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1.
Female carriers of the T(5;12)31H reciprocal translocation had an average reduction of 73% in oocyte numbers compared with normal litter mates, which was of a magnitude similar to the reduction in sperm counts of male carriers. Analysis of synaptonemal complexes showed that the translocated chromosomes appeared as quadrivalents, or trivalents and univalents, or bivalents in both sexes. Quadrivalents were of three types: fully synapsed, with asynapsis confined to breakpoints, and with unsynapsed ends. There was more pairing in spermatocytes than in oocytes: 37% of spermatocytes, but only 14% of oocytes, contained a fully synapsed quadrivalent, and trivalents were also more frequently fully synapsed in spermatocytes. When these results are compared with those previously obtained for other chromosome anomalies, it becomes evident that there are considerable differences in chromosome pairing between males and females, and that different chromosome rearrangements differ in the relative amount of pairing failure occurring in male and female carriers.  相似文献   

2.
Pairing failure at meiosis has been postulated as a cause of gametogenic arrest in both heterozygous translocation carriers and males whose spermatocytes exhibit univalent X and Y chromosomes. The present investigation is a survey of pachytene translocation configurations, at the electron microscopic level, in six stocks of mice, comprising a total of 464 spermatocytes and 343 oocytes. Univalence of the X and Y chromosomes was studied in the same stocks, as well as in three additional homozygous translocation stocks. Fully paired as well as asynaptic configurations were found in all translocation stocks, and the proportions of each configuration differed considerably between spermatocytes and oocytes of mice carrying the same translocation. In both spermatocytes and oocytes, other pairing anomalies were more frequent in cells with asynapsed than with fully synapsed configurations, and spermatocytes with univalent sex chromosomes had a higher proportion of autosomal anomalies than did spermatocytes with XY bivalents. It is concluded that pairing failure at meiosis is primarily a symptom, rather than a cause, of gametogenic arrest, and that chromosome rearrangements, even if they appear to be balanced, may affect the rate of atresia by interfering with the normal rate of meiotic progression. Once pairing failure is established, it could secondarily increase the probability of gametogenic failure.  相似文献   

3.
Reduced oocyte numbers in tertiary trisomic mice with male sterility   总被引:1,自引:0,他引:1  
Oocyte counts carried out in 3- to 5-day-old tertiary trisomic Ts(5(12))31H mice revealed a mean reduction of 71% in the number of oocytes as compared with that of normal littermates. The pool of small oocytes was reduced by 75%, and the number of growing oocytes by 8%. The sperm count of the trisomic males was less than 1% of normal, with most spermatozoa being abnormal (Beechey et al., 1980). These results indicate that the presence of the extra 5(12) chromosome, which causes male sterility, also has a marked effect on oogenesis. Possible reasons for the difference in severity of the gametogenic impairment in males and females are discussed.  相似文献   

4.
To correlate the chromosomal constitution of meiotic cells with possible disturbances in spindle function and the etiology of nondisjunction, we examined the spindle apparatus and chromosome behavior in maturing oocytes and analyzed the chromosomal constitution of metaphase II-arrested oocytes of CD/Cremona mice, which are heterozygous for a large number of Robertsonian translocation chromosomes (18 heterobrachial metacentrics in addition to two acrocentric chromosomes 19 and two X chromosomes). Spreading of oocytes during prometaphase 1 revealed that nearly all oocytes of the heterozygotes contained one large ring multivalent, apart from the bivalents of the two acrocentric chromosomes 19 and the X chromosomes, indicating that proper pairing and crossing-over between the homologous chromosome arms of all heterobrachial chromosomes took place during prophase. A large proportion of in vitro-matured oocytes arrested in metaphase II exhibited numerical chromosome aberrations (26.5% hyperploids, 40.8% hypoploids, and 6.1% diploids). In addition, some of the oocytes with euploid chromosome numbers (26.5% of the total examined) appeared to be nullisomic for one chromosome and disomic for another chromosome, so that aneuploidy levels may even be higher than expected on the basis of chromosome counts alone. Although oocytes of the complex heterozygous mice seemed able initially to form a bipolar spindle during first prometaphase, metaphase I spindles were frequently asymmetrical. Chromosomes in the multivalent did not align properly at the equator, centromeres of neighboring chromosomes in the multivalent remained maloriented, and pronounced lagging of chromosomes was observed at telophase I in oocytes obtained from the Robertsonian translocation heterozygotes. Therefore, disturbance in spindle structure and chromosome behavior appear to correlate with the chromosomal constitution in these oocytes and, ultimately, with failures in proper chromosome separation. In particular, reorientation appears to be a rare event, and malorientation of chromosomes may remain uncorrected throughout prometaphase, as we could not find many typical metaphase I stages in heterozygotes. This, in turn, could be the basis for malsegregation at anaphase and may ultimately induce a high rate of nondisjunction and aneuploidy in the oocytes of CD/Cremona mice, leading to total sterility in heterozygous females.  相似文献   

