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J. P. S. Oliveira O. Hakimi M. Murgu M. G. B. Koblitz M. S. L. Ferreira L. C. Cameron A. F. Macedo 《Plant Cell, Tissue and Organ Culture》2018,134(1):153-162
An increasing effort is dedicated to investigate the potential of native plants used in traditional medicine as a source of bioactive compounds for numerous industries. The bioprospection of the metabolome of medicinal and/or endangered plants has two important merits: confirming or revealing the biotechnological potential of that species, and assisting in its conservation. In addition, biotechnological techniques, such as tissue culture, are key strategies in conservation and multiplication of medicinal plants. This is the first in vitro development and non-targeted metabolome study by UPLC–QTOF–MSE of extracts from C. menthoides, an endangered medicinal plant. In vitro development investigation with a wide range of plant growth regulators resulted in maximum survival rate (81%) and the highest growth rate (1.74 cm?±?0.36) for plantlets cultured on Murashige and Skoog medium, supplemented with 1 µM gibberellic acid. Maximum rooting occurred on medium supplemented with 4.4 µM 6-benzyladenine, which also resulted in more leaves per plantlet (10.16?±?1.7). We developed a protocol that can be used for the clonal propagation and ex situ conservation of this species. In terms of metabolome analysis, a total of 107 metabolites from several classes were detected and identified in its hydrophilic extract (HE), including organic acids and derivatives, glucosinolates, terpenes, phenolic compounds as well as other polar metabolites. The metabolites in HE with the greatest signal intensity included the isoquinoline alkaloid magnoflorine; the coumaric acid rosmarinic acid; the steroid-cardanolide convallatoxin; two anthraquinones including the poorly investigated ventinone A. Several molecules identified here carry potential pharmacological benefits such as anti-inflammatory and anticancer applications. 相似文献
3.
Paolucci U Vigneau-Callahan KE Shi H Matson WR Kristal BS 《Omics : a journal of integrative biology》2004,8(3):209-220
Mathematical models that reflect the effects of dietary restriction (DR) on the sera metabolome may have utility in understanding the mechanisms of DR and in applying this knowledge to human epidemiological studies. Previous studies demonstrated both the feasibility of identifying biomarkers through metabolome analysis and the validity of our approach in independent cohorts of 6-month-old male and female ad libitum fed or DR rats. Cross-cohort studies showed that cohort-specific effects distorted the dataset. The present study extends these observations across the entire sample set, thereby validating our markers independently of specific cohorts. Metabolites originally identified in males were examined in females and vice-versa. DR's effect on the metabolome is partially gender-specific and is modulated by environmental factors. DR reduces inter-gender differences in the metabolome. Univariate statistical methods showed that 56/93 metabolites in the female samples and 39/93 metabolites in the male samples were significantly altered (using our previous cut-off criteria of p < or = 0.2) by DR. The metabolites modulated by DR present a wide spectrum of concentration, redox reactivity and hydrophilicity, suggesting that our serotype is broadly representative of the metabolome and that DR has broad effects on the metabolome. These studies, coupled with those in the preceding and following reports, also highlight the utility for consideration of the metabolome as a network of metabolites using appropriate data analysis approaches. The inter-cohort and inter-gender differences addressed herein suggest potential cautions, and potential approaches, for identification of multivariate biomarker profiles that reflect changes in physiological status, such as a metabolism that predisposes to increased risk of neoplasia. 相似文献
4.
