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外源基因在转基因植物中的遗传特性 总被引:13,自引:0,他引:13
GeneticCharacterofForeignGenesinTransgenePlantsWangGuanlinFangHongJunNaJie(BiotechnologyInstituteofLiaoningNormalUniversity,Dalian116029)1外源基因在转化植株中的遗传传递规律关于外源基因在转化植株世代过程中的遗传传递规体已有较多的研究,一般从表型传达、标记基因的表达及分子杂交分析等方面进行研究,Braun等问‘早在1959年已证明,nopaline型Ti质粒农杆菌侵染植物细胞后产生的冠瘿瘤细胞中带有T-DNA,并保留一些形态发生潜力、但只能形成异常穿,亦称畸形痛。通过嫁接到正常烟草植株上可使这些异常穿开花结果… 相似文献
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外源基因在转基因动物中遗传和表达的稳定性 总被引:2,自引:0,他引:2
转基因技术经过近半个世纪的发展,已成为当今生物技术研究的热点。近10多年来,与核移植技术的结合,转基因效率大大提高,携带有不同外源基因的不同种类的转基因动物迅速增加。但是,成功获得转基因动物并不是转基因动物研究的最终目的,如何利用转基因技术为人类的需求服务才是科研人员始终面对的课题。在畜牧生产领域,通过转基因技术培育家畜新品种是转基因技术应用的重要体现,在我国这方面已经引起了广泛关注。但迄今为止,外源基因在转基因动物中遗传和表达的稳定性仍然是亟待解决的问题,究其原因,这主要与位置效应、外源基因的表观遗传学修饰和遗传效率相关,文章结合目前的研究进展和本实验室的研究结果,从这3方面阐述其作用机制,期望为转基因动物遗传育种向产业化的迈进提供一定的理论探讨。 相似文献
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药用植物转基因研究进展 总被引:1,自引:0,他引:1
随着医疗事业的发展,药用植物的遗传转化越来越成为人们关注的焦点.近年来药用植物的遗传转化取得了很大进展,已成功培育了多种转基因药用植物.从遗传转化方法、转化受体和转化的目的基因等方面来论述了近年来药用植物转基因的研究进展,并对以后的发展提出了展望. 相似文献
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安全转基因技术研究进展 总被引:2,自引:1,他引:2
转基因生物安全性问题已引起了公众的普遍担忧,严重制约了转基因技术成果的推广应用。近年来,研究者探索了不同的技术策略来解决转基因生物的安全性问题,安全转基因技术成为当前转基因研究的热点之一。文章介绍并评价了几种主要的安全转基因技术,包括无选择性标记基因技术、安全标记基因技术、叶绿体转化技术、终止子技术、雄性不育技术、外源基因删除技术。其中,外源基因删除技术为实现转基因生物的安全应用展示了诱人的前景。最后,对探索新的安全转基因技术策略保障转基因生物安全提出了建议。 相似文献
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Multiple shoots in Arachis hypogaea L. could be induced from the de-embryonated cotyledons (DC), embryo-axes (EA) and mature whole seeds (MWS) in MS medium supplemented with different levels of benzylaminopurine (BAP). DC was the most suitable explant with 57.9 % induction and more than 40 shoots per explant in 31.6 % of cases. Though EA and MWS had high percent induction at or above 30 mg dm–3 BAP, only 10 – 14 shoots per explant were observed. In DC, multiple shoots were confined to the proximal end and in EA they originated from the axillary bud region. Histological studies on DC confirmed the origin of shoots from the region of attachment with the embryo. Shoots could be rooted in MS medium containing 2 g dm–3 charcoal and 200 mg dm–3 casein hydrolysate. Sixty percent of the rooted plantlets could be established in the field. 相似文献
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不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用 总被引:4,自引:0,他引:4
以85份花生栽培种为材料,分别应用银染法和荧光检测法检测9对SSR引物的扩增产物。比较结果显示,荧光检测法具有灵敏度高、检测结果准确、效率高等优点。聚类分析表明,银染法与荧光检测法分别能够区分74个、82个花生品种,并分别聚成8个、9个类群;荧光检测法的聚类结果虽然反映的品种间遗传多样性较低,但与品种类型、产地及其亲缘关系相关程度更高,表明荧光检测数据更精确、可靠。遗传多样性分析发现,地方品种的遗传多样性指数最高,其次为多粒型育成品种,表明我国地方品种和多粒型育成品种蕴藏了丰富的优异性状,有利于对其挖掘和利用。 相似文献
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Peanut Stripe Potyvirus Resistance in Peanut (Arachis Hypogaea L.) Plants Carrying Viral Coat Protein Gene Sequences 总被引:3,自引:0,他引:3
