共查询到20条相似文献,搜索用时 15 毫秒
1.
Kazushige Yamana Akira Yoshikawa Rie Noda Hidehiko Nakano 《Nucleosides, nucleotides & nucleic acids》2013,32(1-3):233-242
Abstract Incorporation of an azobenzene-4,4′-diamide group via a linker arm into the 3′-hydroxyl function of one oligonucleotide segment and the 5′-OH of other oligonucleotide has been described. The binding of the oligonucleotides containing the azobenzene linker was investigated by UV melting behaviors. The azobenzene linker has been shown to be useful as an effective bridge for stabilizing hairpin duplex and triplex. 相似文献
2.
Diego A. Gianolio Larry W. McLaughlin 《Nucleosides, nucleotides & nucleic acids》2013,32(8):1751-1769
Abstract Two pyrimidine nucleosides have been synthesized containing extended hydrogen bonding functionality. In one case the side chain is based upon semicarbazide and in the second monoacetylated carbohydrazide was employed. DNA sequences could be prepared using both analogue nucleosides in a reverse coupling protocol, and provided that the normal capping step was eliminated and that the iodine-based oxidizing solution was replaced with one based upon 10-camphorsulfonyl oxaziridine. Both derivatives exhibited moderate effects in targeting selectively C-G base pairs embedded within a polypurine target sequence. 相似文献
3.
Radhakrishnan P. Iyer Dong Yu Theresa Devlin Nan-Hui Ho Suzanne Johnson Sudhir Agrawal 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1491-1495
Abstract Mixed backbone oligonucleotides (MBOs) (containing ionic and non-ionic internucleotidic linkages) in which the non-ionic segments are either methylphosphotriester (PO-OMe) or primary phosphoramidate (PO-NH2) linkages have been prepared using the recently described N-pent-4-enoyl (PNT) nucleoside phosphoramidates and H-phosphonates. Biophysical properties and stability studies suggest that these MBOs are novel antisense molecules. 相似文献
4.
Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces. To fulfil this requirement on lipid membranes we have synthesised a thymidine monomer modified with a cholesterol moiety. Solution studies show that the melting temperature (T m ) of the duplex, with adjacent cholesterols on each strand, is much higher than that of the unmodified duplex. 相似文献
5.
K. Jayaraman R. H. Durland T. S. Rao G. R. Revankar V. Bodepudi N. Chaudhary 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):951-955
Abstract The ability of simple azole nucleosides to promote antiparallel triplex formation at non-homopurine duplex targets was explored. It was also shown that spermidine-cholesterol conjugation is effective in enhancing cellular uptake and stability of oligonucleotides. 相似文献
6.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1117-1118
Abstract The efficient synthesis of oligonucleotides containing 2′-O-β-D-ribofuranosyl (and β-D-ribopyranosyl)nucleosides, 2′-O-α-D-arabinofuranosyl (and α-L-arabinofuranosyl)nucleosides, 2′-O-β-D-erythrofuranosylnucleosides, and 2′-O-(5′-amino-5-deoxy-β-D-ribofuranosyl)nucleosides have been developed. 相似文献
7.
Abstract Oligonucleotides containing novel phoshoramidite 12 were synthesized and studied for their hybridization properties for the first time. Interestingly, these modified oligonucleotides showed a remarkable resistance to exonuclease. 相似文献
8.
Abstract 2,4-Dinitroaniline is an efficient intramolecular fluorescence-quencher for fluorescein - labeled oligonucleotides and interacts with the heterocyclic bases on duplex formation. Consequently, intramolecular fluorescence quenching is disturbed in double labeled oligonucleotides of this type, and fluorescein shows strong fluorescence in a duplex form. There is a substential increase of the fluorescence-quantum yield when the marker and quencher is attached to a single guanosine residue. Two kinds of doubly labeled oligonucleotides have been synthesized, using the NPE/NPEOC strategy. 相似文献
9.
Abstract The properties of the degenerate nucleosides dP and dK, in templates and primers were determined. dP was copied as either pyrimidine, dK as either purine. In primers, an equimolar mixture of the two nucleosides functioned as a universal base equivalent in both sequencing and the polymerase chain reactions. Cloning of DNA containing dP or dK produced transition mutations in vivo. 相似文献
10.
