两种漱口液对口腔挥发性硫化物及舌背细菌的影响

目的评价常见的两种市售漱口液对口腔挥发性硫化物水平及舌背菌群的影响。方法采用为期1月的单中心、随机、对照、双盲的临床研究方法。研究对象共60人。分为李斯德林漱口液组(A组),高露洁贝齿漱口液组(B组),阴性对照组(C组)。分别在基线、半个月、1个月时检测各组研究对象的口腔挥发性硫化物水平及舌背厌氧菌的数量变化,并进行统计学分析处理。结果对于口腔挥发性硫化物水平,在半个月及1个月时,A组、B组均有明显的下降,均优于C组,差异具有统计学意义(P<0.05);且A组与B组之间的差异也具有统计学意义(P<0.05)。对于舌背厌氧菌的数量变化,在半个月及1个月时,A组、B组厌氧菌数量均有所下降,均优于C组,差异具有统计学意义(P<0.05);但A组与B组之间的差异没有统计学意义(P>0.05)。结论李斯德林漱口液及高露洁贝齿漱口液均能有效降低口腔挥发性硫化物水平及舌背厌氧菌的数量,且李斯德林漱口液降低硫化物的效果更优。     

固定矫治器对牙周指数及龈下菌群影响的实验研究

选择15例口腔卫生良好、牙周组织健康的正畸患者的90个牙位点,在黏接固定矫治器附件前进行菌斑指数、牙龈指数和探诊深度等临床牙周指数及龈下细菌的检测;根据患者上下颌牙齿错位畸形严重程度进行单颌牙齿黏接固定矫治器附件作为实验组,以暂不黏接固定矫治器附件的对颌牙齿作为对照组,在患者4周、8周复诊时进行重复检测。结果显示,戴用固定矫治器后牙齿的临床牙周指数与对照组及戴用矫治器前相比较,没有显著性差异(p>0.05);龈下细菌构成及比例亦未发生明显变化(p>0.05)。研究表明,戴用固定矫治器的患者如能认真保持口腔卫生,对牙周组织健康不会产生显著不良影响。     

植体后牙周病应用益生菌辅以基础治疗对患者牙周指数及炎性因子影响

目的 观察在基础治疗中加用益生菌治疗对改善植体后牙周炎患者牙周指数、炎性因子及植体的影响。方法 分析本院2021年4月至2022年6月收治的76例植体后牙周病患者的临床资料,根据不同的治疗方法分为对照组（n=37,牙周基础治疗）和研究组[n=39,益生菌（口服,1次/片,2次/d）辅以牙周基础治疗]。比较2组研究对象临床疗效、牙周指数、炎性因子、舌苔与口气指标及不良反应,同时记录治疗过程中种植体存留率情况。结果 研究组患者治疗后总有效率为97.44%,对照组为81.08%,对照组显著低于研究组（P<0.05）。治疗前,2组患者牙周指数、炎性因子以及舌苔与口气指标水平比较差异均无统计学意义（均P>0.05）;治疗后,2组患者炎性因子（IL-6、IL-1β、MMP-9、TNF-α）、牙周指数（PD、PLI、BI）和舌苔与口气指标（VSCs、OS）水平均下降,且研究组上述指标均低于对照组（均P<0.05）。治疗期间,研究组患者不良反应发生率为10.26%,对照组为8.11%,组间比较差异无统计学意义（P>0.05）。2组患者治疗过程中,通过影像学检查结果提示种植牙体未...     

地塞米松漱口液应用于鼻咽癌放疗的临床观察

目的 观察应用含地塞米松漱口液在减轻鼻咽癌放疗反应中的疗效.方法 将60例鼻咽癌患者随机分为2组,治疗组31例在放疗期间予含有地塞米松的漱口液含漱,对照组29例子不含地塞米松漱口液含漱.结果 治疗组轻度反应18例（58%）,中度反应10例（32）,重度反应3例（10%）;对照组无轻度反应,中度反应12例（41%）.重度反应17例（59%）.结论 治疗组重度反应率占10%,对照组重度反应率占59%,治疗组重度反应明显低于对照组.患者全部能按计划完成全程放疗.     

