共查询到19条相似文献,搜索用时 156 毫秒
1.
在低pH静脉注射用人免疫球蛋白(IVIG)的制备中,采用有机溶剂/表面活性剂处理和低pH放孵(23°C~25°C,21天)进行病毒灭活,以提高IVIG的安全性,两法累积灭活效果为:>8logHIV-Ⅰ;>11.3LogVSV和>10.8LogSindbis。 相似文献
2.
以水泡性口炎病毒(VSV)为指示病毒,考察了低pH孵放不同时间对低温乙醇法生产的静注丙球(IVIG)中VSV的灭活效果,并对不同厂家及不同批号IVIG中病毒灭活情况进行了比较。结果表明,液体IVIG在PH4.1±0.3,20-25℃,孵放21天可灭活VSV达6Logs以上(即低于实验检测限),但不同厂家及不同批号的IVIG在病毒灭活的发生上有所不同 相似文献
3.
静脉注射免疫球蛋白制备中的病毒灭活 总被引:1,自引:0,他引:1
在静脉注射免疫球蛋白(IVIG)的制备中,采用有机溶剂结合表面活性剂(S/D)处理或60℃10小时液态加热(巴氏灭活法)的方法对组分Ⅱ(IgG)进行病毒灭活处理,灭活效果用指示病毒进行了评价,结果表明:S/D法可有效灭活脂包膜病毒VSV和Sindbis,巴氏灭活法则对上述病毒以及Vaccinia和Echo病毒均有较好的灭活作用。经病毒灭活处理的免疫球蛋白,在理化及生物学特性上基本未受到不利影响,用两种方法分别处理组分Ⅱ后制备的IVIG,其主要特性指标均符合该制品的有关质量规定。 相似文献
4.
将含有鸡传染性支气管炎病毒 S1 基因c D N A 的重组转移质粒p S X I V V I+ X3 S1 . Holte 和p S X I V V I+ X3/4 S1 . Holte 分别与粉纹夜蛾核型多角体病毒 Tn N P V S V I- G D N A( O C C- ,gal+ ) 共转染草地夜蛾( Sf9) 细胞,经空斑纯化得到重组病毒 Tn N P V( X3) S1 . Holte O C C+ 和 Tn N P V( X3/4) S1 . Holte O C C+ 。将重组毒株分别感染 Tn5 B1 细胞,并进行 S D S P A G E 与 Westernblot 检测。结果表明, Tn N P V( X3/4) S1 . Holte O C C+ 在感染的细胞中高效表达了 S1 蛋白, S D S P A G E 凝胶薄层色谱分析结果显示,感染病毒后72 h S1 蛋白的表达量占细胞内总蛋白量的35 .8 % ,而 Tn N P V( X3) S1 . Holte O C C+ 感染的细胞内检测不出 S1 蛋白。经分析认为这一差异主要来自 S1 基因翻译起始位点及其附近的周围环境。 相似文献
5.
稀土La~(3+)和维生素C对黄瓜PSⅡ颗粒放氧活性机理影响的初探 总被引:1,自引:1,他引:0
用10μg/gLa ̄(3+)、1.0%Vc、10μg/gLa ̄(3+)+1·0%Vc、蒸馏水四种处理,喷施正处在衰老过程中的黄瓜叶片,一周后提取PSⅡ颗粒,通过SDS-PAGE、薄层扫描和Clark测氧等测试技术,发现处理与对照的PSⅡ颗粒在与放氧活性密切相关的33、23、17kD三肽的相对总含量上有变化,10μg/gLa ̄(3+)处理比对照减少28.9%,而1.0%Vc和10μg/gLa ̄(3+)+1.0%Vc处理的与对照接近。前者的放氧活性降低37.3%,后者的放氧活性增加62.4%,是对照的1.6倍,共同处理的结果与对照相近。可见,在光合放氧中,1.0%Vc与10μg/gLa ̄(3+)拮抗。而且,33、23、17kD三肽总量的相对减少与放氧活性的高低呈正相关。 相似文献
6.