为了研究雌雄异株植物构树(Broussonetia papyrifera(L.) Vent.)的性别差异,以其叶片为材料,采用石蜡切片法,对其主要解剖结构特征进行观察和比较。结果显示:(1)构树雌、雄株叶片解剖结构组成一致,均由表皮、叶肉组织和叶脉3部分组成。其上、下表皮均由一层细胞构成,具有较厚的角质层及丰富的表皮毛,叶肉中栅栏组织高度发达,此外,维管系统在叶中所占比例很大;(2)构树雌、雄株叶片细胞大小及厚度在各类型组织间存在一定差异,雄株叶片上表皮厚度、栅栏组织厚度、主脉维管束木质部厚度及维管束厚度均显著大于雌株叶片,且在栅海比、组织结构紧密度、组织结构疏松度和主脉维管束木质部面积占维管束的比例等方面也与雌株有极显著差异。研究结果表明构树叶片的解剖结构不仅具有旱生植物叶片的典型特征,而且还表现出明显的性别差异,这可能与构树雌、雄株的生殖分配有关。 相似文献
5.
Eiichiro Kinoshita 《Ecological Research》1986,1(2):157-171
The size-sex relationship and sexual differences of sixArisaema species native to Japan were investigated. The size-sex relationship showed almost the same pattern in all species. When
the plant was small in size, the sex expression was male, and sex expression changed from male to female as the plant grew
larger. Male ratios decreased rapidly around a critical size, but this critical size differed from one species to another.
Sexual differences were detected in reproductive structures and behavior, although no difference was detected in vegetative
structures. The stoutness, longevity and inner tissue of the scape showed remarkable differences between males and females,
and this difference was represented most clearly as the size-weight relationship. Earlier initiation of flowering in males
was also observed.
No difference was found in resource allocation to reproductive structures between male and female plants at the flowering
stage. However, a broad variation in the amount of resource allocation to reproductive structures was found at the fruiting
stage in female individuals, which was attributed to differences in the setting rate of mature fruits. 相似文献
6.
Plasma metabolome in young women following cranberry juice consumption were investigated using a global UHPLC-Q-Orbitrap-HRMS approach. Seventeen female college students, between 21 and 29 years old, were given either cranberry juice or apple juice for three days using a cross-over design. Plasma samples were collected before and after juice consumption. Plasma metabolomes were analyzed using UHPLC-Q-Orbitrap-HRMS followed by orthogonal partial least squares-discriminant analyses (OPLS-DA). S-plot was used to identify discriminant metabolites. Validated OPLS-DA analyses showed that the plasma metabolome in young women, including both exogenous and endogenous metabolites, were altered following cranberry juice consumption. Cranberry juice caused increases of exogenous metabolites including quinic acid, vanilloloside, catechol sulfate, 3,4-dihydroxyphenyl ethanol sulfate, coumaric acid sulfate, ferulic acid sulfate, 5-(trihydroxphenyl)-gamma-valerolactone, 3-(hydroxyphenyl)proponic acid, hydroxyphenylacetic acid and trihydroxybenzoic acid. In addition, the plasma levels of endogenous metabolites including citramalic acid, aconitic acid, hydroxyoctadecanoic acid, hippuric acid, 2-hydroxyhippuric acid, vanilloylglycine, 4-acetamido-2-aminobutanoic acid, dihydroxyquinoline, and glycerol 3-phosphate were increased in women following cranberry juice consumption. The metabolic differences and discriminant metabolites observed in this study may serve as biomarkers of cranberry juice consumption and explain its health promoting properties in human. 相似文献
7.
Costantini V Carraro C Bucci FA Simontacchi C Lacalandra GM Minoia P 《Animal reproduction science》2009,111(2-4):289-301
The neuroendocrine conditioning of reproduction in birds could perform a very important role in captive breeding, especially in endangered species. Whereas in domestic and wild mammals pharmacological reproductive conditioning is well developed, in birds an effective method is not available. The aim of this study was to test the influence of a new slow-release GnRH analogue (buserelin acetate) implant on the reproductive activity of the Budgerigar (Melopsittacus undulatus), used as model species for captive-bred endangered birds. The effects were assessed by looking at reproductive parameters (egg-laying rate, egg fertility rate) and measuring excreted sex steroid metabolite concentrations in male and female birds. Modification of reproductive parameters and steroid metabolites excretion patterns were observed among birds administered with a GnRH analogue implant and maintained under artificial photoperiod (group I; 16L:8D). Implanted birds showed higher rates of egg-laying, potentially a higher proportion of fertile eggs and higher excreted steroid metabolite concentrations than birds maintained under natural photoperiod (group II; 10L:14D) and birds maintained under artificial photoperiod (group III; 16L:8D). Thus, it is concluded that the new slow-release GnRH analogue implant may represent an innovative and practicable treatment to rapidly induce reproductive activity in the Budgerigar, and that excreted sex hormone metabolites detection permits to monitor male and female gonadal activity. 相似文献
8.