RPDS基牙牙周指数及牙菌斑细菌相对分布的检测分析

目的　观察基牙牙周指数及牙菌斑细菌相对分布的动态变化,以深入了解ＲＰＤｓ 对口腔微生态系影响的机理及特点。方法　在预先规定的７ 个观察期,按照文献检测基牙菌斑指数（ＰＬＩ） 、龈炎指数（ＧＩ） 及牙龈出血指数（ＧＢＩ） ;采用刚果红负性染色涂片检查法,检测基牙龈下菌斑细菌相对分布变化。结果　①患者戴用ＲＰＤｓ 后短期内基牙ＰＬＩ、ＧＩ、ＧＢＩ均明显升高（Ｐ＜ ０．０１） ,且在前６ 个月内逐渐升高,６ 个月之后则有所降低并趋于平稳;②戴用ＲＰＤｓ后牙菌斑细菌中球菌比例减小,而梭杆菌和螺旋体的比例升高。结论：戴用ＲＰＤｓ 会引起基牙ＰＬＩ、ＧＩ、ＧＢＩ升高及牙菌斑细菌中致病细菌如螺旋体、梭状杆菌比例增加。这对认识ＲＰＤｓ 对口腔微生态系影响的机制及临床防治ＲＰＤｓ 所致口腔病变具有有效的理论指导价值。     

脉冲式冲牙器预防正畸患者牙龈炎的纵向对比研究

目的:正畸患者口腔内环境往往会受到多种不良影响,造成大量微生物种群定植于牙龈周围,并形成牙菌斑改变牙周组织的形态和结构,对正畸效果及疗程产生较大不利因素,如何采用合理干预措施降低口腔正畸患者牙菌斑和牙龈炎的发生率,成为正畸是否成功的关键因素之一。本研究旨在探讨脉冲式冲牙器对正畸患者牙菌斑及牙龈炎症的影响。方法:以82例青少年正畸患者及健康志愿者为研究对象,将正畸患者随机分为研究组(41例)和对照组(41例);于正畸后30天后对患者进行口腔卫生宣教及日常刷牙方法系统培训,研究组正畸患者采用脉冲式冲牙器;于干预前后评价牙龈指数(gingival index,GI)和Quigley-Hein菌斑指数(plaque index,PI)。结果:在干预前,志愿者与正畸30天患者均存在不同程度口腔卫生问题,正畸患者GI及PI等指数明显高于健康人群,差异具有统计学意义(P0.05);经过一定周期的干预,两组正畸患者的GI均有不同程度的改善,但以研究组患者效果更为明显,差异具有统计学意义(P0.05)。结论:脉冲式冲牙器能够产生超细高压脉冲水柱,可以在正畸治疗期间有效提高菌斑清除率,减缓牙龈炎的进程,不同程度改善正畸患者口腔卫生,有助于提高正畸治疗效果。     

口泰含漱液对控制菌斑和牙龈炎的疗效分析

本研究采用双盲随机对照法观察口泰含液对牙龈炎及控制菌斑的疗效。结果显示：实验组的牙龈指数,龈沟出血指数及菌斑指数以在含漱５天后均有明显的改善,且与对照组的比较也有明显的差异。     

乳杆菌代谢产物对于固定矫治患者牙周指数和龈下菌群的敏感性研究

目的对已佩带固定矫治器6个月的患者采用乳杆菌DM9811代谢产物提取液制成的含漱液含漱治疗,探讨治疗前后牙周指数和牙周可疑病原菌的动态变化过程。方法采取自身前后对照和组间对照。记录治疗前后牙周指数的变化情况。并采用细菌培养鉴定法检测含漱前后龈下菌群的变化情况,测定牙周可疑病原菌的检出量和检出率。结果（1）试验组的牙龈指数（GI）、菌斑指数（PI）、龈沟出血指数（SBI）、探诊深度（PD）在治疗后均有下降,且试验组低于对照组。（2）试验组G^-产黑色素厌氧杆菌（BPAR）、梭杆菌、拟杆菌的检出量以及BPAR的检出率治疗后均有下降,试验组低于对照组;血链球菌的检出量有所上升,试验组高于对照组。结论乳杆菌DM9811代谢产物对固定矫治患者带环牙牙龈炎的各项临床指标均有改善作用,且颊侧优于舌侧;对龈下菌斑中梭杆菌、拟杆菌、BPAR等均有抑制作用,对有益菌中的血链球菌有扶植作用。     

