山葵未成熟胚离体培养和植株再生

1　植物名称　山葵 (Ｅｕｔｒｅｍａｗａｓａｂｉ)日本品种岛根 3号。2　材料类别　开花 2 0ｄ后的荚果中剥出的未成熟种子 (种皮 未成熟胚 )。3　培养条件　基本培养基为自行设计的“贵山”(代号ＧＳ)配方[1 ] 。 ( 1 )诱导愈伤组织培养基为 :ＧＳ 6 ＢＡ 0 .1ｍｇ·Ｌ- 1 (单位下同 ) 2 ,4 Ｄ 1 ;( 2 )芽分化培养基为 :ＧＳ 6 ＢＡ 0 .5 ＺＴ 0 .5 ＩＢＡ0 .0 1 ;( 3)芽增殖培养基为 :ＧＳ 6 ＢＡ 0 .4;( 4 )生根培养基为 :ＧＳ ＩＢＡ 0 .1 ＮＡＡ 0 .1。以上培养基均含 3%蔗糖 [( 1 )含 2 % ]和 0 .6%琼脂粉 ,ｐＨ5 .8～ 5…     

地涌金莲吸芽的离体培养和植株再生

1　植物名称　地涌金莲 (Ｍｕｓｅｌｌａｌａｓｉｏｃａｒｐａ) ,别名地莲花 ,地芭蕉。2　材料类别　吸芽。3　培养条件　以改良的ＭＳ为基本培养基 ,并添加 1 0 %的椰子汁。诱芽培养基 :( 1 )ＢＡ 1 .0ｍｇ·Ｌ- 1 (单位下同 ) ＮＡＡ 0 .1 ;( 2 ) 6 ＢＡ 3.0 ＮＡＡ0 .1 ;(     

山葵的离体培养和植株再生

CInvitroCultureandPlangtletRegenerationofEutremawasabiWANGGuang-Dong,LIShi-Jun(DepartmentofHorticulture,NanjingAgriculturalUniversity,Nanjing210095)1植物名称山葵（Eutremawasabi）,又名山嵛菜。2材料类别带侧芽的根状茎。3培养条件（1）诱导培养基：1／2MS＋6－BA0．2mp·L－1（单位下同）＋NAA0．05;（2）增殖培养基：MS＋6-BA0．2＋KT0．2＋NAA0．05;（3）生根培养基：1／2MS＋NAA0．05。培养基（1）附加琼脂粉5．0g·L－1,其余附加6.0g·L－1。（1）和（2）培养基加蔗糖30g·L－1,（3）加20g·L－…     

山苍子的离体培养和植株再生

1　植物名称　山苍子 (Ｌｉｔｓｅａｃｕｂｅｂａ) ,又名木姜子。2　材料类别　成年植株带芽茎段。3　培养条件　所使用的培养基为 :( 1 )ＭＳ 6 ＢＡ3.0ｍｇ·Ｌ- 1 (单位下同 ) ＩＢＡ 0 .5 ;( 2 )ＭＳ 6 ＢＡ2 .0 ＩＢＡ 0 .5 ;( 3)ＭＳ 6 ＢＡ 1 .0 ＫＴ 1 .0 Ｎ     

瓠瓜的离体培养和植株的高效再生

９￣１０ｄ苗龄的瓠瓜无菌苗的带叶基（子叶片与子叶柄之间的部分）子叶在８种分化培养基上培养１５ｄ左右,结果在含３．５ｍｇ／Ｌ ＺＴ的培养基上能以较高的频率直接分化出芽;分化培养前对带叶基子叶及仅含子叶叶基的两种外植体纵向切割都能明显提高出芽率,不含子叶叶基的外植体不能分化出芽,决定出芽的外植体的部位是子叶叶基;纵向切割子叶叶基前预培养９ｄ以上出芽率大幅度提高。     

枣叶片离体培养再生植株

PlantletRegenerationfromLeavesCulturesofZizyphusiuiubaCHENZong－Li,YANZhi－Lian,QILong（Denyt17mllofmp,You’onl／nll＊,ndy,Yan’as716000）1植物名称枣（凯W…。Wwi）。2材料类别俗名“狗头枣”的无菌试管苗的叶片。3培养条件（l）叶片愈伤组织诱导及继代培养基：MS＋6－BA0．3mg／L（单位下同）＋2,4D20;（2）芽分化培养基：MS＋6－BAI．0＋IBA0．2＋D一泛酸钙1．0十活性发0．5％;（3）芽生长培养基：1／2MS＋6－BA0．2＋IAA0．04＋D一泛酸钙1．0;（4）芽增殖培养基：1／2MS＋6－BA0．4＋IAA0．0…     

