Notes on fungi from north-east India — XXII some species of hypoxylon from Assam

Summary Hypoxylon stygium (Lév)Sacc.,H. archeri Berk.,H. bovei Speg. var.microspora Miller,H. truncatum (Schw.ex Fr.)Miller,H. nummularium Bull. ex Fr. var.merillii Miller (=Hypoxylon asarcodes (Theissen)Miller?) andH. deustum (Hoff. ex Fr.)Grev. (=Ustulina zonata (Lév.)Sacc.), occurring in the tea gardens of Assam are illustrated and described. In addition, the following species ofHypoxylon hitherto reported from this country are enlisted:H. multiforme Fr. (=H. atropurpureum Fr., =H. hookeri Berk.)H. fragiforme (Pers. ex Fr.)Kickx. (=H. coccineum Bull.),H. rubiginosum Pers. ex Fr. (=H. fusco-purpureum (Schw.)Berk. et Curt., =H. nectrioides Speg., =H. perforatum Schw. ex Fr.),H. hypomiltum Mont.,H. indicum (=H. haematostroma Mont.? =H. distillatum Berk. et Fr.),H. investiens (Schw.)Curt.,H. crocopeplum Berk. et Curt. (=H. ochraceo-flavum Berk. et Cooke),H. pistillare Pers. ex Fr.,H. haematostroma Mont. (=H. vividum Berk. et Br.).H. jecorium Berk. et Rav. (=Nummularia cinnabarina P. Henn.),H. suborbiculare (=Nummularia suborbicularis (Welw. et Currey() =H. sclerophaeum Berk. et Curt? Sacc.),H. truncatum (Schw. ex Fr.)Miller (=H. annulatum (Schw.)Mont.) andH. stygium (Lév.)Sacc.     

Deuteromycetes from jabalpur-II

Summary In this paper eighteen fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Of these one, viz.Sarcinella palawanensis (Syd.)V. P. Sahni (=Stigmella palawanensis Syd.) is described as a new combination. Among the new fungus records for India may be citedSeptoria cassiicola Kell. &Swingle onCassia fistula L.,Coniothyrium fuckelii Sacc. onAnogeissus latifolia Wall.,Phomopsis bakeri Syd. onFicus bengalensis L.,Cercospora woodfordiae Petch. onWoodfordia fruticosa (L.)Kurz.,Cercospora guanicencis Young onCaesalpinia sepiaria Roxb., andCercospora chevalieri P. Saccardo onAmorphophallus companulatus Blume.Phyllosticta buteae Syd. onButea monosperma (Lam.)Kuntze,Colletotrichum dracaenae-fragrentis (Mori)Petrak &Sydow onDracaena brachystachys Hook.,Pithomyces chartarum (Berk. &Curt.)M. B. Ellis onCassia fistula L. andCassia tora L.,Cladosporium herbarum (Pers.)Link. onButea monosperma (Lam.)Kuntze andCercospora bougainvilleae P. N. Rao onBougainvillea glabra Choisy are new state records.Discosia artocreas Tode exFr. onHolarrhena antidysentrica Wall.,Botryodiplodia theobromae Pat. onButea monosperma (Lam.)Kuntze,Colletotrichum capsici (Syd.)Butler &Bisby onButea monosperma (Lam.)Kuntze,Alternaria tenuis Nees exFr. onYucca alofolia L.,Alternaria tenuissima (Nees exFr.)Wiltshire onBougainvillea glabra Choisy, andCurvularia lunata (Wakker)Boed. onCassia tora L. are new host records.     

Peptidasen

Summary Using fresh frozen (with and without semipermeable membranes), freeze-dried or sections from aldehyde fixed material and hexazotized p-rosaniline for simultaneous coupling more than 20 different unsubstituted or substituted l-amino acid naphthylamides are split especially in the microvilli and/or stereocilia of the small intestine, kidney and epididymis from rats. Further sites of positive reactions can be revealed by l-alanyl, l-leucyl, l-lysyl, ,l-glutamyl, ,l-glutamyl, l-asparaginyl, N-benzoyl-l-arginyl, N-carbobenzoxy-l-arginyl and N-benzoyl-l-phenylalanyl 2-naphthylamide. Among the substituted and unsubstituted peptide 2-naphthylamides l-prolyl-l-arginyl 2-naphthylamide is not hydrolysed in visible amounts; l-arginyl-l-arginyl, l-alanyl-l-arginyl-l-arginyl, l-alanyl-l-leucyl-l-tyrosyl, l-histidyl-l-seryl, l-seryl-l-tyrosyl and l-glycyl-l-phenylalanyl 2-naphthylamide are metabolized in the renal and intestinal brush border; the reaction pattern obtained with N-carbobenzoxy-l-glycyl-l-glycyl-l-arginyl 2-naphthylamide differs from that of N-carbobenzoxy-l-arginyl 2-naphthylamide. In addition l-glycyl-l-prolyl, l-leucyl-l-alanyl, l-lysyl-l-alanyl and l-alanyl-l-phenylalanyl-l-prolyl 2-naphthylamide are also split in the lysosomes of many organs and the secretion granules of gland cells.

