首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
We tested the involvement of ethylene in maize (Zea mays L.) root gravitropism by measuring the kinetics of curvature and lateral auxin movement in roots treated with ethylene, inhibitors of ethylene synthesis, or inhibitors of ethylene action. In the presence of ethylene the latent period of gravitropic curvature appeared to be increased somewhat. However, ethylene-treated roots continued to curve after control roots had reached their final angle of curvature. Consequently, maximum curvature in the presence of ethylene was much greater in ethylene-treated roots than in controls. Inhibitors of ethylene biosynthesis or action had effects on the kinetics of curvature opposite to that of ethylene, i.e. the latent period appeared to be shortened somewhat while total curvature was reduced relative to that of controls. Label from applied 3H-indole-3-acetic acid was preferentially transported toward the lower side of stimulated roots. In parallel with effects on curvature, ethylene treatment delayed the development of gravity-induced asymmetric auxin movement across the root but extended its duration once initiated. The auxin transport inhibitor, 1-N-naphthylphthalamic acid reduced both gravitropic curvature and the effect of ethylene on curvature. Since neither ethylene nor inhibitors of ethylene biosynthesis or action prevented curvature, we conclude that ethylene does not mediate the primary differential growth response causing curvature. Because ethylene affects curvature and auxin transport in parallel, we suggest that ethylene modifies curvature by affecting gravity-induced lateral transport of auxin, perhaps by interfering with adaptation of the auxin transport system to the gravistimulus.  相似文献   

2.
Parker KE  Briggs WR 《Plant physiology》1990,94(4):1763-1769
We have investigated the transport of tritiated indole-3-acetic acid (IAA) in intact, red light-grown maize (Zea mays) coleoptiles during gravitropic induction and the subsequent development of curvature. This auxin is transported down the length of gravistimulated coleoptiles at a rate comparable to that in normal, upright plants. Transport is initially symmetrical across the coleoptile, but between 30 and 40 minutes after plants are turned horizontal a lateral redistribution of the IAA already present in the transport stream occurs. By 60 minutes after the beginning of the gravitropic stimulus, the ratio of tritiated tracer auxin in the lower half with respect to the upper half is approximately 2:1. The redistribution of growth that causes gravitropic curvature follows the IAA redistribution by 5 or 10 minutes at the minimum in most regions of the coleoptile. Immobilization of tracer auxin from the transport stream during gravitropism was not detectable in the most apical 10 millimeters. Previous reports have shown that in intact, red light-grown maize coleoptiles, endogenous auxin is limiting for growth, the tissue is linearly responsive to linearly increasing concentrations of small amounts of added auxin, and the lag time for the stimulation of straight growth by added IAA is approximately 8 or 9 minutes (TI Baskin, M Iino, PB Green, WR Briggs [1985] Plant Cell Environ 8: 595-603; TI Baskin, WR Briggs, M Iino [1986] Plant Physiol 81: 306-309). We conclude that redistribution of IAA in the transport stream occurs in maize coleoptiles during gravitropism, and is sufficient in degree and timing to be the immediate cause of gravitropic curvature.  相似文献   

3.
Re-orientation of Arabidopsis seedlings induces a rapid, asymmetric release of the growth regulator auxin from gravity-sensing columella cells at the root apex. The resulting lateral auxin gradient is hypothesized to drive differential cell expansion in elongation-zone tissues. We mapped those root tissues that function to transport or respond to auxin during a gravitropic response. Targeted expression of the auxin influx facilitator AUX1 demonstrated that root gravitropism requires auxin to be transported via the lateral root cap to all elongating epidermal cells. A three-dimensional model of the root elongation zone predicted that AUX1 causes the majority of auxin to accumulate in the epidermis. Selectively disrupting the auxin responsiveness of expanding epidermal cells by expressing a mutant form of the AUX/IAA17 protein, axr3-1, abolished root gravitropism. We conclude that gravitropic curvature in Arabidopsis roots is primarily driven by the differential expansion of epidermal cells in response to an influx-carrier-dependent auxin gradient.  相似文献   

