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1.
Soybean looper, Pseudoplusia includens (Walker), oviposition in cotton, Gossypium hirsutum L., and soybean, Glycine max (L.) Merr., of various stages of plant phenological development was evaluated in field cages in 1994, 1995, and 1996. Overall, females preferred to oviposit on soybean over cotton when both crops were compared in vegetative or prebloom stages, when both crops were blooming, and when soybean was blooming or in early pod stage compared with prebloom cotton. Females preferred to deposit eggs on the lower leaf surface in the upper two-thirds of the plant canopy in cotton and soybean. Oviposition in upper and middle canopy levels varied with plant growth stage. Females tended to lay more eggs in the upper canopy compared with the middle canopy in prebloom cotton and vegetative soybean; more eggs were laid in the middle canopy of blooming cotton and reproductive stages of soybean. Females responded to both cotton and soybean volatiles in an olfactometer. There was no significant difference in response to the two sources of volatiles. 相似文献
2.
L. M. Mansur K. G. Lark H. Kross A. Oliveira 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(8):907-913
Quantitative trait loci (QTL) were mapped in segregating progeny from a cross between two soybean (Glycine max (L.) Merr.) cultivars: Minsoy (PI 27.890) and Noir 1 (PI 290.136). The 15 traits analyzed included reproductive, morphological, and seed traits, seed yield and carbon isotope discrimination ratios (13C/12C). Genetic variation was detected for all of the traits, and transgressive segregation was a common phenomenon. One hundred and thirty-two linked genetic markers and 24 additional unlinked markers were used to locate QTL by interval mapping and one-way analysis of variance, respectively. Quantitative trait loci controlling 11 of the 15 traits studied were localized to intervals in 6 linkage groups. Quantitative trait loci for developmental and morphological traits (R1, R5, R8, plant height, canopy height, leaf area, etc.) tended to be clustered in three intervals, two of which were also associated with seed yield. Quantitative trait loci for seed oil were separated from all the other QTL. Major QTL for maturity and plant height were linked to RFLP markers R79 (31% variation) and G173 (53% variation). Quantitative trait loci associated with unlinked markers included possible loci for seed protein and weight. Linkage between QTL is discussed in relation to the heritability and genetic correlation of the traits. 相似文献
3.
Identification of quantitative trait loci for plant height, lodging, and maturity in a soybean population segregating for growth habit 总被引:8,自引:0,他引:8
S. H. Lee M. A. Bailey M. A. R. Mian E. R. Shipe D. A. Ashley W. A. Parrott R. S. Hussey H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(5):516-523
The use of molecular markers to identify quantitative trait loci (QTLs) has the potential to enhance the efficiency of trait selection in plant breeding. The purpose of the present study was to identify additional QTLs for plant height, lodging, and maturity in a soybean, Glycine max (L.) Merr., population segregating for growth habit. In this study, 153 restriction fragment length polymorphisms (RFLP) and one morphological marker (Dt1) were used to identify QTLs associated with plant height, lodging, and maturity in 111 F2-derived lines from a cross of PI 97100 and Coker 237. The F2-derived lines and two parents were grown at Athens, Ga., and Blackville, S.C., in 1994 and evaluated for phenotypic traits. The genetic linkage map of these 143 loci covered about 1600 cM and converged into 23 linkage groups. Eleven markers remained unlinked. Using interval-mapping analysis for linked markers and single-factor analysis of variance (ANOVA), loci were tested for association with phenotypic data taken at each location as well as mean values over the two locations. In the combined analysis over locations, the major locus associated with plant height was identified as Dt1 on linkage group (LG) L. The Dt1 locus was also associated with lodging. This locus explained 67.7% of the total variation for plant height, and 56.4% for lodging. In addition, two QTLs for plant height (K007 on LG H and A516b on LG N) and one QTL for lodging (cr517 on LG J) were identified. For maturity, two independent QTLs were identified in intervals between R051 and N100, and between B032 and CpTI, on LG K. These QTLs explained 31.2% and 26.2% of the total variation for maturity, respectively. The same QTLs were identified for all traits at each location. This consistency of QTLs may be related to a few QTLs with large effects conditioning plant height, lodging, and maturity in this population. 相似文献
4.
