High-frequency surface waves at a plasma-metal interface: I. Linear model

A study is made of the dispersion properties of surface waves at a plasma-metal interface under thermodynamically nonequilibrium conditions such that a space charge sheath forms at the plasma boundary. In the simplest model, the sheath is described as a dielectric with a given permittivity. The wave parameters in a highly collisional plasma are discussed. The effect of interaction between waves propagating near the opposite plasma boundaries is considered, in particular, for space charge sheaths of different thicknesses. Conditions are determined under which the parameters of surface waves are substantially altered by the plasma-sheath geometric resonance.     

Thermal nonlinearity of potential surface waves at a plasma-metal interface

A study is made of the effect of the heating of plasma electrons in the field of a potential surface wave on the wave dispersion properties. The wave is assumed to propagate along the boundary between a metal and a finite-pressure plasma. Different mechanisms for electron energy losses are considered in the weak heating approximation. The spatial distribution of the plasma electron temperature under nonlocal heating conditions is derived on the basis of the electron energy balance equation. Expressions for the spatial damping rate and the nonlinear shift of the wavenumber are analyzed for different values of the plasma parameters. The results obtained are valid for both semiconductor and gaseous plasmas.     

Resonant excitation of counterpropagating surface waves at a Langmuir wave decay

A study is made of the resonant excitation of surface waves via the decay of a Langmuir wave propagating perpendicularly to a plasma-dielectric interface. The excitation dynamics in the initial stage of decay instability is studied, expressions for the growth rates are obtained, and the threshold amplitude of the Langmuir wave above which parametric instability occurs is found. Published in Russian in Fizika Plazmy, 2007, Vol. 33, No. 4, pp. 346–351. The article was translated by the author.     

Theory of transverse surface electromagnetic waves propagating along a plasma-metal boundary with a finite radius of curvature in a magnetic field

The spectra of electromagnetic waves propagating perpendicular to the axis of a plasma-filled metal waveguide in a magnetic field are studied with allowance for the effects exerted upon the wave frequency by the radial plasma density variation and by the emission of waves through a narrow axial slit in a waveguide wall. The case of wave propagation along the boundary between a plasma and a cylindrical metal waveguide wall with a periodically varying radius of curvature is also considered. The electromagnetic properties of the plasma are described by a dielectric tensor in the hydrodynamic approximation. The spatial distribution of the wave field is determined by the method of successive approximations. Results are presented from both analytical and numerical investigations. Analytical expressions for the corrections to the wave frequency due to the emission of the wave energy from the waveguide and due to the slight corrugation of the waveguide wall are obtained. The rates of wave damping due to the emission of the wave energy through a narrow axial slit and due to collisions between the plasma particles are found. The correction to the frequency that comes from the periodic variation of the radius of curvature of the plasma surface is calculated to within terms proportional to the square of the small parameter describing the azimuthal corrugation of the waveguide wall. The effect of the radial plasma density variation on the dispersion of the surface modes is examined both analytically and numerically.     

Finite element stress analysis of a push-out test. Part II: Free interface with nonlinear friction properties.

In this second part of a two-part paper, nonlinear frictional properties measured at the bone/porous-surfaced metal interface are used to perform the stress analysis of a push-out test assuming free interface. In this case, the friction at the interface is the only mechanism to resist the externally applied load. Similar to the part I, the model is axisymmetric and consists of two cylinders in contact with each other through the interface. Various relative material properties and boundary conditions are simulated in order to examine their effects on the interface stresses and overall push-out resistance. The role of the force-fit and the load direction (push-out versus pull-out) on the results is also investigated. The computed radial and shear stresses are found to markedly vary both with location along the interface and with the testing configuration. The ultimate push-out resistance is also found to significantly alter as the material arrangement and boundary conditions change. The predicted push-out load augments with an increase in the force-fit and diminishes to nil in the absence of a press-fit. For the cases studied here, there is a relative difference of as large as 13 percent between the push-out response and the pull-out response so far as the interface stresses and the maximum resistance are concerned. Therefore, any comparison between the results of push-out (or pull-out) tests performed with different design configurations appears to be invalid.     