5.
Abnormal patterns of meiotic recombination (i.e., crossing-over) are believed to increase the risk of chromosome nondisjunction in human oocytes. To date, information on recombination has been obtained using indirect, genetic methods. Here we use an immunocytological approach, based on detection of foci of a DNA mismatch-repair protein, MLH1, on synaptonemal complexes at prophase I of meiosis, to provide the first direct estimate of the frequency of meiotic recombination in human oocytes. At pachytene, the stage of maximum homologous chromosome pairing, we found a mean of 70.3 foci (i.e., crossovers) per oocyte, with considerable intercell variability (range 48-102 foci). This mean equates to a genetic-map length of 3,515 cM. The numbers and positions of foci were determined for chromosomes 21, 18, 13, and X. These chromosomes yielded means of 1.23 foci (61.5 cM), 2.36 foci (118 cM), 2.5 foci (125 cM), and 3.22 foci (161 cM), respectively. The foci were almost invariably located interstitially and were only occasionally located close to chromosome ends. These data confirm the large difference, in recombination frequency, between human oocytes and spermatocytes and demonstrate a clear intersex variation in distribution of crossovers. In a few cells, chromosomes 21 and 18 did not have any foci (i.e., were presumptively noncrossover); however, configurations that lacked foci were not observed for chromosomes 13 and X. For the latter two chromosome pairs, the only instances of absence of foci were observed in abnormal cells that showed chromosome-pairing errors affecting these chromosomes. We speculate that these abnormal fetal oocytes may be the source of the nonrecombinant chromosomes 13 and X suggested, by genetic studies, to be associated with maternally derived chromosome nondisjunction.  相似文献   

6.
Univalent sex chromosomes in spermatocytes of Sxr-carrying mice   总被引:1,自引:0,他引:1  
Pachytene configurations of the sex chromosomes were studied in whole-mount, silver-stained preparations of spermatocytes in mice with XY,Sxr, XX,Sxr, XO,Sxr, XO,Sxr+512 and T(X;4)37H,YSxr chromosomes, and non-Sxr-carrying controls. XY,Sxr males showed an increased number of X and Y univalents and of self-synapsed Y chromosomes. In T(X;4)37H,YSxr males an increased proportion of trivalent+Y configurations was also accompanied by higher numbers of self-paired Y univalents; the proportion of trivalent+X4 was not increased, but that of self-synapsed X4 univalents was. There was more selfsynapsis in cells containing one univalent than in cells containing two univalents. Spermatocytes of XX,Sxr mice contained single univalent X, which was never seen to be self-synapsed, but self-synapsis of the X occurred in a proportion of cells in XO,Sxr males. There were no self-paired X chromosomes in the XO,Sxr+512 mouse although lowlevel pairing of the 512 chromosome occurred. All four XX,Sxr and XO,Sxr males contained testicular sperm, and testicular sperm were also present in one T(X;4)37H male, while another such male had sperm in the caput. It is concluded that (1) self-synapsis of univalents is affected by variable conditions in the cell as well as by the DNA sequences of the chromosome, and (2) that the level of achievable spermatogenesis is not always rigidly predetermined by a chromosome anomaly but can be modulated by the genetic background.  相似文献   

