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1.
Detached leaves of pea (Pisum sativum) were submitted to water stress at different relative air humidities. The photosynthetic
activity of photosystem 2 (PS2) was monitored by time-resolved picosecond chlorophyll (Chl) fluorescence spectroscopy. In
the first days the well-known fast Chl fluorescence decay was observed which indicated high PS2 activity. After a few days
the average fluorescence decay time τm reached a maximum, depending on the wilting conditions, but always at a relative loss
of leaf mass of 80%. After this maximum, τm decreased within a few hours, the fluorescence decay became similar to that one
of an intact leaf, but an additional fluorescence decay component with a lifetime of 3.6 ns appeared. At first the primary
quinone QA was reduced due to inhibition of the electron transfer to the secondary quinone QB. Simultaneously, water deficiency
caused an electron lack at the oxidizing site of PS2. This disabled the primary electron donor of PS2, tyrosine Z, from reducing
the oxidized reaction centre of PS2 (P680+). Thus a recombination of P680+-pheophytin-QA- took place, and the energy was lost
as heat. With further water stress, QA was decoupled from PS2. The new fluorescence decay component could therefore be assigned
to energetically decoupled antenna complexes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
The effects of several respiration inhibitors on photosystem II (PS II) were investigated. Among the agents tested, piericidin A and thenoyltrifluoroacetone (TTFA) inhibited the photosynthetic electron transport of spinach as measured from chlorophyll (Chl) fluorescence parameters (Fm'-F)/Fm' and Fv/Fm. Using specific donors and acceptors of electrons, we identified the sites of inhibition in and around the PS II complex; the site of inhibition by TTFA was between QA, primary quinone acceptor in PS II, and QB, secondary quinone acceptor, in the acceptor side of P680, the reaction center Chl of PS II, while inhibition by piericidin A of the acceptor side was downstream of Q(B), out of the PS II complex. Both agents also inhibited the donor side of P680, probably between tyrosine-161 of the reaction center protein of PS II and P680. 相似文献
3.
Electron transfer processes in leaves were investigated by chlorophyll fluorescence decay measurements. A fast chlorophyll fluorescence decay was observed in the intact state, reflecting normal electron transfer in Photosystem II. After treatment with DCMU a slow chlorophyll fluorescence decay was measured due to blocked electron transfer after the primary quinone QA. Additional saturating light pulses, one between each two measuring pulses, were used to completely reduce QA of the intact leaf: the chlorophyll fluorescence decay became similar to that of a DCMU treated leaf. A decreased electron donation rate to the reaction centre P680 was obtained after treatment with hydroxylamine. The intensity of the additional saturating light pulses was not sufficient to reduce all QA under this condition and only a small increase of the average chlorophyll fluorescence decay time occurred. Following our previous paper [Berg et al. (1997) Photosynthetica 34, in press], we investigated the effects of water stress with the additional saturating light pulses. An almost complete reduction of QA was possible after water stress started. A small, but systematic shortening of the slow chlorophyll fluorescence decay followed, up to a relative loss of leaf mass of 80%. At this time a rapid shortening of the chlorophyll fluorescence decay occurred, caused by an electron deficiency at the donor site of PS II. Additional saturating light pulses had no effects on the chlorophyll fluorescence decay any more, revealing a radiationless recombination between the reduced primary quinone Q
and the oxidized reaction centre P680+. 相似文献
4.