厚皮甜瓜的离体培养植株再生

1　植物名称　厚皮甜瓜 (Ｃｕｃｕｍｉｓｍｅｌｏｓｓｐ .Ｐａｎｇ)主栽品种“皇后”。2　材料类别　种子。3　培养条件　诱导胚性愈伤组织培养基 :( 1 )ＭＳ大量及微量元素 (下同 ) 6 ＢＡ 1 .5ｍｇ·Ｌ- 1 (单位下同 ) ;( 2 )ＭＳ Ｂ5 有机 6 ＢＡ 1 ;( 3)ＭＳ ＮＡＡ1 6 ＢＡ 1 ;( 4 )ＭＳ ＮＡＡ 1 6 ＢＡ 0 .5 ;( 5 )ＭＳ ＮＡＡ 1 6 ＢＡ 0 .1 ;( 6)ＭＳ ＩＡＡ 0 .1 6 ＢＡ 1。芽分化培养基 :( 7)ＭＳ 6 ＢＡ 0 .2 ;( 8)ＭＳ 6 ＢＡ0 .5 ;( 9)ＭＳ 6 ＢＡ 1 .0 ;( 1 0 )改良ＭＳ 6 ＢＡ 0 .2 ;( 1 1 )改良…     

红千层离体培养和植株再生

1植物名称红千层(Callistemon rigidus). 2材料类别带芽茎段. 3培养条件(1)初代培养基:MS 6-BA 2.0mg·L-1(单位下同) NAA 0.01 3%蔗糖.(2)增殖培养基:MS 6-BA 1.0 NAA 0.1 3%蔗糖.     

党参的离体培养及植株再生的研究

在附加激素的MS培养基上,培养党参下胚轴和无菌芽切段,诱导产生愈伤组织并且再生植株。经过两年多(15个世代)的继代培养,建立了党参体细胞无性系。实验结果表明:(1)培养基MS 0.4mg/L 2,4-D 0.8mg/L Kt 2.0mg/L IAA对愈伤组织诱导及继代培养,MS 0.2mg/L 6-BA诱导外植体产生丛芽和愈伤组织再分化,MS 0.5mg/L NAA 0.2mg/L 6-BA及MS 0.2mg/L NAA诱导生根效果最好。(2)愈伤组织再分化经过胚状体途径。     

北美枫香的离体培养和植株再生

1植物名称北美枫香(Liquidambar styraciflua L.). 2材料类别幼嫩枝条. 3培养条件(1)腋芽诱导培养基:MS 6-BA 0.2mg·L-1(单位下同);(2)不定芽增殖培养基:MS 6-BA 0.2,0.5,1.0,2.0 NAA 0.2;(3)壮苗培养基:MS 6-BA 0.2 NAA 0.02;(4)生根培养基:1/2MS IBA 0.2 NAA 0.2.以上培养基pH为5.7～5.8,加入3%蔗糖和0.48%琼脂.培养温度为25℃,光照时间为12 h·d-1,光照度为2 000 1x.     

In vitro Culture of Abscissed Immature Avocado Embryos

The efficiency of an avocado hybridization programme, usingsmall potted glasshouse plants, was reduced by a high rate ofabscission of immature fruitlets bearing embryos too young forconventional germination. This was overcome in part by culturein vitro of the shed embryos on a liquid medium supplementedwith 0.5 mg l^–1 benzyladenine. Most embryos younger than6 weeks did not survive in culture, but older embryos slowlyproduced multiple shoots, with axillary shoot growth being furtherstimulated by removal of both cotyledons. Embryo response wasnot related to cultivar. Shoots removed from culture could begrafted to seedling rootstocks. Grafting was considered morereliable than dependence on growth of the main root or adventitiousroots in vitro to produce established plants. Persea americana Miller, avocado, abscissed fruitlets, in vitro embryo culture     

In vitro Culture of Immature Embryos of Cytisus laburnum

Immature embryos of Cytisus laburnum L. were cultivated in vitro and four culture media, different techniques of substrate preparation, sucrose concentration and the effect of suspensor removal were tested. The best results were obtained with N6 medium supplemented with 2 mg dm⁻³ glycine and set up using a double-layer culture system, in which the top layer had a higher osmotic potential than the bottom one. These conditions allowed normal embryogenic development in up to 45 % of early globular embryos, that were able to develop until a complete maturity. Osmotic potential and mineral nutrients of the medium demonstrated to be crucial for the successful culture and their effects were dependent on embryo age at the time of excision. The presence of an intact suspensor showed to be beneficial only for early globular embryos while older developmental stage embryos were not significantly affected. This revised version was published online in July 2006 with corrections to the Cover Date.     