7.
Pairing of pachytene chromosomes was studied in oocytes and spermatocytes of mice heterozygous for the male-sterile Is(7;1)40H insertion using light and electron microscopy for synaptonemal complex analysis in surface-spread, silver-stained preparations. The data comprised four males and four female embryos. The insertion/deletion configurations appeared as either two bivalents or one quadrivalent in both sexes, but the proportion of bivalents was higher in oocytes. Some insertion and deletion bivalents showed synaptic adjustment. The insertion/deletion configurations were associated with, or adjacent to, the XY bivalent in the majority of spermatocytes. End-to-end association of different bivalents was more frequent in oocytes than in spermatocytes. It is suggested that physiological differences between male and female gametocytes may lead to the difference in their reproductive potential.The authors warmly dedicate this paper to the Founder and Senior Editor of Chromosoma, Professor Hans Bauer, on the occasion of his 80th birthday.  相似文献   

8.
The prophase oocytes of two murine Robertsonian translocation (Rb) trisomies of chromosomes 16 and 19 were investigated using electron microscopy and a whole-cell micro-spreading technique after silver staining. About 20% of fetuses of each type were trisomic. They were obtained by mating animals heterozygous for two Rb's, monobrachially homologous for either chromosome 16 or 19, to an entirely acrocentric stock. Because of the almost inevitable prenatal mortality of the trisomic embryos, their fetal ovaries were "rescued" by an in vitro method for prophase studies. Analysis of the recovered oocytes showed frequent, close pairing associations of the three trisomic axes and evidence suggesting that the closely apposed axes coincided with the side-by-side formation of parallel, complete, true synaptonemal complexes; hence, the cytogenetic dogma that pairing is always two-by-two was contradicted. The presence of two parallel complexes has implications for crossing-over recombination. Triple associations of axes were found in almost half the trisomy 19 (Ts19) and in about 70% of the trisomy 16 (Ts16) prophases. The extent of triple associations varied and was greater in Ts16 than in Ts19 oocytes. Other relevant observations concerned the proportions of univalents and of univalence of the trisomic axes (21% in Ts16 and 46% in Ts19) and the distinctive, thickened appearance of all univalent axes. The pairing behaviour observed in balanced heterozygotes confirms what appears to be nonhomologous pairing and synaptic adjustment within the short-arm axes of the Rb trivalents.  相似文献   

9.
家鸡联会复合体的亚显微结构分析   总被引:1,自引:0,他引:1  
刘冬梅  张传善 《动物学报》1990,36(4):360-365
本文以表面铺展——硝酸银染色技术,对家鸡的联会复合体(Syneptonemal Complex,SC)作亚显微结构分析。根据对10个精母细胞和10个卵母细胞SC的测量结果,绘制组型图。发现雌雄家鸡的常染色体的SC组型相同。在精母细胞中,性染色体(ZZ)的行为与常染色体相似。在卵母细胞中,性染色体ZW的长度不同,长轴为Z,短轴为W,两者之间只有部分配对,形成SC。从早粗线期到晚粗线期,由同源配对调整为非同源配对。另外,在一只雌鸡中,第一次观察到,有些细胞的常染色体能正常配对,而性染色体完全不配对的现象。  相似文献   

10.
Surface-spread, silver-stained primary spermatocytes from individuals of the Sitka deer mouse (Peromyscus sitkensis) were analyzed by electron microscopy. Pairing of the X and Y chromosomes is initiated at early pachynema and is complete by mid pachynema. The pattern of sex chromosome pairing is unique in that it is initiated at an interstitial position, with subsequent synapsis proceeding in a unidirectional fashion towards the telomeres of the homologous segments. One-third the length of the X and two-thirds the length of the Y are involved in the synaptonemal complex of the sex bivalent. Various morphological complexities develop in the heteropycnotic (unpaired) segments as pachynema progresses, but desynapsis is not initiated until diplonema. Analysis of C-banded diakinetic nuclei indicated that sex chromosome pairing involves the heterochromatic short arm of the X and the long arm of the heterochromatic Y. An interstitial chiasma between the X and Y was observed in the majority of the diakinetic nuclei. The observation of a substantial pairing region and chiasma formation between the sex chromosomes of these deer mice is interpreted as indicating homology between the short arm of the X and the long arm of the Y.  相似文献   