M. A. R. Mian R. Wells T. E. Carter Jr. D. A. Ashley H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):354-360
Selection for high specific leaf weight (SLW) in soybean [Glycine max (L) Merr.] may increase apparent photosynthetic rate per unit leaf area (AP), which in turn may improve seed yield. In general,
the SLW and leaf size are negatively correlated in soybean. To maximize total photosynthetic performance, and perhaps the
seed yield, of a soybean cultivar, it would be necessary to establish a large leaf area rapidly while maintaining a high SLW.
The objective of the present study was to identify quantitative trait loci (QTLs) conditioning SLW and leaf size in soybean.
One hundred and twenty F4-derived lines from a ‘Young’×PI416937 population were evaluated using restriction fragment length polymorphism (RFLP) markers.
The genetic map consisted of 155 loci on 33 linkage groups (LGs) covering 973 cM of map distance. The phenotypic data were
collected from two different environments – a greenhouse at Athens, Ga. and a field site at Windblow, N.C. The SLW and leaf-size
measurements were made on leaves from the 8th and 9th node of soybean plants at the V12 stage of development. Combined over
environments, six putative independent RFLP markers were associated with SLW, and four of these loci were consistent across
environments. Individually, the six markers each explained between 8 and 18% of the phenotypic variation among lines for SLW.
The Young alleles contributed to a greater SLW at four of the six independent marker loci, and transgressive segregation occurred
among the progeny for SLW. Three putative independent RFLP markers were associated with leaf size, each explaining between
6 to 11% of the phenotypic variation in the trait, and one of these markers was identified in both environments. There was
no correlation between SLW and leaf size in this population. Similarly, none of the six QTLs conditioning SLW were linked
to any of the three QTLs for leaf size. In this soybean population, it is possible to select for progeny lines with greater
SLW than either parent perhaps without affecting the leaf size. It is feasible to pyramid all of the desirable alleles for
greater SLW and large leaf size in a single genetic background.
Received: 16 August 1997 / Accepted: 20 October 1997 相似文献
5.
Dana Holá Olga Rothová Marie Kočová Ladislav Kohout Miroslav Kvasnica 《Plant Growth Regulation》2010,61(1):29-43
The response of two field-grown inbred lines of maize (Zea mays L.) and their F1 hybrid to the application of 10−8–10−14 M solutions of 24-epibrassinolide or synthetic androstane analogue of castasterone in V3/4 and V6/7 developmental stages
was followed during the vegetative and early reproductive phases of plant development. Brassinosteroids (BRs) significantly
affected (either positively or negatively, depending on the genotype and the developmental stage they were applied) the height
of plants during the early weeks after their application, but not the final plant height nor the number of leaves. Spraying
of plants with BRs in V3/4 developmental stage usually also increased the length of the 7th to 10th leaf, whereas the application
in V6/7 developmental stage had the opposite effect. The beginning of the reproductive phase of plant development and the
course of flowering was strongly influenced by the application of BRs. Treatment of plants in V3/4 stage delayed and treatment
of plants in V6/7 stage advanced the dates of anthesis and silking, regardless of the type of BR used, its concentration or
plant genotype. The influence of BRs on the development of the secondary ear was the least pronounced in the F1 hybrid; in
both inbred lines it strongly depended on the concentrations of BRs used. Various yield parameters were also affected by treatment
of plants with BRs, but this effect depended on the developmental stage during which the application of BRs occured, the plant
genotype, the type of BR and its concentration. 相似文献
6.
Effects of weed management systems on canopy insects in herbicide-resistant soybeans 总被引:4,自引:0,他引:4
The effects of transgenic herbicide-resistant soybean varieties and their corresponding weed management strategies on canopy insects were examined in studies at two locations in Iowa in 1997 and 1998. Weed management systems that allowed more weed escapes typically had higher insect population densities. However, systems with fewer weeds seemingly were preferred by potato leafhoppers. Bean leaf beetles and potato leafhoppers showed preferences for certain soybean varieties, but these effects were attributed to soybean plant height. These findings indicate that although the transgenic soybean varieties did not strongly affect insect populations, weed management systems can affect insect populations in soybean. However, this impact is likely related more to weed suppression effectiveness than to a direct effect of the herbicides on the insects. 相似文献
7.
