A modified drinking water quality index (DWQI) for assessing drinking source water quality in rural communities of Khuzestan Province,Iran

We reconsidered the Canadian Council of Ministers of the Environment Water Quality Index (CCME WQI) to achieve an efficient drinking water quality index (DWQI) for assessment of drinking source water quality in rural communities of Khuzestan Province, Iran in 2009–2013. In contribution with a panel of water quality experts, the CCME WQI was mainly modified by four changes: (1 and 2) assigning weight factors for input parameters and index factors, (3) modifying excursion concept for carcinogens and bioaccumulative pollutants and (4) removing effect of unequal measurements of input parameters. The DWQI characterizes the drinking source water quality through comparing the measured values of input parameters with relevant benchmarks. The DWQI score (from 0 to 100) classifies the water quality in five categories as poor (0–54.9), marginal (55.0–69.9), fair (70.0–84.9), good (85.0–94.9) and excellent (95.0–100). Based on the DWQI, the temporal changes of the rural drinking source water quality were not significant; while the spatial variations of the water quality were considerable across the province, so the DWQI scores in the northern counties were higher than that in the southern ones. At the county level, the highest and lowest average scores of the DWQI (±standard deviation: SD) were observed in Izeh and Shadegan to be 90 ± 5 and 69 ± 10, respectively. Based on the DWQI, proportions of the drinking water sources with the excellent, good, fair, marginal and poor qualities were determined to be 6.7, 59.1, 26.2, 7.8 and 0.1%, respectively. Turbidity and Ryznar Index (RI) were introduced respectively as the health-based and esthetic parameters with the most violations (22.7 and 63.2%, respectively). The results of the case study and sensitivity analysis indicated that the DWQI is a simple, flexible, stable and reliable index and could be used as an effective tool to characterize drinking source water quality.     

Biostratigraphy and paleoecology of the Oligo-Miocene succession in Fars and Khuzestan areas (Zagros Basin,SW Iran)

Paleontological and biostratigraphical studies on carbonate platform succession from southwest Iran documented a great diversity of shallow-water benthic foraminifera during the Oligocene–Miocene. Larger foraminifera are the main means for the stratigraphic zonation of carbonate sediments. The distributions of larger benthic foraminifera in two outcrop sections (Abolhayat and Lali) in the Zagros Basin, Iran, are used to determine the age of the Asmari Formation. Four assemblage zones have been recognized by distribution of the larger benthic foraminifera in the study areas. Assemblage 3 (Aquitanian age) and 4 (Burdigalian age) have not been recognized in the Abolhayat section (Fars area), due to sea-level fall. The end Chattian sea-level fall restricted marine deposition in the Abolhayat section and Asmari Formation replaced laterally by the Gachsaran Formation. This suggests that the Miocene part of the formation as recognized in the Lali section (Khuzestan area) of the Zagros foreland basin is not present in the Abolhayat outcrop. The distribution of the Oligocene larger benthic foraminifera indicates that shallow marine carbonate sediments of the Asmari Formation at the study areas have been deposited in the photic zone of tropical to subtropical oceans. Based on analysis of larger benthic foraminiferal assemblages and microfacies features, three major depositional environments are identified. These include inner shelf, middle shelf and outer shelf. The inner shelf facies is characterized by wackestone–packstone, dominated by various taxa of imperforate foraminifera. The middle shelf is represented by packstone–grainstone to floatstone with a diverse assemblage of larger foraminifera with perforate wall. Basinwards is dominated by argillaceous wackestone characterized by planktonic foraminifera and large and flat nummulitidae and lepidocyclinidae. Planktonic foraminifera wackestone is the dominant facies in the outer shelf.     

