共查询到20条相似文献,搜索用时 31 毫秒
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The central role that different phospholipases play in many signal transduction pathways has been intensively studied by classical biochemical and molecular approaches. One approach not extensively pursued, has been the use of yeast as a model system for functional analysis of different aspects of phospholipase signalling. This review concentrates on attempts to establish yeast in this role and aims to demonstrate the potential offered by this approach. 相似文献
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Donna J. Schlemm Maria J. Crowe Redmond B. McNeill Anne E. Stanley Stephen J. Keller 《Cell stress & chaperones》1999,4(3):171-176
Alcoholic extracts of bakers' yeast (Saccharomyces cerevisiae) have been used for over 60 years in over-the-counter medications for the treatment of hemorrhoids, burns, and wounds. Although previous studies suggested that small peptides were responsible for the medical observations, the peptides were never resolved into separate fractions and identified. In the present report, a protein fraction was prepared by RPC18 chromatography of the extract which enhances wound closure in both diabetic and non-diabetic littermates. The peptides are active in nanomolar amounts and are 600 times more active than the initial extract. SDS-PAGE and N-terminal amino acid sequencing identified 4 polypeptides in the extract. Three of the proteins were small molecular weight stress-associated proteins: copper, zinc superoxide-dismutase, ubiquitin, and glucose lipid regulated protein (HSP 12). The fourth protein, acyl-CoA binding protein II, has not been previously associated with stress proteins. 相似文献
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Chakraburtty K 《The international journal of biochemistry & cell biology》1999,31(1):163-173
Elongation factor 3 (EF-3) is a unique and essential requirement of the fungal translational apparatus. EF-3 is a monomeric protein with a molecular mass of 116,000. EF-3 is required by yeast ribosomes for in vitro translation and for in vivo growth. The protein stimulates the binding of EF-1 alpha :GTP:aa-tRNA ternary complex to the ribosomal A-site by facilitating release of deacylated-tRNA from the E-site. The reaction requires ATP hydrolysis. EF-3 contains two ATP-binding sequence motifs (NBS). NBSI is sufficient for the intrinsic ATPase function. NBSII is essential for ribosome-stimulated activity. By limited proteolysis, EF-3 was divided into two distinct functional domains. The N-terminal domain lacking the highly charged lysine blocks failed to bind ribosomes and was inactive in the ribosome-stimulated ATPase activity. The C-terminally derived lysine-rich fragment showed strong binding to yeast ribosomes. The purported S5 homology region of EF-3 at the N-terminal end has been reported to interact with 18S ribosomal RNA. We postulate that EF-3 contacts rRNA and/or protein(s) through the C-terminal end. Removal of these residues severely weakens its interaction mediated possibly through the N-terminal domain of the protein. 相似文献
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Spin-labeled yeast cells 总被引:1,自引:0,他引:1
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Beta-glucanase of yeast 总被引:6,自引:0,他引:6
T D Brock 《Biochemical and biophysical research communications》1965,19(5):623-629
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A Misaki 《Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme》1970,15(14):1463-1471
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Clasquin MF Melamud E Singer A Gooding JR Xu X Dong A Cui H Campagna SR Savchenko A Yakunin AF Rabinowitz JD Caudy AA 《Cell》2011,145(6):969-980
Glucose is catabolized in yeast via two fundamental routes, glycolysis and the oxidative pentose phosphate pathway, which produces NADPH and the essential nucleotide component ribose-5-phosphate. Here, we describe riboneogenesis, a thermodynamically driven pathway that converts glycolytic intermediates into ribose-5-phosphate without production of NADPH. Riboneogenesis begins with synthesis, by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar sedoheptulose-1,7-bisphosphate. In the pathway's committed step, sedoheptulose bisphosphate is hydrolyzed to sedoheptulose-7-phosphate by the enzyme sedoheptulose-1,7-bisphosphatase (SHB17), whose activity we identified based on metabolomic analysis of the corresponding knockout strain. The crystal structure of Shb17 in complex with sedoheptulose-1,7-bisphosphate reveals that the substrate binds in the closed furan form in the active site. Sedoheptulose-7-phosphate is ultimately converted by known enzymes of the nonoxidative pentose phosphate pathway to ribose-5-phosphate. Flux through SHB17 increases when ribose demand is high relative to demand for NADPH, including during ribosome biogenesis in metabolically synchronized yeast cells. 相似文献
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Huang S 《PLoS biology》2012,10(2):e1001274
This study demonstrates that normal yeast cells can be magnetized, and identifies local redox control via carbon metabolism and iron supply as key factors involved in magnetization. 相似文献
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Tobias Eisenberg Didac Carmona-Gutierrez Sabrina Büttner Nektarios Tavernarakis Frank Madeo 《Apoptosis : an international journal on programmed cell death》2010,15(3):257-268
Necrosis was long regarded as an accidental cell death process resulting from overwhelming cellular injury such as chemical
or physical disruption of the plasma membrane. Such a definition, however, proved to be inapplicable to many necrotic scenarios.
The discovery that genetic manipulation of several proteins either protected or enhanced necrotic cell death argued in favor
of a regulated and hence programmed process, as it is the case for apoptosis. For more than a decade, yeast has served as
a model for apoptosis research; recently, evidence accumulated that it also harbors a necrotic program. Here, we summarize
the current knowledge about factors that control necrotic cell death in yeast. Mitochondria, aging and a low pH are positive
regulators of this process while cellular polyamines (e.g. spermidine) and endonuclease G as well as homeostatic organelles
like the vacuole or peroxisomes are potent inhibitors of necrosis. Physiological necrosis may stimulate intercellular signaling
via the release of necrotic factors that promote viability of healthy cells and, thus, assure survival of the clone. Together,
the data obtained in yeast argue for the existence of a necrotic program, which controls longevity and whose physiological
function may thus be aging. 相似文献
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Summary 1. Twenty-three mutations, mapping at eight different loci, were shown to prevent detectable suppression of ade2-1 and can1-100 by the ochre suppressor, ocSUPQ2. 2. Some reduce the efficiency of suppression of his5-2 and lys1-1 also. 3. Mutants from each of the eight loci prevented the lethal phenotype of ocSUPQ2 in [psi+] strains. They are, therefore, antisuppressor mutations. 4. One of the loci, asu3, is centromere-linked; two of the loci, asu2 and asu6, are linked to each other. 相似文献
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