Sun D Li W Zhang Z Chen Q Ning H Qiu L Sun G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):665-673
Quantitative trait loci (QTLs) identified so far in soybean were mainly derived in the final stage of plant development, which
did not apply to the exploitation of genetic effects that were expressed during a specific developmental stage. Thus, the
aim of this study was to identify conditional QTLs associated with yield traits at a specific developmental interval of soybean
plant. The 143 recombinant inbred lines developed from the cross of soybean cultivars ‘Charleston’ and ‘Dongnong 594’ were
used for the developmental QTLs analysis of pod number in the main stem and plant height by composite interval mapping method
combined with mixed genetic model. The results indicated that the number and type of QTLs and their genetic effects for the
two agronomic traits were different in a series of measuring stages. A total of 10 unconditional QTLs in 6 linkage groups
and 5 conditional QTLs in 3 linkage groups were identified for the pod number of the main stem, while 13 unconditional QTLs
in 7 linkage groups and 12 conditional QTLs in 6 linkage groups were identified for plant height. Many QTLs that were detected
in the early stages were different from those detected at the later stages. Some QTLs existed only at one stage and others
existed across two or three stages. Five marker intervals (satt509-satt251, sat_099-sat_113, sat_113-OPAW19_4, satt457-OPC10_85,
sat_095-OPBA08_5) were proven to be associated both with the development of pod number in the main stem and the development
of plant height. The present study suggested that the development of pods and plant height in soybean were governed by time-dependent
gene expression. 相似文献
8.
Delin Li Dong Bai Yu Tian Ying-Hui Li Chaosen Zhao Qi Wang Shiyu Guo Yongzhe Gu Xiaoyan Luan Ruizhen Wang Jinliang Yang Malcolm J. Hawkesford James C. Schnable Xiuliang Jin Li-Juan Qiu 《植物学报(英文版)》2023,65(1):117-132
Advances in plant phenotyping technologies are dramatically reducing the marginal costs of collecting multiple phenotypic measurements across several time points. Yet, most current approaches and best statistical practices implemented to link genetic and phenotypic variation in plants have been developed in an era of single-time-point data. Here, we used time-series phenotypic data collected with an unmanned aircraft system for a large panel of soybean (Glycine max (L.) Merr.) varieties to identify previously uncharacterized loci. Specifically, we focused on the dissection of canopy coverage (CC) variation from this rich data set. We also inferred the speed of canopy closure, an additional dimension of CC, from the time-series data, as it may represent an important trait for weed control. Genome-wide association studies (GWASs) identified 35 loci exhibiting dynamic associations with CC across developmental stages. The time-series data enabled the identification of 10 known flowering time and plant height quantitative trait loci (QTLs) detected in previous studies of adult plants and the identification of novel QTLs influencing CC. These novel QTLs were disproportionately likely to act earlier in development, which may explain why they were missed in previous single-time-point studies. Moreover, this time-series data set contributed to the high accuracy of the GWASs, which we evaluated by permutation tests, as evidenced by the repeated identification of loci across multiple time points. Two novel loci showed evidence of adaptive selection during domestication, with different genotypes/haplotypes favored in different geographic regions. In summary, the time-series data, with soybean CC as an example, improved the accuracy and statistical power to dissect the genetic basis of traits and offered a promising opportunity for crop breeding with quantitative growth curves. 相似文献
9.
R. A. Teutonico T. C. Osborn 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(7-8):885-894
A linkage map of restriction fragment length polymorphisms (RFLPs) was constructed for oilseed, Brassica rapa, using anonymous genomic DNA and cDNA clones from Brassica and cloned genes from the crucifer Arabidopsis thaliana. We also mapped genes controlling the simply inherited traits, yellow seeds, low seed erucic acid, and pubescence. The map included 139 RFLP loci organized into ten linkage groups (LGs) and one small group covering 1785 cM. Each of the three traits mapped to a single locus on three different LGs. Many of the RFLP loci were detected with the same set of probes used to construct maps in the diploid B. oleracea and the amphidiploid B. napus. Comparisons of the linkage arrangements between the diploid species B. rapa and B. oleracea revealed six LGs with at least two loci in common. Nine of the B. rapa LGs had conserved linkage arrangements with B. napus LGs. The majority of loci in common were in the same order among the three species, although the distances between loci were largest on the B. rapa map. We also compared the genome organization between B. rapa and A. thaliana using RFLP loci detected with 12 cloned genes in the two species and found some evidence for a conservation of the linkage arrangements. This B. rapa map will be used to test for associations between segregation of RFLPs, detected by cloned genes of known function, and traits of interest. 相似文献
10.