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PeptidasenI. Histochemische Untersuchungen mit 2-Naphthylamiden und Hexazonium-p-rosanilin

Zusammenfassung Mit hexazotiertem p-Rosanilin als Simultankuppler werden in frischen (mit und ohne semipermeable Membranen), gefriergetrockneten und in Schnitten von aldehyd-fixiertem Material mehr als 20 unsubstituierte oder substituierte Aminosäure-2-naphthylamide nahezu ausschließlich in den Mikrovilli und/oder Stereocilien von Dünndarm, Nebenhoden und Niere von Ratten umgesetzt. Weitere positive Reaktionsorte liefern l-Alanyl-, l-Leucyl-, l-Lysyl-, ,l-Glutamyl-, ,l-Glutamyl-, l-Asparaginyl-, N-Benzoyl-l-arginyl-, N-Carbobenzoxy-l-arginyl- und N-Benzoyl-l-phenylalanyl-2-naphthylamid. Unter den Peptid-2-naphthylamiden wird l-Prolyl-l-arginyl-2-naphthylamid nicht in sichtbarer Menge hydrolysiert; l-Alanyl-l-arginyl-l-arginyl-, l-Arginyl-l-arginyl-, l-Asparaginyl-l-arginyl-, l-Alanyl-l-leucyl-l-tyrosyl-, l-Histidyl-l-seryl-, l-Seryl-l-tyrosyl- und l-Glycyl-l-phenylalanyl-2-naphthylamid setzen der renale und intestinale Bürstensaum um. N-Carbobenzoxy-l-glycyl-l-glycyl-l-arginyl-2-naphthylamid liefert verglichen mit Benzoyl- und N-Carbobenzoxy-l-arginyl-2-naphthylamid zusätzliche Resultate. Mit l-Glycyl-l-prolyl-, l-Leucyl-l-alanyl-, l-Lysyl-l-alanyl-und l-Alanyl-l-phenylalanyl-l-prolyl-2-naphthylamid reagieren die Lysosomen zahlreicher Organe und Sekretgranula positiv.

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 105)     

Deuteromycetes from Jabalpur-I

Summary In the present paper twelve fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Among these the new host records areMonochaetia carissae Munjal &Kapoor onCarissa spinarum L.,Pestalotia neglecta Thuem. onAtylosia scarabaeoides,Benth.,Pestalotia japonica Syd. onCelastrus paniculatus Willd.,Pestalotia theae Sawada var.minor Stey. onButea monosperma (Lam.)Kuntze,Pestalotia versicolor Speg. onBuchanania lanzan Spreng.,Colletotrichum dematium (Pers. exFr.)Grove onAmorphophallus sp.,Colletotrichum lindemuthianum (Sacc. &Mag.)Bri. &Cav. onCassia tora L.,Coniella diplodiella (Speg.)Petrak &Syd. on.Anogeissus latifolia Wall.,Hendersonula toruloidea Nattrass onPhilodendron bipinnatifidum Schott. andMyrothecium roridum Tode exFr. onCasearia tomentosa roxb. The above fungi include two species (Pestalotia neglecta Thuem. andPestalotia theae Sawada var.minor Stey.) which have been described for the first time from this country.Sphaeropsis tumefasciens Hedges onCitrus medica L. var.acida L. is a new record from this state; andExcipularia narsapurensis Subramanian, also a new record from the state, is reported for the first time on a named host.     

