently introduced into the Pilbara region but failed to establish a local breeding population. This case highlights the public health importance of maintaining surveillance efforts to ensure that any incursions of dengue vectors into WA are promptly identified and do not become established, particularly given the large numbers of viraemic dengue fever cases imported into WA by travellers returning from dengue-endemic regions.     

Earth's Oldest (∼ 3.5 Ga) Fossils and the `Early Eden Hypothesis': Questioning the Evidence

We question the biogenicity of putative bacterial and cyanobacterial 'microfossils' from 3465 Ma Apex cherts of the Warrawoona Group in Western Australia. They are challenged on the basis of integrated multidisciplinary evidence obtained from field and fabric mapping plus new high-resolution research into their context, sedimentology, filament morphology, 'septation' and arrangement. They cannot be distinguished from (and are reinterpreted as) secondary artefacts of amorphous carbon that formed during devitrification of successive generations of carbonaceous hydrothermal dyke vein quartz. Similar structures occur within associated carbonaceous volcanic glass. The null hypothesis of an abiotic or prebiotic origin for such ancient carbonaceous matter is sustained until mutually supporting contextural, morphological and geochemical evidence for a bacterial rather than abiotic origin is forthcoming.     

Behaviour of Dinosaurian Track-Makers in the Winton Formation (Cretaceous,Albian–Cenomanian) at Lark Quarry,Western Queensland,Australia: Running or Swimming?

A remarkable assemblage of dinosaur trackways in the Winton Formation (Albian–Cenomanian) at Lark Quarry, in western Queensland, Australia, has long been regarded as evidence of a stampede involving small theropods, whose tracks were classified in the ichnogenus Skartopus, and small ornithopods, whose tracks represented a second ichnogenus, Wintonopus. However, one recently-published study has claimed that existing interpretation of Lark Quarry is incorrect: it maintains that all the track-makers were ornithopods, that the ichnogenus Skartopus is a variant form of Wintonopus, and that most of the Lark Quarry track-makers were not running but more probably swimming downstream in a current of water. Those iconoclastic claims are investigated here and shown to be untenable. They derive from overgeneralized interpretation of the Lark Quarry track assemblage and invoke ad hoc auxiliary hypotheses which are either untestable or demonstrably incorrect. Closer inspection of the evidence underpinning those claims corroborates the existing interpretation of Lark Quarry as the site of a dinosaurian stampede and confirms the validity of the original distinction between theropod tracks (Skartopus) and ornithopod tracks (Wintonopus).     

Legacy of Land-Use Evident in Soils of Western Australia’s Wheatbelt

Native regeneration of old-fields in the wheat-growing region of Western Australia is slow to non-existent. We compared the physio-chemical properties of three old-fields and three woodland remnants adjacent to each old-field to determine if current soil conditions are a barrier to regeneration. Of the variation in soil properties, 17.4% was described by spatial structure, 5.4% by land use (cultivation time and time since abandonment), 3.9% by soil type and 2.6% by vegetation and leaf litter cover. There were differences in individual soil properties between old-field and remnant soils at two sites. Soil compaction, probable erosion as evident by a higher percentage of coarse fragments, increased Colwell phosphate that we interpret as fertiliser residue and reduced organic carbon were evident in one old field, after 60 years of cultivation and 14 years abandonment. Increased Colwell phosphate was evident at the second old field, despite only 1 year in cultivation and 45 years in recovery. The third site showed no evidence of its 4 year period in cultivation 43 years ago despite similar farming practices and soil type to the second. Phosphate fertiliser residues could account for the dominance of non-native annual grasses in the re-assembly of wheatbelt old-fields.     

Further Evidence for the Formation in vivo of Phosphopeptide in the Intestinal Lumen from Dietary β-Casein

The occurrence of a new brassinosteroid of (22S,24R)-3β,22-dihydroxy-5α-ergostan-6-one, named cathasterone, was demonstrated by a GC-MS analysis in cultured cells of Catharanthus roseus. Its endogenous level was in the range of 2–4 ng/g fw, similar to those of brassinolide and castasterone. A feeding experiment with a deuterium-labeled substrate revealed that cathasterone was converted to teasterone and typhasterol. This is the first report of the natural occurrence of cathasterone as a brassinosteroid being the biosynthetic precursor of teasterone.     

Biomarkers of exposure in fish inhabiting the Swan–Canning Estuary, Western Australia – a preliminary study