4.
To investigate the spatial and temporal dependence of hormonal regulation during gravitropism, we compared the effects of root cap application of indole-3-acetic acid (IAA) and abscisic acid (ABA) with gene expression changes occurring naturally during gravitropic reaction of Brassica rapa roots. The expression of auxin, ABA, and metabolism-related genes in the tip, elongation zone, and maturation zone varied with time, location, and hormone concentration and characterized polar auxin transport. IAA was transported readily shootward and inhibited growth more than ABA. Expression of PIN3 and IAA5 in the elongation zone showed downregulation on the convex but upregulation on the concave side. Both PIN7 and IAA5 responded near maximally to 10?8 M IAA within 30 min, suggesting that auxin activates its own transport system. Ubiquitin 1 (UBQ1) responded after a lag time of more than 1 h to IAA. The metabolic control gene Phosphoenolpyruvate carboxylase 1 (PEPC1) was more sensitive to ABA but upregulated by high concentrations of either hormone. The time course and duration of gene activation suggests that ABA is not involved in gravitropic curvature, differential elongation is not simply explained by IAA-induced upregulation, and that reference genes are sensitive to auxin.  相似文献   

5.
Kim SK  Chang SC  Lee EJ  Chung WS  Kim YS  Hwang S  Lee JS 《Plant physiology》2000,123(3):997-1004
Exogenously applied brassinolide (BL, 10(-9)-10(-5) M) increased gravitropic curvature in maize (Zea mays) primary roots. The BL-enhanced gravitropic curvature was clearly promoted in the presence of indole-3-acetic acid (IAA, 10(-10)-10(-8) M), indicating that BL is interactive with IAA during the gravitropic response. The interactive effect between BL and IAA was completely diminished by treatment of p-chlorophenoxy isobutric acid, an auxin action antagonist. The activation of the gravitropic response by BL in the absence and in the presence of IAA was nullified by application of 2, 3,5-triiodobenzoic acid, a polar auxin transport inhibitor. The data indicate that brassinosteroids (BRs) might be involved in auxin-mediated processes for the gravitropic response. Gas chromotography-selected ion-monitoring analysis revealed that maize primary roots contained approximately 0.3 ng g(-1) fresh weight castasterone as an endogenous BR. Exogenously applied castasterone also increased the gravitropic response of maize roots in an IAA-dependent manner. This study provides the first evidence, to our knowledge, for occurrence and gravitropic activity of BRs in plant roots.  相似文献   

6.
Primary roots of maize (Zea mays L.) and pea (Pisum sativum L.) exhibit strong positive gravitropism. In both species, gravistimulation induces polar movement of calcium across the root tip from the upper side to the lower side. Roots of onion (Allium cepa L.) are not responsive to gravity and gravistimulation induces little or no polar movement of calcium across the root tip. Treatment of maize or pea roots with inhibitors of auxin transport (morphactin, naphthylphthalamic acid, 2,3,5-triiodobenzoic acid) prevents both gravitropism and gravity-induced polar movement of calcium across the root tip. The results indicate that calcium movement and auxin movement are closely linked in roots and that gravity-induced redistribution of calcium across the root cap may play an important role in the development of gravitropic curvature.Abbreviations 9-HFCA 9-hydroxyfluorenecarboxylic acid - NPA naphthylphthalamic acid - TIBA 2,3,5-triiodobenzoic acid - IAA indole-3-acetic acid  相似文献   

7.
Exogenously applied brassinolide (BL) increased both gravitropic curvature and length of primary roots of Arabidopsis at low concentration (10(-10) M), whereas at higher concentration, BL further increased gravitropic curvature while it inhibited primary root growth. BRI1-GFP plants possessing a high steady-state expression level of a brassinosteroid (BR) receptor kinase rendered the plant's responses to gravity and root growth more sensitive, while BR-insensitive mutants, bri1-301 and bak1, delayed root growth and reduced their response to the gravitropic stimulus. The stimulatory effect of BL on the root gravitropic curvature was also enhanced in auxin transport mutants, aux1-7 and pin2, relative to wild-type plants, and increasing concentration of auxin attenuated BL-induced root sensitivity to gravity. Interestingly, IAA treatment to the roots of bri1-301 and bak1 plants or of plants pretreated with a BL biosynthetic inhibitor, brassinazole, increased their sensitivity to gravity, while these treatments for the BL-hypersensitive transgenic plants, BRI1-GFP and 35S-BAK1, were less effective. Expression of a CYP79B2 gene, encoding an IAA biosynthetic enzyme, was suppressed in BL-hypersensitive plant types and enhanced in BL-insensitive or -deficient plants. In conclusion, our results indicate that BL interacts negatively with IAA in the regulation of plant gravitropic response and root growth, and its regulation is achieved partly by modulating biosynthetic pathways of the counterpart hormone.  相似文献   

