共查询到20条相似文献,搜索用时 46 毫秒
1.
本文对混合单克隆抗体ELISA直接法和ELISA夹心法检测包虫病人循环抗原的效果进行了比较研究。结果表明,ELISA夹心法对细粒棘球蚴囊液纯化抗原的最低检出量(1.2ng/ml)较直接法(10ng/ml)低,但检测包虫病人循环抗原时,ELISA直接法的阳性检出率为56.39%(27/48),ELISA夹心法为60.4%(29/48),二者无显著性差异(P>0.05)。两法同时检测14份猪囊尾蚴病人血清和44份正常人血清,均未出现假阳性反应。 相似文献
2.
清洁级实验动物四种病毒抗体玻片酶免疫检测试剂盒的研制 总被引:1,自引:0,他引:1
本文报道对清洁级实验动物应排除的四种病毒(淋巴细胞脉络丛脑膜炎病毒、小鼠脱脚病病毒、鼠肝炎病毒和仙台病毒)抗体玻片酶免疫(EIA)检测试剂盒的研制。四种病毒感染的细胞和对照细胞经冷丙酮固定于载玻片上制成特异性抗原和对照抗原,此四种病毒的抗血清各10份和SPF小鼠血清20份分别与四种病毒的特异性抗原和对照抗原进行EIA交叉试验,结果显示,抗原只与其相应抗血清发生特异性显色反应,与非特异性小鼠血清和SPF小鼠血清不显色。与HI或ELISA方法比较,通过对112份普通小鼠血清进行测试,结果表明,EIA对仙台病毒抗体的检出率(19.6%)显著高于(<0.005)HI(6.3%),对小鼠脱脚病病毒抗体的检出率(23.3%)与HI(21.4%)无显著性差异(P>0.05)。EIA对淋巴细胞脉络丛脑膜炎病毒和鼠肝炎病毒抗体的检出率分别为1.8%和71.2%,ELISA对两种病毒抗体的检出率分别为1.8%和67.6%,两种方法对两种病毒抗体的检出率无显著性差异(P>0.05)。重复性试验表明两批四种病毒抗体试剂盒对108份小鼠血清两次测定的符合率为96~100%。四种病毒的EIA抗原在-18℃保存12个月或在2-8℃保存3 相似文献
3.
丙型肝炎病毒核心蛋白基因在大肠杆菌内的表达及应用 总被引:2,自引:0,他引:2
将从中国丙肝病人血清中扩增克隆的丙型肝炎病毒核心蛋白基因(408bp)酶切处理后插入表达载体pJLA502内,获得高表达核心蛋白的重组工程菌。将重组菌经42℃热诱导5h,SDS-PAGE分析表明,表达的核心蛋白占菌体蛋白总量的20%。经分子筛和吸附层析纯化后获得的核心蛋白,ELISA检测证实有较好的抗原性和特异性。用表达的核心抗原加用表达的NS_3抗原(C_33)装配的抗-HCV试剂盒,经用标准血清验证及与国外第二代抗-HCV试剂盒比较,证实符合丙肝诊断试剂要求。 相似文献
4.
鼠颌下腺提纯的25SNGF免疫家兔,获得兔抗NGF抗体,研制出鼠25SNGFELISA检测试剂盒,该试剂盒灵敏度小于1ng/ml,在670~084ng/ml范围内,线性良好,r=099。与大鼠、小鼠及人血浆无非特异反应,在大鼠血浆中,NGF样品回收率在91%~107%之间,变异系数小于10%(n=4)。结果表明:本试剂盒操作简便,灵敏度高,特异性强,适合药代动力学研究及生产过程中的NGF检测。 相似文献
5.