D. G. Alston J. R. Bradley Jr. D. P. Schmitt H. D. Coble 《Entomologia Experimentalis et Applicata》1991,58(3):279-288
The influence of canopy development in soybean on the survival of corn earworm, Heliothis zea (Boddie) (Lepidoptera: Noctuidae), egg and larval stages and population dynamics of arthropod fauna were evaluated in field trials during 1986–88 in eastern North Carolina. Soybean canopy size decreased as soybean cyst nematode, Heterodera glycines Ichinohe (Nematoda: Heteroderidae), initial population densities increased. Plant species composition of the soybean canopy was affected by weed population densities. Mortality of H. zea larvae due to parasitism and infection with entomopathogens was greater in closed canopy and (or) weedy soybeans than in very open and (or) weed free soybeans. Predation and parasitism of corn earworm eggs were similar across nematode and weed density treatments. Natural enemy populations increased to highest levels during July in closed canopy and (or) weedy soybeans, coinciding with availability of largest prey population reservoirs. A delay in colonization of very open and (or) weed free soybeans by beneficial arthropods until mid to late August allowed greater H. zea larval survival than in closed canopy and (or) weedy soybeans. Arthropod species richness was generally greatest in closed canopy and (or) weedy soybeans during mid to late July, with differences becoming nonsignificant in August and early September. Mean and maximum ambient temperatures were higher and relative humidities lower in open canopy than in closed canopy plots. These conditions were less favorable for development of pathogens and natural enemies. 相似文献
11.
Linkage Groups of Protein-Coding Genes in Western Palearctic Water Frogs Reveal Extensive Evolutionary Conservation 总被引:4,自引:0,他引:4
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Among progeny of a hybrid (Rana shqiperica X R. lessonae) X R. lessonae, 14 of 22 loci form four linkage groups (LGs): (1) mitochondrial aspartate aminotransferase, carbonate dehydratase-2, esterase 4, peptidase D; (2) mannosephosphate isomerase, lactate dehydrogenase-B, sex, hexokinase-1, peptidase B; (3) albumin, fructose-biphosphatase-1, guanine deaminase; (4) mitochondrial superoxide dismutase, cytosolic malic enzyme, xanthine oxidase. Fructose-biphosphate aldolase-2 and cytosolic aspartate aminotransferase possibly form a fifth LG. Mitochondrial aconitate hydratase, α-glucosidase, glyceraldehyde-3-phosphate dehydrogenase, phosphogluconate dehydrogenase, and phosphoglucomutase-2 are unlinked to other loci. All testable linkages (among eight loci of LGs 1, 2, 3, and 4) are shared with eastern Palearctic water frogs. Including published data, 44 protein loci can be assigned to 10 of the 13 chromosomes in Holarctic Rana. Of testable pairs among 18 protein loci, agreement between Palearctic and Nearctic Rana is complete (125 unlinked, 14 linked pairs among 14 loci of five syntenies), and Holarctic Rana and Xenopus laevis are highly concordant (125 shared nonlinkages, 13 shared linkages, three differences). Several Rana syntenies occur in mammals and fish. Many syntenies apparently have persisted for 60-140 X 10(6) years (frogs), some even for 350-400 X 10(6) years (mammals and teleosts). 相似文献
12.
Eugenia Martin Vanina Cravero Ezio Portis Davide Scaglione Esteban Acquaviva Enrique Cointry 《Molecular breeding : new strategies in plant improvement》2013,32(1):177-187
An F1 mapping population was bred by crossing an accession of wild cardoon with a single Argentinian globe artichoke plant of the variety Estrella del Sur FCA with a view to generating new Cynara cardunculus linkage maps. Genotyping was conducting using a set of 553 SRAP, SSR, AFLP and SNP markers. The 1,465.5 cM map based on the segregation of alleles present in the wild cardoon parent comprised 214 loci distributed across 16 linkage groups (LGs), while the 910.1 cM globe artichoke-based map featured 141 loci falling into 12 LGs covering the total length. Three of the morphological traits (head spininess, leaf spininess and head color) for which the parents contrasted were inherited monogenically, and the genes conditioning them were mapped. A set of 48 co-dominant loci was used to align the LGs with those derived from a reference SSR-based consensus map of the species. 相似文献
13.