用聚丙烯酰胺电泳分离并测定了大鼠左室肌凝蛋白ATP酶活性依次降低的同功酶V_1,V_2和V_3的百分比(MI谱),从乳头肌力-速度曲线读取心肌最大缩短速度(V_(max)),观察到:(1)正常大鼠出现增龄性V_1向V_3迁移和V_(max)下降,与8周龄组(S_0)相比,16周和24周龄组(S_8和S_(16))的V_1/V_3比,分别下降38.9%和61.0%;V_(max)下降8.3%和13.3%。(2)高血压肥厚心肌MI谱的迁移和V_(max)下降的程度大大超过增龄效应:高血压8周和16周组(H_8和H_(16))的V_1/V_3比值较术前对照S_0分别下降84.4%和93.5%,较同龄假手术对照S_8和S_(16)组也分别低74.5%和83.3%,而V_(max)则比S_0组下降33.3%和48.3%。(3)6组48只大鼠结果相关分析表明,V_(max)与V_1%高度正相关,与V_3%高度负相关。(P均小于0.01)。上述结果提示:高血压肥厚心肌收缩速度明显下降,其主要生化机制似与同功酶谱由V_1优势向V_3迁移有关。 相似文献
7.
用黄瓜花叶病毒卫星RNA生防制剂大面积防治麦茬辣椒病毒病 总被引:1,自引:0,他引:1
用黄瓜花叶病毒(CMV)卫星RNA生防制剂S_(52)免疫辣椒,能防治辣椒由CMV引起的病毒病。用S_(52)免疫接种辣椒的大田对比试验,防效达59.5-71.0%,产量比对照增加26.1-32.2%,产值增加33.5-45.2%。小区对比试验,用S_(52)免疫接种50天的辣椒,防效达71.3-92.9%,免疫接种80天,防效达55.9-78.5%,产量比对照增加36.0-65.3%,产值增加49.3-93.5%,还有刺激生长,促进早熟和增强对真菌、细菌病害抵抗作用。 相似文献
8.
黑龙江省及长春市烟草病毒病的种类鉴定 总被引:4,自引:0,他引:4
1991-1993年在黑龙江省主要烟区的11个县及长春市采样,得129个毒株。经鉴别寄主测定,抗血清反应(板式酶联法或斑点酶联法)及电镜或免疫电镜观察,有TMV(43.4%),PVY(17.8%),CMV(3.9%),TRV(0.8%),TSWV等病毒,TMV与PVY混合侵染的占10.1%,PVY与其它病毒混合侵染的占11.6%,另有5个标样为马铃薯Y病毒组成员,10个为未知。 相似文献
9.
减蛋综合征病毒100K蛋白基因的克隆与序列分析 总被引:2,自引:0,他引:2
用常规方法提取减蛋综合征病毒(EDSV)中国分离株(AA2株)病毒DNA,分别构建了限制性内切酶HindⅢ、SphⅠ、PstⅠ水解片段的全基因文库,并对其中100K蛋白基因的序列进行了分析。EDSV100K蛋白基因位于减蛋综合征病毒基因组55.7~64.8物理图谱单位(m.u),共2091个核苷酸(nt),其编码产物由696个氨基酸(aa)组成,推测其分子量为77.7kD。编码蛋白氨基酸同源性分析表明,EDSV100K蛋白与人腺病毒(Ad2、Ad5、Ad12、Ad41)、Ⅰ群禽腺病毒(CELO和FAV10)的同源性为32.3~34.4%之间,而与羊腺病毒(OAV)的同源性达到56.4%。 相似文献
10.
用BamHI和HindII将丙肝病毒C+E1DNA片段从其克隆载体pGEM3zf-HCV/C+E1上切下,经Taq酶补齐3’末端后插入到载体pSVL-T中,构建成丙肝病毒C+E1真核表达载体pSVL-HCV/C+E1。本实验中重组效率达64.7%(11/17),正向插入为50%(2/4)。 相似文献
11.
