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1.
Functional response of a solitary, larval-pupal endoparasitoid of Liriomyza leafminers, Ganaspidium utilis Beardsley, was estimated on Liriomyza trifolii Burgess at three temperatures (17℃, 25℃, 29℃ ) and host densities. A type Ⅱ random parasitoid equation (RPE) was used to estimate instantaneous search rate and handling time. The instantaneous search rate increased as temperature increased. All of the RPE regressions obtained for functional response of G. utilis at different temperatures were significant (P〈0.01). The slope of RPE regression lines was lower across the temperatures. At 29±2℃, the maximum number of larvae parasitized was 7.8 per day. It decreased to 7.2 larvae parasitized at 25±2℃. At 17±2℃, no significant increment of parasitization was observed due to the host density increments. The estimated handling time was lowest at 17±2℃ and highest at 25 ± 2℃, respectively. The ability of G. utilis to find and parasitize L. trifolii over a wide range of temperatures makes them a good candidate for biological control of Liriomyza leafminers.  相似文献   

2.
Venom and ovarian proteins in braconid and ichneumonid wasps play an important role in the successful parasitism of their host, especially for immune suppression immediately after oviposition. In this study, we compared the effect of venom and ovarian proteins collected from the female wasps of Macrocentrus cingulum, a polyembryonic parasitoid of the larvae of Ostriniafunacalis, on the encapsulation capacity of Sephadex A- 25 beads at 4 h and 24 h post-injection both in vivo and in vitro. The results showed that the ovarian proteins significantly interfered with the encapsulation capacity of hemocytes in a dose-dependent manner. Spreading and viability of hemocytes in O. furnacalis was not disrupted by venom and ovarian proteins at various concentrations injected. It seems likely that the ovarian proteins from M. cingulum play a role in suppressing the encapsulation capacity of host hemocytes.  相似文献   

3.
Superparasitism in solitary parasitoids results in fatal competition between the immature parasitoids, and consequently only one individual can emerge. In the semisoli- tary ovicidal parasitoid Echthrodelphaxfairchildii (Hymenoptera: Dryinidae), 2 adults can emerge under superparasitism with a short interval (〈24 h) between the first and second ovipositions. We determined the female parasitoid's behavioral responses under self- and conspecific superparasitism bouts with first-to-second oviposition intervals of 〈2 h. The self- and conspecific superparasitizing frequencies increased up to an oviposition interval of 0.75 h, with the former remaining lower than the latter, particularly for oviposition intervals of _〈0.25 h, suggesting the existence of self-/conspecific discrimination. The superparasitizing frequency plateaued for oviposition intervals of _〉0.75 h, with no dif- ference between self- and conspecific superparasitism. The ovicidal-probing frequency did not differ under self- and conspecific superparasitism, and was usually 〈20%. The females exhibited no preference for the oviposition side (i.e., ovipositing on the side with or without the first progeny) and almost always laid female eggs for any oviposition in- terval under self- and conspecific superparasitism. The sex ratio was not affected by the type of superparasitism, oviposition sides, or the occurrence of ovicidal probing. These observed results about the oviposition side, ovicidal probing, and sex ratios differed from the predictions obtained assuming that the females behave optimally. Possible reasons for the discrepancies are discussed: likely candidates include the high cost of selecting oviposition sides and ovicidal probing, and, for the sex ratio, the low frequency of encountering suitable hosts before superparasitism bouts.  相似文献   

4.
Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoidHabrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full-length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70IIl) from 11. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple se- quence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real-time quantitative poly- merase chain reaction analysis showed that the expression of the three HhHspTO genes in H. hebetor reared at different conditions was quite different. HhHspTOI showed higher relative expression when H. hebetor were reared at 27.5℃ than at two lower temperatures (17.5℃ and 20℃) regardless of the photoperiod, whereas HhHspTOII showed higher ex- pression when H. hebetor were reared at 20℃ and 10 : 14 L : D than when reared at 17.5℃ and either 16 : 8 L : D or 10 : 14 L : D. In contrast, HhHSP7OIIIwas expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHspTO genes in H. hebetor.  相似文献   