小鼠肝炎病毒抗体检测试剂盒的标准化研究 总被引:2,自引:0,他引:2
目的筛选适合制备检测试剂盒的抗原组合。方法用 ELISA方法,将5株小鼠肝炎病毒(MHV)抗原分别组合作为包被抗原,研究MHV抗原不同组合与标准毒株抗体的反应性,分析MHV各毒株间的差别,进一步比较不同组台抗原的灵敏性、特异性。结果通过对1997~1999年送检238只普通级实验小鼠检测,结果表明,MHV的感染率虽有下降趋势,但感染率仍然很高(59%~87%)。结论我国分离的MHV-HU79株,在本次研究中表明能够用于MHV抗体检测试剂盒。 相似文献
6.
应用杂交瘤技术,以A型红细胞,A1血型物质MSM(A1)和A-RBC+MSM(A1)为免疫原,制备了一组抗人A血型单克隆抗体:A1218,B57,DE923-G8,D286-E12经Takatsy微量血细胞凝集试验证明:这组单抗仅能凝集A1,A2及AB型红细胞,不能凝集B,O型红细胞。采用ELISA定量抑制试验法,精确测定了它们抗原结合部位的结构,互补于A活性寡糖。A1218互补于具有双岩藻糖结构 相似文献
7.
对国内6个厂家28批AFP ELISA诊断试剂盒的精密性、灵敏度、特异性、线性和盒内AFP工作标准品的准确性进行全面质量的考查。结果表明目前我国大多数AFP ELISA诊断试剂盒的灵敏度均能达到≤5IU/ml,精密性(孔间差)为15%~20%;线性r值≥098;特异性良好。但存在的主要问题是盒内AFP工作标准品的标示量与AFP国家标准品标示量不符。不符合率占428%。 相似文献
8.
厚果鸡血藤凝集素的纯化及性质 总被引:2,自引:0,他引:2
从厚果鸡血藤(MiletiapachycarpaBenth.)的种子中分离纯化出一种具强凝集活性和强促有丝分裂原的凝集素。种子经磨粉、浸取、硫酸铵分级、DEAESepharose离子交换和SephadexG100分子筛层析,即可获得在PAGE和SDSPAGE上均呈现单一蛋白染色带的凝集素纯品,分子筛层析测得分子量为40700,SDSPAGE测得亚基分子量为19800;含有178%的中性糖。氨基酸组成分析表明,该凝集素富含Asp、Glu、Thr、Ser和Leu,同时含有4个Trp,当凝集素浓度为0.48μg/mL时,即可凝集兔红细胞;对人A、B和O型血细胞都能发生凝集,故无血型专一性;其凝集兔红细胞的凝集活性,不能被常见糖类抑制,但可被甲状腺球蛋白、胃粘蛋白和卵粘蛋白所抑制;其凝集活性强烈地依赖于Ca2+的存在,但Mg2+、Mn2+、Zn2+对其凝集活性全无促进作用;该凝集素是一种强促有丝分裂原,对人外围血中淋巴细胞的转化率高达843%,细胞分裂比率可达78%。 相似文献
9.
应用杂交瘤技术,以A型红细胞,A1血型物质MSM(A1)和A-RBC+MSM(A1)为免疫原,制备了一组抗人A血型单克隆抗体:A1218,B57,DE923-G8,D286-E12经Takatsy微量血细胞凝集试验证明:这组单抗仅能凝集A1,A2及AB型红细胞,不能凝集B,O型红细胞.采用ELISA定量抑制试验法,精确测定了它们抗原结合部位的结构,互补于A活性寡糖。A1218互补于具有双岩藻糖结构的A活性五糖(A-Penta);B57,DE923-G8互补于具有单岩藻糖结构的A活性六糖(A-Hexa);而D286-E12则互补于具有单岩藻糖的A活性四糖(A-Tetra).结果表明:血凝特异性相同的抗A单抗,其抗原结合部位的结构可呈现多样性。即A活性寡糖的糖基组成数目和含有岩藻糖数目均可不相同,各种抑制剂对不同单抗的抑制作用强弱也不相同。 相似文献
10.