为了研究沙棘雌、雄株叶片的第二性征,本文采用石蜡切片法观察了沙棘雌、雄株叶片结构的差异。结果表明:(1)沙棘雌、雄株叶片均由表皮、叶肉和叶脉3部分组成,表皮均由1层细胞构成,表皮毛发达,上表皮有拟泡状细胞;叶肉栅栏组织与海绵组织分化明显。(2)雌株上表皮具更多的拟泡状细胞,其主脉韧皮部薄壁细胞及其下方的一些薄壁细胞含较多的后含物,下表皮的表皮毛更浓密;而雄株的叶片厚度、叶片上表皮厚度、栅栏组织厚度、栅栏组织厚度/海绵组织厚度均显著大于雌株,且其主脉维管束更发达。结果表明,沙棘雌雄株叶片解剖结构存在明显差异,这些差异是第二性征的表现,也是沙棘长期进化中形成的稳健的适应策略,可能有利于该物种的繁衍。 相似文献
9.
Maria Emilia Yamamoto Fabíola S. Albuquerque Nívia A. Lopes Eric S. Ferreira 《Acta ethologica》2008,11(2):95-99
Callitrichids are communal breeders that lack sexual dimorphism, and only a few studies have examined behavioral gender differences among them. The purpose of this study was to investigate gender differences in infant carrying in 16 captive and seven wild common marmoset groups. Our results showed that female–female twin pairs were carried significantly more often by fathers than were male–male and male–female pairs both in the wild and in captivity. We suggest these differences may be related to different reproductive potentials of male and female Callithrix jacchus and possibly to future breeding competition among females. 相似文献
10.
Douglas Maciel Reisla Oliveira Paulo Enrique Cardoso Peixoto 《Ethology : formerly Zeitschrift fur Tierpsychologie》2023,129(2):99-106
Sexually selected traits, such as male weapons, are highly variable in shape and size across species. However, little is known about the mechanisms that may govern this variation. Because males with greater investment in weapon size tend to win more fights, but also pay higher costs related to weapon development and maintenance, larger weapons should be expected only in species in which victory in male–male fights generate reproductive benefits that outweigh investment costs. Here, we hypothesized that the reproductive characteristics that increase the chances of winners to access females or to fertilize eggs will favor the evolution of larger weapons. To evaluate this hypothesis, we conducted a meta-analysis using arthropods as model organisms. To measure investment in weapon size, we gathered both Pearson correlation coefficient and the standardized (but non centralized) slope values for the relationship between weapon size and body size. We found that none of the reproductive characteristics we investigated was related to male weapon size. Thus, it seems that greater certainty of accessing a female or fertilizing female eggs with a victory does not modulate the investment in male weapon size. Perhaps the cost–benefit ratio between weapon size investment and reproductive success is not the main factor driving the variation in weapon size. 相似文献
11.
