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1.
RNA silencing was originally identified as an immune system targeted against transposons and viruses, but is now also recognized as a major regulatory process that affects all layers of host gene expression through the activities of various small RNA species. Recent work in plants and animals indicates that viruses not only suppress, but can also exploit, endogenous RNA silencing pathways to redirect host gene expression. There are also indications that cellular, as opposed to virus-derived small RNAs, might well constitute an unsuspected defense layer against foreign nucleic acids. This complex interplay has implications in the context of disease resistance and evolution of viral genomes. 相似文献
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Invasive nucleic acids such as transposons and viruses usually exhibit aberrant characteristics, e.g., unpaired DNA or abnormal double-stranded RNA. Organisms employ a variety of strategies to defend themselves by distinguishing self and nonself substances and disabling these invasive nucleic acids. Furthermore, they have developed ways to remember this exposure to invaders and transmit the experience to their descendants. The mechanism underlying this inheritance has remained elusive. Recent research has shed light on the initiation and maintenance of RNA-mediated inherited gene silencing. Small regulatory RNAs play a variety of crucial roles in organisms, including gene regulation, developmental timing, antiviral defense, and genome integrity, via a process termed as RNA interference (RNAi). Recent research has revealed that small RNAs and the RNAi machinery are engaged in establishing and promoting transgenerational gene silencing. Small RNAs direct the RNAi and chromatin modification machinery to the cognate nucleic acids to regulate gene expression and epigenetic alterations. Notably, these acquired small RNAs and epigenetic changes persist and are transmitted from parents to offspring for multiple generations. Thus, RNAi is a vital determinant of the inheritance of gene silencing and acts as a driving force of evolution. 相似文献
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RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms. It has been used to regulate gene expression
and development. In addition, RNA silencing serves as an important mechanism in plants’ defense against invasive nucleic acids,
such as viruses, transposons, and transgenes. As a counter-defense, most plants, and some animal viruses, encode RNA silencing
suppressors to interfere at one or several points of the silencing pathway. In this study, we showed that Pns12 of RGDV (Rice gall dwarf virus) exhibits silencing suppressor activity on the reporter green fluorescent protein in transgenic Nicotiana benthamiana line 16c. Pns12 of RGDV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA. Expression
of Pns12 also enhanced Potato virus X pathogenicity in N. benthamiana. Collectively, these results suggested that RGDV Pns12 functions as a virus suppressor of RNA silencing, which might target
an upstream step of dsRNA formation in the RNA silencing pathway. Furthermore, we showed that Pns12 is localized mainly in
the nucleus of N. benthamiana leaf cells. 相似文献
4.
RNA silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. In plants, it has been demonstrated that RNA silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation in the scions. This has been obtained mostly using transgenic plants, which may be a drawback in current agriculture. In the present study, we examined the dynamics of infection of a resistance-breaking strain of Tomato spotted wilt virus (RB-TSWV) through the graft between an old Apulian (southern Italy) tomato variety, denoted Sl-Ma, used as a rootstock and commercial tomato varieties used as scions. In tests with non-grafted plants, Sl-Ma showed resistance to the RB-TSWV infection as viral RNA accumulated at low levels and plants recovered from disease symptoms by 21 days post inoculation. The resistance trait was transmitted to the otherwise highly susceptible tomato genotypes grafted onto Sl-Ma. The results from the analysis of small RNAs hallmark genes involved in RNA silencing and virus-induced gene silencing suggest that RNA silencing is involved in the resistance showed by Sl-Ma against RB-TSWV and in scions grafted on this rootstock. The results from self-grafted susceptible tomato varieties suggest also that RNA silencing is enhanced by the graft itself. We can foresee interesting practical implications of the approach described in this paper. 相似文献
5.