用聚合酶链式反应(PCR)从HIV 1gag基因中扩增出衣壳蛋白P24截短体(tP24)基因,插入质粒pGEX 4T3中构成重组表达质粒pGEX tp24,将pGEX tp24转化大肠杆菌BL21后获得了高效表达,表达量占菌体总蛋白量的3438%。经Glutathione-Sepharose4B亲和层析纯化的截短体P24纯度为9277%。纯化的截短体P24在间接ELISA和免疫印迹检测HIV抗体阳性血清和正常人血清中,具有很高的抗原特异性和免疫反应性。 相似文献
11.
A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability. 相似文献
12.
13.
Summary Concentration of N, P, K, Ca, Mg and S in summer groundnut crop was higher than in kharif while Zn, Fe, Mn and Cu contents were higher in summer crop. Kernel's N, P and Zn; Leaflet's Ca and Mn; Stem's K and Fe; Root's S and Cu and Petiole's Mg contents were highest. Shell's N, P, K, Mg, S, Zn and Cu; Kernel's Ca, Fe and Mn contents were the least. N, P, K, S, Zn and Cu concentrations decreased linearly as the crop grew. Ca, Mg, Fe and Mn concentrations did not display any distinct pattern. Ca concentration was positively correlated with pod yield in both the seasons. 相似文献
14.
The variation with age of the 18 trace element mass fractions and some histological characteristics of intact prostate glands of 50 subjects aged 0–30 years was investigated by instrumental neutron activation analysis, inductively coupled plasma atomic emission spectrometry, and a quantitative morphometric analysis. Mean values ± standard error of the mean (M ± SΕΜ) for the mass fractions (in milligrams per kilogram wet tissue) of these trace elements in pre-puberty were: Al 28.5 ± 9.0, B 0.40 ± 0.11, Ba 1.48 ± 0.44, Br 10.5 ± 1.5, Ca 241 ± 30, Cl 3,203 ± 278, Cu 3.51 ± 0.89, Fe 33.7 ± 4.1, K 2,364 ± 145, Li 0.020 ± 0.004, Mg 153 ± 23, Mn 0.46 ± 0.06, Na 2,286 ± 130, P 1,391 ± 100, S 1,698 ± 132, Si 62 ± 11, Sr 0.38 ± 0.08, and Zn 27.6 ± 2.3. During puberty and postpuberty, when there is a significant increase in circulating androgens, the mean values were: Al 7.2 ± 1.4, B 0.21 ± 0.05, Ba 0.25 ± 0.06, Br 5.8 ± 1.0, Ca 433 ± 81, Cl 2,314 ± 201, Cu 1.77 ± 0.13, Fe 20.9 ± 1.6, K 2,585 ± 118, Li 0.0088 ± 0.0014, Mg 232 ± 27, Mn 0.34 ± 0.04, Na 1,875 ± 107, P 1,403 ± 98, S 1,673 ± 73, Si 22.2 ± 3.1, Sr 0.22 ± 0.03, and Zn 93.3 ± 8.9. Mean values (M ± SΕΜ) of percent volumes (%) of the stroma, epithelium and lumen in the prostate before puberty were 73.4 ± 2.6, 20.4 ± 1.7, and 4.45 ± 0.94, respectively, versus 46.5 ± 2.5, 38.5 ± 1.9, and 14.9 ± 1.2 during puberty and postpuberty. This work’s results confirm that the Zn mass fraction in prostate tissue is an androgen-dependent parameter. For the first time it has been demonstrated that the glandular lumen is a main pool of Ca, Mg, and Zn accumulation and that the stroma is a main pool of Al, B, Ba, Br, Cl, Cu, Fe, Mn, Na, and Si accumulation in the normal human prostate, for the age range 0–30 years. It was concluded that the Ca, Mg, and Zn binds tightly within the prostatic fluid, because the volume of glandular lumen reflects the volume of prostatic fluid. 相似文献
15.