The estuarine portion of the Swan–Canning riversystem runs through the centre of Perth,Western Australia's capital city, with apopulation of approximately 1.4 million people. Little is known about impact of chemicalsentering the estuary via road runoff andstormwater drains on biota inhabiting thesystem. Black bream (Acanthopagrusbutcheri) were collected from seven sites inthe Swan–Canning estuary during August andSeptember 2000, at the end of the winter (wet)season. Serum sorbitol dehydrogenase (s-SDH)was unaffected by the sex of the fish and nosignificant differences were observed betweenthe sites indicating that the measuredethoxyresorufin-O-deethylase (EROD)activity was not hindered by hepatic tissuedamage. The black bream were in an advancedstage of gonad maturation, which affected ERODhepatic activity with lower EROD activity infemale compared to male fish. EROD activityand bile metabolite levels were significantlyhigher at the site closest to the Perth CentralBusiness District, while most downstream sitewas the least impacted, which may be due totidal flushing of the lower estuary by marinewaters. The ratio of naphthalene-type tobenzo(a) pyrene (B(a)P)-typemetabolites suggests that the source ofpetroleum hydrocarbons within the river systemis a mixture polycyclic aromatic hydrocarbons(PAHs) from pyrolytic origin and from unburntfuels. Biomarker levels in the black breamindicate that major roads and drains aresignificant contributors of mixed functionoxygenase (MFO) inducing chemicals includingpolycyclic aromatic hydrocarbons (PAH) into theSwan–Canning estuary and that there is noupstream or downstream gradient in biomarkerresponse.     

Evidence of plant–insect interaction in the Early Cretaceous Flora from the Crato Formation,Araripe Basin,Northeast Brazil

The analysis of insect-plant interaction can be provide paleoecological and paleoenvironmental important data for understanding the co-evolution between plants and insects. Since the appearance of the first evidence of leaves damaged by insects, these organisms have evolved together. In the Araripe Basin, the Crato Formation stands out by having abundance and diversity of fossils species of plants and insects. In this work they are documented interaction records in specimens of ferns, gymnosperms and angiosperms, showing a wide variety of types of interactions. Here we analyze 56 fossil specimens from the collections of the Museum of Paleontology and the Laboratory of Paleontology of the Universidade Regional do Cariri. The types of damage identified in this study is insect galls, leaf margin feeding (herbivory), leaf mines, oviposition of insects and skeletonization, which are present in 19 specimens. This analysis in search for evidences of plant–insect interaction contributes with new interaction patterns for the Crato Formation. Although the low sample rate, the new registers for plant-insect association were compared to records from different cretaceous basins. This suggests new possibilities in the studies for ecological relations and coevolutionary plant-insect during the Cretaceous. In addition, a new type of damage is identified in a pteridophyte.     

Peculiarities of Formation of the Wakefulness—Sleep Cycle in Ontogenesis of Rats

Electrophysiological changes in the wakefulness—sleep cycle were studied in early postnatal ontogenesis of rat pups. EEG was recorded and its spectral power was determined, as well as spatial-temporal synchronization between the brain cortex zones (visual and sensomotor) and hippocampus in the process of sleep at various periods of ontogenesis. These data were compared with the literature data on studying of cytoarchitectonics and ultrastructure of rat neocortex as well as on formation of neuronal activity and maturation of transmitter systems at the same periods. Based on time of formation of interneuronal and interstructural connections and of maturation of transmitter systems, 3 stages of functional development of sleep in ontogenesis were identified: the first stage—undifferentiated sleep, the interneuronal connections are absent; the second stage—partially differentiated sleep, interneuronal connections function and control from subcortical structures appears; the third stage—differentiated sleep, clear division into sleep phase, additional control from all three transmitter systems: noradrenergic, cholinergic, and serotoninergic, action of the latter providing inhibitory mechanisms in CNS.__________Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 2, 2005, pp. 154–159.Original Russian Text Copyright © 2005 by Titkov, Aristakesyan, Oganesyan.     

Shifts in the seasonal distribution of deaths in Australia, 1968–2007

Studies in temperate countries have shown that both hot weather in summer and cold weather in winter increase short-term (daily) mortality. The gradual warming, decade on decade, that Australia has experienced since the 1960s, might therefore be expected to have differentially affected mortality in the two seasons, and thus indicate an early impact of climate change on human health. Failure to detect such a signal would challenge the widespread assumption that the effect of weather on mortality implies a similar effect of a change from the present to projected future climate. We examine the ratio of summer to winter deaths against a background of rising average annual temperatures over four decades: the ratio has increased from 0.71 to 0.86 since 1968. The same trend, albeit of varying strength, is evident in all states of Australia, in four age groups (aged 55 years and above) and in both sexes. Analysis of cause-specific mortality suggests that the change has so far been driven more by reduced winter mortality than by increased summer mortality. Furthermore, comparisons of this seasonal mortality ratio calculated in the warmest subsets of seasons in each decade, with that calculated in the coldest seasons, show that particularly warm annual conditions, which mimic the expected temperatures of future climate change, increase the likelihood of higher ratios (approaching 1:1). Overall, our results indicate that gradual climate change, as well as short-term weather variations, affect patterns of mortality.     

New Discoveries of Phaeophycean Fossils in the Early Cambrian, Haikou,Kunming,Yunnan,Southwest China

The paper deals with the new phaeophycean fossils in the Early Cambrian from Chengjiang Biota at Ercai Village of Haikou, Kunming, Yunnan, Southwest China. Three new taxa, Punctariopsis latifolia gen. et sp. nov., P.simplex gen. et sp. nov. and Vendotaenia cf. antiqua Gnilovskaya are described here in detail. These new findings show that the Chengjiang Biota is high in species diversity of fossil algae and might offer new evidence for a better understanding of the Cambrian explosive biological evolution. The fossil genus described here is found to have a close relationship with the living Punctaria based on similar morphology. The results suggest that the Chengjiang Biota in Haikou area of Kunming was living in an intertidal and subtidal seawater environment.     