Weimin Zhou Feng Lin Lingfang Teng Hua Li Jianyi Hou Rui Tong Changhua Zheng Yongliang Lou Wenjie Tan 《PloS one》2013,8(11)
Objective
Few comprehensive studies have searched for viruses in infants and young children with community-acquired pneumonia (CAP) in China. The aim of this study was to investigate the roles of human herpes viruses (HHVs) and other respiratory viruses in CAP not caused by typical bacterial infection and to determine their prevalence and clinical significance.Methods
Induced sputum (IS) samples were collected from 354 hospitalised patients (infants, n = 205; children, n = 149) with respiratory illness (CAP or non-CAP) admitted to Wenling Hospital of China. We tested for HHVs and respiratory viruses using PCR-based assays. The epidemiological profiles were also analysed.Results
High rate of virus detection (more than 98%) and co-infection (more than 80%) were found among IS samples from 354 hospitalised infants and children with respiratory illness in this study. Of 273 CAP samples tested, CMV (91.6%), HHV-6 (50.9%), RSV (37.4%), EBV (35.5%), HBoV (28.2%), HHV-7 (18.3%) and rhinovirus (17.2%) were the most commonly detected viruses. Of 81 non- CAP samples tested, CMV (63%), RSV (49.4%), HHV-6 (42%), EBV (24.7%), HHV-7 (13.6%) and HBoV (8.6%) were the dominant viruses detected. The prevalence of several viral agents (rhinovirus, bocavirus, adenovirus and CMV) among IS samples of CAP were significantly higher than that of non-CAP control group. We also found the prevalence of RSV coinfection with HHVs was also higher among CAP group than that of non-CAP control.Conclusions
With sensitive molecular detection techniques and IS samples, high rates of viral identification were achieved in infants and young children with respiratory illness in a rural area of China. The clinical significance of rhinovirus, bocavirus, adenovirus and HHV (especially CMV) infections should receive greater attention in future treatment and prevention studies of CAP in infants and children. 相似文献12.
Background
Upper respiratory tract infection (URTI) is a major reason for hospitalization in childhood. More than 80% of URTIs are viral. Etiological diagnosis of URTIs is important to make correct clinical decisions on treatment methods. However, data for viral spectrum of URTIs are very limited in Shanghai children.Methods
Nasopharyngeal swabs were collected from a group of 164 children aged below 3 years who were hospitalized due to acute respiratory infection from May 2009 to July 2010 in Shanghai. A VRDAL multiplex PCR for 10 common respiratory viruses was performed on collected specimens compared with the Seeplex® RV15 ACE Detection kit for 15 respiratory viruses.Results
Viruses were detected in 84 (51.2%) patients by VRDAL multiplex PCR, and 8 (4.9%) of cases were mixed infections. Using the Seeplex® RV15 ACE Detection kit, viruses were detected in 129 (78.7%) patients, 49 (29.9%) were co-infected cases. Identified viruses included 37 of human rhinovirus (22.6% of cases), 32 of influenza A virus (19.5%), 30 of parainfluenzavirus-2 (18.3%), 23 of parainfluenzavirus-3 (14.0%), 15 of human enterovirus (9.1%), 14 each of parainfluenzavirus-1, respiratory syncytial virus B and adenovirus (8.5%), 8 of coronavirus 229E/NL63 (4.9%), 6 of human bocavirus (3.7%), 5 each of influenza B virus and respiratory syncytial virus A (3.0%), 3 of parainfluenzavirus-4 (1.8%), 2 of coronavirus OC43/HKU1 (1.2%), and 1 human metapneumovirus (0.6%).Conclusions
A high frequency of respiratory infections (78.7%) and co-infections (29.9%) was detected in children with acute respiratory infection symptoms in Shanghai. The Seeplex® RV15 ACE detection method was found to be a more reliable high throughput tool than VRDAL method to simultaneously detect multiple respiratory viruses. 相似文献13.
This study investigated the relationship between physicochemical properties (namely halogen content and hydrophobicity) of halogenated trace organics and their removal efficiencies by a laboratory scale membrane bioreactor (MBR) under stable operating conditions. The reported results demonstrated a combined effect of halogen content and hydrophobicity on the removal. Compounds with high halogen content (>0.3) were well removed (>85%) when they possessed high hydrophobicity (Log D>3.2), while those with lower Log D values were also well removed if they had low halogen content (<0.1). General indices such as the BIOWIN index (which is based on only biodegradation) or a more specific index such as the halogen content (which captures a chemical aspect) appeared insufficient to predict the removal efficiency of halogenated compounds in MBR. Experimental data confirmed that the ratio of halogen content and Log D, which incorporates two important physico-chemical properties, is comparatively more suitable. 相似文献
14.