8.
Abstract: Gravitropism of the protonemata of the moss Ceratodon purpureus (Hedw.) Brid. was studied after treatment with auxin transport inhibitors and auxin-related substances. The phytotropins NPA (naphthylphthalamic acid) and PBA (pyrenoylbenzoic acid), known to block auxin efflux in higher plants, strongly inhibited gravitropic curvature of the apical protonemal cell. At 3 μM NPA or PBA, approximately 60 % inhibition of curvature was observed; growth rates were less affected. Tyrphostin A47, a known antagonist of NPA effects in higher plants, released the inhibition of moss protonemal gravitropism and restored the full curvature response. Exogenous IAA, even at high concentrations (40 μM), did not interfere with protonemal gravitropism. To account for the results, modified hypotheses for auxin transport and action are discussed.  相似文献   

9.
Tomato (Lycopersicon esculentum, Mill.) roots were analyzed during growth on agar plates. Growth of these roots was inhibited by the auxin transport inhibitors naphthylphthalamic acid (NPA) and semicarbazone derivative I (SCB-1). The effect of auxin transport inhibitors on root gravitropism was analyzed by measurement of the angle of gravitropic curvature after the roots were reoriented 90 degrees from the vertical. NPA and SCB-1 abolished both the response of these roots to gravity and the formation of lateral roots, with SCB-1 being the more effective at inhibition. Auxins also inhibited root growth. Both auxins tested has a slight effect on the gravity response, but this effect is probably indirect, since auxins reduced the growth rate. Auxins also stimulated lateral root growth at concentration where primary root growth was inhibited. When roots were treated with both IAA and NPA simultaneously, a cumulative inhibition of root growth was found. When both compounds were applied together, analysis of gravitropism and lateral root formation indicated that the dominant effect was exerted by auxin transport inhibitors. Together, these data suggest a model for the role of auxin transport in controlling both primary and lateral root growth.  相似文献   

10.
Buer CS  Muday GK 《The Plant cell》2004,16(5):1191-1205
We examined whether flavonoids act as endogenous auxin transport regulators during gravity vector and light intensity changes in Arabidopsis thaliana roots. Flavonoid deficient transparent testa4 [tt4(2YY6)] seedlings had elevated root basipetal auxin transport compared with the wild type, consistent with the absence of a negative auxin transport regulator. The tt4(2YY6) roots had delayed gravitropism that was chemically complemented with a flavonoid intermediate. Flavonoid accumulation was found in wild-type columella cells, the site of gravity perception, and in epidermal and cortical cells, the site of differential growth, but flavonoid accumulation was absent in tt4(2YY6) roots. Flavonoid accumulation was higher in gravity-stimulated root tips as compared with vertical controls, with maximum differences coinciding with the timing of gravitropic bending, and was located in epidermal cells. Exogenous indole-3-acetic acid (IAA) also elevated flavonoid accumulation, suggesting that flavonoid changes in response to gravity might be partly as a result of changing IAA distribution. Acropetal IAA transport was also elevated in roots of tt4(2YY6). Flavonoid synthesis was repressed in the dark, as were differences in root acropetal transport in tt4(2YY6). These results are consistent with light- and gravity-induced flavonoid stimulation that alters auxin transport in roots and dependent physiological processes, including gravitropic bending and root development.  相似文献   

11.
12.
We have found that chromosaponin I (CSI), a gamma-pyronyl-triterpenoid saponin isolated from pea (Pisum sativum L. cv Alaska), specifically interacts with AUX1 protein in regulating the gravitropic response of Arabidopsis roots. Application of 60 microM CSI disrupts the vertically oriented elongation of wild-type roots grown on agar plates but orients the elongation of agravitropic mutant aux1-7 roots toward the gravity. The CSI-induced restoration of gravitropic response in aux1-7 roots was not observed in other agravitropic mutants, axr2 and eir1-1. Because the aux1-7 mutant is reduced in sensitivity to auxin and ethylene, we examined the effects of CSI on another auxin-resistant mutant, axr1-3, and ethylene-insensitive mutant ein2-1. In aux1-7 roots, CSI stimulated the uptake of [(3)H]indole-3-acetic acid (IAA) and induced gravitropic bending. In contrast, in wild-type, axr1-3, and ein2-1 roots, CSI slowed down the rates of gravitropic bending and inhibited IAA uptake. In the null allele of aux1, aux1-22, the agravitropic nature of the roots and IAA uptake were not affected by CSI. This close correlation between auxin uptake and gravitropic bending suggests that CSI may regulate gravitropic response by inhibiting or stimulating the uptake of endogenous auxin in root cells. CSI exhibits selective influence toward IAA versus 1-naphthaleneacetic acid as to auxin-induced inhibition in root growth and auxin uptake. The selective action of CSI toward IAA along with the complete insensitivity of the null mutant aux1-22 toward CSI strongly suggest that CSI specifically interacts with AUX1 protein.  相似文献   