11.
Using fluorescence in situ hybridization with human band-specific DNA probes we examined the effect of ionizing radiation on the intra-nuclear localization of the heterochromatic region 9q12-->q13 and the euchromatic region 8p11.2 of similar sized chromosomes 9 and 8 respectively in confluent (G1) primary human fibroblasts. Microscopic analysis of the interphase nuclei revealed colocalization of the homologous heterochromatic regions from chromosome 9 in a proportion of cells directly after exposure to 4 Gy X-rays. The percentage of cells with paired chromosomes 9 gradually decreased to control levels during a period of one hour. No significant changes in localization were observed for chromosome 8. Using 2-D image analysis, radial and inter-homologue distances were measured for both chromosome bands. In unexposed cells, a random distribution of the chromosomes over the interphase nucleus was found. Directly after irradiation, the average inter-homologue distance decreased for chromosome 9 without alterations in radial distribution. The percentage of cells with inter-homologue distance <3 micro m increased from 11% in control cells to 25% in irradiated cells. In contrast, irradiation did not result in significant changes in the inter-homologue distance for chromosome 8. Colocalization of the heterochromatic regions of homologous chromosomes 9 was not observed in cells irradiated on ice. This observation, together with the time dependency of the colocalization, suggests an underlying active cellular process. The biological relevance of the observed homologous pairing remains unclear. It might be related to a homology dependent repair process of ionizing radiation induced DNA damage that is specific for heterochromatin. However, also other more general cellular responses to radiation-induced stress or change in chromatin organization might be responsible for the observed pairing of heterochromatic regions.  相似文献   

12.
Bertini V  Viola D  Vitti P  Simi P  Valetto A 《Gene》2012,503(1):123-125
We report on a 36-year-old infertile woman, presenting a premature ovarian failure with an otherwise normal female phenotype. Cytogenetic analyses showed the presence of a supernumerary marker chromosome, that was characterized by FISH (fluorescent in situ hybridization) and array CGH (comparative genomic hybridization). This marker chromosome was derived from chromosome 15, and contained only heterochromatic material. The Prader Willi/Angelman region was not present. No duplications of the 15q regions were detected by array CGH. Supernumerary markers of chromosome 15 have been reported in cases of infertility and amenorrhea, that is also described in cases with marker derived by other acrocentric chromosomes. The case here presented constitutes a further example that etiology of POF is not always associated with a defective gene, but in some cases oocytes atresia can be the consequence of the abnormal meiotic pairing of chromosomes.  相似文献   

13.
H Wallace  B M Wallace 《Génome》1995,38(6):1105-1111
The longest chromosome (number 1) of Trituturus cristatus carries a heteromorphic segment, a heterozygosity perpetuated by a balanced lethal system. The heteromorphic segment is regarded as achiasmate and has been claimed to be asynaptic. Direct observations of chromosome pairing in spermatocytes and oocytes yield some cases where all homologous chromosomes appear to be completely paired, but the individual bivalents could not be identified as pachytene is not particularly clear in this species. The long arms of bivalent 1 usually remain attached by a terminal chiasma in spermatocytes of T. c. cristatus but the corresponding chiasma is only rarely present in T. c. carnifex spermatocytes. Synaptonemal complexes have been measured in both spermatocytes and oocytes of T. c. cristatus. A karyotype constructed from these measurements matches the main features of somatic and lampbrush chromosome karyotypes, indicating that all chromosomes must be completely paired and proportionately represented as synaptonemal complex. The total length of synaptonemal complex is much the same in spermatocytes and oocytes and is similar to the length in spermatocytes of Xenopus laevis. These two amphibian examples supplement a recent survey of other vertebrate classes to reinforce its conclusion that synaptonemal complex length is not related to genome size in vertebrates.  相似文献   