Some results and prospects of the utilization of beneficial entomophagous insects in the control of insect pests in Georgian SSR (USSR)

Résumé Nous avons brièvement indiqué dans cette note les travaux de lutte biologique entrepris en Géorgie par introduction d'entomophages parasites et prédateurs contre des Insectes nuisibles également introduits auparavant. Nous avons particulièrement souligné l'importance deAphelinus mali Muls. contreEriosoma lanigerum Hausm.,Rodalia cardinalis Muls. contreIcerya purchasi Mask.,Cryptolaemus montrouzieri Muls. contrePseudococcus gahani Green,Lindorus lophanthae Blaisdell contre une série de Diaspidides (Aspidiotus hederae Vall.,A. destructor Sign.,Chrysomphalus dictyospermi (Morg.),Aonidiella citrina Coq.),Prospaltella berlesi How. contrePseudaulacaspis pentagona Targ.,Pseudaphycus malinus contrePseudococcus comstocki (Kuw.),Leptomastix dactylopii How. et Leptomastidea abnormis (Gyr.) contrePseudococcus vitis Niet.,Rhizophagus grandis Gyll. contreDendroctonus micans Kugel.      

Myxomycetes from Orissa (India)-III

Summary Six myxomycetous fungi,Cribraria intricata Schrad.,Elaeomyxa miyazakiensis (Emoto)Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan andPhysarella oblonga (Berk. &Curt.)Morgan have been figured and described form Cuttack, Orissa, of whichElaeomyxa miyazakiensis (Emoto) Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan are new records for India.     

A phylogenetic analysis of the lichen familySphaerophoraceae (Caliciales); a new generic classification and notes on character evolution

A phylogenetic analysis of the familySphaerophoraceae (Caliciales, lichenized ascomycetes) has resulted in a new generic classification. Notes on character evolution are given. The generaSphaerophorus s. str.,Bunodophoron andLeifidium, gen. nov., are accepted.Pleurocybe andPseudosphaerophorus are considered synonyms ofBunodophoron andThysanophoron is considered synonym toSphaerophorus. The following new combinations are proposed:Bunodophoron coomerense (Ohlsson)Wedin,B. diplotypum (Vain.)Wedin,B. dodgei (Ohlsson)Wedin,B. flaccidum (Kantvilas & Wedin)Wedin,B. formosanum (Zahlbr.)Wedin,B. imshaugii (Ohlsson)Wedin,B. insigne (Laurer)Wedin,B. kinabaluense (M. Satô)Wedin,B. macrocarpum (Ohlsson)Wedin,B. madagascareum (Nyl.)Wedin,B. microsporum (Ohlsson)Wedin,B. murrayi (Ohlsson)Wedin,B. notatum (Tibell)Wedin,B. ohlssonii (Wedin)Wedin,B. patagonicum (C. W. Dodge)Wedin,B. ramuliferum (I. M. Lamb)Wedin,B. scrobiculatum (C. Bab.)Wedin,B. tibellii (Wedin)Wedin,B. whakapapaense (Wedin)Wedin, andLeifidium tenerum (Laurer)Wedin.     

Deuteromycetes from Jabalpur. III

Summary In this paper the author has described seventeen new Deuteromycetes from Jabalpur (M.P.) India. These are: Phyllosticta careyae Sahni onCareya arborea Roxb.,Phyllosticta anogeissi Sahni onAnogeissus latifolia Wall.,Phomopsis dalbergiae Sahni onDalbergia sissoo Roxb.,Phomopsis yuccae Sahni onYucca aloifolia L.,Phomopsis dracaenae Sahni onDracaena brachystachys Hook.,Phomopsis buteae Sahni onButea monosperma (Lam.)Kunze,Cytospora grevilleae Sahni onGrevillea robusta A. Cunn.,Coniothyrium sarcinellae Sahni onSarcinella palawanensis (Syd.)Sahni parasitising leaves ofCalastrus paniculatus Willd.,Coniothyrium dioscoreae Sahni on fruits ofDioscorea sp.,Amerodiscosiella indica Sahni onIxora parviflora Vahl.,Ascochyta nyctanthis Sahni onNyctanthes arbor-tristis L.,Pseudodiplodia buteae Sahni onButea monosperma (Lam.)Kunze,Pseudodiplodia oreodoxae Sahni onOreodoxa oleracea Mart.,Phaeoseptoria bougainvilleae Sahni onBougainvillea glabra Choisy,Hainesia jabalpurensis Sahni onWoodfordia fruticosa (L.)Kurz.,Colletotrichum arjunae Sahni onTerminalia arjuna W. & A., andColletotrichum terminaliae Sahni onTerminalia bellirica (Gaertn.)Roxb.     

