共查询到20条相似文献,搜索用时 546 毫秒
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糖基化是一种十分重要且独特的天然产物结构修饰方式,该修饰由天然产物糖基转移酶(natural product glycosyltransferases, NP-GTs)催化完成,可以改变底物的理化性质或生物学活性。本文首先从结构、催化机制与糖基化位点的角度总结了NP-GTs的分类;其次,从生物来源和底物类别两个角度分析了NPGTs的进化关系;介绍了NP-GTs底物预测的三种常用策略;最后,展望了NP-GTs的研究趋势,为开展基于糖基化修饰的先导化合物优化、蛋白质设计、合成生物学等研究提供参考。 相似文献
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A J Daugulis 《Current opinion in biotechnology》1991,2(3):408-412
As continues to be demonstrated, the in situ recovery of selected products froma bioreactor can have a significant positive impact on production. The strategies that are focused on here are: aqueous two-phase biocatalysis; non-aqueous biocatalysis; and membrane-enhanced biocatalysis. Additional fundamental understanding of molecular partitioning and biocatalytic activity in these environments will facilitate the rational selection of the components involved in these processing strategies. 相似文献
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Kircher M 《Biotechnology journal》2006,1(7-8):787-794
It needs three factors to build an industry: market demand, product vision and capital. White biotechnology already produces high volume products such as feed additive amino acids and specialty products like enzymes for enantioselective biocatalysis. It serves large and diverse markets in the nutrition, wellness, pharmaceutical, agricultural and chemical industry. The total volume adds up to $ 50 billion worldwide. In spite of its proven track record, white biotechnology so far did not attract as much capital as red and even green biotechnology. However, the latest finance indicators confirm the continuously growing attractiveness of investment opportunities in white biotechnology. This article discusses white biotechnology's position and potential in the finance market and success factors. 相似文献
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工业生物催化是继医药、农业之后的生物技术第三次浪潮。从21世纪化学工业发展的前沿特点,介绍生物催化加工过程及生产方式,主要解决传统产业改造和新的应用领域的开拓,提出发展生物催化产业的策略和加强支持力度的建设。 相似文献
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Nowadays, there is an increasing demand to establish new feasible, efficient downstream processing (DSP) techniques in biotechnology and related fields. Although several conventional DSP technologies have been widely employed, they are usually expensive and time-consuming and often provide only low recovery yields. Hence, the DSP is one major bottleneck for the commercialization of biological products. In this context, polyethylene glycol (PEG)–salt aqueous two-phase systems (ATPS) represent a promising, efficient liquid–liquid extraction technology for the DSP of various biomolecules, such as proteins and enzymes. Furthermore, ATPS can overcome the limitations of traditional DSP techniques and have gained importance for applications in several fields of biotechnology due to versatile advantages over conventional DSP methods, such as biocompatibility, technical simplicity, and easy scale-up potential. In the present review, various practical applications of PEG–salt ATPS are presented to highlight their feasibility to operate as an attractive and versatile liquid–liquid extraction technology for the DSP of proteins and enzymes, thus facilitating the approach of new researchers to this technique. Thereby, single- and multi-stage extraction, several process integration methods, as well as large-scale extraction and purification of proteins regarding technical aspects, scale-up, recycling of process chemicals, and economic aspects are discussed. 相似文献
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Bo Mattiasson 《Trends in biotechnology》1983,1(1):16-20
Aqueous two-phase systems can be employed in several areas of biotechnology including the purification of biomolecules, cells and organelles and increasing the speed of product-inhibited fermentations such as the production of acetone, butanol and ethanol. Furthermore, separations in aqueous two-phase systems have been successfully applied in binding assays. 相似文献
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The PEG-Ficoll polymer phase system is one that has been overlooked in the past for biotechnology applications because of the stability of its emulsions. However, new applications, such as emulsion coating of cells, are appearing that rely on this very property. Ficoll is highly polydisperse and multimodal with three distinct Ficoll peaks in gel permeation chromatography. As a result, the transition between one-phase and two-phase systems is blurred and the binodials obtained through turbidometric titration and tie-line analysis differ significantly. Moreover, since the three Ficoll peaks partition differently, tie-line analysis cannot be described by a simple model of the aqueous two-phase system. A simple modification to the model allowed for excellent fit, and this modification may prove well-suited for the many practical cases where aqueous two-phase systems fail to display parallel tie-lines as implicitly assumed in the simpler model. 相似文献
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An extractive biocatalytic method of aqueous two-phase system was employed for stereoinversing (R)-1-phenyl-1,2-ethanediol into (S)-1-phenyl-1,2-ethanediol by Candida parapsilosis CCTCC M203011. It was observed that substrate and product inhibitions in microbial stereoinversion through one-pot oxidation
and reduction were removed efficiently by extractive biocatalysis in aqueous two-phase system with PEG 4000/phosphate potassium
system, and that the substrate concentration was enhanced from 15 to 30 g/L with product optical purity of 99.02% e.e. and
yield of 90% after 60 h. Simultaneously, it was observed that change in cell morphology impedes the further enhancement of
substrate concentration in this system but can be reversibly changed after stereoinversion or cultivation in systems without
PEG. 相似文献
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The different kinds of aqueous two-phase systems for accepted or potential use in biotechnology are summarized. Some properties of interest for the extractive use are discussed. 相似文献
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The development of biotechnological processes using novel two-phase systems based on molten salts known as ionic liquids (ILs)
got into the focus of interest. Many new approaches for the beneficial application of the interesting solvent have been published
over the last years. ILs bring beneficial properties compared to organic solvents like nonflammability and nonvolatility.
There are two possible ways to use the ILs: first, the hydrophobic ones as a substitute for organic solvents in pure two-phase
systems with water and second, the hydrophilic ones in aqueous two-phase systems (ATPS). To effectively utilise IL-based two-phase
systems or IL-based ATPS in biotechnology, extensive experimental work is required to gain the optimal system parameters to
ensure selective extraction of the product of interest. This review will focus on the most actual findings dealing with the
basic driving forces for the target extraction in IL-based ATPS as well as presenting some selected examples for the beneficial
application of ILs as a substitute for organic solvents. Besides the research focusing on IL-based two-phase systems, the
“green aspect” of ILs, due to their negligible vapour pressure, is widely discussed. We will present the newest results concerning
ecotoxicity of ILs to get an overview of the state of the art concerning ILs and their utilisation in novel two-phase systems
in biotechnology. 相似文献