Aim
Oral treatment with asunaprevir and daclatasvir has been reported to yield a SVR ratio of 80% in patients with genotype 1b HCV infection, however, treatment failure has been reported, especially in patients with HCV strains showing the NS5A-Y93H mutation at baseline. An assay system to detect such strains was established to facilitate selection of appropriate candidates for this antiviral therapy.Methods
Primer sets and 2 types of cycling probe mixtures were designed, and real-time PCR was performed with HCV-RNA purified from 332 patients with genotype 1b HCV infection, and the results were compared with those obtained by direct sequencing.Results
Both the wild-type and mutant strains were quantified, with a threshold of 4.0 Log copies/mL, in 295 of the 332 patients (88.9%), and the percentage of the mutant strains relative to the total HCV-RNA level in the serum was calculated. The percentage was 0% in 237 patients (80.3%) and 100% in 23 patients (7.8%), identical to the results of direct sequencing. Both wild-type and mutant strains were detected in the remaining 35 patients (11.9%), at levels between 1% and 99%, despite the mutant strains having been undetectable by direct sequencing in 11 patients with percentages of these strains of less than 25%.Conclusion
A novel assay system to quantify the percent RNA of Y93H mutant strains relative to the total HCV-RNA level was established. This system may be useful to determine the indication for treatment with NA5A inhibitors in patients with HCV. 相似文献15.
Inactivation of lipid enveloped viruses by octanoic Acid treatment of immunoglobulin solution. 总被引:2,自引:0,他引:2
Inactivation of lipid enveloped viruses by treatment with octanoic acid has been investigated for three intravenous immunoglobulin preparations, using Human Immunodeficiency Virus, Bovine Viral Diarrhoea Virus, Sindbis Virus and Pseudorabies Virus as test viruses. At a concentration of 7.45 g octanoic acid per kg solution complete inactivation of lipid enveloped viruses to below detectable level (>5.36, >4.68, >6.25 and >5.55 log(10), respectively) was achieved within the first minutes of treatment. Octanoic acid treatment as described here, has been demonstrated as an effective and rapid virus inactivation procedure, which shows high robustness at the tested ranges of temperature, pH and protein content of the test material. However, pH must be considered as a critical parameter of treatment, as octanoic acid fails to inactivate lipid coated viruses at basic pH. At suitable conditions, e.g. pH<6.0 and a concentration of >3.7 g/kg, octanoic acid treatment gives reliable and highly effective inactivation of lipid enveloped viruses. 相似文献
16.
Bagnato F Clemenzi A Scagnolari C Strizzi L Di Pasquale A Bellomi F Di Marco P Antonelli G Antonini G 《European cytokine network》2004,15(1):24-29
Anti-cytokine antibodies (Abs) play an important role in the regulation of the immune response, both under normal conditions and in several autoimmune and neoplastic disorders. In the present study, we have investigated the occurrence and the clinical significance of natural neutralizing Abs (NAbs) against interferons (IFNs) alpha, beta, and gamma, as detected by bioassay, in 52 patients with myasthenia gravis (MG), and 43 sex- and age-matched healthy individuals. Patients showing titres > or = 1.3 Log t(1/10), confirmed in 2 consecutive samples collected two months apart, were considered positive. NAbs against any of the IFNs were not detected in healthy subjects. Of the 52 MG patients, 11 (21.1%) had NAbs against IFNalpha and three (5.8%) had NAbs against IFNbeta. None of these patients was found to be positive for NAbs against IFNgamma. Of the patients positive for NAbs against IFNalpha, eight (15.4%) had NAbs at titres > or =2 Log t(1/10). A positive association was observed between high titres of NAbs and the presence of thymoma. These data suggest the presence of a generalized activation of the humoral response in MG. 相似文献
17.