Teresa L. Dzieweczynski Meghan M. Walsh 《Ethology : formerly Zeitschrift fur Tierpsychologie》2011,117(1):10-18
The study of communication in a network setting has gained increasing popularity in recent years. While audience effects on aggressive interactions have been studied extensively, male–female interactions have often been overlooked. In addition, little is known about how reproductive status affects the nature of audience effects. Siamese fighting fish, Betta splendens, are a popular subject for communication network studies, but male–female interactions have not been explored in this setting. In this study, pairs of male and female Betta were presented with a male, female or no audience to determine whether the presence of an audience alters the behaviour of the interactants. Within these three audience types, there were four reproductive status conditions with receptivity indicated by nest presence for males and reproductive barring for females. It was predicted that male–female interactions would be affected by the presence of an audience, especially when both interactants are receptive as has been found in male–male interactions in this species. The results suggest that presence of an audience and reproductive status act in combination to influence male–female interactions, but only in interactant‐directed behaviours. Not all behaviours were equally affected by these factors. For example, while tail beats to the other interact were greatest when a female audience was present and both the interactants were receptive, this was not true for gill flaring. This study is among the first investigations into audience effects on male–female interactions including the first in Betta and suggests that courtship as well as aggression should be explored in a network setting. 相似文献
12.
绞股蓝雌雄种群觅源行为和繁殖对策比较 总被引:6,自引:0,他引:6
绞股蓝(Gynostemma pentaphyllum)雌雄异株,种群性比偏雄。作者利用比较生态学方法,从行为生态学角度探讨相同生境中绞股蓝雌雄种群的觅源行为和繁殖对策,得到如下初步结果和结论:(1)绞股蓝雄性种群的主枝生物量比显著大于雌性种群,这意味着雄性种群的营养繁殖投资显著增加,而两性种群在其它结构中的生物量分配无显著差异;(2)雌性种群的叶面积比和单位叶面积比雄性种群显著增加,这与两性种群 相似文献
13.
Using light and scanning electron microscopy, the internal male and female reproductive anatomy of the praying mantid Ciulfina klassi is identified and described. This is the first detailed study to investigate the internal reproductive morphology of any Mantodea. The female structures identified were (1) paired ovaries with primitive panoistic type ovarioles, (2) a single blind-ended spermatheca with secretory gland cells and surrounding layer of striated muscle, and (3) female accessory glands associated with the production of the ootheca (the egg casing). The male structures identified were (1) paired multi-tubular testes, in which different stages of spermatogenesis were observed, (2) tubular vasa deferentia, (3) seminal vesicles, (4) male accessory glands and (5) a single muscular ejaculatory duct. Knowledge of basic reproductive morphology can be used to infer function and so provide key information for future research into reproductive behavior and physiology in the Mantodea. 相似文献
14.
David G. Marneweck Andre Ganswindt Stephanie Rhodes Astrid Bellem Jocelyn Bryant Nadja Wielebnowski Fredrik Dalerum 《Acta theriologica》2013,58(2):223-232
Knowledge regarding the relationship between endocrine parameters and reproductive activity can offer important insights into how social and environmental factors influence the reproductive success of mammals. Although components of both the physical and social environment affect endocrine regulation of reproduction, less is understood about the potential role of interactions between different endocrine axes on reproductive activity. We evaluated temporal patterns of reproductive and adrenocortical steroids in two male and three female aardwolves (Proteles cristata) housed in captivity at Brookfield Zoo, Chicago, IL, USA. We found seasonal variation in faecal androgens, estrogens, and progestagens, which provide support for previous observations of the aardwolf as a seasonal breeder. However, the timing of peak endocrine activity did not correspond to observations from wild populations. Our interpretation is that this discrepancy is caused by photoperiodic regulation of reproductive activity. We found a positive relationship between faecal androgens and faecal glucocorticoid metabolites in males and a positive relationship between faecal estrogens and faecal glucocorticoid metabolites in females when housed with conspecifics but not when housed alone. We also found a positive but asymptotic relationship between faecal progestagens and faecal glucocorticoid metabolites. We argue that these observations indicate a potential effect of reproductive endocrine activity on the hypothalamic–pituitary–adrenal axis, which could result in interesting physiological trade-offs in male reproductive tactics and female pre-partum maternal investment because of the negative effects of long-term glucocorticoid elevation on reproductive performance. Finally, our results suggest that social and environmental factors interact in regulating many aspects of endocrine fluctuations in this mostly solitary species. 相似文献
15.