14.
Pachytene nuclei were studied in five tertiary trisomic male mice of Ts(113)70H and two of Ts(512)31H, with special attention given to the sex vesicles. Silver stained air dried cells analysed by light microscopy were used mainly, but in addition one sample of surface spread, ethanolic phosphotungstic acid stained nuclei was analysed by electron microscopy. With both techniques and both karyotypes, the extra chromosome (or the greater part of it) almost consistently aggregated with the sex chromosomes. Thereby, the chromatin structure of the extra chromosome as judged by a fine granular appearance resembled that of the sex chromosomes. The animals used ranged from almost azospermic to fertile oligospermic. This variation was not reflected in the position and morphology of the chromosomes 113 and 512. — Using the whole mount spreading EM technique within a Ts(113)70H tertiary trisomic, both 13;13;113 trivalents and 113 univalents were observed. The 13;13;113 trivalents showed a variety of morphologies, ranging from a situation showing classical partner exchange to complete synapsis between the two 13 homologues with the 113 telomeric region adhering. The latter configuration is thought not to lead to chiasma formation.  相似文献   

15.
The pairing behavior of the sex chromosomes in male and female individuals representing seven species of Peromyscus was analyzed by electron microscopy of silver-stained zygotene and pachytene configurations. Six species possess submetacentric or metacentric X chromosomes with heterochromatic short arms. Sex-chromosome pairing in these species is initiated during early pachynema at an interstitial position on the X and Y axes. Homologous synapsis then progresses in a unidirectional fashion towards the telomeres of the X short arm and the corresponding arm of the heterochromatic Y chromosome. The distinctive pattern of synaptic initiation allowed a late-synapsing bivalent in fetal oocytes to be tentatively identified as that of the X chromosomes. In contrast to the other species, Peromyscus megalops possesses an acrocentric X chromosome and a very small Y chromosome. Sex-chromosome pairing in this species is initiated at the proximal telomeric region during late zygonema, and then proceeds interstitially towards the distal end of the Y chromosome. These observations suggest that the presence of X short-arm heterochromatin and corresponding Y heterochromatin interferes with late-zygotene alignment of the pairing initiation sites, thereby delaying XY synaptic initiation until early pachynema. The pairing initiation sites are conserved in the vicinity of the X and Y centromeres in Peromyscus, and consequently the addition of heterochromatin during sex-chromosome evolution essentially displaces these sites to an interstitial position.  相似文献   

16.
F Marec  W Traut 《Génome》1994,37(3):426-435
Structure and pairing behavior of sex chromosomes in females of four T(W;Z) lines of the Mediterranean flour moth, Ephestia kuehniella, were investigated using light and electron microscopic techniques and compared with the wild type. In light microscopic preparations of pachytene oocytes of wild-type females, the WZ bivalent stands out by its heterochromatic W chromosome strand. In T(W;Z) females, the part of the Z chromosome that was translated onto the W chromosome was demonstrated as a distal segment of the neo-W chromosome, displaying a characteristic non-W chromosomal chromomere-interchromomere pattern. This segment is homologously paired with the corresponding part of a complete Z chromosome. In contrast with the single ball of heterochromatic W chromatin in highly polyploid somatic nuclei of wild-type females, the translocation causes the formation of deformed or fragmented W chromatin bodies, probably owing to opposing tendencies of the Z and W chromosomal parts of the neo-W. In electron microscopic preparations of microspread nuclei, sex chromosome bivalents were identified by the remnants of electron-dense heterochromatin tangles decorating the W chromosome axis, by the different lengths of the Z and W chromosome axes, and by incomplete pairing. No heterochromatin tangles were attached to the translocated segment of the Z chromosome at one end of the neo-W chromosome. Because of the homologous pairing between the translocation and the structurally normal Z chromosome, pairing affinity of sex chromosomes in T(W;Z) females is significantly improved. Specific differences observed among T(W;Z)1-4 translocations are probably due to the different lengths of the translocated segments.  相似文献   