Organisms employ a fascinating array of strategies to silence invasive nucleic acids such as transposons and viruses. Although evidence exists for several pathways that detect foreign sequences, including pathways that sense copy number, unpaired DNA, or aberrant RNA (e.g., dsRNA), in many cases, the mechanisms used to distinguish "self" from "nonself" nucleic acids remain mysterious. Here, we describe an RNA-induced epigenetic silencing pathway that permanently silences single-copy transgenes. We show that the Piwi Argonaute PRG-1 and its genomically encoded piRNA cofactors initiate permanent silencing, and maintenance depends on chromatin factors and the WAGO Argonaute pathway. Our findings support a model in which PRG-1 scans for foreign sequences and two other Argonaute pathways serve as epigenetic memories of "self" and "nonself" RNAs. These findings suggest how organisms can utilize RNAi-related mechanisms to detect foreign sequences not by any molecular signature, but by comparing the foreign sequence to a memory of previous gene expression. 相似文献
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Form, function, and regulation of ARGONAUTE proteins 总被引:2,自引:0,他引:2
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Liping Song Shijuan Gao Wei Jiang Shuai Chen Yanjun Liu Ling Zhou Wenlin Huang 《蛋白质与细胞》2011,2(4):273
RNA silencing is a conserved eukaryotic pathway involved in the suppression of gene expression via sequence-specific interactions that are mediated by 21–23 nt RNA molecules. During infection, RNAi can act as an innate immune system to defend against viruses. As a counter-defensive strategy, silencing suppressors are encoded by viruses to inhibit various stages of the silencing process. These suppressors are diverse in sequence and structure and act via different mechanisms. In this review, we discuss whether RNAi is a defensive strategy in mammalian host cells and whether silencing suppressors can be encoded by mammalian viruses. We also review the modes of action proposed for some silencing suppressors. 相似文献
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The diversity of RNA silencing pathways in plants 总被引:29,自引:0,他引:29
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Mechanisms of plant resistance to viruses 总被引:2,自引:0,他引:2
Plants have evolved in an environment rich with microorganisms that are eager to capitalize on the plants' biosynthetic and energy-producing capabilities. There are approximately 450 species of plant-pathogenic viruses, which cause a range of diseases. However, plants have not been passive in the face of these assaults, but have developed elaborate and effective defence mechanisms to prevent, or limit, damage owing to viral infection. Plant resistance genes confer resistance to various pathogens, including viruses. The defence response that is initiated after detection of a specific virus is stereotypical, and the cellular and physiological features associated with it have been well characterized. Recently, RNA silencing has gained prominence as an important cellular pathway for defence against foreign nucleic acids, including viruses. These pathways function in concert to result in effective protection against virus infection in plants. 相似文献
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Identification of an RNA silencing suppressor from a plant double-stranded RNA virus 总被引:9,自引:0,他引:9
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RNA silencing is a mechanism which higher plants and animals have evolved to defend against viral infection in addition to regulation of gene expression for growth and development. As a counterdefense, many plant and some animal viruses studied to date encode RNA silencing suppressors (RSS) that interfere with various steps of the silencing pathway. In this study, we report the first identification of an RSS from a plant double-stranded RNA (dsRNA) virus. Pns10, encoded by S10 of Rice dwarf phytoreovirus (RDV), exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c carrying GFP. The other gene segments of the RDV genome did not have such a function. Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA. Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N. benthamiana. Collectively, our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway. 相似文献
12.