13.
14.
Gravity plays a fundamental role in plant growth and development, yet the molecular details of gravitropism is not fully understood. Here, we report the effects of PD98059, a specific inhibitor of mitogen-activated protein (MAP) kinase kinase, on the gravitropism of primary roots of maize. Unilateral application of PD98059 to horizontal roots led to different gravitropic growth. Placing PD98059-containing agar on the upper side of the root tips accelerated gravitropic curvature, whereas placing the agar on the lower side inhibited gravitropic curvature. However, no effect was detected when asymmetric application of PD98059 to vertical roots. Global application of maize primary root with PD98059 suppressed root gravitropism. Furthermore, the effects of H2O2 on horizontal root gravitropism and vertical root bending were compromised by pretreatment with PD98059. These results suggest an involvement of MAP kinase pathway(s) in gravitropism of maize roots.  相似文献   

15.
16.
Using an auxanometer and time-lapse cinematography we have studied the timing of abscisic acid (ABA) effects on elongation, gravitropic curvature, and hydrogen-ion efflux in several cultivars of maize (Zea mays L.). The effect of high concentrations (e.g. 0.1 mM) of ABA on root elongation is triphasic, including 1) a period of promotion lasting approximately 12 h, 2) a subsequent period of increasing inhibition lasting approximately 12h, and 3) gradual recovery to a rate within approximately 80% of the control rate. With lower concentrations of ABA (e.g. 0.1 μM) only the transient promotive phase is seen. Abscisic acid enhances ethylene biosynthesis in roots of maize but suppression of ethylene biosynthesis does not prevent the long-term inhibitory action of ABA on growth. Application of ABA (0.1 mM) to the upper surface of horizontally placed roots accelerates positive gravitropism. Application of ABA to the lower surface retards gravitropism and in some cases causes the roots to curve upward against the direction of gravity. These observations are consistent with our finding that the initial effect of ABA on root elongation is stimulatory. Since root gravitropism is rapid enough to be completed within the stimulatory phase of ABA action, the data argue against hypotheses of gravitropism based upon accumulation of ABA to inhibitory levels on the lower side of a hirizontal root.  相似文献   