Some foliicolous ectoparasites and associated fungi from Jabalpur (M.P.) — I

Summary The present paper describes nine ectoparasitic foliicolous fungi from Jabalpur (M.P.) India. These include four new species viz.Asterina woodfordiae Sahni onWoodfordia fruticosa (L.)Kurz.,Schiffnerula fici Sahni, onFicus infectoria Roxb.,Sarcihella fumosus Sahni onAegle marmelos Corr. andSarcinella odinae Sahni onOdina wodier Roxb.,Acremoniella sarcinellae Pat. &Har.,Fumago vagans Pers.,Stigmella palawanensis Syd. andSchiffnerula cassiae are new fungus records for this country.Mitteriella zizyphina Syd. has been recorded onZizyphus xylopyra Willd., for the first time from this state.Z. xylopyra is a new host record forM. zizyphina.     

Some foliicolous ectoparasites and associated fungi from Jabalpur (M.P.) II

Summary In this paper the author has described eight more interesting foliicolous ectoparasites from Jabalpur (M.P.) India. Out of thesePyrenochaeta mitteriellae Sahni an hyperparasite onMitteriella zizyphina Syd. colonising leaves ofZizyphus xylopyra Willd.,Chaetothyrium jasminicola Sahni onJasminum sambac Ait., andMyiocopron parviflorae Sahni onIxora parviflora Vahl are reported as new species.Micropeltis ekmanii Petr. &Cif. on leaves ofCarissa spinarum L.,Asterinella ixorae Ryan on leaves ofIxora parviflora Vahl andPlenotrichum sp. on leaves ofCasearia tomentosa Roxb. are three new fungus records for this country. The remaining two fungi viz.Asterina tonduzi (Speg.)Syd. onFlacourtia ramontchi L'Herr. andBalladynopsis negrii (Cast.) M. B.Ellis onRandia dumetorum Lamk. are new state reocrds.     

On the Lichen generaPlacocarpus andPlacidiopsis (Verrucariaceae)

Verrucaria schaereri (Fr.)Nyl. is accorded generic status asPlacocarpus Trev. emend.O. Breuss. The main differences between the two genera concern the thallus anatomy and the spores which are halonate inPlacocarpus. The following new combinations are proposed:Placocarpus schaereri (Fr.)O. Breuss andPlacidiopsis cinerascens (Nyl.)O. Breuss.     

Substrate specificity of a recombinant ribose-5-phosphate isomerase from <Emphasis Type="Italic">Streptococcus pneumoniae</Emphasis> and its application in the production of <Emphasis Type="SmallCaps">l</Emphasis>-lyxose and <Emphasis Type="SmallCaps">l</Emphasis>-tagatose

A putative ribose-5-phosphate isomerase (RpiB) from Streptococcus pneumoniae was purified with a specific activity of 26.7 U mg⁻¹ by Hi-Trap Q HP anion exchange and Sephacryl S-300 HR 16/60 gel filtration chromatographies. The native enzyme existed as a 96-kDa tetramer with activity maxima at pH 7.5 and 35°C. The RpiB exhibited isomerization activity with l-lyxose, l-talose, d-gulose, d-ribose, l-mannose, d-allose, l-xylulose, l-tagatose, d-sorbose, d-ribulose, l-fructose, and d-psicose and exhibited particularly high activity with l-form monosaccharides such as l-lyxose, l-xylulose, l-talose, and l-tagatose. With l-xylulose (500 g l⁻¹) and l-talose (500 g l⁻¹) substrates, the optimum concentrations of RpiB were 300 and 600 U ml⁻¹, respectively. The enzyme converted 500 g l⁻¹ l-xylulose to 350 g l⁻¹ l-lyxose after 3 h, and yielded 450 g l⁻¹ l-tagatose from 500 g l⁻¹ l-talose after 5 h. These results suggest that RpiB from S. pneumoniae can be employed as a potential producer of l-form monosaccharides.     