17.
The facultative heterochromatic X chromosome in leptotene spermatocytes of the grasshopper Eyprepocnemis plorans showed marked hypoacetylation for lysine 9 in the H3 histone (H3-K9) with no sign of histone H2AX phosphorylation. Since H3-K9 hypoacetylation precedes the meiotic appearance of phosphorylated H2AX (gamma-H2AX), which marks the beginning of recombinational DNA double-strand breaks (DSBs), it seems that meiotic sex-chromosome inactivation (MSCI) in this grasshopper occurs prior to the beginning of recombination and hence synapsis (which in this species begins later than recombination). In addition, all constitutively heterochromatic chromosome regions harbouring a 180-bp tandem-repeat DNA and rDNA (B chromosomes and pericentromeric regions of A chromosomes) were H3-K9 hypoacetylated at early leptotene even though they will synapse at subsequent stages. This also suggests that meiotic silencing in this grasshopper might be independent of synapsis. The H3-K9 hypoacetylated state of facultative and constitutive heterochromatin persisted during subsequent meiotic stages and was even apparent in round spermatids. Finally, the fact that B chromosomes are differentially hypoacetylated in testis and embryo interphase cells suggests that they might be silenced early in development and remain this way for most (or all) life-cycle stages.  相似文献   

18.
Chiasma frequency and position were analyzed at a predominantly late diplotene-diakinesis stage of the first meiotic division in oocytes and spermatocytes from T(1;13)70H homozygotes and heterozygotes, T(2;8)26H heterozygotes and from Ts(I13)70H tertiary trisomics of the mouse, Mus musculus. For T70H/T70H, the 131 long marker bivalent was studied and for the other karyotypes, analysis was confined to the multivalent configurations adopted by the rearranged chromosomes and their homologues. For the 131 bivalent, the chiasma frequency tended to be increased in the female. For the T26H and the T70H multivalents, the chiasma frequencies were higher in the female, largely due to the much higher values in the short interstitial segments. This observed enhancement has been attributed to pairing differences rather than to differences in chiasma forming capability. Both in the telomeric region of the 131 bivalent and in the short translocated segments of the reciprocal translocation and tertiary trisomic multivalents, females showed fewer chiasmata than males. The determinations of chiasma position in the 131 bivalent indicated chiasma interference with respect to position, leading to clustering of chiasmata somewhat beyond the centromere and towards the telomere of this chromosome.  相似文献   

19.
We investigated the behaviour of centromeres and distal telomeres during the initial phases of female meiosis in mice. In particular, we wished to determine whether clustering of centromeres and telomeres (bouquet formation) played the same crucial role in homologous chromosome pairing in female meiosis as it does in the male. We found that synapsis (intimate homologous chromosome pairing) is most frequently initiated in the interstitial regions of homologous chromosomes, apparently ahead of the distal regions. The proximal ends of the chromosomes appear to be disfavoured for synaptic initiation. Moreover, initiation of synapsis occurred in oocytes that showed little or no evidence of bouquet formation. A bouquet was present in a substantial proportion of cells at mid to late zygotene, and was still present in some pachytene oocytes. This pattern of bouquet formation and pairing initiation is in stark contrast to that previously described in the male mouse. We propose that although dynamic movements of centromeres and telomeres to form clusters may facilitate alignment of homologues or homologous chromosome segments during zygotene, in the female mouse positional control of synaptic initiation is dependent on some other mechanism.  相似文献   

20.
The diploid chromosome number of the Chinese raccoon dog varies from 54 (no B chromosomes) to 58 (4 B chromosomes). The B chromosomes are totally heterochromatic. An electron microscopic study was made of the synaptonemal complexes (SC) in spermatocytes of these animals. The SC karyotype consists of 27 regular chromosome pairs (autosomes and the sex chromosomes) plus the B chromosomes. The Bs pair effectively with one another at pachytene, but the SC axes of the B chromosomes are much denser than those of the A chromosomes. Depending on the number of Bs, both bivalents and multivalents have been observed. When three B chromosomes are present in a cell, parallel alignment of all three SCs can be seen. Formation of multivalents indicates high homology among these supernumerary heterochromatic chromosomes. Fusiform bulges are found along unpaired regions of all chromosomes which are particularly pronounced in diplotene.  相似文献   

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