Abstract In the terrestrial crustacean Armadillidium vulgare, the prolonged presence of males along with females is known to boost female reproductive physiology, and the so-called ‘male-effect’ was best characterized by a significant shortening of the pre-parturial intermoult (PPI) during which oocyte maturation spontaneously takes place. Continuous presence of a male over that period can speed up vitellogenesis and the moulting cycle, so to reduce female PPI by 15–20 days (shortening of 30–40%, in comparison to females reared with other females or in isolation, respectively). In the last investigation on the subject, we revealed that sexual interactions may start much sooner than previously thought in this species, and suggested that the observed ‘male-effect’ may likely result from early mating stimulations. Here we tested the specific effect of controlled mating interactions (one or two mating events at different times) on female PPI. We revealed that male presence for the time (about 2 h) that allowed a single mating to occur was enough to reduce the female intermoult by about 10 days (shortening of 19%, in comparison to females reared in similar conditions but in the absence of a mating interaction). Moreover, results indicate that the earlier and longer the copulations, the stronger the ‘male-effect’. Altogether, the data support the conclusion that A. vulgare females are adjust their reproductive physiology according to the presence/absence and the intensity of male mating stimuli. We place the findings in a broader ecological context, revise the so far prevailing view on the ‘male-effect’, and stress its possible significance in relation to the occurrence of feminising bacteria in this and other terrestrial isopod species. 相似文献
16.
The morphology of male genitalia whilst stable within species, exhibits huge interspecific variation. This variation is likely
to be as a result of sexual selection due to the direct involvement of these reproductive structures in mating and sperm transfer.
In contrast, internal soft tissue components of the genitalia are generally poorly investigated as they are not directly involved
in physical and mechanical adequacy during sperm transfer. However, these soft tissue structures may also drive differential
male–female interactions, particularly in internally fertilising organisms where females have the ability to store sperm and
bias male reproductive success. In this paper we use the drosophila model to investigate the role of male and female reproductive
elements in sexual selection. Our meta-analysis supplemented with additional new data clearly shows that within species, sperm
length versus testis length, and sperm length versus seminal receptacle length, are highly correlated. Thus, independent of
the phylogenetic relationship among species, gamete evolution is likely to result in sexual selection interactions that drive
the evolution of internal reproductive components in both sexes. Our results and discussion of the literature highlight the
importance of considering internal soft structures that may influence fertilisation, when investigating selective forces acting
on the evolution of reproductive traits. 相似文献
17.
Rick Hochberg 《Zoomorphology》2009,128(2):169-181
The platyhelminth reproductive system is a complex series of canals, glands, and sclerotic components that figure prominently
in our understanding of reproductive physiology, taxonomy, and evolution of the Platyhelminthes. Yet, there is limited information
on its innervation, especially for free-living species of Rhabdocoela, the most speciose and ecologically diverse assemblage.
Here, innervation of the reproductive system in the common marine kalyptorhynch, Gyratrix hermaphroditus, is studied using confocal laser scanning microscopy (CLSM), fluorescent phalloidin, and antibodies to serotonin (5HT) and
small cardioactive peptide b (SCPb). Results show that portions of the female atrial system (uterus, female gonopore) are innervated by peptidergic (SCPb) neurons that may function to control muscles involved in egg movement. In contrast, portions of the male atrial system (male
atrium, male gonopore) are innervated by both peptidergic and serotonergic neurons. These neurons form a complex series of
hoops around the musculature of the male atrium that houses prostate stylet type II. It is hypothesized that 5HT is the primary
myoexcitatory neurotransmitter, and that it acts either synergistically with SCPb to trigger muscle contractions of the male atrium and protract the copulatory stylet, or that SCPb plays an inhibitory role during contraction of the male atrium. A comparison of the distribution of peptidergic and serotonergic
neurons in G. hermaphroditus with other free-living species and parasitic rhabdocoels (Neodermata) reveals similarities in innervation of comparable (though
probably not homologous) reproductive organs. These results suggest that the atrial systems of phylogenetically diverse species
may share a common neuronal physiology despite their structural differences and potential independent evolutionary origins
within the Platyhelminthes. 相似文献
18.