Hisano Hiroshi; Sato Shusei; Isobe Sachiko; Sasamoto Shigemi; Wada Tsuyuko; Matsuno Ai; Fujishiro Tsunakazu; Yamada Manabu; Nakayama Shinobu; Nakamura Yasukazu; Watanabe Satoshi; Harada Kyuya; Tabata Satoshi 《DNA research》2007,14(6):271-281
We generated a high-density genetic linkage map of soybean usingexpressed sequence tag (EST)-derived microsatellite markers.A total of 6920 primer pairs (10.9%) were designed to amplifysimple sequence repeats (SSRs) from 63 676 publicly availablenon-redundant soybean ESTs. The polymorphism of two parent plants,the Japanese cultivar Misuzudaizu and the Chineseline Moshidou Gong 503, were examined using 10%polyacrylamide gel electrophoresis. Primer pairs showing polymorphismwere then used for genotyping 94 recombinant inbred lines (RILs)derived from a cross between the parents. In addition to previouslyreported markers, 680 EST-derived microsatellite markers wereselected and subjected to linkage analysis. As a result, 935marker loci were mapped successfully onto 20 linkage groups,which totaled 2700.3 cM in length; 693 loci were detected usingthe 668 EST-derived microsatellite markers developed in thisstudy, the other 242 loci were detected with 105 RFLP markers,136 genome-derived microsatellite markers, and one phenotypicmarker. We examined allelic variation among 23 soybean cultivars/linesand a wild soybean line using 668 mapped EST-derived microsatellitemarkers (corresponding to 686 marker loci), in order to determinethe transferability of the markers among soybean germplasms.A limited degree of macrosynteny was observed at the segmentallevel between the genomes of soybean and the model legume Lotusjaponicus, which suggests that considerable genome shufflingoccurred after separation of the species and during establishmentof the paleopolyploid soybean genome. 相似文献
14.
15.
Analysis of soybean phenotypes is a core motivation behind soybean breeding. However, amounts of manual measures are required in obtaining canopy phenotypic traits via traditional methods. Moreover, deficiencies such as time consumption, strong subjectivity, and inaccuracy can be also detected in manual measurement. In order to achieve automatic extraction of phenotypic traits in the research of soybean breeding, a method of acquiring soybean phenotypic traits was proposed on the basis of a Kinect sensor with three soybean varieties (incl. KANGXIAN9, KANGXIAN13, and FUDOU6) as research objects, implementing the calculation of plant height (PH), leaf area index (LAI). Firstly, the canopy image information was acquired vertically to extract canopy data with the registration of color images and depth point cloud data. Secondly, a soybean single plant was segmented from the group canopy using the bounding box method; also, the height of the soybean plant was solved using the distance information; meanwhile, the canopy LAI was calculated with extinction coefficients that were optimized by the beer-lambert law. According to experimental results, determination coefficients R2 of the calculated value and the measured value of the plant height and the leaf area index of the three soybean varieties are greater than 0.94. It can be seen that calculated results can meet the accuracy requirement of phenotypic traits in soybean breeding. 相似文献
16.
- The experiment was conducted in the 2016/17 crop season in a greenhouse at Passo Fundo University, Brazil. We hypothesised that the morphological characteristics and biochemical and anatomical composition of soybean roots and shoots, when competing with weeds during different growth periods, are negatively affected, so current concepts of competition between plants should also consider changes in plant roots.
- The soybean cultivar P 95R51 and horseweed (Conyza bonariensis) were used. The treatments consisted of the presence or absence of weeds during different coexistence periods of soybean with horseweed. The periods were V0–V3, V0–V6, V0–R2, V3–R6, V6–R6 and R2–R6, where V0 was the date of soybean sowing and V3, V6, R2 and R6 were phenological stages of the crop. Two fresh roots were used to examine morphological traits. Four roots were used for quantification of dry matter and secondary metabolites.
- Root length was reduced by 21%, 14% and 20% when competing with a weed in the V0–V3, V0–V6 and R2–R6 coexistence periods, respectively. Total phenol content in the V0–V6 and V0–R2 periods was reduced when plants were in competition with weeds; a similar trend was found for flavonoids in the V0–V6 period.
- Soybean–horseweed competition from crop emergence to the V6 stage, in general, affects shoot and root morphological traits and the biochemical composition of the soybean roots. The presence of horseweed at the V3, V6 and R2 stages does not negatively alter the traits evaluated. Root anatomical composition is not modified during all coexistence periods with horseweed.
17.