Force measurements of Myosin II waves at the yolk surface during Drosophila dorsal closure

The mechanical properties and the forces involved during tissue morphogenesis have been the focus of much research in the last years. Absolute values of forces during tissue closure events have not yet been measured. This is also true for a common force-producing mechanism involving Myosin II waves that results in pulsed cell surface contractions. Our patented magnetic tweezer, CAARMA, integrated into a spinning disk confocal microscope, provides a powerful explorative tool for quantitatively measuring forces during tissue morphogenesis. Here, we used this tool to quantify the in vivo force production of Myosin II waves that we observed at the dorsal surface of the yolk cell in stage 13 Drosophila melanogaster embryos. In addition to providing for the first time to our knowledge quantitative values on an active Myosin-driven force, we elucidated the dynamics of the Myosin II waves by measuring their periodicity in both absence and presence of external perturbations, and we characterized the mechanical properties of the dorsal yolk cell surface.     

Excitation of surface waves at a plasma boundary by a short laser pulse

The excitation of surface waves by a laser pulse as it crosses a vacuum-plasma interface is considered. Surface waves are excited by a vortex electric current that is generated at the plasma boundary by the ponderomotive force of the pulse. The question is considered of how the duration and transverse dimensions of the pulse affect the spatiotemporal distribution and the spectral and energy parameters of the excited surface waves.     

Parametric excitation of surface waves at the boundary between a magnetized plasma and a metal

A study is made of the parametric excitation of potential surface waves propagating in a planar plasma-metal waveguide structure in a magnetic field perpendicular to the plasma-metal boundary. An external, spatially uniform, alternating electric field at the second harmonic of the excited wave is used as the source of parametric excitation. A set of equations is derived that describes the excitation of surface waves due to the onset of decay instability. Expressions for the growth rates in the linear stage of instability are obtained, and the threshold amplitudes of the external electric field above which the parametric instability can occur are found. Analytic expressions for the saturation amplitudes are derived with allowance for the self-interaction of each of the excited waves and the interaction between them. The effect of the plasma parameters and the strength of the external magnetic field on the saturation amplitude, growth rates, and the threshold amplitudes of the pump electric field are analyzed.     

Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface.

The role of an 18-residue ion-pair network, that is present in the glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus, in conferring stability to other, less stable homologous enzymes, has been studied by introducing four new charged amino acid residues into the subunit interface of glutamate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima. These two GDHs are 55 % identical in amino acid sequence, differ greatly in thermo-activity and stability and derive from microbes with different phylogenetic positions. Amino acid substitutions were introduced as single mutations as well as in several combinations. Elucidation of the crystal structure of the quadruple mutant S128R/T158E/N117R/S160E T. maritima glutamate dehydrogenase showed that all anticipated ion-pairs are formed and that a 16-residue ion-pair network is present. Enlargement of existing networks by single amino acid substitutions unexpectedly resulted in a decrease in resistance towards thermal inactivation and thermal denaturation. However, combination of destabilizing single mutations in most cases restored stability, indicating the need for balanced charges at subunit interfaces and high cooperativity between the different members of the network. Combination of the three destabilizing mutations in triple mutant S128R/T158E/N117R resulted in an enzyme with a 30 minutes longer half-life of inactivation at 85 degrees C, a 3 degrees C higher temperature optimum for catalysis, and a 0.5 degrees C higher apparent melting temperature than that of wild-type glutamate dehydrogenase. These findings confirm the hypothesis that large ion-pair networks do indeed stabilize enzymes from hyperthermophilic organisms.     

Sensory structures at the surface of fish skin. II. Lateralis System

Scanning electron microscopy shows the form of the cupulae of free neuromasts in two species of teleost fish, and gives information about the organization of the free neuromasts in teleosts and lampreys. In lampreys some neuromasts were found to lack the surrounding moat and the flanking hillocks characteristic of the lateral line organs previously described in these fish. In all cases, the sensory cells had the kinocilium aligned with respect to the stereocilia on the longer axis of the neuromast surface, thus enabling the direction of effective stimulation of the free neuromasts to be deduced from their morphological arrangement.     