妊娠中期牙龈炎女性唾液菌群的多样性研究

目的研究妊娠中期牙龈炎女性患者唾液菌群结构的多样性,为该类患者的治疗提供参考。方法 选取35例妊娠中期牙龈炎患者为研究组,15例妊娠中期健康女性为对照组。2组对象均取唾液样本,提取细菌DNA,进行PCR扩增与京都基因和基因组百科全书（KEGG）分析,采用Quantity One软件对指纹图谱进行数据分析。结果 研究组患者唾液菌群可操作分类单元（OTUs）数量多于对照组。研究组与对照组对象唾液菌群Alpha多样性、Beta多样性,物种相似性（门、纲、目、科、属、种水平）,KEGG基因通路距离及相对丰度比较差异均无统计学意义（均P>0.05）。研究组患者唾液菌群扩增序列变异数（ASV）分类单元水平（界、门、纲、目、科、属、种水平）和Proteobacteria、Campliobacterota、Pasteurellales、Pasteurellaceae、 Porphyromonadaceae、 Fusobacteriaceae、 Haemophius、 Porphyromonas、 Fusobacterium, Prevotella melaninogenica相对丰度均高于对照组...     

不同钙浓度对葛仙米三种酶活性的影响

本文对培养20天后葛仙米不同氯化钙浓度下的三种酶活性进行对比发现,超氧化物歧化酶(SOD)活性随着钙离子浓度的升高而降低,多酚氧化酶(PPO)活性随着钙离子浓度的升高而降低。苯丙氨酸转氨酶(PAL)在较低钙浓度下表现出较高的活性,而在较高的钙浓度下活性较低。     

Views on antioxidant mouthwashes as adjunct in periodontal therapy

Clinical decision is often difficult with chlorhexidine mouthwash. The use of antioxidant mouthwashes for the treatment of periodontal disease is in practise. Therefore, it is of interest to collect gleaned information on Antioxidant mouthwashes as periodontal therapy from known literature. Improvement in treatment using antioxidant mouthwashes is reported in several studies. The mouthwash with antioxidants has similar anti-gingivitis, antiplaque and antimicrobial effects as that of chlorhexidine mouthwash.     

口腔卫生预防措施对固定正畸患者牙周菌群和牙周临床指数的影响

目的 采用牙周洁治和强化的口腔健康教育方法,通过检测牙周菌群和牙周临床指数的动态变化,探讨口腔卫生预防措施对固定正畸患者牙周菌群的影响。方法 选择正畸初诊患者20例,平均分为试验组（T组）和对照组（C组）。于正畸治疗开始前检查右上颌第一磨牙16和右下颌中切牙41牙周状况,并采集其近中颊轴嵴处龈缘菌斑做细菌分离培养。T组于治疗前进行全口洁治,每月复诊加力时均给予口腔卫生检查,强调口腔卫生的重要性;C组仅在初诊时进行口腔卫生指导,其余不作处理。2组患者分别于矫治器安装后1、3和6个月进行临床及细菌学检查。结果 随观察时间的延长,T组颊侧菌斑指数和牙龈指数在第1、3、6个月时较基线降低;C组颊侧菌斑指数第6个月时较基线降低,舌侧探诊深度则升高（P＜0.05）。细菌检出率和检出量的变化在T组可见韦荣球菌属降低而弯曲杆菌属和Gn产黑色素厌氧杆菌（BPAR）升高,C组消化链球菌属和BPAR升高（P＜0.05）;BPAR在第3个月、消化链球菌属在第6个月时T组检出率低于C组（P＜0.05）,而细菌检出量和牙周临床指数在2组间没有观测到处理因素的作用（P＞0.05）。结论 正畸前即存在牙龈炎的患者,建议进行预防性牙周洁治;牙周洁治必须和口腔卫生教育、正确的日常菌斑控制措施结合进行。     

Impact of three different types of exercise on components of the inflammatory response

