Sesquiterpene coumarins from the roots of Ferula assa-foetida.

From the chloroform extract of the roots of Ferula assa-foetida, two sesquiterpene coumarins designated assafoetidnol A and assafoetidnol B were isolated, in addition to six known compounds, gummosin, polyanthin, badrakemin, neveskone, samarcandin and galbanic acid. The structures of the sesquiterpenes new compounds were established by spectroscopic methods.     

Terpenoids from Achillea distans Waldst. & Kit. ex Willed

The flower heads of Achillea distans afforded nine new sesquiterpene lactones (one glaucolide, six guaianolides and two dimeric lactones) in addition to 18 known terpenoids. The structures and stereochemistry of the new compounds were elucidated by spectroscopic methods. The stereochemistry of 11,13-dihydroezomontanin was revised.     

Phenylethanoid and lignan glycosides from Verbascum thapsus.

Verbascum thapsus afforded, in addition to three known phenylethanoid glycosides and four lignan ones, five new phenylethanoid glycosides and one new lignan glycoside. Structures of the compounds were elucidated by spectroscopic methods and chemical evidence.     

Two Decarestrictine Analogs from the Soil-Derived Fungus Penicillium sp. YUD18003 Associated with Gastrodia elata

Two new decarestrictine analogs decarestrictine P and penicitone, together with eight known homologous compounds were isolated from the soil fungus from the rhizosphere of Penicillium sp. YUD18003 related to Gastrodia elata. Their different structures include a decanolides decartestridine P and a long-chain polyhydroxyketone penicitone. The structures of new compounds were determined by nuclear magnetic resonance (NMR) spectroscopic analysis and high resolution electrospray ionization mass spectrometry (HR-ESI-MS), while their absolute configurations were determined by spectroscopic methods, DP4+ probability analysis, modified Snatzke's method and electron circular dichroism (ECD) calculations. All compounds were evaluated for antimicrobial activities.     

Chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus Phomopsis sp. ZSU-H76

Three metabolites named phomopsin A (1), B (2) and C (3), together with two known compounds cytosporone B (4) and C (5), were isolated from the mangrove endophytic fungus, Phomopsis sp. ZSU-H76 obtained from the South China Sea. Their structures were elucidated by spectroscopic methods, mainly by 1D and 2D NMR spectroscopic techniques. The medium-sized cyclic phenol ether based on 1 or 2 is rare in natural products. In bioassays, compounds 1, 2, and 3 had no significant antibiotic activities, but compounds 4 and 5 inhibited two fungi Candida albicans and Fusarium oxysporum with an MIC ranging from 32 to 64 microg/ml.     

Labdane diterpenes from Juniperus communis L. berries

A phytochemical study of the methanol extract of Juniperus communis berries was undertaken. The crude extract was analysed by HPLC-UV and the isolation of the minor compounds was performed by centrifugal partition chromatography. By this means, five diterpenes were isolated, one of which was a new labdane diterpene 15,16-epoxy-12-hydroxy-8(17),13(16),14-labdatrien-19-oic acid. The structures of the isolated compounds were elucidated by spectroscopic methods, including UV, NMR, MS and HR-MS.     

Phytochemical and chemotaxonomic studies on Libanotis laoshanensis W. Zhou et Q. X. Liu

Sixteen compounds, including one polyacetylene (1), twelve coumarins (2–7, 9–14), one sterol (8), as well as two sugar derivatives (15, 16), were obtained from Libanotis laoshanensis by modern phytochemical isolation methods such as column chromatography and preparative TLC. Their structures were identified by spectroscopic methods and in comparison with the published data in the references. All the compounds were reported from L.laoshanensis for the first time. The chemotaxonomic significance of these compounds was also discussed, which demonstrated the relationships between L. laoshanensis and some other species of Apiaceae.     

Antimicrobial constituents from the stem bark of Feronia limonia.

The stem bark of Feronia limonia (Fam. Rutaceae) yielded (-)-(2S)-5,3'-dihydroxy-4'-methoxy-6",6"-dimethylchromeno-(7,8,2",3")-flavanone along with several known compounds including an alkaloid, five coumarins, a flavanone, a lignan, three sterols and a triterpene. The structures of these compounds were determined by spectroscopic methods, mainly 1D and 2D NMR. The antimicrobial screening of compounds by a microdilution technique resulted in MICs in the range 25-100 microg/ml. Other biological activities of the known compounds are also discussed.     

Prenylated p-coumarates from the twigs of Phebalium rude subsp. amblycarpum (Rutaceae)

Phebarudol, a novel prenylated p-coumarate, was isolated from the twigs of Phebalium rude Bartl. subsp. amblycarpum (F. Muell.) P. G. Wilson (Rutaceae) together with the two already known related compounds, werneria chromene and methyl demethoxywutaiensate. The structure of phebarudol was established by spectroscopic methods.     

Neue norkauren- und thymol-derivate aus Athrixia-arten

Investigation of seven Athrixia species affords besides known compounds four new norkaurene derivatives and six thymol derivatives. The structures are elucidated mainly by spectroscopic methods, together with some chemical transformations. Due to nonplanarity of the chromophoric system, one of the compounds shows some surprising spectroscopic data. The chemotaxonomic aspects of these findings are discussed briefly.     

Diterpenoids from Euphorbia paralias.

Two segatane diterpenoids containing a bicyclic [4,3,1] ring system were isolated, together with 11 known diterpenoids, four segetanes, five jatrophanes and two paralianes, from the aerial parts of Euphorbia paralias. The structures of the new compounds were established by spectroscopic means, including by 2D NMR and CD spectroscopic analyses. The antifeedant and antiviral activities of the isolated compounds are also described.     