Adhesion of Leptospira at a solid-liquid interface: a model

Two strains of the saprophytic Leptospira biflexa serovar patoc display reversible and irreversible adhesion at a solid-liquid interface. Both forms of adhesion are enhanced in the presence of 20 M carbonyl cyanide metachlorophenyl hydrazone (CCCP), an uncoupler which inhibits motility of the bacteria. Microscopic observations also indicated that motility may have a role in adhesion as only actively motile organisms were seen to detach from the substratum. A dynamic model is proposed for adhesion of these organisms at a solid-liquid interface. It is suggested that the level of reversible adhesion is determined by the comparative rates of attachment (ON phase) and detachment (OFF phase). As reversible adhesion is mediated by weak forces of attraction, bacterial motility or gentle washing could promote the OFF phase. When motility is inhibited, the OFF phase is reduced and the ON phase continues (as motility is not required for the ON phase) causing the level of reversible adhesion to increase. Since reversible adhesion is a prerequisite for irreversible adhesion, then increased reversible adhesion leads directly to increased irreversible adhesion. Reversible adhesion appears to be mediated by the weak attractive forces of the secondary minimum whereas the mechanism facilitating irreversible adhesion of leptospires is not known.Abbreviation CCCP carbonyl cyanide meta-chlorophenyl hydrazone     

VIR.II: a new interface with the antibody sequences in the Kabat database.

The Kabat database is the source of information par excellence on antibody sequences. In 1995, we developed an interface with the Kabat database, called VIR. VIR has been very useful in conducting studies aiming to find structure-function relationships in antibodies. Here we report a new version adapted to the World Wide Web, called VIR.II. VIR.II allows searches by type of chain (V(H) or V(L)), by species, and by specificity. The species are selected using a pulldown menu, whereas the specificities can be selected from a list containing the unique specificities reported in the Kabat database. These facilities avoid mistakes and redundancies in the searches. Another feature, and probably the most important one, is that VIR.II introduces a classification of specificities in terms of the chemical and biochemical nature of the antigen, like anti-protein, anti-peptide, anti-hapten, etc. This classification has been useful in discovering patterns in the antigen-binding site of antibodies that correlate with the type of antigen the antibody interacts with. To illustrate this, while showing the capabilities of VIR.II, we analyze all the murine anti-peptide and anti-protein antibody sequences compiled as of July, 2000 in the Kabat database.     

Excitation of high-frequency azimuthal surface waves by an annular electron beam in a waveguide with a noncircular interface of the plasma column

An initial stage of the interaction of an electron beam ring rotating along Larmor orbits in a gap between the plasma column and a circular metal chamber of a cylindrical waveguide with extraordinarily polarized electromagnetic waves of the surface type is studied. These waves propagate along the azimuthal angle across an axial magnetic field in the range above the upper hybrid frequency. Using numerical analysis of the dispersion relation, it is shown that by the aid of an appropriate choice of the shape of the plasmavacuum interface one can achieve a significant increasing of growth rates of the resonant beam instability of these waves.     

Complex carbohydrate-lectin interaction at the interface: a model for cellular adhesion. II. Reactivity of both the oligosaccharide chain and sugar-binding domain of a glycoprotein lectin.

We describe studies of a new model cell adhesion system involving liposomes bearing lectins and the glycosphingolipid, asialomonosialoganglioside (asialoGM1). The model provides a simple analysis of experimental data to elucidate the mechanism of heterophilic cell-cell adhesion mediated by multiple protein-carbohydrate interactions. Phospholipid vesicles bearing the fatty acid conjugate of a glycoprotein lectin from Ricinus communis (RCAI vesicle) are shown to react with vesicles bearing the fatty acid conjugate of Concanavalin A (Con A) and asialoGM1 (Con A vesicle). The kinetics of aggregation and monosaccharide-induced disaggregation of the two types of vesicles were followed by monitoring the time-dependent change in turbidity. Depending on the surface density of the asialoGM1, 40-60% of the resulting precipitin complex was dissociable only in the presence of both RCAI-specific galactose and Con A-specific alpha-methyl-D-mannoside. Results indicate simultaneous participation of both the saccharide-binding domain and carbohydrate sequence of RCAI, a model cell adhesion molecule, to stabilize the encounter complex by two types of interactions. These findings support the possibility of stable cell-cell adhesion in vivo occurring via interactions between cell adhesion molecules on apposing cell-surface membranes.     