Miscellanea hepaticologica 101–110

Summary 101.Blepharidophyllum clandestinum (Mont.)Lacoutore 1910 instead of (Mont.)Grolle 1965. 102.Clasmatocolea rotata (H. f & T. 1844) c. n.=C. alpina (Rodway 1916)Grolle 1966, syn. nov. 103.Isotachis armata (Nees 1844)Gottsche 1864=Lophocolea laxissima Herz. 1932, syn. nov. 104.Leptodon smithii (Hedw.)Web. &Mohr 1803=Jungermannia nervosa Spreng. 1827, syn. nov. 105.Leptoscyphus abditus (Sull.)Dugas 1928 instead of (Sull.)Grolle 1962. 106.L. amphibolius (Nees 1833)Grolle 1962=Chiloscyphus dorsilobus Nees 1845, syn. nov. 107.L. cuneifolius (Hook.)Mitt. new to Venezuela. 108.L. horizontalis (Hook.)Kühnemann 1937 instead of (Hook.)Grolle1962. 109.L. porphyrius (Nees 1845) c. n.=Jungermannia paupercula Tayl. 1846, syn. nov.;=J. liebmaniana L. & G. 1847, syn. nov.=L. liebmanianus (L. & G.)Mitt. 110.Lethocolea amplexifolia (Hampe exLehm.) C. n.=Plagiochila amplexifolia Hampe Ex Lehm. 1838. Notes on marsupia and gemmae ofLethocolea and their taxonomic value.     

Metabolism of amino acid amides in Pseudomonas putida ATCC 12633

The metabolism of the natural amino acid l-valine, the unnatural amino acids d-valine, and d-, l-phenyglycine (d-, l-PG), and the unnatural amino acid amides d-, l-phenylglycine amide (d-, l-PG-NH₂) and l-valine amide (l-Val-NH₂) was studied in Pseudomonas putida ATCC 12633. The organism possessed constitutive l-amidase activities towards l-PG-NH₂ and l-Val-NH₂, both following the same pattern of expression, suggesting the involvement of similarly regulated enzymes, or a common enzyme. Quite surprisingly, growth in mineral media with l-PG-NH₂ resulted in variable, long lag phases of growth and strongly reduced l-amidase activities. Conversion of d-PG-NH₂ into d-PG and l-PG also occurred and could be attributed to the presence of an inducible d-amidase and the racemization of the amino acid amide in combination with l-amidase activity, respectively. The further degradation of l-PG and d-PG involved constitutive l-PG aminotransferase and inducible d-PG dehydrogenase activities, respectively, both with a high degree of enantioselectivity. Amino acid racemase activity for d- and l-PG was not detected. Correspondence to: L. Dijkhuizen     

Bemerkungen zu einigenStipagrostis-Arten (Gramineae) aus Afrika und Arabien

Summary A new species from Egypt (Sinai) and Arabia is described asStipagrostis multinerva H.Scholz. Stipagrostis arabiaefelicis Bor 1967 is a synonym ofSt. drarii (V.Täckholm)de Winter 1963 (Aristida drarii V.Täckholm 1941). The taxonomic status ofStipagrostis foexiana (Maire etWilczek)de Winter (Aristida obtusa subsp.pubescens Andreánszky) in North and South Africa is discussed. Furthermore some new data on taxonomy and distribution of the following species are recorded:Stipagrostis acutiflora subsp.algeriensis (Henrard) comb, nov. et stat. nov.,St. ciliata (Desf.)de Winter,St. lanata (Forsk.)de Winter,St. libyca (H.Scholz) comb, nov.,St. papposa (Trin. etRupr.)de Winter,St. raddiana (Savi)de Winter,St. shawii (H.Soholz) comb, nov., andSt. sokotrana (Vierhapper)de Winter.      

Additions to the fungi of Jabalpur (madhya pradesh) V

Summary The present paper includes ten parasitic fungi occurring at Jabalpur. These include three new species viz.Hendersonia syzygii Hasija onSyzygium cumini, Phoma ixorae Hasija onIxora sp. andPhyllosticta agarwalii Hasija onDalbergia paniculata. Colletotrichum truncatum (Schw.)Andrus &Moore onAlysicarpus bupleurifolius andPiricularia zingiberi onHedychium sp. are new fungus records for the country.Grewia hirsuta forPhyllosticta sedgwickii DaCosta &Mundkur,Lagerstroemia speciosa forPestalotiopsis versicolor (Speg.)Stey.,Zingiber officinale forCurvularia lunata (Wakker)Boedijn,Musa paradisiaca forDeightoniella torulosa (Syd.)Ellis andFlacourtia ramontchi forAlternaria tenuis Nees exPers. are new hosts record from Jabalpur, India.     