Stuart Dimock Balkrishen Bhat Didier Peoc'h Yogesh S. Sanghvi Eric E. Swayze 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1629-1632
Abstract The facile preparation of two novel classes of nucleoside analogs for the inclusion as dimeric non-phosphorous containing subunits in chimeric backbones has been accomplished. The concise preparation of 3′-formylnucleosides and 5′-O-(N-methylhydroxylamino)-nucleosides is reported. 相似文献
11.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):645-648
Abstract The synthesis of parallel hairpins carrying 8-aminopurines is described. These hairpins have a high affinity for specific polypyrimidine sequences resulting in the formation of very stable triplexes. 相似文献
12.
Zhukov S. A. Pyshnyi D. V. Kupryushkin M. S. 《Russian Journal of Bioorganic Chemistry》2021,47(2):380-389
Russian Journal of Bioorganic Chemistry - Novel representatives of phosphoryl guanidine oligonucleotide derivatives were prepared in this study. A synthetic scheme has been proposed and implemented... 相似文献
13.
Abstract 6-Phenyl, 7-phenyl, 6-(4-biphenyl)-, 7-(4-biphenyl)lumazine N1-(2′-deoxy-D-ribofuranosides) were synthesized and incorporated in the different positions of self-complementary oligodeoxyribonucleotides, and the influence of modifications on the melting points of duplexes was studied. 相似文献
14.
Shigenori Iwai 《Nucleosides, nucleotides & nucleic acids》2013,32(4-6):561-582
Since nucleic acids are organic molecules, even DNA, which carries genetic information, is subjected to various chemical reactions in cells. Alterations of the chemical structure of DNA, which are referred to as DNA damage or DNA lesions, induce mutations in the DNA sequences, which lead to carcinogenesis and cell death, unless they are restored by the repair systems in each organism. Formerly, DNA from bacteria and bacteriophages and DNA fragments treated with UV or γ radiation, alkylating or crosslinking agents, and other carcinogens were used as damaged DNA for biochemical studies. With these materials, however, it is difficult to understand the detailed mechanisms of mutagenesis and DNA repair. Recent progress in the chemical synthesis of oligonucleotides has enabled us to incorporate a specific lesion at a defined position within any sequence context. This method is especially important for studies on mutagenesis and translesion synthesis, which require highly pure templates, and for the structural biology of repair enzymes, which necessitates large amounts of substrate DNA as well as modified substrate analogs. In this review, the various phosphoramidite building blocks for the synthesis of lesion-containing oligodeoxyribonucleotides are described, and some examples of their applications to molecular and structural biology are presented. 相似文献
15.
Mitsuharu Kotera Anne-Gaëlle Bourdat Eric Defrancq Muriel Jourdan Julian Garcia Christian Coulombeau 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1323-1324
Abstract A highly efficient synthetic method for incorporation of the deoxyribonolactone lesion into oligonucleotides was developed using the photochemical properties of 7-nitroindole. 相似文献
16.
Natalia Dyatkina Alexander Khorlin Yuri Khrlin Kyoichi A. Watanabe 《Nucleosides, nucleotides & nucleic acids》2013,32(4-5):621-622
Abstract Phosphoramidite of (1,2,4-triazol-1-yl)-4-[(4-pyren-1-ylbutyl)amino]-5-(2-O-methyl-5-dimethoxytrityl-β-D-ribofuranosyl)pyrimidine has been synthesized, incorporated into polypyrimidine oligoaucleotides, and studied for thier triplex forming capacity. 相似文献
17.
Andrei Guzaev Guity Balow Muthiah Manoharan 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1391-1392
Abstract Novel oligonucleotide analogs that bear phosphodiester and bioreversible S-pivaloyl 2-mercaptoethyl (SPME) phosphate triester internucleosidic linkages and their thioate analogs are described. Their synthesis involves new methodology for the deprotection of base-labile oligonucleotides. 相似文献
18.
T. Sudhakar Rao Michael E. Hogan Ganapathi R. Revankar 《Nucleosides, nucleotides & nucleic acids》2013,32(1-3):95-107
Abstract N7-Benzoyl-2′-deoxyformycin A (5) was prepared from formycin A and incorporated into the triple helix forming oligonucleotide PRE2ap at CG inversion sites. The modified oligonucleotide containing three substitutions of 2′-deoxyformycin A displayed a 10-fold increase in binding affinity as compared to its unmodified counterpart. This provided a method to accommodate CG inversion sites within target sites for antiparallel triple helix formation. 相似文献
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20.
Kanda S. Ramasamy Vesna Stoisavljevic 《Nucleosides, nucleotides & nucleic acids》2013,32(8):1845-1861
Abstract Novel serine derivative of thymine was prepared and incorporated into oligonucleotides. These modified oligonucleotides were studied for their binding affinity with complementary DNA/RNA. 相似文献