17.
Reversible protein phosphorylation is a key regulatory mechanism governing polar auxin transport. We characterized the auxin transport and gravitropic phenotypes of the pinoid-9 (pid-9) mutant of Arabidopsis (Arabidopsis thaliana) and tested the hypothesis that phosphorylation mediated by PID kinase and dephosphorylation regulated by the ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1 (RCN1) protein might antagonistically regulate root auxin transport and gravity response. Basipetal indole-3-acetic acid transport and gravitropism are reduced in pid-9 seedlings, while acropetal transport and lateral root development are unchanged. Treatment of wild-type seedlings with the protein kinase inhibitor staurosporine phenocopies the reduced auxin transport and gravity response of pid-9, while pid-9 is resistant to inhibition by staurosporine. Staurosporine and the phosphatase inhibitor, cantharidin, delay the asymmetric expression of DR5∷revGFP (green fluorescent protein) at the root tip after gravistimulation. Gravity response defects of rcn1 and pid-9 are partially rescued by treatment with staurosporine and cantharidin, respectively. The pid-9 rcn1 double mutant has a more rapid gravitropic response than rcn1. These data are consistent with a reciprocal regulation of gravitropism by RCN1 and PID. Furthermore, the effect of staurosporine is lost in pinformed2 (pin2). Our data suggest that reduced PID kinase function inhibits gravitropism and basipetal indole-3-acetic acid transport. However, in contrast to PID overexpression studies, we observed wild-type asymmetric membrane distribution of the PIN2 protein in both pid-9 and wild-type root tips, although PIN2 accumulates in endomembrane structures in pid-9 roots. Similarly, staurosporine-treated plants expressing a PIN2GFP fusion exhibit endomembrane accumulation of PIN2∷GFP, but no changes in membrane asymmetries were detected. Our data suggest that PID plays a limited role in root development; loss of PID activity alters auxin transport and gravitropism without causing an obvious change in cellular polarity.A variety of important growth and developmental processes, including gravity response, embryo and vascular development, and the branching of roots and shoots, are controlled by the directional and regulated transport of auxin in higher plants. Reversible protein phosphorylation is an important regulatory strategy that may modulate auxin transport and dependent processes such as root gravitropism, perhaps through action of the PINOID (PID) kinase (for review, see DeLong et al., 2002; Galvan-Ampudia and Offringa, 2007). PID is an AGC family Ser/Thr kinase (Christensen et al., 2000) and belongs to an AGC kinase clade containing WAG1, WAG2, AGC3-4, and D6PK/AGC1-1 (Santner and Watson, 2006; Galvan-Ampudia and Offringa, 2007; Zourelidou et al., 2009). PID activity has been demonstrated in vitro and in vivo (Christensen et al., 2000; Michniewicz et al., 2007), and several pid mutant alleles exhibit altered auxin transport in the inflorescence and a floral development defect resembling that of auxin transport mutants (Bennett et al., 1995). Overexpression of the PID gene results in profound alterations in root development and responses to auxin transport inhibitors, reduced gravitropism and auxin accumulation at the root tip (Christensen et al., 2000; Benjamins et al., 2001; Michniewicz et al., 2007), as well as enhanced indole-3-acetic acid (IAA) efflux in tobacco (Nicotiana tabacum) cell cultures (Lee and Cho, 2006) and altered PINFORMED1 (PIN1), PIN2, and PIN4 localization patterns (Friml et al., 2004; Michniewicz et al., 2007), consistent with PID being a positive regulator of IAA efflux. However, the effects of pid loss-of-function mutations on auxin transport activities and gravitropic responses in roots have not yet been reported (Robert and Offringa, 2008).In contrast, auxin transport and gravitropism defects of a mutant with reduced protein phosphatase activity have been characterized in detail. The roots curl in naphthylphthalamic acid1 (rcn1) mutation, which ablates the function of a protein phosphatase 2A regulatory subunit, causes reduced PP2A activity in vivo and in vitro (Deruère et al., 1999). Roots and hypocotyls of rcn1 seedlings have elevated basipetal auxin transport (Deruère et al., 1999; Rashotte et al., 2001; Muday et al., 2006), and rcn1 roots exhibit a significant delay in gravitropism, consistent with altered auxin transport (Rashotte et al., 2001; Shin et al., 2005). These data indicate that PP2A is a negative regulator of basipetal transport and suggest that if PID-dependent phosphorylation regulates root auxin transport and gravitropism, then it may act in opposition to PP2A-dependent dephosphorylation.In roots, auxin transport is complex, with distinct sets of influx and efflux carriers that define tissue-specific and opposing directional polarities (for review, see Leyser, 2006). IAA moves acropetally, from the shoot toward the root apex, through the central cylinder (Tsurumi and Ohwaki, 1978), and basipetally, from the root apex toward the base, through the outer layer of cells (for review, see Muday and DeLong, 2001). When plants are reoriented relative to the gravity vector, auxin becomes asymmetrically distributed across the root tip, as a result of a process termed lateral auxin transport (for review, see Muday and Rahman, 2008). Several carriers that mediate root basipetal IAA transport have been clearly defined and include the influx carrier AUXIN-INSENSITIVE1 (AUX1; Marchant et al., 1999; Swarup et al., 2004; Yang et al., 2006) and efflux carriers of two classes, PIN2 (Chen et al., 1998; Müller et al., 1998; Rashotte et al., 2000) and ATP-BINDING CASSETTE TYPE B TRANSPORTER4/MULTIDRUG-RESISTANT4/P-GLYCOPROTEIN4 (ABCB4/MDR4/PGP4; Geisler et al., 2005; Terasaka et al., 2005; Lewis et al., 2007). Lateral transport at the root tip may be mediated by PIN3, an efflux carrier with a gravity-dependent localization pattern (Friml et al., 2002; Harrison and Masson, 2007).Gravitropic curvature of Arabidopsis (Arabidopsis thaliana) roots requires changes in IAA transport at the root tip (for review, see Muday and Rahman, 2008). Auxin transport inhibitors (Rashotte et al., 2000) and mutations in genes encoding basipetal transporters, including aux1 (Bennett et al., 1996), pin2/agr1 (Chen et al., 1998; Müller et al., 1998), and abcb4/mdr4/pgp4 (Lin and Wang, 2005; Lewis et al., 2007), alter gravitropism. Auxin-inducible reporters exhibit asymmetric expression across the root tip prior to differential growth, and this asymmetry is abolished by treatment with auxin transport inhibitors that prevent gravitropic curvature (Rashotte et al., 2001; Ottenschläger et al., 2003). Additionally, the pin3 mutant exhibits slightly reduced rates of gravitropic curvature (Harrison and Masson, 2007), and PIN3 is expressed in the columella cells, which are the site of gravity perception (Blancaflor et al., 1998; Friml et al., 2002). The PIN3 protein relocates to membranes on the lower side of columella cells after gravitropic reorientation, consistent with a role in facilitating asymmetric IAA transport at the root tip (Friml et al., 2002; Harrison and Masson, 2007).The available data suggest a model in which PID and RCN1 antagonistically regulate basipetal transport and gravitropic response in root tips (Fig. 1). In this model, the regions with the highest IAA concentrations in the epidermal and cortical cell layers are indicated by shading, and the arrows indicate the direction and relative amounts of basipetal auxin transport. Our previous work suggests that elevated basipetal IAA transport in rcn1 roots impairs gravitropic response, presumably due to the inability of roots either to form or to perceive a lateral auxin gradient in the context of a stronger polar IAA transport stream (Rashotte et al., 2001). Enhanced basipetal transport may increase the initial auxin concentration along the upper side of the root, impeding the establishment or perception of a gradient in rcn1 and cantharidin-treated wild-type roots (Fig. 1, right). Based on the published pid inflorescence transport data (Bennett et al., 1995), we hypothesize that pid seedling roots and staurosporine-treated wild-type roots have reduced basipetal auxin transport (Fig. 1, left). Upon reorientation of roots relative to the gravity vector, the reduced basipetal IAA transport in pid may lead to slower establishment of an auxin gradient across the root. This model then predicts that cantharidin treatment of pid-9 or staurosporine treatment of rcn1 seedlings would enhance or restore gravitropism in these mutants. Similarly, a double mutant might be expected to exhibit a corrected gravitropic response relative to the single mutants.Open in a separate windowFigure 1.Auxin transport defects in pid-9 and rcn1 mutants alter auxin redistribution after reorientation relative to the gravity vector. This model predicts that differences in basipetal auxin transport activities of wild-type, pid-9, and rcn1 roots will affect the formation of lateral auxin gradients. The shaded area in each root represents the region of highest IAA concentration in epidermal and cortical cells, with darker shading in the central columella cells, believed to be the auxin maxima. The direction and amount of basipetal IAA transport are indicated by arrows. The region of differential growth during gravitropic bending is indicated by the shaded rectangle. If auxin transport is reduced (as shown in the pid-9 mutant or in staurosporine-treated seedlings), this would lead to a slower formation of an auxin gradient in root tips. The rcn1 mutation (or treatment with cantharidin) has already been shown to lead to increased basipetal transport and a reduced rate of gravitropic bending, consistent with altered formation or perception of an auxin gradient. The antagonistic effects of kinase and phosphatase inhibition are predicted to lead to normal gravity responses in the pid-9 rcn1 double mutant as well as in pid-9 and rcn1 single mutants treated with the “reciprocal” inhibitor.The experiments described here were designed to test this model by examining gravitropism and root basipetal IAA transport in pid and staurosporine-treated seedlings. We investigated the regulation of gravity response by PID kinase and RCN1-dependent PP2A activities and observed antagonistic interactions between the rcn1 and pid-9 loss-of-function phenotypes that are consistent with reciprocal kinase/phosphatase regulation. We found that loss of kinase activity in the pid mutant and in staurosporine-treated wild-type plants inhibits basipetal auxin transport and the dependent physiological process of root gravitropism. Our results suggest that staurosporine acts to regulate these processes through inhibition of PID kinase and that PID effects are PIN2 dependent. In both wild-type and pid-9 roots, we observed polar membrane distribution of the PIN2 protein; unlike wild-type roots, though, pid-9 roots exhibited modest accumulation of PIN2 in endomembrane structures. Similarly, we detected asymmetric distribution and endomembrane accumulation of PIN2∷GFP in staurosporine-treated roots. Our data suggest that PID plays a limited role in root development; loss of PID activity alters PIN2 trafficking, auxin transport, and gravitropism without causing an obvious loss of cellular polarity. Together, these experiments provide insight into phosphorylation-mediated control of the gravity response and auxin transport in Arabidopsis roots.  相似文献   