Fungi causing plant diseases at Jabalpur (M.P.) XI

Summary In this paper eight foliicolous fungi have been described from Jabalpur (M.P.) India. Out of these, two viz.Phoma jasminicola Agarwal &Sahni onJasminum sambac Ait. andPhyllostictina anthocephali Agarwal &Sahni onAnthocephalus indicus Rich. are new species and three, viz.Pestalotia algeriensis (Sacc. &Berl.)Guba onBorassus flabellifer L.,Pestalotia sorbi Pat. onCarissa spinarum A.DC. andBartalinia robillardioides Tassi onJasminum sambac Ait. andCarissa carandas L. are new fungus records for this country.Colletotrichum dematium (Pers. exFr.)Grove onJasminum sambac Ait.,Glomerella cingulata (Stonem.)Spauld. &Schr. onFicus glomerata Roxb. andIchnocarpus frutescens Br. are new host records.Stigmina phaeocarpa (Mitter)Ellis onBauhinia variegata L. is a new fungus record for the state.     

Zur taxonomischen Stellung und Nomenklatur vonMicropus longifolius (Compositae-Inuleae)

Zusammenfassung Die unter den NamenMicropus longifolius Boiss. etReut. bzw.Cymbolaena longifolia (Boiss. etReut.)Smoljan. gut bekannte Art wurde zuerst alsStylocline griffithii A.Gray beschrieben. Ihre taxonomische Stellung in bezug auf die GattungenMicropus undStylocline wird diskutiert. Es erscheint am richtigsten, die Art in eine eigene monotypische Gattung zu stellen, ihr korrekter Name ist dannCymbolaena griffithii (A.Gray)Wagenitz, comb. nov.

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Summary The species usually known asMicropus longifolius Boiss. etReut. orCymbolaena longifolia (Boiss. etReut.)Smoljan. was described for the first time in 1873 asStylocline griffithii A.Gray. The taxonomic position in relation to the generaMicropus andStylocline is discussed. It seems most appropriate to regard this species as belonging to a monotypic genus. In this case its correct name isCymbolaena griffithii (A.Gray)Wagenitz, comb. nov.

     

Myxomycetes from orissa (india) II

Summary Six myxomycetous fungi,Arcyria denudata (Linn.)Wettstein,Arcyria cinerea (Bull.)Pers.,Hemitrichia stipitata (Massee)Macbr.,Diachea leucopodia (Bull.)Rost.,Stemonitis splendens Rost. andPhysarum nicaraguense Macbr. have been figured and described from Cuttack, Orissa.     

Regulation of reduced nitrogen assimilation in Rhodobacter capsulatus E1F1

The phototrophic bacterium Rhodobacter capsulatus E1F1 assimilates ammonia and other forms of reduced nitrogen either through the GS/GOGAT pathway or by the concerted action of l-alanine dehydrogenase and aminotransferases. These routes are light-independent and very responsive to the carbon and nitrogen sources used for cell growth. GS was most active in cells grown on nitrate or l-glutamate as nitrogen sources, whereas it was heavily adenylylated and siginificantly repressed by ammonium, glycine, l-alanine, l-aspartate, l-asparagine and l-glutamine, under which conditions specific aminotransferases were induced. GOGAT activity was kept at constitutive levels in cells grown on l-amino acids as nitrogen sources except on l-glutamine where it was significantly induced during the early phase of growth. In vitro, GOGAT activity was strongly inhibited by l-tyrosine and NADPH. In cells using l-asparagine or l-aspartate as nitrogen source, a concerted induction of l-aspartate aminotransferase and l-asparaginase was observed. Enzyme level enhancements in response to nitrogen source variation involved de novo protein synthesis and strongly correlated with the cell growth phase.Abbreviations ADH l-alanine dehydrogenase - AOAT l-alanine:2-oxoglutarate aminotransferase - Asnase l-asparaginase - GOAT Glycine: oxaloacetate aminotransferase - GOGAT Glutamate synthase - GOT l-aspartate: 2-oxoglutarate aminotransferase - GS Glutamine synthetase - HPLC High-Pressure Liquid Chromatography - MOPS 2-(N-morpholino)propanesulfonic acid - MSX l-methionine-d,l-sulfoximine