Recent sedimentological conditions in the delta of the river Kyrönjoki,Western Finland

The estuary of the River Kyrönjoki was echo sounded and, on the basis of these results the delta was delimited and areas of erosion and accumulation were separated. Samples of the sediment surface (0–2 cm) from 65 sites in the estuary were analysed for organic content, C, N, P, Ca, Fe, Mn, Pb, Cu, Zn, Cd and Hg. On the basis of the chemical analyses the delta was divided into two parts according to the sedimentary conditions: (I) In the inner part, where acid fresh water of c. pH 5.5 from the river is predominant, the conditions were reducing. The Fe/Mn ratio of the sediment was high. The heavy metal content was usually low. The Hg content in the organic compounds of the sediment was, due to the acidity, higher than in the outer part. (II) In the outer part of the delta, where neutral brackish water of pH c. 7.0 dominates, the sedimentary conditions were mostly oxidizing. The Fe/Mn ratio was very low. The heavy metal and organic content was mostly high in the accumulation areas. This is because the fine-grained allochthonous material, which has a high heavy metal and organic content, flocculates and precipitates when it reaches the brackish water of the Gulf of Bothnia, and the salinity and the pH are higher.     

Mutagen Formation in the Nitrite—Piperic Acid Reaction

A morphological mutant of Neurospora crassa, which showed great changes in cell wall β-glucan structures, was obtained. The mutant lacked spore-forming ability. Chemical analysis indicated that the mutant cell walls had more carbohydrates and less proteins than the wild type. In the structural polymers of cell walls, heteroglycan and chitin were not apparently changed in their sugar composition and structures. On the other hand, the alkali-soluble β-glucan of this mutant showed significant changes in the chemical structure, particularly, the number and length of branches. The mutant glucan had about 2.5 times as many branches as that from wild type and the number of 1,3-linked glucose residues was greatly reduced.     

The role of bryozoans in fossil reefs—an example from the Middle Devonian of the Western Sahara

Stenolaemate bryozoans with their stable calcitic skeletons play a significant role in reef building. In the Middle Devonian Sabkhat Lafayrina reef complex (Western Sahara), bryozoans are abundant and diverse. Although they do not form part of the principal framework of reefs, bryozoans are involved significantly in reef growth, especially in the initial stage. In this way, bryozoans are important with respect to initiating reef growth. They contribute greatly to sediment stabilization, making it possible for principal reef builders to grow on hardened and stabilized substrates, and also play sediment-baffling and sediment-filling roles. The aim of this study is to document the diversity of bryozoans in a Middle Devonian reef complex and to estimate their potential for initiation and contribution to reef structures.     

Managing the droughts? Perception of resource management in the face of the drought hazard in Australia

The long history of drought occurrences in Australia is reflected not only in varying community perceptions of drought as a hazard, but also in the growing recognition of its role in Australian ecosystems. The history of drought occurrences and the evolution of private and governmental responses to drought are reviewed as the context for the expected modification of traditional official policies, due in 1990.     

On the Use of Distance in the Taxonomic Study of Critical Plant Groups—Case Studies of Western European Orchidaceae

Four distance coefficients are compared on four data sets composed of samples coming from western European populations of the genera Dactylorhiza, Orchis and Epipactis (Orchidaceae). The performance of the distance coefficients is evaluated through: (a) the quality of clusters obtained by five classical methods (as compared to a priori classification), (b) the Mantel statistic with respect to an a priori distance matrix resulting from previous knowledge, (c) the result obtained with the k-means method, and (d) principal coordinate diagrams. It appears that: (a) the Mahalanobis distance based on the pooled dispersion matrix performs best on the whole; (b) a distance based on the recently developed Common Principal Component model, used with a log transformation, also provides useful information and performs best on the largest data set; (c) the Gölz and Reinhard taxonomic distance, widely used among orchidologists, is attractive for its simplicity, yet good performance and the valuable information it provides, despite its theoretical shortcomings. A brief taxonomic discussion is made on the results obtained for the Dactylorhiza samples, especially about samples whose identification was in doubt.     

Mechanism of the Formation of γ-Glutamylpeptides in Glutamic Acid Fermentations

We have constructed phage cloning vectors from an actinophage, R4. A deletion derivative (R4 Δ22B) which had a BamHI linker inserted at the unique PvuII site was used to clone the thiostreptone resistant (tsr) gene derived from plasmid vector pIJ365. The tsr derivative obtained, R4Δ22B-tsr1, was shown to have the same level of thiostreptone resistance in lysogenized cells as that of pIJ365-carrying cells. Under the optimal conditions, R4Δ22B-tsr1 phage was lysogenized at a frequency of 5x10^-2 per infected phage. The usefulness of R4 phage derivatives for gene cloning is discussed.