Application of diagnostic methods and molecular diagnosis of hemoglobin disorders in Khuzestan province of Iran

BACKGROUND:

The hemoglobinopathies refer to a diverse group of inherited disorders characterized by a reduced synthesis of one or more globin chains (thalassemias) or the synthesis of structurally abnormal hemoglobin (Hb). The thalassemias often coexist with a variety of structural Hb variants giving rise to complex genotypes and an extremely wide spectrum of clinical and hematological phenotypes. Hematological and biochemical investigations and family studies provide essential clues to the different interactions and are fundamental to DNA diagnostics of the Hb disorders. Although DNA diagnostics have made a major impact on our understanding and detection of the hemoglobinopathies, DNA mutation testing should never be considered a shortcut or the test of first choice in the workup of a hemoglobinopathy.

MATERIALS AND METHODS:

A careful three-tier approach involving: (1) Full blood count (2) Special hematological tests, followed by (3) DNA mutation analysis, provides the most effective way in which to detect primary gene mutations as well as gene-gene interactions that can influence the overall phenotype. With the exception of a few rare deletions and rearrangements, the molecular lesions causing hemoglobinopathies are all identifiable by PCR-based techniques. Furthermore, each at-risk ethnic group has its own combination of common Hb variants and thalassemia mutations. In Iran, there are many different forms of α and β thalassemia. Increasingly, different Hb variants are being detected and their effects per se or in combination with the thalassemias, provide additional diagnostic challenges.

RESULTS:

We did step-by-step diagnosis workup in 800 patients with hemoglobinopathies who referred to Research center of Thalassemia and Hemoglobinopathies in Shafa Hospital of Ahwaz Joundishapour University of medical sciences, respectively. We detected 173 patients as iron deficiency anemia (IDA) and 627 individuals as thalassemic patients by use of different indices. We have successfully detected 75% (472/627) of the β-thalassemia mutations by using amplification refractory mutation system (ARMS) technique and 19% (130/627) of the β-thalassemia mutations by using Gap-PCR technique and 6% (25/627) as Hb variants by Hb electrophoresis technique. We did prenatal diagnosis (PND) for 176 couples which had background of thalassemia in first pregnancy. Result of PND diagnosis in the first trimester was 35% (62/176) affected fetus with β-thalassemia major and sickle cell disease that led to termination of the pregnancy.

CONCLUSION:

Almost all hemoglobinopathies can be detected with the current PCR-based assays with the exception of a few rare deletions. However, the molecular diagnostic service is still under development to try and meet the demands of the population it serves. In the short term, the current generation of instruments such as the capillary electrophoresis systems, has greatly simplified DNA sequence analysis.     

Lung compliance, plasma electrolyte levels and acid-base balance are affected by scorpion envenomation in anesthetized rats under mechanical ventilation

To determine the effects of Tityus serrulatus scorpion toxin on lung compliance and resistance, ionic equilibrium and acid-base balance over time in anesthetized and mechanically ventilated rats, we measured air flow, tracheal and esophageal pressure. Lung volume was obtained by electronic integration of airflow signal. Arterial blood samples were collected through a catheter at baseline (before) and 5, 15, 30 and 60 min after scorpion toxin injection for arterial blood gases, bicarbonate, and alkali reserve levels as well as for, sodium, potassium, magnesium, glucose, lactate, hematocrit, and osmolality analysis. Injection of the gamma fraction of the T. serrulatus scorpion venom in rats under mechanical ventilatory support leads to a continuous decrease in lung compliance secondary to pulmonary edema, but no change in airway resistance. The changes in arterial blood gases characterizing metabolic acidosis were accompanied by an increase in arterial lactate and glucose values, suggesting a scorpion toxin-induced lactic acidosis, in association with poor tissue perfusion (hypotension and low cardiac output). Moreover, scorpion toxin injection resulted in hyperosmolality, hyperkalemia, hypermagnesemia and an increase in hematocrit. The experiments have shown a clinically relevant animal model to study severe scorpion envenoming and may help to better understand the scorpion envenoming syndrome.     