5.
A female-specific protein, vitellogenin (Vg), and its corresponding egg vitellin (Vt) are identified in the ectoparasitic wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt. Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20% sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production. Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20% sucrose.  相似文献   

6.
Abstract Field doses of six selected insecticides were tested against the immature (pupae) and mature (adult) stages of Diadegma semiclausum (Hellén) and Oomyzus sokolowskii (Kurdjumov), parasitoids of the diamondback moth, Plutella xylostella (L.). Effects of contact toxicity (direct spraying) of the six insecticides on emergence of parasitoids were found negligible on both species except permethrin which caused 37.5% mortality. All adults of both parasitoid species died 24 hours after exposure to chlorfenapyr, emamectin benzoate and permethrin. In contrast, the three insect growth regulators (IGRs), chlorfluazuron, flufenoxuron and teflubenzuron, were found harmless to both species, and adult mortality of both parasitoid species was 0–16.7%. However, parasitism by the females of both parasitoid species was severely impaired when the females were offered the three IGR diluted solutions for 24 hours. Effects of oral toxicities of the IGRs on longevity of both parasitoids after 12 hours exposure were found to be significantly different between males and females. Compatibility of tested insecticides with D. semiclausum and O. sokolowskii and integration of compatible insecticides with these parasitoids in integrated pest management programs of crucifers are discussed.  相似文献   

7.
We assessed the adaptiveness of refraining from infanticidal probing at the third attack under triple parasitism in the parasitoid Echthrodelphaxfairchildii Perkins, by comparing fitness returns from the third attack with and without probing. Fitness returns were assessed in terms of the survival rate, mean developmental time, and mean adult head width of the progenies. Not probing was maladaptive under triple parasitism with oviposition intervals of 1/24 and 24/24 hours (where the numbers before and after the slash refer to the first-to-second and second-to-third oviposition intervals, respectively), although no significant difference was detected in fitness returns for oviposition intervals of 24/1 hours. We suggest that the cost of probing (especially the decrease in the chance of future ovipositions) is a reason for the seemingly maladaptive absence of probing.  相似文献   

8.
Partial DNA sequences of three genes, that is, mitochondrial large ribosomal subunit (16S), nuclear large ribosomal subunit (28S D2) and mitochondrial NADH1 dehydrogenase (NADH1) gene, were sequenced from different microgas trine species(Braconidae: Microgastrinae) collected fresh from paddy fields. The DNA sequences were used to determine the extent of sequence variation among species in order to evaluate the specific status of each species. Cladistic analysis was also used to infer a phylogenetic relationship among these species. The results showed that sequence divergence among species of the same genus Cotesia was much lower than those among different genera, such as Cotesia, Exoryza and Apanteles; the sequence similarity of 16S rDNA and NADH 1 genes between Cotesia sp. and C. chilonis was higher than that between C. sp. and C. ruficrus.Phylogenetic analyses suggested that four species of Cotesia were always grouped in the same clade regardless of using different analysis methods; Cotesia sp. and C. chilonis are more closely related to each other than to C. ruficrus, different from previous morphological results. Additionally, sequence analyses indicated that NADH1 gene has more parsimony informative characters than 28S rDNA D2 and 16S rDNA at the species-level analysis,indicating that NADH1 gene might be a useful marker for species-level analysis.  相似文献   