Anita I. Stone 《International journal of primatology》2014,35(3-4):628-642
Sexually dimorphic traits can evolve through male–male competition or female choice. Squirrel monkeys (genus Saimiri) live in large multimale, multifemale groups and are seasonal breeders with concealed ovulation. In several species of the genus, females are dominant to males. Males show weight gain preceding and during the mating season, which produces a “fattened” appearance in the upper arms, shoulders, and torso. Although much is known about the physiology of fattening, the evolutionary function(s) of this sexually dimorphic trait, including possible benefits and costs, remain(s) unknown. This unusual reproductive physiology of males is suggestive of sexual selection. Here I present data on wild Saimiri sciureus studied in Brazil to describe male reproductive investment in the species and to examine the hypothesis that male fattening is a product of sexual selection. I observed at least nine adult males via focal animal sampling and ad libitum observations during four mating seasons and during an additional 10 nonbreeding season months for comparison. Compared to less robust males, fatter males spent significantly more time near females and less time alone. These males also spent more time engaged in sociosexual activities and less time feeding/foraging, suggesting a trade-off between maintenance and reproductive behaviors. The 2-mo mating season accounted for 62% of all male–male agonism observed over one 12-mo period. These results are suggestive of male–male competition for females. However, males did not coerce females to mate and females often rejected males; this pattern suggests female choice. It is possible that male fattening in Saimiri is a product of both intra- and intersexual selection. Males experience intense reproductive costs related to agonism with other males, and related to time and energy invested in the pursuit of females. Although fattening may mitigate some of these costs by aiding in male–male competition and in female preference, this phenomenon is likely not without physiological costs to adult males. 相似文献
19.
Appalachian species of Cryptocercus (Dictyoptera: Cryptocercidae) display considerable genetic variation, but little morphological variation has been reported. We employed light and scanning electron microscopy to investigate variation in male and female reproductive structures among four species of Cryptocercus. Our results indicated consistent, species-specific differences exist in the genitalia of the four species. Males exhibit moderate interspecific differences in the shape of the subgenital plate and third left phallomere hook. Females display consistent interspecific differences in the size of setae on the basivalvula and great differences in size, number, and pattern of setae on the base of the second valves. Together, the interspecific variation in female reproductive structures can be used to identify each of the four Cryptocercus species that occur in the Appalachian Mountains. 相似文献
20.
Rooster infertility is a major concern in the poultry industry and chicken male reproductive organs are the infectious tissues of various pathogenic microorganisms. Protection of the chicken male reproductive organs from pathogens is therefore an essential aspect of reproductive physiology. Recently Toll-like receptors (TLRs) have been identified as one of the key components of innate immunity in vertebrate species and have been reported to be expressed in the reproductive organs in various female species, including the chicken. However, mechanisms of antimicrobial protection of male reproductive organs mediated by TLRs are poorly understood. The objectives of this study were to determine the expression profile of the entire family of the ten chicken TLR genes in the chicken testis, to investigate whether sexual maturation affects their testicular mRNA abundance and to determine the changes in their expression levels in response to Salmonella enteritidis (SE) infection. RNA was extracted from the testis of healthy pre-pubertal, sexually mature and aged birds, and from sexually mature SE infected birds. RT-PCR analysis revealed that all TLRs, apart from TLR1-1 (TLR6), were expressed in the chicken testis. Quantitative real-time PCR analysis revealed that the testicular mRNA abundance of certain TLRs was developmentally regulated with respect to sexual maturation, while SE infection resulted in a significant induction of TLR2-1, 4, 5, 15 and 21 in the testis of sexually mature birds compared, to healthy birds of the same age. These findings provide strong evidence to suggest a key role of TLRs in the innate immune responses of chicken testis against Salmonella colonization. 相似文献