Metzlaff M 《Biological chemistry》2002,383(10):1483-1489
In the 'RNA world' hypothesis it is postulated that RNA was the first genetic molecule. Recent discoveries in gene silencing research on plants, fungi and animals show that RNA indeed plays a key role not only in controlling invading nucleic acids, like viruses and transposable elements, but also in regulating the expression of transgenes and endogenous genes. Double-stranded RNAs were identified to be the triggering structures for the induction of a specific and highly efficient RNA silencing system, in which enzyme complexes, like Dicer and RISC, facilitate as 'molecular machines' the processing of dsRNA into characteristic small RNA species. RNA silencing can be transmitted rapidly from silenced to non-silenced cells by short and long distance signaling. There is evidence that at least one component of the signal is a specific, degradation-resistant RNA. 相似文献
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Genetic analysis of RNA-mediated transcriptional gene silencing 总被引:25,自引:0,他引:25
Matzke M Aufsatz W Kanno T Daxinger L Papp I Mette MF Matzke AJ 《Biochimica et biophysica acta》2004,1677(1-3):129-141
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RNA沉默在植物生物逆境反应中的作用 总被引:1,自引:0,他引:1
RNA沉默是真核生物共有的基因表达调节机制和防御机制。在植物RNA沉默中, 一些小RNAs, 如微小 RNAs和小干扰RNAs, 在植物防御病毒、细菌或食草动物的反应中具有重要作用。为了抑制宿主的RNA沉默系统, 植物病毒或细菌进化出了在RNA沉默不同阶段起作用的病毒沉默抑制子或细菌沉默抑制子, 来克服寄主的RNA沉默反应。文章就植物RNA沉默、病毒沉默抑制子、细菌沉默抑制子及其相关防御反应的一些新进展做一概述。 相似文献
15.
RNA interference is an exciting field of functional genomics that can silence viral genes. This property of interfering RNA can be used to combat viral diseases of plants as well as animals and humans. It is a short sequence of nucleic acid that can bind to the mRNA of the gene and interferes the process of its expression. It is diverse in occurrence as well as in applications. It occurs from nematodes to fungi and can cause gene silencing in plants, animals and human beings. Small interfering RNAs are used to silence plant viral genes and in production of therapeutic drugs against Hepatitis or Immuno-deficiency viruses in human. In this review, we will discuss the history, mechanism and applications of RNA interference in plant, animal and human research. 相似文献
16.
Fighting geminiviruses by RNAi and vice versa 总被引:3,自引:0,他引:3
Geminiviruses have recently emerged not only as the cause of devastating diseases of important crop plants but also as a tool
to study fundamental aspects of RNA interference (RNAi) and virus-induced gene silencing. RNA silencing is an evolutionary
conserved mechanism protecting cell from pathogenic RNA and DNA, which is increasingly viewed as an adaptive immune system
of plants against viruses. Here we summarize recent developments in the field of geminivirology presented by several leading
groups at the Meeting “Gemini2004” (a total of 85 participants from all over the world) with the main focus on the anti-viral
strategies that exploit RNAi and related silencing phenomena. 相似文献
17.
Identification of a novel RNA silencing suppressor,NSs protein of Tomato spotted wilt virus 总被引:14,自引:0,他引:14
Takeda A Sugiyama K Nagano H Mori M Kaido M Mise K Tsuda S Okuno T 《FEBS letters》2002,532(1-2):75-79
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Several years ago it was discovered that plant transformation with a transcribed sense transgene could shut down the expression of a homologous endogenous gene. Moreover, it was shown that the introduction into the cell of dsRNA (double-stranded RNA) containing nucleotide sequence complementary to an mRNA sequence causes selective degradation of the latter and thus silencing of a specific gene. This phenomenon, called RNA interference (RNAi) was demonstrated to be present in almost all eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggesting strong interconnections between RNAi and other metabolic pathways. RNAi is probably an ancient mechanism that evolved to protect eukaryotic cells against invasive forms of nucleic acids. 相似文献
20.
RNA沉默在机体防御病毒入侵和调控基因表达中发挥着重要的作用,目前已成为一种有效的工具应用于基因功能研究和疾病治疗等领域.RNA沉默现象普遍存在于真核细胞中,然而在宿主与病毒漫长的进化过程中,病毒已经演化出一系列逃逸或抑制RNA沉默作用的方法和途径,使RNA沉默效果显著降低,另一方面,哺乳动物体细胞自身也存在调节RNA沉默功能从而使生命活动的调节更加精细完善.为了使RNA沉默发挥它的潜在效应,人们设计出一系列的策略针对抑制RNA沉默效应以达到弱化抑制的目的.全面总结抑制RNA沉默机制及其应用,从而使人们充分认识到使用RNA沉默技术时应考虑到存在的不利因素. 相似文献