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Identification of Phenanthrene Derivatives in Aerides rosea (Orchidaceae) Using the Combined Systems HPLC–ESI–HRMS/MS and HPLC–DAD–MS–SPE–UV–NMR 下载免费PDF全文
Veronika Cakova Aurélie Urbain Cyril Antheaume Nicole Rimlinger Patrick Wehrung Frédéric Bonté Annelise Lobstein 《Phytochemical analysis : PCA》2015,26(1):34-39
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Bin Li Xu Zhang Juan Wang Le Zhang Bowen Gao Shepo Shi Xiaohui Wang Jun Li Pengfei Tu 《Phytochemical analysis : PCA》2014,25(3):229-240
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Yichun Xu Guanglin Xu Li Liu Desheng Xu Jianwen Liu 《Journal of cellular biochemistry》2010,111(2):370-379
Rosmarinic acid is a major phenylpropanoid isolated from Prunella vulgaris L., which is a composition of herbal tea for centuries in China. However, the anti‐invasion activity on Ls174‐T human colon carcinoma cells has not been studied. In this study, we investigated the anti‐metastasis functions according to wound healing assay, adhesion assay, and Transwell assay and found that rosmarinic acid could inhibit migration, adhesion, and invasion dose‐dependently. Rosmarinic acid also could decrease the level of reactive oxygen species by enhancing the level of reduced glutathione hormone. In addition, rosmarinic acid repressed the activity and expression of matrix metalloproteinase‐2,9. According to Western blot and quantitative real‐time PCR assay, rosmarinic acid may inhibit metastasis from colorectal carcinoma mainly via the pathway of extracellular signal‐regulated kinase. In animal experiment, intraperitoneal administration of 2 mg of rosmarinic acid reduced weight of tumors and the number of lung nodules significantly compared with those of control group. Therefore, these results demonstrated that rosmarinic acid can effectively inhibit tumor metastasis in vitro and in vivo. J. Cell. Biochem. 111: 370–379, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
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HPLC‐UV and LC–MS Analyses of Acylquinic Acids in Geigeria alata (DC) Oliv. & Hiern. and their Contribution to Antioxidant and Antimicrobial Capacity 下载免费PDF全文
Dimitrina Zheleva‐Dimitrova Reneta Gevrenova Maya M. Zaharieva Hristo Najdenski Silviya Ruseva Valentin Lozanov Vessela Balabanova Sakina Yagi Georgi Momekov Vanio Mitev 《Phytochemical analysis : PCA》2017,28(3):176-184
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Jun Ding Li‐Jing Mao Shao‐Ting Wang Bi‐Feng Yuan Yu‐Qi Feng 《Phytochemical analysis : PCA》2013,24(4):386-394
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Identification of N‐octylnortadalafil and its Stereoisomers in Dietary Supplements with Chiral Liquid Chromatography–Circular Dichroism 下载免费PDF全文
A direct chiral liquid chromatography–circular dichroism (LC‐CD) method was developed for the simple and rapid identification of N‐octylnortadalafil [(6R, 12aR)‐6‐(1,3‐benzodioxol‐5‐yl)‐2‐octyl‐2,3,6,7,12,12a‐hexahydropyrazino[1’,2’:1,6]pyrido[3,4‐b]indole‐1,4‐dione; RR‐OTDF] and its stereoisomers in dietary supplements. Samples were extracted with methanol. Compounds were then separated by chiral LC‐CD using Chiralcel OD‐RH (4.6 × 1 50 mm, 5 µm) with 5 mM ammonium formate (pH 3)/0.1% formic acid in acetonitrile (95:5, v/v) mixture solution (mobile phase A) and 0.1% formic acid in acetonitrile (mobile phase B). The isocratic elution used was mobile phase A / mobile phase B (3:7, v/v) at a flow rate of 1 ml/min. The column temperature was held at 30°C. RR‐OTDF and its stereoisomers were separated within 20 min with the resolution factors being over 2.0. Using this method, RR‐OTDF and (6R, 12aS)‐6‐(1,3‐benzodioxol‐5‐yl)‐2‐octyl‐2,3,6,7,12,12a‐hexahydropyrazino[1’,2’:1,6]pyrido[3,4‐b]indole‐1,4‐dione were detected in a dietary supplement. Chirality 28:204–208, 2016. © 2016 Wiley Periodicals, Inc. 相似文献