Lycopodium alkaloids from Lycopodium obscurum L. f. strictum

Two new Lycopodium alkaloids, strictumines A (1) and B (2), along with 18 related known compounds were isolated from the whole plant of Lycopodium obscurum L. f. strictum. The structures of the new compounds were established by spectroscopic methods and chemical transformation. This is the first report on chemical constituents of the title plant. All the isolates were evaluated for their inhibitory activity against acetylcholinesterase (AChE), and only a few were found to show slight bioactivity.     

Two novel cytotoxic and antimicrobial triterpenoids from Pseudolarix kaempferi.

Two novel antimicrobial and cytotoxic triterpenoids, isopseudolarifuroic acids A (1) and B (2), were isolated from the bark of Pseudolarix kaempferi. The structural elucidation of two novel compounds was carried out mainly by spectroscopic methods, and also by computer modeling. Compounds 1 and 2 exhibited significant cytotoxic activities against several tumor cell lines. Compound 1 also showed most potent antimicrobial activities against both Gram-positive and Gram-negative bacteria.     

Three new jatrophane diterpenoids from Euphorbia peplus Linn. with activity towards autophagic flux

Three undescribed jatrophane diterpenoids, named euphpepluones P-R (1–3), were isolated from the whole plant Euphorbia peplus. The structures of these compounds were elucidated by spectroscopic methods. The absolute configuration of 1 was further assigned by X-ray crystallographic analysis. All compounds were evaluated for bioactivity towards autophagic flux by flow cytometry using HM mCherry-GFP-LC3 cells. Compounds 2 and 6 exhibited significant inhibition of autophagic flux.     

苦木化学成分研究

运用各种柱色谱和重结晶等方法对苦木Picrasma quassioides(D.Don)Benn.进行分离纯化,得到五个化合物。通过NMR等现代波谱学方法和技术鉴定化合物的结构分别为:高丽槐素-3-O-β-D-葡萄糖苷(trifolirhizin,1),高丽槐素(maackiain,2),3’,7-二羟基-4’-甲氧基异黄酮(3’,7-dihydroxy-4’-methoxyisoflavone,3),7-羟基香豆素(umbelliferone,4)和大黄素(emodin,5)。五个化合物均为首次从该属植物中分离得到。     

Three clerodane diterpenoids from Croton eluteria Bennett.

Three furanoid clerodanes have been isolated from the stem bark of Croton eluteria Bennett. Their structures have been established by spectroscopic methods. The compounds were named cascarillin B (7alpha-acetoxy-3,4,15,16-diepoxy-12-oxo-cleroda-13(16),14-dien-20-al), cascarillin C (7alpha-acetoxy-15,16,12,20-diepoxy-20-hydroxy-cleroda-3,4,13(16),14-triene) and cascarillin D (7alpha-acetoxy-3,4,15,16-diepoxy-cleroda-13(16),14-dien-20-al).     

Chemical Constituents of Schisandra rubriflora Rehd.et Wils.

Schisandra rubriflora Rehd. et Wils. is a traditional Chinese medicine. To search for new and bioactive components from traditional Chinese medicines and provide scientific evidence for taxonomy, the chemical constituents ofthe plant were investigated by various column chromatography methods (silica gel,Sephadex LH-20, and RP-18). From the aerial parts ofS. rubriflora, three new megastigmane glycosides,namely (3S, 5R, 6S, 9R)-megastigmane-3, 9-diol 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside](1), 7-megastigmene-3-ol-9-one 3-O-[o-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (2), and megastigmane-3α, 4β, 9ξ-triol 3-O-β-D-glucopyranoside (3), along with 14 known compounds, were isolated.The structures of the new compounds were elucidated by a combination of spectroscopic and chemical methods.     

暖地大叶藓化学成分的研究

从真藓科大叶藓属植物暖地大叶藓Rhodobryum giganteum(schwaegr.)Par.的乙醇提取物中分离得到9个化合物,通过波谱分析鉴定其分别为麦角甾-7,22-双烯-3β,5α,6β-三醇(1),乌苏酸(2),琥珀酸(3),尿嘧啶(4),棕榈酸(5),槲皮素(6),碳二十九烷(7),β-谷甾醇(8),胡萝卜苷(9).化合物1～9均为首次从该植物中分离得到.     

Uncarinic acids: phospholipase Cgamma1 inhibitors from hooks of Uncaria rhynchophylla.

Bioactivity-guided fractionation of the CHCl3 extract from hooks of Uncaria rhynchophylla led to the isolation of two triterpene esters, namely uncarinic acids A (1) and B (2). Their structures were established by spectroscopic and chemical methods. These compounds inhibited phospholipase Cgamma1 with IC50 values of 35.66 and 44.55 microM, respectively.     

Macrolactone glycosides of three lichen acids from Acarospora gobiensis, a lichen of Central Asia.

The compounds isolated from the extract of Central Asian lichen (Acarospora gobiensis H. Magn.) comprised three new glycosides having 18-hydroxy-dihydroalloprotolichesterinic, 18-hydroxy-neodihydroprotolichesterinic and 18-hydroxy-dihydroprotolichesterinic acids as aglycones and a di- or trisaccharide moiety linked at C-18 and at the carboxylic group. These compounds, called gobienines A-C, were found to be di- or trisacharides forming a macrolactone with the aglycone. The structures were elucidated by using extensive spectroscopic analysis (1D and 2D NMR, MS, IR and ORD) and chemical and enzymatic methods.