共查询到20条相似文献,搜索用时 109 毫秒
1.
昆明小鼠4个可能近交系的基因分型验证 总被引:1,自引:0,他引:1
在我国,昆明小鼠作为一种实验动物广泛应用于药理和遗传学相关的研究领域。但由于昆明小鼠属于远交群,而且不同地区的种群间已经出现了严重分化,缺乏具有显著特征的近交系,这使得它在生物学上的应用受到了很大的限制。研究人员已经以昆明小鼠为背景培育出了几个可能的近交系,但由于缺乏可靠的遗传检测,至今未得到广泛的认可和应用。文章收集昆明小鼠的4个已经60代以上兄妹交配繁殖的可能近交系,并以两个标准近交系BALB/c和C57BL/6为参照,利用30个微卫星标记对每个品系的5只小鼠进行了微卫星基因分型,进而分析其遗传纯度。结果发现,品系A1和品系N4在本研究所用的30个位点均呈纯合状态;而T2和N2均在D15Mit16位点呈杂合状态。本研究第一次为我国昆明小鼠近交系的遗传学纯度提供了可靠的分子水平证据。今后应当加强昆明小鼠近交系的标准化,以扩大其在遗传学方面的应用。 相似文献
2.
应用RAPD方法对近交系小鼠进行遗传检测的研究 总被引:11,自引:1,他引:10
目的 为实验室日常检测近交系小鼠的遗传背景提供一种分子生物学方法。方法 用 6条随机引物对 6个品系近交系小鼠基因组DNA进行PCR扩增。结果 6条随机引物中p2、p3、p5和p6四引物扩增的条带差异较为明显。结论 RAPD方法是一种有效的近交系小鼠遗传检测手段 相似文献
3.
双色荧光杂交芯片在近交系小鼠遗传监测中的应用 总被引:2,自引:0,他引:2
应用一种新的高通量SNP检测方法-双色荧光杂交芯片技术进行近交系小鼠遗传监测。应用双色荧光杂交芯片技术对4个品系近交系小鼠的多个基因组DNA样本进行SNP分型,整合6个SNP位点的芯片杂交信息,对样本所属品系进行判断。研究结果表明SNP检测方法-双色荧光杂交芯片技术能够对选定的6个SNP位点进行高准确率分型;双色荧光杂交芯片技术是一种高通量SNP检测的良好工具,适合于对少量近交系品系来源的大样本量小鼠进行遗传污染监测和品系鉴定,并具有扩大应用的潜力。 相似文献
4.
应用一种新的高通量SNP检测方法-双色荧光杂交芯片技术进行近交系小鼠遗传监测。应用双色荧光杂交芯片技术对4个品系近交系小鼠的多个基因组DNA 样本进行SNP分型,整合6个SNP位点的芯片杂交信息,对样本所属品系进行判断。研究结果表明SNP检测方法-双色荧光杂交芯片技术能够对选定的6个SNP位点进行高准确率分型;双色荧光杂交芯片技术是一种高通量SNP检测的良好工具,适合于对少量近交系品系来源的大样本量小鼠进行遗传污染监测和品系鉴定,并具有扩大应用的潜力。 相似文献
5.
黑腹果蝇(Drosophila melanogaster)是进行生物医学研究的理想模型,但研究过程往往受到遗传工具和品系资源的限制,无法有效地调控目的基因表达,阻碍实验的深入开展。为了方便果蝇领域的实验室开展研究,我们首先开发并优化了基于CRISPR/Cas9的基因组编辑技术,随后研发了转录激活系统和新一代转基因干扰技术,最终可以简单高效、准确特异地调控目的基因表达。同时,利用这些遗传学新技术,我们构建了相应的基因敲除、转基因转录激活、转基因干扰的果蝇品系资源库,使清华大学果蝇中心成为世界上最重要的果蝇技术和资源中心之一。这些新技术以及相应的果蝇资源,正在被国内外果蝇研究领域的实验室所应用,对发育和疾病等相关研究发挥着广泛的促进作用。 相似文献
6.
单管双向等位基因专一性扩增方法在近交系小鼠遗传检测中的应用 总被引:1,自引:0,他引:1
目的将新近建立的单管双向等位基因专一性扩增(single-tube bi-directional allele specific amplification,SB-ASA)方法用于分析近交系小鼠基因组中的单核苷酸多态性(SNP)。方法以5个近交系小鼠为研究对象,采用SB-ASA方法对其16个SNP位点进行检测,并通过双盲实验和测序验证该方法的可靠性;且考察了该方法中PCR反应各成分及扩增条件对结果的影响。结果16个SNP位点,SB-ASA都成功地对5个品系小鼠进行了分型,与测序结果完全一致;双盲实验结果显示通过3个SNP位点即可鉴别5个品系。结论SB-ASA方法可用于近交系小鼠SNP的遗传检测,可望作为一种新的分子生物学遗传检测方法推广应用。 相似文献
7.
利用生化标记基因(biochemical markergene)对近交系小鼠的遗传纯度进行检测,目前已成为各国实验动物遗传监测的主要方法。随着大鼠在生物医学研究中应用的日益广泛,目前世界上育成的近交系大鼠数目已逾百个,我国近年来引进及自己育成的至少也有十几个品系。为了建立近交系大鼠遗传质量监测的生化方法,根据近年来国际上大鼠生化遗传学方面的进展,我们利用同工酶电泳方法,对Wistar等两个远交封闭群和ACI等两个近交系大鼠在九个生化位点的遗传多态性进行了检查,现报告如下: 相似文献
8.
9.
近交系小鼠遗传质量监测新方法的建立——染色体Q—H分带技术及其应用 总被引:3,自引:1,他引:2
本研究利用最新的Quinacrine Mustard and 33258 Hoechst(Q—H)复合荧光染色技术对10个品系的近交系小鼠的核型进行分析。在同一细胞内,按各号染色体着丝粒带大小排列、分组,建立该10个品系近交系小鼠特有的染色体着丝粒带核型,作为各品系小鼠遗传质量监测的染色体标记指标。本研究还对615小鼠品系的生化标汜检测与染色体标记检测的结果进行比较,同时比较了不同来源615小鼠的染色体标记,从而进一步阐明了该方法作为实验动物遗传监测方法之一与其他方法间的互补性及其自身特点。 相似文献
10.
克隆DNA的定向诱变 总被引:2,自引:0,他引:2
克隆DNA的定向诱变史桂荣(黑龙江省农科院原子能利用研究所,哈尔滨)基因诱变技术是遗传学研究中最重要的手段之一。随着分子遗传学的发展,人们开始按照自己的意愿有目的地在离体条件下创造基因突变,然后把它置入生物体基因组中,观察分析这些突变的表型效应。这种... 相似文献
11.
12.
13.
Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
14.
15.
16.
17.
RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
18.
JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
19.
肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
20.
H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献