Chlorophyll a fluorescence transient as an indicator of active and inactive Photosystem II in thylakoid membranes 总被引:10,自引:0,他引:10
J Cao 《Biochimica et biophysica acta》1990,1015(2):180-188
Upon illumination, a dark-adapted photosynthetic sample shows time-dependent changes in chlorophyll (Chl) a fluorescence yield, known as the Kautsky phenomenon or the OIDPS transient. Based on the differential effects of electron acceptors such as 2,5-dimethyl-p-benzoquinone (DMQ) and 2,6-dichloro-p-benzoquinone (DCBQ) on Chl a fluorescence transients of spinach thylakoids, we suggest that the OID phase reflects the reduction of the electron acceptor QA to QA- in the inactive PS II (see Graan, T. and Ort, D. (1986) Diochim. Biophys. Acta 852, 320-330). In spinach thylakoids, heat-induced increase of the Chl a fluorescence yield is also differentially sensitive to the addition of DMQ and DCBQ suggesting that this increase is mainly on the 'I' level, and thus heating is suggested to convert active PS II to inactive PS II centers. The kinetics of decay of QA-, calculated from variable Chl a fluorescence, was analyzed into three exponential components (365-395 microseconds; 6-7 ms; and 1.4-1.7 s). In heated samples, the decay rate of variable Chl a fluorescence is slower than the normal back-reaction rate; there is a preponderance of the slow component that may be due, partly, to the active centers undergoing slow back reaction between QA- and the S2 state of the oxygen-evolving complex. 相似文献
5.
Photon absorption by one of the roughly 200 chlorophylls of the plant Photosystem II (PSII) results in formation of an equilibrated excited state (Chl200*) and is followed by chlorophyll oxidation (formation of P680+) coupled to reduction of a specific pheophytin (Phe), then electron transfer from Phe- to a firmly bound quinone (QA), and subsequently reduction of P680+ by a redox-active tyrosine residue denoted as Z. The involved free-energy differences (DeltaG) and redox potentials are of prime interest. Oxygen-evolving PSII membrane particles of spinach were studied at 5 degrees C. By analyzing the delayed and prompt Chl fluorescence, we determined the equilibrium constant and thus free-energy difference between Chl200* and the [Z+,QA-] radical pair to be -0.43+/-0.025 eV, at 10 mus after the photon absorption event for PSII in its S(3)-state. On basis of this value and previously published results, the free-energy difference between P680* and [P680+,QA-] is calculated to be -0.50+/-0.04 eV; the free-energy loss associated with electron transfer from Phe to QA is found to be 0.34+/-0.04 eV. The given uncertainty ranges do not represent a standard deviation or likely error, but an estimate of the maximal error. Assuming a QA-/QA redox potential of -0.08 V, the following redox-potential estimates are obtained: +1.25 V for P680/P680+; +1.21 V for Z/Z+ (at 10 mus); -0.42 V for Phe-/Phe; -0.58 V for P680*/P680+. 相似文献
6.
Photosystem II (PS II) is unique among photosynthetic reaction centers in having secondary electron donors that compete with the primary electron donors for reduction of P680(+). We have characterized the photooxidation and dark decay of the redox-active accessory chlorophylls (Chl) and beta-carotenes (Car) in oxygen-evolving PS II core complexes by near-IR absorbance and EPR spectroscopies at cryogenic temperatures. In contrast to previous results for Mn-depleted PS II, multiple near-IR absorption bands are resolved in the light-minus-dark difference spectra of oxygen-evolving PS II core complexes including two fast-decaying bands at 793 and 814 nm and three slow-decaying bands at 810, 825, and 840 nm. We assign these bands to chlorophyll cation radicals (Chl(+)). The fast-decaying bands observed after illumination at 20 K could be generated again by reilluminating the sample. Quantization by EPR gives a yield of 0.85 radicals per PS II, and the yield of oxidized cytochrome b 559 by optical difference spectroscopy is 0.15 per PS II. Potential locations of Chl(+) and Car(+) species, and the pathways of secondary electron transfer based on the rates of their formation and decay, are discussed. This is the first evidence that Chls in the light-harvesting proteins CP43 and CP47 are oxidized by P680(+) and may have a role in Chl fluorescence quenching. We also suggest that a possible role for negatively charged lipids (phosphatidyldiacylglycerol and sulfoquinovosyldiacylglycerol identified in the PS II structure) could be to decrease the redox potential of specific Chl and Car cofactors. These results provide new insight into the alternate electron-donation pathways to P680(+). 相似文献
7.