9.
Intraguild predation (IGP) can be an important factor influencing the effective- ness of aphid natural enemies in biological control. In particular, aphid parasitoid foraging could be influenced by the presence of predators. This study investigated the effect of larvae of the predatory hoverfly Episyrphus balteatus DeGeer (Diptera: Syrphidae) and the multicolored Asian ladybird Harmonia axyridis Pallas (Coleoptera: Coccinellidae) on the foraging behavior of the aphid parasitoid, Aphidius ervi Haliday (Hymenoptera: Aphidiidae) in choice experiments using a leaf disc bioassay. Wasp response to chemical tracks left by those predator larvae was also tested. Parasitoid behavior was recorded using the Observer (Noldus Information Technology, version 5.0, Wageningen, the Netherlands). The experiments were conducted under controlled environmental conditions using leaves of the broad bean plant, Viciafaba L. (Fabaceae) with Myzus persicae Sulzer (Homoptera: Aphididae) as the host complex. A. ervi females avoided aphid patches when larvae of either predator were present. A similar avoidance response was shown by A. ervi to aphid patches with E. balteatus larval tracks, whereas no significant response was observed to tracks left by H. axyridis larvae. It was concluded that IG predator avoidance shown by the aphid parasitoid A. ervi may be a factor affecting their distribution among host patches.  相似文献   

10.
Pheidole oxyops builds subterranean nests, with an external architecture that is distinctive and easily recognizable by its wide and specific entrance hole, measuring up to 12.2 cm in diameter, denoting a pitfall-trap. In order to study the nests' internal architecture, seven nests were excavated; four were identified with neutral talc, while the others were cast in cement and then excavated. Measurements were made in order to gain a better understand- ing of their structures, and a photographic documentation was obtained as well. The excavations revealed that the nests are perpendicular relative to the ground, beginning with a cylindrical channel with a mean length of 13.5 cm, containing irregular formations, and whose diameter becomes progressively narrower until the first chamber is formed. As the channel continues, dish-like chambers appear, interconnected by channels that become progressively narrower and longer, while the chambers are arranged at greater distances from each other as nest depth increases. Both channels and chambers are located on the vertical projection of the entrance hole. Nests may reach a depth of up to 5.09 m, with a number of chambers ranging between 4 and 14.  相似文献   

11.
寄主抗药性对菜蛾绒茧蜂生物学特性的影响   总被引:2,自引:1,他引:1       下载免费PDF全文
以小菜蛾Plutella xylostella敏感品系SP作寄主饲育的菜蛾绒茧蜂Cotesia plutellae SC品系分别寄生于小菜蛾的SP品系(SC-SP组合)或抗性RP品系(SC-RP组合),还以小菜蛾RP品系作寄主饲育的菜蛾绒茧蜂RC品系分别寄生于小菜蛾的SP品系(RC-SP组合)或RP品系(RC-RP组合),均在幼虫中期施用氰戊菊酯,考察了药剂对该蜂生物学特性的影响。结果发现:在不施用杀虫剂时,SC-RP组合中蜂的结茧率为45.8%,显著低于其它组合, 所结茧长0.76 mm,育出雌蜂前翅和后足胫节长分别为3.28 mm和2.33 mm,也分别小于其它各组合的结果,表明寄主抗药性对该蜂有不利影响;施用杀虫剂后,RC-RP、SC-RP组合中,蜂的结茧率分别为95.5%和37.8%,显著高于SC-SP和RC-SP组合中蜂的结茧率(22.5%,25.8%),表明寄主抗性能保护其体内的幼蜂少受杀虫剂的影响;RC-SP组合在受到和未受到杀虫剂作用时茧的羽化率分别为95.2%和93.6%,无显著差异,卵+幼虫及雌蛹的发育历期在处理和对照间也无显著差异,表明用抗性寄主饲育的菜蛾绒茧蜂在寄生敏感寄主时仍表现一定的耐药性,有利于该蜂抗药性的发展,即寄主 寄生蜂之间在抗药性方面存在协同进化。  相似文献   