18.
We examined the influence of aluminum and calcium (and certain other cations) on hormone transport in corn roots. When aluminum was applied unilaterally to the caps of 15 mm apical root sections the roots curved strongly away from the aluminum. When aluminum was applied unilaterally to the cap and 3H-indole-3-acetic acid was applied to the basal cut surface twice as much radioactivity (assumed to be IAA) accumulated on the concave side of the curved root as on the convex side. Auxin transport in the apical region of intact roots was preferentially basipetal, with a polarity (basipetal transport divided by acropetal transport) of 6.3. In decapped 5 mm apical root segments, auxin transport was acropetally polar (polarity = 0.63). Application of aluminum to the root cap strongly promoted acropetal transport of auxin reducing polarity from 6.3 to 2.1. Application of calcium to the root cap enhanced basipetal movement of auxin, increasing polarity from 6.3 to 7.6. Application of the calcium chelator, ethylene-glycol-bis-(β-aminoethylether)-N,N,N′, N′-tetraacetic acid, greatly decreased basipetal auxin movement, reducing polarity from 6.3 to 3.7. Transport of label after application of tritiated abscisic acid showed no polarity and was not affected by calcium or aluminum. The results indicate that the root cap is particularly important in maintaining basipetal polarity of auxin transport in primary roots of corn. The induction of root curvature by unilateral application of aluminum or calcium to root caps is likely to result from localized effects of these ions on auxin transport. The findings are discussed relative to the possible role of calcium redistribution in the gravitropic curvature of roots and the possibility of calmodulin involvement in the action of calcium and aluminum on auxin transport.  相似文献   