The goal of this article was to establish reference ranges of the concentration of trace elements in human serum and to compare
these results with those reported by other authors. We describe the sample preparation and measurement conditions that allow
the rapid, precise, and accurate determination of Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human
serum samples (n=110) by inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy and precision were determined by analyzing three
reconstituted reference serum samples by comparison with other methods and by the standard addition procedure.
The advantages of the ICP-MS method include short time of analysis of the elements mentioned, low detection limit, high precision,
and high accuracy. Disadventages include a high risk of contamination due to the presence of some of the elements of interest
in the environment, the relatively delicate sample handling, and the high cost of the equipment. 相似文献
16.
G. V. Iyengar 《Biological trace element research》1987,12(1):263-295
This report attempts to formulate reference ranges of elemental concentrations for 15 trace elements in selected human tissues
and body fluids.
A set of samples consisting of whole blood, blood serum, urine, milk, liver, and hair were chosen and considered for 15 elements
of biological significance: As, Cd, Co, Cr, Cu, F, Fe, I, Hg, Mn, Mo, Ni, Pb, Se, and Zn. The results represent wholly or
partially data received from 40 countries of the global regions of Africa, Asia, Europe, North, South, and Central America,
Australia, and New Zealand.
This survey, even if qualitative, has been useful in demonstrating certain trends of trace-element scenarios around the world.
It is of course recognized that both diet and environment exert a strong influence on the distribution pattern of several
elements, such as As, Cd, Mn, Pb, Se, and Zn. A limited comparison of the available information on soil status of different
countries reflected some interesting associations for elements, such as Mn and Zn.
Importantly, this study revealed that only a few countries were in a position to identify a reasonable amount of data on samples
requested for this project. Regretably, for a number of countries, any dependable data for even such essential elements as
Cu, Fe, and Zn were not available.
In view of the nutritional importance of many elements, the time is ripe for concerted efforts by intergovernmental agencies
to initiate investigations or commission task forces/projects to generate reliable reference data for selected global regions,
which sadly lack data of any kind at present. 相似文献
17.
Vladimir Zaichick Sofia Zaichick Vasilii Karandashev Sergey Nosenko 《Biological trace element research》2009,129(1-3):107-115
The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M?±?SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54?±?0.16, 171,400?±?4,050, 1.35?±?0.22, 140?±?11, 1,874?±?71, 0.049?±?0.011, 2,139?±?38, 5,378?±?88, 75,140?±?1,660, 1,881?±?51, 291?±?20, and 92.8?±?1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs. 相似文献
18.
19.
20.
Oral cancer is one of the main causes of cancer-related deaths in South-Asian countries. There are very limited treatment options available for oral cancer. Research endeavors focused on discovery and development of novel therapies for oral cancer, is necessary to control the ever rising oral cancer related mortalities. We mined the large pool of compounds from the publicly available compound databases, to identify potential therapeutic compounds for oral cancer. Over 84 million compounds were screened for the possible anti-cancer activity by custom build SVM classifier. The molecular targets of the predicted anti-cancer compounds were mined from reliable sources like experimental bioassays studies associated with the compound, and from protein-compound interaction databases. Therapeutic compounds from DrugBank, and a list of natural anti-cancer compounds derived from literature mining of published studies, were used for building partial least squares regression model. The regression model thus built, was used for the estimation of oral cancer specific weights based on the molecular targets. These weights were used to compute scores for screening the predicted anti-cancer compounds for their potential to treat oral cancer. The list of potential compounds was annotated with corresponding physicochemical properties, cancer specific bioactivity evidences, and literature evidences. In all, 288 compounds with the potential to treat oral cancer were identified in the current study. The majority of the compounds in this list are natural products, which are well-tolerated and have minimal side-effects compared to the synthetic counterparts. Some of the potential therapeutic compounds identified in the current study are resveratrol, nimbolide, lovastatin, bortezomib, vorinostat, berberine, pterostilbene, deguelin, andrographolide, and colchicine. 相似文献