12.
寄主抗药性对菜蛾绒茧蜂抗药性发展的影响   总被引:1,自引:4,他引:1       下载免费PDF全文
室内筛选小菜蛾中抗品系(SRP)、高抗品系(RP)、以敏感小菜蛾幼虫(SP)为寄主的菜蛾绒茧蜂SRC品系和以SRP幼虫为寄主的菜蛾绒茧蜂RSC品系对氰戊菊酯的抗性。分别筛选了13、14、14和13代。小菜蛾SRP和RP品系分别获得了68.9和605.8倍的抗性,菜蛾绒茧蜂SRC和RSC品系分别获得了4.3和11.0倍的抗性。上述结果表明通过施药于体内有寄生蜂的小菜蛾幼虫筛选寄生蜂抗性,可以获得具有抗性的寄生蜂。以SRP为寄主的RSC品系的抗性水平高于以SP为寄主的SRC品系的抗性水平,表明和抗性较高的寄主同步筛选,寄生蜂的抗性发展更快。小菜蛾SP、SRP和RP三个品系幼虫的多功能氧化酶(MFO)活性比为1∶1.15∶1.50;菜蛾绒茧蜂SC、SRC和RSC三个品系幼虫的MFO活性比为1∶1.10∶1.49,成蜂的MFO活性比为1∶1.18∶1.54;而每种昆虫不同品系的羧酸酯酶(CarE)、总酯酶(Es)活性水平与其抗性水平变化不一致,表明抗性与MFO活性升高有关,而与CarE和Es的活性无关。  相似文献   

13.
甜菜夜蛾对氰戊菊酯和顺式氯氰菊酯的抗性机理   总被引:2,自引:0,他引:2  
通过对活体增效作用进行测定和生化分析,探讨了甜菜夜蛾对氰戊菊酯和顺式氯氰菊酯的抗性机理.结果表明:增效醚(PBO)、增效磷(SV1)、磷酸三苯酯(TPP)和顺丁烯二酸二乙酯(DEM)对甜菜夜蛾抗氰戊菊酯品系(Fen-R)和敏感品系(S)的增效倍数之比分别为10.2、7.8、12.5和1.1,对抗顺式氯氰菊酯品系(Cyp-R)和敏感品系(S)的增效倍数之比分别为21.6、15.5、8.6和1.2.PBO、SV1和TPP对氰戊菊酯和顺式氯氰菊酯均有显著增效作用,表明多功能氧化酶和羧酸酯酶均参与了甜菜夜蛾对氰戊菊酯和顺式氯氰菊酯的抗性.Fen-R品系和Cyp-R品系4龄幼虫羧酸酯酶的活性分别是S品系的1.9和2.2倍,而谷胱甘肽-S-转移酶活性与S品系差异不显著,表明羧酸酯酶活性的提高是甜菜夜蛾对氰戊菊酯和顺式氯氰菊酯产生抗性的重要原因,谷胱甘肽-S-转移酶与两种药剂的抗性无关.Fen-R品系和Cyp-R品系的Na-K-ATPase活性与S品系均无显著差异,但在相同浓度下氰戊菊酯和顺式氯氰菊酯对S品系Na-K-ATPase的抑制作用显著高于抗性品系,表明抗性品系Na-K-ATPase对杀虫剂的敏感性已明显降低.  相似文献   

14.
15.
棉铃虫对氰戊菊酯等杀虫剂抗性的选育及其生化机理   总被引:15,自引:1,他引:15       下载免费PDF全文
从用药水平低的棉田采集棉铃虫Helicover pa armigera(Hubner),在室内恒温条件下以人工饲料饲养,用氰戊菊酯等4种杀虫剂和1种混合剂经点滴法分别逐代处理棉铃虫幼虫,以选育其抗药性。用氰戊菊酯选择15代,抗性达311倍;而用灭多威、甲基对硫磷和辛硫磷分别选择13代、14代及13代,抗性仅分别达10.8倍、3.5倍及5.2倍,抗性发展较慢;甲基对硫磷与辛硫磷的混合剂选择12代,抗性也只有4.8倍。氰戊菊酯和溴氰菊酯、三氟氯氰菊酯间存在明显的交互抗性。用生测法测定酶抑制剂和生化法测定酶活性的结果表明,棉铃虫对氰戊菊酯的抗性主要与幼虫体内多功能氧化酶和羧酸酯酶的活性提高有关。  相似文献   