Hye-Sung Jeong Jin-Ho Shin Jung-Yun Choi Young-Lim Kim Jei-Jun Bae Byoung-Guk Kim Seung-Rel Ryu Soon-Nam Kim Hong-Ki Min Hong-Jin Kim Sue-Nie Park 《Biologicals》2006,34(4):273-279
Biopharmaceutical products produced from cell cultures have a potential for viral contamination from cell sources or from adventitious introduction during production. The objective of this study was to assess viral clearance in the production of insect cell-derived recombinant human papillomavirus (HPV)-16 type L1 virus-like particles (VLPs). We selected Japanese encephalitis virus (JEV), bovine viral diarrhea virus (BVDV), and minute virus of mice (MVM) as relevant viruses to achieve the aim of this study. A downstream process for the production of purified HPV-16 L1 VLPs consisted of detergent lysis of harvested cells, sonication, sucrose cushion centrifugation, and cesium chloride (CsCl) equilibrium density centrifugation. The capacity of each purification/treatment step to clear viruses was expressed as reduction factor by measuring the difference in log virus infectivity of sample pools before and after each process. As a result, detergent treatment (0.5% v/v, Nonidet P-40/phosphate-buffered saline) was effective for inactivating enveloped viruses such as JEV and BVDV, but no significant reduction (< 1.0 log(10)) was observed in the non-enveloped MVM. The CsCl equilibrium density centrifugation was fairly effective for separating all three relevant adventitious viruses with different CsCl buoyant density from that of HPV-16 L1 VLPs (JEV, BVDV, and MVM = 4.30, 3.10, > or = 4.40 log(10) reductions). Given the study conditions we used, overall cumulative reduction factors for clearance of JEV, BVDV, and MVM were > or = 10.50, > or = 9.20, and > or = 6.40 log(10) in 150 ml of starting cell cultures, respectively. 相似文献
18.
Cecilia Waruhiu Sheila Ommeh Vincent Obanda Bernard Agwanda Francis Gakuya Xing-Yi Ge Xing-Lou Yang Li-Jun Wu Ali Zohaib Ben Hu Zheng-Li Shi 《中国病毒学》2017,32(2):101-114
This is the first country-wide surveillance of bat-borne viruses in Kenya spanning from 2012–2015covering sites perceived to have medium to high level bat-human interaction. The objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of Kenyan bats. We screened for both DNA and RNA viruses;specifically, astroviruses(AstVs), adenoviruses(ADVs), caliciviruses(CalVs), coronaviruses(CoVs), flaviviruses, filoviruses, paramyxoviruses(PMVs), polyomaviruses(PYVs) and rotaviruses.We used family-specific primers, amplicon sequencing and further characterization by phylogenetic analysis. Except for filoviruses, eight virus families were detected with varying distributions and positive rates across the five regions(former provinces) studied. AstVs(12.83%), CoVs(3.97%), PMV(2.4%), ADV(2.26%), PYV(1.65%), CalVs(0.29%), rotavirus(0.19%) and flavivirus(0.19%). Novel CalVs were detected in Rousettus aegyptiacus and Mops condylurus while novel Rotavirus-A-related viruses were detected in Taphozous bats and R. aegyptiacus. The two Rotavirus A(RVA) strains detected were highly related to human strains with VP6 genotypes I2 and I16. Genotype I16 has previously been assigned to human RVA-strain B10 from Kenya only, which raises public health concern, particularly considering increased human-bat interaction.Additionally, 229E-like bat CoVs were detected in samples originating from Hipposideros bats roosting in sites with high human activity. Our findings confirm the presence of diverse viruses in Kenyan bats while providing extended knowledge on bat virus distribution. The detection of viruses highly related to human strains and hence of public health concern, underscores the importance of continuous surveillance. 相似文献
19.
Kim IS Choi YW Kang Y Sung HM Shin JS 《Journal of microbiology and biotechnology》2008,18(5):997-1003
Viral safety is a prerequisite for manufacturing clinical antihemophilic factor VIII concentrates from human plasma. With particular regard to the hepatitis A virus (HAV), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor VIII concentrate. The loss of factor VIII activity during dry-heat treatment was of about 5%. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor VIII compared with those of the factor VIII before dry-heat treatment. The dry-heat-treated factor VIII was stable for up to 24 months at 4oC. The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV, murine encephalomyocarditis virus (EMCV), and human immunodeficiency virus (HIV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Bovine herpes virus (BHV) and bovine viral diarrhea virus (BVDV) were potentially sensitive to the treatment. However porcine parvovirus (PPV) was slightly resistant to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were > or =5.55 for HAV, > or =5.87 for EMCV, > or =5.15 for HIV, 6.13 for BHV, 4.46 for BVDV, and 1.90 for PPV. These results indicate that dry-heat treatment improves the virus safety of factor VIII concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid-enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment. 相似文献