Role of surface chemistry in protein remodeling at the cell-material interface

Background

The cell-material interaction is a complex bi-directional and dynamic process that mimics to a certain extent the natural interactions of cells with the extracellular matrix. Cells tend to adhere and rearrange adsorbed extracellular matrix (ECM) proteins on the material surface in a fibril-like pattern. Afterwards, the ECM undergoes proteolytic degradation, which is a mechanism for the removal of the excess ECM usually approximated with remodeling. ECM remodeling is a dynamic process that consists of two opposite events: assembly and degradation.

Methodology/Principal Findings

This work investigates matrix protein dynamics on mixed self-assembled monolayers (SAMs) of –OH and –CH₃ terminated alkanethiols. SAMs assembled on gold are highly ordered organic surfaces able to provide different chemical functionalities and well-controlled surface properties. Fibronectin (FN) was adsorbed on the different surfaces and quantified in terms of the adsorbed surface density, distribution and conformation. Initial cell adhesion and signaling on FN-coated SAMs were characterized via the formation of focal adhesions, integrin expression and phosphorylation of FAKs. Afterwards, the reorganization and secretion of FN was assessed. Finally, matrix degradation was followed via the expression of matrix metalloproteinases MMP2 and MMP9 and correlated with Runx2 levels. We show that matrix degradation at the cell material interface depends on surface chemistry in MMP-dependent way.

Conclusions/Significance

This work provides a broad overview of matrix remodeling at the cell-material interface, establishing correlations between surface chemistry, FN adsorption, cell adhesion and signaling, matrix reorganization and degradation. The reported findings improve our understanding of the role of surface chemistry as a key parameter in the design of new biomaterials. It demonstrates the ability of surface chemistry to direct proteolytic routes at the cell-material interface, which gains a distinct bioengineering interest as a new tool to trigger matrix degradation in different biomedical applications.     

Phospholipid surface bilayers at the air-water interface. III. Relation between surface bilayer formation and lipid bilayer assembly in cell membranes.

Lipid bilayer assembly in cell membranes has been simulated with total lipid extracts from human red blood cells and from mesophilic and thermophilic bacteria grown at several temperatures. Aqueous dispersions of these natural lipid mixtures form surface bilayers, a single bimolecular lipid state, but only at the growth temperature of the source organism. Thus, a single isolated bilayer state forms spontaneously in vitro from lipids that are available in vivo at the growth temperature of the cell. Surface bilayers form at a specific temperature that is a function of hydrocarbon chain length and degree of fatty acid unsaturation of the phospholipids; this property is proposed as an essential element in the control of membrane lipid composition.     

Deep-Sea Macrobenthic Communities at Contrasting Sites off Portugal,Preliminary Results: II Spatial Dispersion

Small-scale, biogenic processes are thought to be important in creating a mosaic of microhabitats that allow co-existence of the large numbers of rare species found in deep-sea sediments. However, the large-scale effects of hydrodynamic disturbance seem likely to be important in high-energy settings on the deep-sea bed. This is investigated by examining spatial dispersion patterns in the macrobenthic populations at two deep-sea sites off Portugal. The widely differing conditions of sediment disturbance at the two sites are related to differences in macrobenthic species diversity and dominance. Closely grouped, transponder-mapped, replicate box core samples, each divided into 25 “vegematic” subcores permitted analysis of dispersion patterns at two spatial scales at each site. One site was located on the Tagus Abyssal Plain (TAP), and the other in the nearby Setubal Canyon (SC) where ripple bedforms indicate vigorous bed flow, probably with a tidal periodicity. Greater aggregation at the scale of tens of metres is indicated from the observed numbers of SC species per box core being wide underestimates of the value predicted by rarefaction of the pooled total compared to those from TAP. Variance/mean ratios for individual species of bivalves and tanaids indicated more aggregation in spatial distribution between box cores from SC than from TAP. Dispersion chi-square analysis also indicated more non-randomness at SC between box cores than for the TAP cores. However, low faunal abundances in the TAP samples render the results of analyses for this site somewhat inconclusive and therefore the trend indicating less aggregation at TAP compared to SC must be regarded as weak for the groups examined. Analyses of the distribution of tanaids, and bivalves at a 100 × 100 mm subcore scale showed no convincing significant departure from random expectation, but low abundances in these groups reduces the sensitivity of the tests applied. Between-core aggregation detected at SC may reflect habitat variation resulting from the varying depth within the area of sampling, while within-core patchiness could be influenced by the ripple bed forms on the sediment.     