Diurnal patterns of gas exchange and chlorophyll (Chl) fluorescence parameters of photosystem 2 (PS2) as well as Chl content
were analyzed in Reaumuria soongorica (Pall.) Maxim., a perennial semi-shrub during dehydration and rehydration. The net photosynthetic rate (P
N), maximum photochemical efficiency of PS2 (variable to maximum fluorescence ratio, Fv/Fm), quantum efficiency of non-cyclic electron transport of PS2, and Chl content decreased, but non-photochemical quenching
of fluorescence and carotenoid content increased in stems with the increasing of drought stress. 6 d after re-hydration, new
leaves budded from stems. In the re-watered plants, the chloroplast function was restored and Chl a fluorescence returned to a similar level as in the control plants. This improved hydraulic adjustment in plant triggered
a positive effect on ion flow in the tissues and increased shoot electrical admittance. Thus R. soongorica plants are able to sustain drought stress through leaf abscission and keep part of Chl content in stems. 相似文献
8.
采用相同的分离技术,从水葫芦(Eichhornia crassipes(Mart)Solms.)和菠菜(Spinacia oleracea L.)叶片中提取叶绿体.利用吸收光谱和低温荧光光谱及皮秒荧光单光子计数技术对它们的光谱性质和光系统Ⅱ荧光寿命进行了研究.这两种叶绿体吸收光谱相似,暗示着它们都能高效吸收不同波长的光子.低温荧光光谱显示,水葫芦叶绿体两个光系统之间激发能分配平衡状态差,表明不利于该植物叶绿体高效利用吸收的光子能.采用三指数动力学模型对测定的光系统Ⅱ荧光衰减曲线拟合,水葫芦叶绿体光系统Ⅱ荧光衰减寿命分别是:138,521和1 494 ps;菠菜叶绿体荧光寿命分别是:197,465和1 459ps.并且归属了荧光组分,慢速度荧光衰减是由叶绿素堆积造成的,中等速度荧光衰减源于PSⅡ反应中心重新结合电荷组分,快速度荧光衰减归属于PSⅡ反应中心组分.基于20ps模型计算的水葫芦和菠菜叶绿体PSⅡ反应中心激发能转能效率分别是87%和91%.该结果与转能效率为100%的观点不一致.实验结果支持PSⅡ反应中心电荷分裂20 ps时间常数模型.根据转能效率,水葫芦生长速度不大于菠菜生长速度,但是,水葫芦叶绿体中含有丰富的胡萝卜素成分,其单位质量叶绿体吸收光能大于单位质量菠菜叶绿体吸收的量.实验结果还暗示植物叶绿体体系传能高效,接近于100%. 相似文献
9.
Msilini N Zaghdoudi M Govindachary S Lachaâl M Ouerghi Z Carpentier R 《Photosynthesis research》2011,107(3):247-256
The effect of iron deficiency on photosynthetic electron transport in Photosystem II (PS II) was studied in leaves and thylakoid
membranes of lettuce (Lactuca sativa, Romaine variety) plants. PS II electron transport was characterized by oxygen evolution and chlorophyll fluorescence parameters.
Iron deficiency in the culture medium was shown to affect water oxidation and the advancement of the S-states. A decrease
of maximal quantum yield of PS II and an increase of fluorescence intensity at step J and I of OJIP kinetics were also observed.
Thermoluminescence measurements revealed that charge recombination between the quinone acceptor of PS II, QB, and the S2 state of the Mn-cluster was strongly perturbed. Also the dark decay of Chl fluorescence after a single turnover white flash
was greatly retarded indicating a slower rate of QA
− reoxidation. 相似文献
10.