It was hypothesized that muscle injury would be greater with eccentric than with all-out or prolonged exercise, and that immune changes might provide an indication that supplements the information provided by traditional markers such as creatine kinase (CK) or delayed-onset muscle soreness. Eight healthy males [mean (SE): age = 24.9 (2.3) years, maximum oxygen consumption (VO2(max)) = 43.0 (3.1) ml x kg(-1) x min(-1)] were each assigned to four experimental conditions, one at a time, using a randomized-block design: 5 min of cycle ergometer exercise at 90% VO2(max) (AO), a standard circuit-training routine (CT), 2 h cycle ergometer exercise at 60% VO2(max) (Long), or remained seated for 5 h. Blood samples were analyzed for CK, natural killer (NK) cell counts (CD3(-)/CD16(+)56(+)), cytolytic activity and plasma levels of the cytokines interleukin (IL)-6, IL-10, and tissue necrosis factor alpha (TNF-alpha). CK levels were only elevated significantly 72 h following CT. NK cell counts increased significantly during all three types of exercise, but returned to pre-exercise baseline values within 3 h of recovery. Cytolytic activity per NK cell was not significantly modified by any type of exercise. Prolonged exercise induced significant increases in plasma IL-6 and TNF-alpha. We conclude that the lack of correlation between traditional markers of muscle injury (plasma CK concentrations and muscle soreness rankings) and immune markers of the inflammatory response suggests that, for the types and intensities of exercise examined in this study, the exercise-induced inflammatory response is modified by humoral and cardiovascular correlates of exercise.     

Occurrence of herpes simplex virus 1 and three periodontal bacteria in patients with chronic periodontitis and necrotic pulp

Viral and bacterial associations appear to be implicated in the development of periodontal infections. Little information is available describing the periodontopathic agents in root canals with necrotic pulp. In this study, the occurrence and the combinations among herpes simplex virus type 1 (HSV-1) and Dialister pneumosintes, Tannerella forsythia, and Treponema denticola in patients with chronic periodontitis and necrotic pulp were evaluated. Clinical samples from healthy subjects and patients with periodontal or pulp infections were analyzed using a nested polymerase chain reaction PCR to detect HSV and PCR to detect the 3 periodontal bacteria. The presence of Tannerella forsythia and Treponema denticola was observed in healthy, periodontitis, and necrotic pulp patients. HSV was observed in periodontitis and necrotic pulp patients, and no healthy subject harbored D. pneumosintes or HSV. The occurrence of Tannerella forsythia was not statistically significant in patients with necrotic pulp (P = 0.704). Periodontal bacteria were observed varying from 10.3% to 20.7% in periodontitis and necrotic pulp patients. The presence of Treponema denticola - HSV association was predominant in patients showing necrotic pulp (24.1%); however, HSV alone was observed in one patient with periodontitis and in another patient with necrotic pulp. The presence of double association among bacteria or bacteria - HSV could indicate a role in both periodontitis and necrotic pulp, and Tannerella forsythia - Treponema denticola - HSV and Tannerella forsythia - D. pneumosintes - Treponema denticola - HSV associations might be important in periodontitis.     

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

The aim was to investigate the detection rates of periodontal bacteria (Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans) and herpesviruses (herpes simplex virus-1 [HSV-1], cytomegalovirus [CMV], and Epstein-Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty-nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end-point polymerase chain reaction analyses. The results revealed significantly higher detection rates of P. gingivalis, T. forsythia, and P. intermedia in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of A. actinomycetemcomitans was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and CMV in patients with severe forms of periodontitis than in healthy participants and those with moderate periodontitis.     

Impact of oxygenation on the performance of three non-Saccharomyces yeasts in co-fermentation with Saccharomyces cerevisiae

Applied Microbiology and Biotechnology - The sequential or co-inoculation of grape must with non-Saccharomyces yeast species and Saccharomyces cerevisiae wine yeast strains has recently become a...     

FEA analysis of Normofunctional forces on periodontal elements in different angulations