Breastfeeding knowledge,attitude and practice among school teachers in Abha female educational district,southwestern Saudi Arabia

ABSTRACT: BACKGROUND: Inadequate knowledge, or inappropriate practice, of breastfeeding may lead to undesirable consequences. The aim of this study was to assess breastfeeding knowledge, attitude and practice (KAP) among female teachers in the Abha Female Educational District and identify factors that may affect breastfeeding practice in the study population. METHODS: A cross-sectional study using a self-administered questionnaire was conducted among school teachers in Abha Female Educational District during the months of April to June, 2011. Breastfeeding KAP of participants who had at least one child aged five years or younger at the time of the study were assessed using a self-administered questionnaire, based on their experience with the last child. RESULTS: A total of 384 women made up of 246 (61.1%) primary-, 89 (23.2%) intermediate- and 49 (12.8%) high-school teachers participated in the study. One hundred and nineteen participants (31%) started breastfeeding their children within one hour of delivery, while exclusive breastfeeding for 6 months was reported only by 32 (8.3%) participants. Insufficient breast milk and work related problems were the main reasons given by 169 (44%) and 148 (38.5%) of participants, respectively, for stopping breastfeeding before two years. Only 33 participants (8.6%) had attended classes related to breastfeeding. However, 261 participants (68%) indicated the willingness to attend such classes, if available, in future pregnancies. CONCLUSIONS: This study revealed that breast milk insufficiency and adverse work related issues were the main reasons for a very low rate of exclusive breastfeeding among female school teachers in Abha female educational district, Saudi Arabia. A very low rate of attending classes addressing the breastfeeding issues during pregnancy, and an alarming finding of a high percentage of babies receiving readymade liquid formula while still in hospital, were also brought out by the present study. Such findings, if addressed comprehensively by health care providers and decision-makers, will lead to the improvement of breastfeeding practices in the study community.     

Massive acute ischemic stroke after Bothrops spp. envenomation in southwestern Colombia: Case report and literature review

Bothrops spp. envenomation and its relationship with ischemic stroke has complex pathogenesis. Local effects such as edema, pain, redness, necrosis, and systemic manifestations like coagulation disorders, thrombosis, renal failure, and hemorrhage have been reported. Hemorrhagic stroke is a common neurological complication but ischemic stroke is poorly understood.We present here the case of a 50-year-old male with no comorbidities referred from a rural area in southwest Colombia with a Bothrops spp. snakebite on the left hand. On admission, the patient presented with a deterioration of consciousness and required mechanical ventilation assistance. The MRI showed multiple ischemic areas in the bilateral frontal- temporal and occipital regions. Two months later, the patient had a favorable resolution, although central paresis in the III and VI cranial nerves and positive Babinski’s sign persisted. As already mentioned, the pathophysiology of ischemic stroke due to snakebite is complex but the procoagulant activity of the venom components, the hypovolemic shock, the endothelial damage, and the thromboinflammation can explain it, and although it rarely occurs, it should be considered as a complication of ophidian accidents caused by Bothrops spp.     

Structure and regeneration of canopy species in an old-growth evergreen broad-leaved forest in Aya district,southwestern Japan

The population structure and regeneration of canopy species were studied in a 4 ha plot in an old-growth evergreen broad-leaved forest in the Aya district of southwestern Japan. The 200 m × 200 m plot contained 50 tree species, including 22 canopy species, 3,904 trees (dbh5 cm) and a total basal area of 48.3 m²/ha. Forty one gaps occurred within the plot, and both the average gap size (67.3 m²) and the total area of gap to plot area (6.9%) were small. Species found in the canopy in the plot were divided into three groups (A, B, C) based on size and spatial distribution patterns, and density in each tree size. Group A (typical species: Distylium racemosum, Persea japonica) showed a high density, nearly random distribution and an inverse J-shaped size distribution. Species in group B (Quercus salicina, Quercus acuta, Quercus gilva) were distributed contagiously with conspicuous concentration of small trees (<5 cm dbh) around gaps. However, the species in this group included few trees likely to reach the canopy in the near future. Group C included fast-growing pioneer and shade intolerant species (e.g. Cornus controversa, Carpinus tschonoskii, Fagara ailanthoides), which formed large clumps. Most gaps were not characterized by successful regeneration of group B and C but did appear to accelerate the growth of group A. Group B species appear to require long-lived or large gaps while group C species require large, catastrophic disturbances, such as landslides, for regeneration.     