To understand the origins of the different lifetime components of photosystem 2 (PS2) chlorophyll (Chl) fluorescence we have studied their susceptibility to potassium iridic chloride (K2IrCl6) which has been shown to bleach antenna pigments of photosynthetic bacteria (Loach et al. 1963). The addition of K2IrCl6 to PS2 particles gives rise to a preferential quenching of the variable Chl fluorescence (Fv). At concentrations lower than 20 M, this is brought about mainly by a decrease in the yield, but not in the lifetime, of the slowest component when all the PS2 reaction centres are closed (FM). The yield of the middle and fast decays are not significantly altered. This type of quenching is not seen with DNB. The iridate-induced quenching of the initial fluorescence level (F0) is due to a proportional decrease in the yield and lifetime of the three components and correlates with the observed modification in the relative quantum yield of oxygen evolution. In this concentration range a bleaching of Chl a is seen. At higher iridate levels, greater than 20 M, a proportional decrease in the lifetimes and yields of the three kinetic components is seen at FM. These changes are associated with a carotenoid bleaching. In isolated light harvesting Chl a/b complexes of PS2 (LHC2), iridate addition converts a 4 ns decay into a 200 ps emission and both types of bleaching are observed. By also measuring the rate of PS2 trap closure versus iridate concentration, we have discussed the results in terms of excitation energy transfer.Abbreviations DNB
m-dinitrobenzene
- FM
maximum Chl fluorescence
- F0
initial fluorescence
- Fv
variable fluorescence
- I
pheophytin a primary electron acceptor of PS2
- P680
chlorophyll a of photochemical centre
- PS2
photosystem 2
- QA
primary stable electron acceptor of PS2
- Chl
chlorophyll
- LHC2
light harvesting Chl a/b complex of PS2
- MES
2(N-morpholino) ethanesulfonic acid
- DCMU
3-(3-4-dichlorophenyl) 1-1 dimethylurea
- PPBQ
phenyl-p-benzo-quinone
- BBY
PS2-enriched membranes prepared as in Berthold et al. (1981)
- Q400
PS2 electron acceptor with a midpoint potential of 400 mV 相似文献
11.
Inhibition of photosystem 2 by the peptide-modification reagent, tetranitromethane, has been investigated with spinach digitonin particles. In the presence of tetranitromethane, (1) the initial fluoresence yield is suppressed with a concomitant elimination of the variable component of fluorescence; (2) the optical absorption transient at 820 nm, attributed to P680+, is greatly attenuated; (3) diphenylcarbazide-supported photoreduction of dichlorophenol indophenol is abolished; and (4) electron spin resonance Signal 2f and Signal 2s are eliminated. These results are consistent with multiple sites of modification in photosystem 2 by tetranitromethane, and suggest further that this reagent can inhibit charge stabilization in the reaction center.Abbreviations D1
electron donor to P680+ in oxygen-inhibited photosystem 2 preparations
- DPIP
2,6-dichlorophenol indophenol
- esr
electron spin resonance
- Fi
initial chlorophyll a fluorescence yield
- Fmax
maximum chlorophyll a fluorescence yield
- Fv
variable chlorophyll a fluorescence yield
- FWHM
full width at half maximum
- Mes
2-(N-morpholino)ethanesulfonic acid
- P680
primary electron donor chlorophyll of photosystem 2
- Ph
pheophytin
- PS
2-photosystem 2
- Qa
primary quinone electron acceptor
- Qb
secondary quinone acceptor
- Tricine
N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine
- TNM
tetranitromethane 相似文献
12.
The polyphasic patterns of fluorescence induction rise in pea leaves in vivo and after the treatment with ionophores have been studied using a plant efficiency analyzer. To analyze in detail photosystem II (PS II) electron transfer processes, an extended PS II model was applied, which included the sums of exponential functions to specify explicitly the light-driven formation of the transmembrane electric potential (delta psi(t)) as well as pH in the lumen (pHL(t)) and stroma (pHs(t)). PS II model parameters and numerical coefficients in delta psi(t), and pHs(t) were evaluated to fit fluorescence induction data for different experimental conditions: leaf in vivo or after ionophore treatment at low or high light intensity. The model imitated changes in the pattern of fluorescence induction rise due to the elimination of transmembrane potential in the presence of ionophores, when delta psi = 0 and pHL(t), pHS(t) altered to small extent relative to control values in vivo, with maximum delta psi(t) approximately 90 MB and delta psi(t) approximately 40 MB, for the stationary state at deltapH aproximately equal to 1.8. As the light intensity was increased from 300 to 1200 micromol x m(-2) x s(-1), the heat dissipation rate constants increased threefold for nonradiative recombination of P680+Phe- and by approximately 30% for P680+Q(A)-. The parameters delta psi, pH(S) and pH(L) were analyzed as factors of PS II redox state populations and fluorescence yield. The kinetic mechanism of qE quenching is discussed, which is related with light induced pH(L) lumen acidification, when Q(A)- and P680+ recombination probability increases to regulate the QA reduction. 相似文献
13.