von Willebrand factor. A protein which binds at the cell surface interface between platelets

von Willebrand factor (VWF) functions in platelet aggregation, a form of cellular interaction. In vitro analysis of platelet aggregation, as measured by the platelet aggregometer, requires addition of a promoter such as the glycopeptide antibiotic ristocetin. Native multimeric VWF (Mr = 1-20 X 10(6)) can be reduced with sulfhydryl reagents to a monomeric state (Mr = 2 X 10(5)). In this study, the binding of bovine VWF and ristocetin to bovine platelets was investigated using fluorescence anisotropy of derivatized monomer protein and ristocetin and also by radioisotope methods using 125I-labeled monomer and native protein. Ristocetin bound to bovine platelets but not to VWF. VWF binding to formaldehyde-fixed platelets was dependent on the presence of a promoter such as ristocetin. The monomer and multimer VWF bound equally well in the presence of low ristocetin concentrations. Under these conditions, plots of VWF binding versus platelet concentration were sigmoidal, indicating positive cooperativity with respect to platelets. At higher (100 micrograms/ml) ristocetin concentrations, the binding curve was no longer sigmoidal. Ristocetin promoted the formation of small platelet aggregates, an effect that was amplified by the presence of VWF. In fact, all conditions which resulted in monomer or multimer VWF binding to platelets also caused formation of platelet aggregates observed by light microscopy. These combined results were consistent with VWF binding only to the interface between proximal platelets. High affinity binding could be provided by the presence of two cell surfaces and the resulting multiple binding interactions. Polycations, such as poly(L-lysine) and Polybrene, also promoted the formation of platelet aggregates and facilitated the binding of VWF to platelets. Physiological platelet activators such as thrombin, ADP, and collagen also facilitated VWF binding to native platelets and caused platelet aggregation. It appears possible that any process which causes the surface membranes of platelets to become spatially close will allow expression of VWF activity.     

Complex formation between chlorophyll a and cytochrome c: surface properties at the air-water interface. Absorbance, fluorescence and fluorescence-lifetime in Langmuir-Blodgett films.

The binding of cytochrome c to an insoluble monolayer of chlorophyll a was studied. Surface pressure (II), surface potential (delta V) and [14C]cytochrome c surface-concentration (gamma) isotherms were measured versus molecular area (sigma) in mixed films. Compared to the successive-addition method, this procedure allows the formation of homogeneous mixed films. The cytochrome c is incorporated into a chlorophyll a monolayer, compressed at a surface pressure of 20 mN.m-1. On expansion, the quantity of protein incorporated into the monolayer gradually increases. Subsequent compression-expansion cycles result in similar isotherms, distinct from that measured during the first expansion. All surface properties measured, but more specifically the surface radioactivity of [14C]cytochrome c, indicate the irreversibility of protein incorporation into the chlorophyll a monolayer. In fact, surface properties of the binary film are completely different from the properties of either of the pure components. As a result, calculated values of surface potentials for mixed films using the additivity law deviate from experimentally measured potentials. The absorption and fluorescence spectra of mixed films transferred onto a solid substrate by the Langmuir-Blodgett technique, indicate a dilution effect of chlorophyll a by cytochrome c. However, the dilution effect cannot be detected by the fluorescence lifetimes of pure chlorophyll a and mixed chlorophyll a-cytochrome c films, both shorter than 0.2 ns. This provides support for the existence of an energy-transfer mechanism between chlorophyll a monomer and chlorophyll a aggregates which could serve as an energy trap. The role of the protein could be related to that of the matrix.