蝴蝶兰EMS离体化学诱变及再生植株RAPD检测

用EMS对离体培养的蝴蝶兰类原球茎薄切片进行化学诱变,研究了不同浓度、不同时间诱变处理对类原球茎薄切片生长、类原球茎再生及再生苗生长的影响,并对再生苗DNA进行了RAPD检测。结果表明,诱变剂EMS对类原球茎薄切片生长产生重要影响,部分薄切片褐化死亡,再生类原球茎生长受到抑制,再生苗数量减少,表现出明显的伤害作用,这种伤害作用随诱变剂浓度的加大和处理时间的延长而加重。诱变剂EMS使部分再生苗畸变,叶片皱缩、变厚。并产生2株叶形、叶色突变体。部分再生苗表现出RAPD图谱带型的多态性,暗示DNA序列发生了一定的变异。EMS适合诱变剂量为浓度0．4％处理2d～4d。     

大豆茎尖离体培养再生植株

1　植物名称　大豆 (Ｇｌｙｃｉｎｅｍａｘ)品种中黄 4号、早熟 1 8和中品 661。2　材料类别　茎尖。3　培养条件　生芽培养基 :( 1 )ＭＳ 6 ＢＡ 3ｍｇ·Ｌ- 1 (单位下同 )。不定芽伸长培养基 :( 2 ) 1 /2ＭＳ 6 ＢＡ 0 .1 ;( 3)ＭＳ ＧＡ30 .5。生根培养基 :( 4 ) 1 /2ＭＳ ＮＡＡ 0 .1。以上培养基均含蔗糖 3%、琼脂0 .8% ,ｐＨ 5 .8;培养室温度 ( 2 6± 2 )℃ ;光照时间每天 1 6ｈ ,光照度 1 60 0～ 2 0 0 0ｌｘ。4　生长与分化情况4.1　靶组织区的制备　取大豆种子 ,以 70 %酒精处理 1ｍｉｎ ,1 %次氯酸钠消毒 1 5ｍｉｎ后 ,用无…     

Development In vitro of White Ash Buds

A system has been established in which the early developmentof freshly inoculated excised buds of Fraxinus americana L.can be obtained in vitro. The structural integrity of the materialwas documented by an anatomical examination of longitudinalstained sections of cultured buds. Fraxinus americana L, white ash, bud culture, growth     

三尖杉的离体胚培养

1植物名称三尖杉(Cephalotaxus fortunei Hook.f.)。2材料类别离体胚。3培养条件芽诱导培养基:MS 6-BA2.0mg·L-1(单位下同) IAA1.0 NAA0.1;芽增殖培养基:MS 6-BA2.0 IAA1.0 NAA1.0;生根培养基:1/2MS IBA1.0 NAA0.5 KT0.2。以上各培养基     

Germplasm Preservation of Wild Arachis Species through Culture of Shoot Apices and Axillary Buds from In Vitro Plants

A study was conducted to evaluate in vitro techniques for germplasm preservation of wild species of Arachis. Nodal segments excised from in vitro-grown plants of A. retusa, A. macedoi and A. burchellii were used to examine the effects of explant position and age of the donor plant. Explants were excised from plants maintained in culture for 30, 60, 90 or 180 d, numbered I – V from top to bottom and cultured on MS medium supplemented with 2.7 µM NAA or different BAP concentrations (0, 4.4, 13.2 and 22 µM). The age of the donor plant has not influenced the responses of the four genotypes studied. In contrast, shoot regeneration ability was significantly affected by the original explant position, decreasing from top to bottom. In media supplemented with different BAP concentrations, multishoot formation was induced from apical segments at low frequencies (10 – 20%) and segments of all positions originated calluses at the explant basis after 30 d of culture. The culture of nodal segments in the presence of 2.7 µM NAA as the sole growth regulator is recommended for the multiplication of in vitro collections of wild groundnut species in order to avoid callusing and adventitious shoot formation.     

In vitro Culture of Immature Cotyledons of Soya Bean (Glycine max L. Merr.)

An in vitro procedure promoting the rapid growth and proteinincrease of soya bean cotyledons has been developed. The amountof protein synthesized varied greatly depending on the nitrogen(N) source provided. Glutamine was the most effective N source,while inorganic forms of N were ineffective. Growth and proteinsynthesis were both more rapid in vitro than in vivo. Underthe best conditions, soya bean cotyledons increased 8-fold bothin dry weight and in protein in 6 days. The formation of the7S and 11S storage proteins in vitro was similar to that invivo. Hence, this in vitro culture method is appropriate forstudying legume seed storage protein synthesis under controlledconditions.