Mapping land cover and forest density in Zagros forests of Khuzestan province in Iran: A study based on Sentinel-2, Google Earth and field data

Zagros forests in western Iran have widely been destroyed because of various reasons. This study was performed to provide the land cover and forest density maps in Zagros forests of Khuzestan province using Sentinel-2, Google Earth and field data. The forest boundary in Khuzestan province was digitized in Google Earth. Sentinel-2 satellite images were provided for the study area. One 1:25000 index sheet of Iranian Mapping Organization (IMO) was selected as pilot area in the province. Sentinel-2 image of the pilot area was classified using different supervised classification algorithms to select the best algorithm for land cover mapping in Khuzestan province. In addition, to evaluate the accuracy of Google Earth data, field sampling was performed using random plots in different land covers. Field data of forest plots were applied to investigate tree canopy cover percent (forest density), as well. Classification of Sentinel-2 image in Zagros area of Khuzestan province was done using the best algorithm and the land cover was obtained. The forest density map was also obtained using a linear regression model between tree canopy cover percent (obtained from field plots) and normalized difference vegetation index (NDVI) (obtained from NDVI map). Finally, the accuracy of land cover map was assessed by some square plots on Google Earth. Results demonstrated that support vector machine (SVM) algorithm had the highest accuracy for land cover mapping. Results also showed that Google Earth images had a good accuracy in the Zagros forests of Khuzestan province. Results demonstrated that NDVI has been a good predicator to estimate tree canopy cover in the study area. Based on results, an area of 443,091.22 ha is covered by Zagros forests in Khuzestan province. Results of accuracy assessment of the land cover map showed the good accuracy of this map in Khuzestan province (overall accuracy: 91% and kappa index: 0.83). For optimum management of Zagros forests, it is suggested that the land cover and forest density mapping will be performed using SVM algorithm, NDVI, and Sentinel-2 satellite images in Zagros forests of Khuzestan province in the certain periods.     

Quaternary refugia in southwestern Iran: insights from two sympatric moth species (Insecta, Lepidoptera)

Alternating glacial and interglacial periods led to range shifts (contractions and expansions), persistence in distinct glacial refugia and extinction events in various temperate organisms. Today, the integrative analysis of molecular markers and spatial distribution models conducted for multiple taxa allows the detection of phylogeographical patterns, thus reconstructing major biogeographical events in their shared evolutionary history. In this study, the effects of past climate change on the evolutionary history of two sympatric moth species (Gnopharmia colchidaria s.l. and G. kasrunensis) and their host plants (Prunus scoparia and P. fenzliana) were inferred for the largely neglected biodiversity hot spot Iran. We complementarily analyzed the population structure of both moth species (187 specimens, based on COI) in congruence with batched species distribution models (SDMs) for all four taxa and for the times of the Last Glacial Maximum (21 ky BP), 6 ky BP and today. Coincidence of SDMs and the distribution of haplotype lineages indicated a shared refugium for the southwestern Zagros Mountains and potential species-specific refugial areas in the southern Caucasus and the Kope-Dagh Mountains. Both moth species experienced past population expansion.     

Holocene environmental change at Lake Shudu,Yunnan Province,southwestern China

A Holocene palaeorecord from Lake Shudu, Yunnan Province, southwestern China is dominated by (1) a pronounced basin-wide sedimentary hiatus after ca. 7.2 kcal yr BP, spanning some 4,000 years and (2) significant changes in sediment source/supply and an increase in heavy metal influx coupled with a shift to more eutrophic lake conditions from ca. 0.9 kcal yr BP, lasting ~300 years. The hiatus is most likely a due to a significant and abrupt reduction in sedimentation rates, the driver of which is unclear; although it appears likely to have been climatically driven. The environmental changes captured in the Lake Shudu palaeorecord provide unambiguous evidence of late Holocene anthropogenic activity, most likely linked to mining activity.     