A low-density genetic map of onion reveals a role for tandem duplication in the evolution of an extremely large diploid genome 总被引:8,自引:0,他引:8
J. J. King J. M. Bradeen O. Bark J. A. McCallum M. J. Havey 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):52-62
The bulb onion, Allium cepa L., is a diploid (2n=2x=16) plant with a huge nuclear genome. Previous genetic and cytogenetic analyses have not supported
a polyploid origin for onion. We developed a low-density genetic map of morphological markers, randomly amplified polymorphic
DNAs (RAPD), and restriction fragment length polymorphisms (RFLP) as a tool for onion improvement and to study the genome
organization of onion. A mapping population of 58 F3 families was produced from a single F1 plant from the cross of two partially inbred lines (Brigham Yellow Globe 15-23 and Alisa Craig 43). Segregations were established
for restoration of male fertility in sterile cytoplasm, complementary light-red bulb color, 14 RAPDs, 110 RFLPs revealed by
90 anonymous cDNA clones, and 2 RFLPs revealed by a cDNA clone of alliinase, the enzyme responsible for the characteristic
Allium flavors. Duplicated RFLP loci were detected by 21% of the clones, of which 53% were unlinked (>30 cM), 5% loosely linked
(10–30 cM), and 42% tightly linked (<10 cM). This duplication frequency is less than that reported for paleopolyploids but
higher than for diploid species. We observed 40% dominant RFLPs, the highest yet reported among plants. Among duplicated RFLP
loci, 19% segregated as two loci each with two codominant alleles, 52% segregated as one locus with codominant alleles and
one locus with only a dominant fragment, and 29% segregated as two loci with only dominant fragments. We sequenced cDNAs detecting
duplicated RFLPs; 63% showed homology to known gene families (e.g., chlorophyll binding proteins, ubiquitin, or RuBISCO),
and 37% were unique clones showing significant homology to known genes of low-copy number or no homology to database sequences.
Duplicated RFLPs showing linkage could be due to retroviral-like sequences in adjacent coding regions or intrachromosomal,
as opposed to whole genome, duplications. Previous cytological analyses and this genetic map support intrachromosomal duplication
as a mechanism contributing to the huge onion genome.
Received: 3 July 1997 / Accepted: 8 August 1997 相似文献
18.
S. Trachsel R. Messmer P. Stamp N. Ruta A. Hund 《Molecular breeding : new strategies in plant improvement》2010,25(1):91-103
A strong photosynthetic performance and rapid leaf development, are important indicators of vigorous early growth. The aim
of this study was to (1) evaluate the tropical maize (Zea mays L.) inbred lines CML444 and SC-Malawi for their photosynthetic performance at different growth stages and (2) assess quantitative
trait loci (QTL) of photosynthesis-related traits in their 236 recombinant inbred lines at the heterotrophic growth stage.
CML444 had a higher leaf chlorophyll (SPAD) content than SC-Malawi. Ten QTLs were found for the quantum efficiency of photosystem
II (ΦPSII; four), SPAD (three) and the specific leaf area (SLA; three). The relevance of seedling QTLs for ΦPSII, SPAD and SLA for yield formation is emphasized by seven collocations (bins 5.01, 7.03, 8.05) with QTLs for kernel number
and grain yield under field conditions. QTLs for SPAD at the V2 and at the reproductive stage did not collocate, indicating
differences in the genetic control of SPAD at different growth stages. Knowing which loci affect SLA, SPAD and ΦPSII simultaneously and which do not will help to optimize light harvest by the canopy. 相似文献
19.
大豆不同生育期根际土壤细菌群落结构的变化 总被引:2,自引:0,他引:2
为了解大豆根际细菌群落结构多样性及根际细菌群落结构的变化,该研究以大豆苗期和成熟期的根际土壤为材料,采用Illumina高通量测序技术测定细菌16S rRNA V3+V4区序列,探究大豆不同生育期根际土壤细菌群落结构的变化。对原始数据进行拼接、过滤、去除嵌合体序列和聚类分析等数据处理,并对OTU进行分类学注释。在此基础上运用ANOVA分析物种组成变化,Alpha多样性指数研究细菌多样性变化。结果表明:细菌丰富度和多样性在不同生育期有显著变化,其中成熟期土壤中的细菌丰富度和多样性指数均明显高于苗期; 变形菌、放线菌、酸杆菌是大豆根际的优势菌门,其含量在不同生育期也有显著变化; 假诺卡氏菌属、糖丝菌属、鞘氨醇单胞菌属是大豆根际的优势菌属,这些菌属中的部分菌群属于根际促生菌,具有潜在的促生效应。这些结果证实大豆的生育期对根际土壤细菌群落结构有重要影响。 相似文献
20.
M. E. Devey T. A. Fiddler B. H. Liu S. J. Knapp D. B. Neale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(3-4):273-278
A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine. 相似文献