Enhanced photosystem 2 thermostability during leaf growth of Elm (Ulmus pumila) seedlings 总被引:1,自引:0,他引:1
C. -D. Jiang G. -M. Jiang X. Wang L. -H. Li D. K. Biswas Y. -G. Li 《Photosynthetica》2006,44(3):411-418
We examined photosynthetic activities and thermostability of photosystem 2 (PS2) in leaves of elm (Ulmus pumila) seedlings from initiation to full expansion. During leaf development, net photosynthetic rate (P
N) increased gradually and reached the maximum when leaves were fully developed. In parallel with the increase of P
N, chlorophyll (Chl) content was significantly elevated. Chl a fluorescence measurements showed that the maximum quantum yield of PS2 (ϕPS2), the efficiency a trapped exciton, moved an electron into the electron transport chain further than QA
− (Ψo), and the quantum yield of electron transport beyond QA (ϕEo) increased gradually. These results were independently confirmed by our low irradiance experiments. When subjected to progressive
heat stress, the young leaves exhibited considerably lower ϕPS2 and higher minimal fluorescence (F0) than the mature leaves, revealing the highly sensitive nature of PS2 under heat in the newly initiating leaves. Further
analysis showed that PS2 structure in the newly initiating leaves was strongly altered under heat, as evidenced by the increased
fluorescence signals at the position of the K step. We therefore demonstrated an inhibition in the oxygen-evolving complex
(OEC) in the young leaves. This resulted in decrease in amount of the functional PS2 reaction centres and relative increase
in the PS2 reaction centres with inhibited electron transport at the acceptor side under heat. We suggest that the enhanced
thermostability of PS2 during leaf development is associated with improved OEC stability. 相似文献
14.
Markus Grabolle 《BBA》2005,1708(2):209-218
Photon absorption by one of the roughly 200 chlorophylls of the plant Photosystem II (PSII) results in formation of an equilibrated excited state (Chl200*) and is followed by chlorophyll oxidation (formation of P680+) coupled to reduction of a specific pheophytin (Phe), then electron transfer from Phe− to a firmly bound quinone (QA), and subsequently reduction of P680+ by a redox-active tyrosine residue denoted as Z. The involved free-energy differences (ΔG) and redox potentials are of prime interest. Oxygen-evolving PSII membrane particles of spinach were studied at 5 °C. By analyzing the delayed and prompt Chl fluorescence, we determined the equilibrium constant and thus free-energy difference between Chl200* and the [Z+,QA−] radical pair to be −0.43 ± 0.025 eV, at 10 μs after the photon absorption event for PSII in its S3-state. On basis of this value and previously published results, the free-energy difference between P680* and [P680+,QA−] is calculated to be −0.50 ± 0.04 eV; the free-energy loss associated with electron transfer from Phe to QA is found to be 0.34 ± 0.04 eV. The given uncertainty ranges do not represent a standard deviation or likely error, but an estimate of the maximal error. Assuming a QA−/QA redox potential of −0.08 V [Krieger et al., 1995, Biochim. Biophys. Acta 1229, 193], the following redox-potential estimates are obtained: +1.25 V for P680/P680+; +1.21 V for Z/Z+ (at 10 μs); −0.42 V for Phe−/Phe; −0.58 V for P680*/P680+. 相似文献
15.