Peanut (Arachis hypogaea L.) lines exhibiting high levels of resistance to peanut stripe virus (PStV) were obtained following microprojectile bombardment of embryogenic callus derived from mature seeds. Fertile plants of the commercial cultivars Gajah and NC7 were regenerated following co-bombardment with the hygromycin resistance gene and one of two forms of the PStV coat protein (CP) gene, an untranslatable, full length sequence (CP2) or a translatable gene encoding a CP with an N-terminal truncation (CP4). High level resistance to PStV was observed for both transgenes when plants were challenged with the homologous virus isolate. The mechanism of resistance appears to be RNA-mediated, since plants carrying either the untranslatable CP2 or CP4 had no detectable protein expression, but were resistant or immune (no virus replication). Furthermore, highly resistant, but not susceptible CP2 T0 plants contained transgene-specific small RNAs. These plants now provide important germplasm for peanut breeding, particularly in countries where PStV is endemic and poses a major constraint to peanut production. 相似文献
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以花生幼叶为外植体进行离体培养,研究BA浓度对花器官分化的影响并进一步观察试管内花器官的发育.结果表明:经MSB 1mg/LBA 0.5mg/LKIN 2mg/LIAA培养基诱导的愈伤组织,转接到附加1~3mg/LBA的MSB培养基上培养,均能直接诱导分化花器官,但2mg/LBA的诱导效率最高达21.13%;诱导分化的花器官转接到MSB培养基继续培养,部分花器官可以在试管内开花、受精、成针、结实.试验实现了以花生幼叶为外植体,在试管内完成诱导花芽、开花、受精、形成果针、子房膨大,直至形成荚果等过程,为离体条件下研究花生花器官分化、荚果及种子发育提供了技术体系和材料. 相似文献
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AhNCED1基因转化花生研究 总被引:1,自引:0,他引:1
构建转化AhNCED1基因花生(Arachis hypogaea L.)过表达载体35S::AhNCED1::GUS,用OD600=0.8的LBA4404农杆菌液浸染汕油523,抗性芽诱导率达100%.PCR检测89株筛选苗,43株呈阳性,GUS检测阳性率为50%.转基因植株地上部分ABA含量增加;PEG胁迫10 h,转基因植株叶片AhNCEDl蛋白表达增强,内源ABA水平积累,超氧化物水平降低. 相似文献
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花生根部性状的遗传分析 总被引:1,自引:0,他引:1
利用花生RIL群体,分析了11个花生根部性状的遗传力,估算基因对数及性状间的相互关系,根据偏度系数(g1)和峰度系数(g2)估算控制性状的基因互作情况。结果表明:11个性状都是受多基因控制的数量性状,在RIL群体中基因型间的差异均表现为连续变异和明显的超亲分离。侧根干重的遗传力最高达0.60,其次是侧根鲜重,为0.58,而其他性状的遗传力均较低。控制主根长性状的多基因间存在互作,互作方式为重叠作用;控制主根粗(3cm)性状的基因间也存在一定的重叠作用,但是作用不明显;控制其他性状的基因都存在互作,表现为互补作用,但互补作用的强弱有差异。主根粗(1cm)、主根粗(3cm)、主根干重、主根鲜重、侧根干重和侧根鲜重之间都显著或极显著相关;根体积与主根粗(1cm)、主根粗(3cm)、侧根干重和侧根鲜重显著或极显著相关。 相似文献
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Over Expression of Rice chitinase Gene in Transgenic Peanut (Arachis hypogaea L.) Improves Resistance Against Leaf Spot 总被引:1,自引:0,他引:1
A Rice chitinase-3 under enhance version of CaMV 35S was introduced into peanut (Arachis hypogaea L.) through Agrobacterium mediation. Agrobacterium tumefaciens strain LB4404 was used harboring the binary vector (pB1333-EN4-RCG3) containing the chitinase (chit) and hygromycin resistance (hpt) gene as selectable marker. Putative transgenic shoots were regenerated and grown on MS medium supplemented with 5 mg/l BAP,
1 mg/l kinetin, and 30 mg/l hygromycin. Elongated shoots were examined for the presence of the integrated rice chitinase gene along with hygromycin gene as selectable. The integration pattern of transgene in the nuclear genome of the putative
transformed plants (T0) was confirmed through Southern hybridization analysis of the genomic DNA. Survival rate of the in vitro regenerated plantlets
was over 60% while healthy putatively transgenic (T0) plants with over 42% transformation frequency were produced through Agrobacterium mediated gene transfer of the rice chitinase gene and all the plants flowered and set seed normally. T1 plants were tested for resistance against Cercospora arachidicola by infection with the microspores. Transgenic strains exhibited a higher resistance than the control (non-transgenic plants).
chitinase gene expression in highly resistant transgenic strains was compared to that of a susceptible control. A good correlation
was observed between chitinase activity and fungal pathogen resistance. 相似文献