16.
17.
18.
在28℃下,以小菜蛾3龄幼虫作寄主,研究了菜蛾绒茧蜂与菜蛾啮小蜂间的相互关系.当寄主供2种蜂同时产卵寄生时,与只供1种蜂时相比。绒茧蜂的寄生率无显著变化,而啮小蜂的寄生率则显著下降;2种蜂的合计寄生率与任一种蜂单独存在时相比无显著差异.当寄主先供绒茧蜂寄生,再供啮小蜂寄生时,绒茧蜂的成功寄生率不受影响,而啮小蜂的寄生率仅为8%~13%;啮小蜂能寄生在寄主体内的绒茧蜂高龄幼虫.绒茧蜂能寄生已被啮小蜂寄生的寄主幼虫,其子代部分个体能正常发育至成虫羽化.当已被绒茧蜂寄生和未被寄生的寄主同时存在时,啮小蜂主要寄生未被寄生的寄主.表明绒茧蜂具有竞争优势。但这种优势可因啮小蜂的寄生而被削弱.  相似文献   

19.
Abstract  Resistance alleviation and mechanism in the 2nd instar larvae of one susceptible and two resistant strains of the cotton bollworm, Helicoverpa armigera to fenvalerate were studied at 24 h after the larvae were treated with Bacillus thuringiensis Berliner ( B.t. ) preparation. The results showed that responses of larvae with B. t pretreatment to fenvalerate were much more sensitive than those without B. t pretreatment. Compared with the susceptible strain (YZ-S), net ratios between LD50s with and without B. t pretreatment in the two resistant strains increased approximate 62. 6% and 80. 9%, respectively) inhibition of specific inhibitors to esterases displayed that efficacy of malaoxon and paraoxon methyl to acetyl-cholinesterase increased from 66.1% to 99. 8% in vitro and in vivo ; dynamic factors of acetylcholinesterase were significantly changed, in which K m and V max values decreased from 45. 58% to 68. 62% and K i values of malaoxon and paraoxon methyl to acethylcholinesterase increased approximate 60%. It suggested that the sensitivity of the 2nd instar larvae of resistant H. armigera to fenvalerate might increase after B. t pretreatment for 24 h, and change of acetylcholinesterase was an important factor during resistance alleviation by B. t pretreatment. The research showed that it is practicable to bring the coordinated use of B. t and chemical insecticides in IPM system.  相似文献   

20.
吴刚 《昆虫学报》2005,48(4):531-536
采用药膜法研究了福州建新和闽侯上街菜蚜茧蜂Diaeretiella rapae对杀虫剂抗药性的季节性变化。田间菜蚜茧蜂种群在网室内脱离选择压力后对杀虫剂敏感性显著升高。与敏感的F21子代的LC50相比,2002年6月8日至2004年11月18日期间,建新菜区菜蚜茧蜂对甲胺磷、氟虫腈、阿维菌素、氰戊菊酯和吡虫啉的抗性倍数分别为11.8~31.2,9.3~14.7,9.3~35.7,7.0~16.6,和13.3~30.1倍;2003年6月5日至2004年12月17日期间,上街菜区菜蚜茧蜂对甲胺磷、氟虫腈、阿维菌素、氰戊菊酯和吡虫啉的抗性倍数分别为7.5~34.9,4.0~14.3,9.2~38.6,7.8~18.9和12.1~24.2倍。田间菜蚜茧蜂种群在5月份和6月初抗性水平最高,12月份较低,6月末最低,尤其在6月份期间20天内抗性水平急剧下降。研究结果表明,田间菜蚜茧蜂对6种杀虫剂的抗性水平在脱离选择压力后不稳定,且呈显著的季节性变化。表2参25  相似文献   

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