短期高温胁迫对高产小麦品系灌浆后期旗叶光系统Ⅱ功能的影响 总被引:15,自引:3,他引:12
以叶绿素快相荧光动力学曲线(OJIP)为探针,探讨了高温胁迫对高产小麦品系01-35灌浆后期光系统Ⅱ(PSⅡ)功能的影响.结果表明,在37 ℃~43 ℃范围内,随温度升高QA还原程度和还原速率增大,至43 ℃时分别比室温下增加了23.89%和24.09%,表明QA→QB的电子传递受到抑制;43 ℃时PSⅡ的电子受体库降至室温下的47.4%,表明高温胁迫伤害了PSⅡ受体库;而PSⅡ供体侧未受到影响.当温度达到46 ℃时,QA还原程度和还原速率分别比室温下增加了13.95%和20.48%,但比43 ℃时显著下降,而PSⅡ电子受体库与43 ℃时相比无显著变化,表明46 ℃时PSⅡ供体侧受到伤害.与对照品种鲁麦14相比,高温胁迫下高产小麦的捕光色素复合体仍能捕获较多的光能,而且将捕获的光能更多的用于电子传递,表明高产小麦的捕光色素复合体及电子传递体耐受高温的能力较强,能够维持较高的电子传递能力. 相似文献
16.
Photosynthetic performance and acclimation of <Emphasis Type="Italic">Incarvillea delavayi</Emphasis> to water stress 总被引:1,自引:0,他引:1
The photosynthetic performance and related leaf traits of Incarvillea delavayi Bur. et Franch were studied at different water regimes to assess its capacity for photosynthetic acclimation to water stress. The
initial response of I. delavayi to water stress was the closure of stomata, which resulted in down-regulation of photosynthesis. The stomatal limitation
(SL) represented the main component to photosynthetic limitations but non-stomatal limitation (NSL) increased quickly with the increasing water stress, and had similar magnitude to SL under severe water stress (soil moisture
25–30 % of field capacity). Chlorophyll (Chl) a fluorescence parameters characterizing photosystem (PS) 2 photochemical efficiency (ΦPS2), electron transport rate (J) and photochemical quenching (qP) decreased with the increasing water stress, indicating impaired
photosynthetic apparatus. However, the water-stressed plants had a increased mesophyll CO2 diffusional conductance, Chl a/b ratio, leaf nitrogen partitioning in RuBPCO and bioenergetics in later grown parts, indicating that I. delavay had a substantial physiological plasticity and showed a good tolerance to water stress. 相似文献
17.
Electron paramagnetic resonance (EPR) analyses (g = 2 region) and optical spectrophotometric analyses of P680+ were made of NH2OH-extracted photosystem II (PSII) membranes after various durations of weak-light photoinhibition, in order to identify the sites of damage responsible for the observed kinetic components of the loss of electron transport [Blubaugh, D.J., & Cheniae, G.M. (1990) Biochemistry 29, 5109-5118]. The EPR spectra, recorded in the presence of K3Fe(CN)6, gave evidence for rapid (t1/2 = 2-3 min) and slow (t1/2 = 3-4) losses of formation of the tyrosyl radicals YZ+ and YD+, respectively, and the rapid appearance (t1/2 = 0.8 min) of a 12-G-wide signal, centered at g = 2.004, which persisted at 4 degrees C in subsequent darkness in rather constant abundance (approximately 1/2 spin per PSII). This latter EPR signal is correlated with quenching of the variable chlorophyll a fluorescence yield and is tentatively attributed to a carotenoid (Car) cation. Exogenous reductants (NH2OH greater than or equal to NH2NH2 greater than DPC much greater than Mn2+) were observed to reduce the quencher, but did not reverse other photoinhibition effects. An additional 10-G-wide signal, tentatively attributed to a chlorophyll (Chl) cation, is observed during illumination of photoinhibited membranes and rapidly decays following illumination. The amplitude of formation of the oxidized primary electron donor, P680+, was unaffected throughout 120 min of photoinhibition, indicating no impairment of charge separation from P680, via pheophytin (Pheo), to the first stable electron acceptor, QA. However, a 4-microsecond decay of P680+, reflecting YZ----P680+, was rapidly (t1/2 = 0.8 min) replaced by an 80-140 microsecond decay, presumably reflecting QA-/P680+ back-reaction. Photoinhibition caused no discernible decoupling of the antenna chlorophyll from the reaction center complex. We conclude that the order of susceptibility of PSII components to photodamage when O2 evolution is impaired is Chl/Car greater than YZ greater than YD much greater than P680, Pheo, QA. 相似文献
18.
