CHOP is a multifunctional transcription factor in the ER stress response

The accumulation of unfolded proteins in the endoplasmic reticulum (ER) induces ER stress. To restore ER homeostasis, cells possess a highly specific ER quality-control system called the unfold protein response (UPR). In the case of prolonged ER stress or UPR malfunction, apoptosis signalling is activated. This ER stress-induced apoptosis has been implicated in the pathogenesis of several conformational diseases. CCAAT-enhancer-binding protein homologous protein (CHOP) is induced by ER stress and mediates apoptosis. Recent studies by the Gotoh group have shown that the CHOP pathway is also involved in ER stress-induced cytokine production in macrophages. The multifunctional roles of CHOP in the ER stress response are discussed below.     

Systemic analysis of the response of Aspergillus niger to ambient pH

Background  

The filamentous fungus Aspergillus niger is an exceptionally efficient producer of organic acids, which is one of the reasons for its relevance to industrial processes and commercial importance. While it is known that the mechanisms regulating this production are tied to the levels of ambient pH, the reasons and mechanisms for this are poorly understood.     

Adaptive response to oxidative stress in the filamentous fungus Aspergillus niger B1-D

In the present study, we used a recombinant filamentous fungus strain, Aspergillus niger B1-D, as a model system, and investigated the antioxidant defences in this organism. Our findings indicate that pretreatment with low concentrations of H(2)O(2) completely prevents killing by this oxidant at high concentrations. It shows that A. niger adapts to exposure to H(2)O(2) by reducing growth and inducing a number of antioxidant enzyme activities, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, of which the induction of catalase is the most pronounced. Moreover the decline of these antioxidant enzymes activities after H(2)O(2) detoxification, coincides with recommencement of growth. Results from monitoring the extracellular H(2)O(2) concentration clearly indicate a very rapid detoxification rate for H(2)O(2) in adapted A. niger cultures. A mathematical model predicts only very low concentrations of intracellular H(2)O(2) accumulating in such cultures. Our results also show that glutathione plays a role in the oxidative defence against H(2)O(2) in A. niger. On addition of H(2)O(2), the intracellular pool of glutathione increases while the redox state of glutathione becomes more oxidized.     

The role of ABF family bZIP class transcription factors in stress response

Adverse environmental conditions, such as drought, high salinity and chilling/freezing, are major limiting factors of crop productivity worldwide. According to Boyer [Science 1982;218:443–448], approximately 70% of the genetic potential yield of major crops is lost by these environmental factors. Thus, understanding how plants respond to the 'abiotic stresses' is of agronomic importance. Being sessile, plants have the capability to perceive and respond adaptively to various abiotic stresses. The adaptive capability of plants is largely dependent on the plant hormone abscisic acid (ABA), which regulates, among others, the expression of numerous genes involved in stress adaptation. Extensive genetic and molecular studies have been carried out during the last decade or so, and, as a result, a large body of information is available on the regulatory components of ABA-responsive gene expression under stress conditions. In this article, recent advances in the area are summarized, focusing on the regulatory components of ABA-dependent stress-responsive gene expression.