Seroprevalence of Toxoplasma gondii infection in dogs in Sichuan Province, southwestern China

There is a lack of information concerning the prevalence of Toxoplasma gondii infection in dogs from southwestern China. In the present study, serum samples from 314 household dogs were collected from Wenchuan, Heishui, and Jiuzhaigou in Sichuan Province, southwestern China, in May and June 201; sera were assayed for T. gondii antibodies using an indirect haemagglutination test (IHA). Antibodies to T. gondii were found in 11 of 314 (3.5%), with IHA titers of 1:64 in 4 dogs, 1:128 in 3, 1:256 in 2, 1:512 in 1, and 1:1024 in 1. No regional difference was observed among the 3 counties (P > 0.05). The results of the present study indicated that infection with T. gondii in dogs is common in China, including household dogs in Sichuan Province, and should be of public health concern.     

云南省玉溪地区昆虫线虫资源分布调查及应用初探

昆虫线虫尤其是昆虫病原线虫可以部分替代化学农药防治有害昆虫。本文通过大蜡螟诱捕法对云南省玉溪市8县1区的昆虫线虫资源进行了调査。从270份土壤样品中分别得到57个昆虫线虫样本,分属于4个属,包括小杆科Rhabditidae的3个属,双胃科Diplogasteridae的1个属。结果表明,线虫的分布与土壤类型和植被类型密切相关,砂壤土、壤土中线虫检出率分别为100.0%、19.9%,较粘土12.1%的检出率高。在未受干扰的地块(林地和未耕地)线虫检出率为20.4%,受人类干扰的地块(烟田和农田)线虫检出率为16.1%,说明砂壤土和壤土适合昆虫线虫种群的建立,且在人活动较少的地块中线虫分布较多。用本土线虫在实验室条件下利用大蜡螟和蛴螬(棕色鳃金龟幼虫)对分离到的线虫进行致病力测定,结果表明:YN43(Oscheius sp.2)是大蜡螟最敏感的昆虫线虫品系,校正死亡率为76%;YN93(Oscheius sp.1)对蛴螬致病力最强,校正死亡率为54.5%,在防治云南地下害虫蛴螬方面具有很大的潜力。     

Odonata fauna of Khuzestan province (Iran) with a note on an Oriental species Trithemis aurora (Burmeister, 1839) as a new record for the south-west of Iran

This paper presents a survey of spider species that have not been previously recorded for Israel. Twenty species, twelve genera and two families (Mysmenidae and Phyxelididae) are recorded for the first time in Israel. Nine species, Agroeca parva Bosmans, 2011, Aulonia kratochvili Dunin et al., 1986, Ero flammeola Simon, 1881, Hogna ferox (Lucas, 1838), Maculoncus parvipalpus Wunderlich, 1995, Neon rayi (Simon, 1875), Pardosa aenigmatica Tongiorgi, 1966 and Phyxelida anatolica Griswold, 1990, are illustrated. Tarentula jaffa Strand, 1913, syn. n. is synonymised with Hogna ferox (Lucas, 1838), and Hahnia carmelita Levy, 2007, syn. n. is synonymised with Hahnia nava (Westring, 1851). A possible synonymy of the widespread Prodidomus rufus Hentz, 1847 with P. hispanicus Dalmas, 1919 known from the Iberian Peninsula is discussed. An original combination is restored for Trochosa urbana O.P.-Cambridge, 1876.     

中国云南省间座壳属两个新种

我国间座壳属真菌种类繁多,但对其分类研究滞后。通过对云南省间座壳属标本采集,基于形态学和核糖体内部转录间隔区、β微管蛋白基因、翻译延伸因子基因、钙调蛋白基因和组蛋白基因的DNA序列数据比较进行物种鉴定,描述了分别采集自八角黄皮和可可患病叶片上的间座壳属2个新种——八角黄皮间座壳和可可间座壳,并讨论了其与间座壳属物种的形态和系统发育关系。