Fluorescence and electron paramagnetic resonance (EPR) measurements have been applied to characterize chlorophyll triplet formation in the reaction center of photosystem II (PSII). A highly triplet forming state was generated in PSII membranes by chemical double reduction of the primary electron acceptor QA. In triplet forming PSII centers, the steady-state yield of chlorophyll fluorescence decreased to about 70% of the maximal fluorescence yield observed in closed PSII centers in which QA is singly reduced. The results are well interpreted in the framework of a model where the charge state of QA electrostatically controls the yield of primary charge separation [Schatz, G. H., Brock, H., & Holzwarth, A. R. (1988) Biophys. J. 54, 397-405]. Thus, high triplet yield and decreased, although still quite high, fluorescence indicate a charge-neutralized state of PSII in which QA is singly or doubly reduced and protonated or absent. The EPR signal of the triplet primary chlorophyll donor, 3P680, is suppressed by illumination at 77 K concomitant with the formation of a cationic radical (g = 2.0025-2.0027, and 0.92 mT wide) that is stable in the dark. This is attributed to the oxidation of an accessory chlorophyll (Chl) in the vicinity of P680. Electrostatic repulsion between Chl+ and P680+ is likely to prevent primary charge separation, and in turn triplet formation, providing a further example of electrostatic control of primary charge separation. The triplet P680 EPR signal is also suppressed in the presence of oxygen. This effect, which is almost completely reversible by removing the oxygen, is attributed to the interaction of triplet P680 with triplet O2. 相似文献
19.
Interaction of linolenic acid with bound quinone molecules in Photosystem II. Time-resolved optical and electron spin resonance studies 总被引:1,自引:0,他引:1
Time-resolved spectroscopic techniques, including optical flash photolysis and electron spin resonance spectroscopy, have been utilized to monitor electron-transport activity in Photosystem II subchloroplast particles. These studies have indicated that in the presence of 100 microM linolenic acid (1) a high initial fluorescence yield (Fi) is observed upon steady-state illumination of the dark-adapted sample; (2) flash-induced absorption transients (t greater than 10 mus) in the region of 820 nm, attributed to P-680+, are first slowed, then abolished; and (3) electron spin resonance Signal IIs and Signal IIf (Z+) are not detectable. Upon reversal of linolenic acid inhibition by washing with bovine serum albumin, optical and electron spin resonance transients originating from the photooxidation of P-680 are restored. Similarly, the variable component of fluorescence is recovered with an accompanying restoration of Signal IIs and Signal IIf. The data indicate that linolenic acid affects two inhibition sites in Photosystem II: one located between pheophytin and QA on the reducing side, and the other between electron donor Z and P-680 on the oxidizing side. Since both sites are associated with bound quinone molecules, we suggest that linolenic acid interacts at the level of quinone binding proteins in Photosystem II. 相似文献
20.
Energy transfer and distribution in the red alga Porphyra perforata studied using picosecond fluorescence spectroscopy 总被引:1,自引:0,他引:1
The detailed process of excitation transfer among the antenna pigments of the red alga Porphyra perforata was investigated by measuring time-resolved fluorescence emission spectra using a single-photon timing system with picosecond resolution. The fluorescence decay kinetics of intact thalli at room temperature revealed wavelength-dependent multi-component chlorophyll a fluorescence emission. Our analysis attributes the majority of chlorophyll a fluorescence to excitation originating in the antennae of PS II reaction centers and emitted with maximum intensities at 680 and 740 nm. Each of these fluorescence bands was characterized by two kinetic decay components, with lifetimes of 340-380 and 1700-2000 ps and amplitudes varying with wavelength and the photochemical state of the PS II reaction centers. In addition, a small contribution to the long-wavelength fluorescence band is proposed to arise from chlorophyll a antennae coupled to PS I. This component displays fast decay kinetics with a lifetime of approx. 150 ps. Desiccation of the thalli dramatically increases the contribution of this fast decay component. 相似文献