19.
Root tip is capable of sensing and adjusting its growth direction in response to gravity, a phenomenon known as root gravitropism. Previously, we have shown that negative gravitropic response of roots (NGR) is essential for the positive gravitropic response of roots. Here, we show that NGR, a plasma membrane protein specifically expressed in root columella and lateral root cap cells, controls the positive root gravitropic response by regulating auxin efflux carrier localization in columella cells and the direction of lateral auxin flow in response to gravity. Pharmacological and genetic studies show that the negative root gravitropic response of the ngr mutants depends on polar auxin transport in the root elongation zone. Cell biology studies further demonstrate that polar localization of the auxin efflux carrier PIN3 in root columella cells and asymmetric lateral auxin flow in the root tip in response to gravistimulation is reversed in the atngr1;2;3 triple mutant. Furthermore, simultaneous mutations of three PIN genes expressed in root columella cells impaired the negative root gravitropic response of the atngr1;2;3 triple mutant. Our work revealed a critical role of NGR in root gravitropic response and provided an insight of the early events and molecular basis of the positive root gravitropism.  相似文献   

20.
Artemisinin, an antimalarial secondary metabolite produced in Artemisia species, also has been recognized as an allelochemical that inhibits the growth of several plant species. However, the phytotoxicity mechanism of artemisinin is not exhaustively deciphered up to now. In this research, the effects of artemisinin on Arabidopsis thaliana root gravitropic curvature and development were characterized. Exogenously applied artemisinin disturb the root gravitropic responses, inhibited the elongation of primary and lateral roots and root hairs in a concentration-dependent fashion, and prevented the formation of lateral roots and root hairs. Moreover, the number of starch grain and the distribution range of auxin in the root tip was reduced by artemisinin, and the redistribution of auxin was less sensitive to gravity stimulus when treated with artemisinin than that of control. The expression of auxin transporter PIN2 was partially suppressed by artemisinin. Together, the results demonstrated that the effects of artemisinin on root gravitropism and root system development were largely dependent on the reduction of starch grain and auxin levels, as well as the disordered lateral auxin redistribution.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号