In the literature, the periodontal tissue reaction to dissimilar occlusal stress has been described, including clinical and histologic changes caused by stresses in periodontal structures. With respect to occlusal forces, periodontal assembly demonstrates varying adaptive capacity from individual to individual and period to period within the same individual. Unfortunately, these occlusal stresses are yet to be quantified. As a result, determining the effect of normal occlusal force on periodontal elements in various angulations is of interest. Based on CBCT images, one FEA of the maxillary First molar was created, consisting of tooth pulp, periodontal ligament (PDL), and alveolar bone; the effect of normal occlusal force on the pdl in alternate angulations was assessed. Occlusion will occur at three contact areas representing the centric occlusion contact points, each of which will share a 150 N force. The analysis was performed for four force inclinations (0, 22.5°, 45°, and 90°). Maximum stresses are observed in cases of 90-degree loading. These stresses, however, are insignificant and will not cause the periodontal components to rupture. These tensile stresses, which are concentrated in the apical and cervical regions, may obstruct blood flow, resulting in tooth decay or, in some cases, periodontal breakdown in PDL. There have been attempts to express numerical data of stress to be provided for normal and hyper function loads to simulate occlusal situations at various angulations that are known to be accountable for healthy and diseased periodontium.     

不同质子泵抑制剂对冠脉支架植入术后氯吡格雷联合阿司匹林抗血小板作用的研究

目的:通过比较奥美拉唑和泮托拉唑对冠状动脉支架术(PCI)后患者血小板功能指标和主要不良心血管事件与出血并发症发生情况,探讨不同质子泵抑制剂对PCI后氯吡格雷联合阿司匹林抗血小板作用的影响。方法:60例实施PCI后常规联合抗血小板治疗(氯吡格雷75mg/d+阿司匹林100mg/d)患者随机分为奥美拉唑组(40mg/d,20例),泮托拉唑组(40mg/d,20例)和对照组(20例),连续用药30d。分别在服药前1d及服药15d,30d用血栓弹力图检测ADP途径诱导的血小板抑制率值和比浊法检测ADP途径诱导的血小板最大聚集率(MPAR)。并观察30d各组主要不良心血管事件和出血并发症的发生情况。结果:①奥美拉唑组和泮托拉唑组与对照组相比,服药前1d及服药15d,30d用血栓弹力图检测的血小板抑制率和比浊法检测的血小板最大聚集率(MPAR)均无明显变化;奥美拉唑与泮托拉唑组间比较,差异也无统计学意义。服药15d,30d与服药前1d相比,每组血小板抑制率明显升高,血小板最大聚集率明显下降,差异有统计学意义(P0.05);但15d和30d相比较,差异无统计学意义。②三组比较心血管事件发生率相近,差异无统计学意义(P0.05);奥美拉唑组和泮托拉唑组比较,心血管事件发生率也无统计学差异(P0.05)。③与对照组比较,奥美拉唑组和泮托拉唑组胃肠道出血发生率均明显减少,有统计学意义(P0.05),但两服药组间比较,出血发生率无明显区别,差异无统计学意义(P0.05)。结论:氯吡格雷联合阿司匹林具有增强血小板抑制,降低血小板凝聚的作用,而不同机制质子泵抑制剂奥美拉唑与泮托拉唑对PCI术后氯吡格雷联合阿司匹林抗血小板治疗患者的血小板功能无明显影响,不降低对心血管事件的预防效果,同时明显降低患者胃肠出血事件的发生率。     

不同浓度臭氧水对牙周致病菌的体外抑菌作用

目的 探讨不同浓度臭氧水对4种牙周致病菌的体外抑菌效果。方法 采用定量悬液法,分别用1.02 ppm、2.03 ppm和3.88 ppm三种浓度臭氧水,0 ppm无臭氧水溶液（阴性对照组）及3%双氧水（阳性对照组）,对常见的4种牙周致病菌牙龈卟啉单胞菌（Porphyromonas gingivalis）、具核梭杆菌（Fusobacterium nucleatum）、中间普氏菌（Prevotella intermedia）和黏性放线菌（Actinomyces viscosus）分别作用30 s、60 s后,观察不同作用时间下3种不同浓度臭氧水的抑菌效果。结果 对P. gingivalis、F. nucleatum、P. intermedia和A. viscosus作用30 s和60 s,与阴性对照组比较,1.02 ppm、2.03 ppm和3.88 ppm三种浓度臭氧水均具有明显的抑菌效果,3.88 ppm臭氧水与3%双氧水对4种细菌作用60 s的抑菌效果相同。 结论 实验所用的3种不同浓度臭氧水对牙周致病菌P. gingivalis、F. nucleatum、P. intermedia和A. viscosus均有抑菌作用,浓度越高,抑菌效果越好,臭氧水应用于牙龈牙周疾病临床防治有一定的价值和可行性。