共查询到20条相似文献,搜索用时 31 毫秒
1.
E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells 总被引:5,自引:0,他引:5
The homeobox gene Cdx1 is a regulator of intestinal epithelial cell proliferation and differentiation. Using a transfection approach, we showed here that the oncogenic activation of the beta-catenin pathway stimulates the endogenous expression of the Cdx1 mRNA as well as the activity of the Cdx1 promoter in cancer cells of the human colon. Reciprocally, the paralogue homeobox gene Cdx2 exerts an inhibitory effect on the basal and on the beta-catenin-stimulated activity of the Cdx1 promoter. The inhibitory effect of CDX2 requires the intact homeodomain. It is not dependent on canonical CDX binding sites in the Cdx1 promoter nor on the cis-elements specifically targeted by the beta-catenin/Tcf complex. We conclude that the oncogenically activated beta-catenin and CDX2 have opposite and independent effects on the Cdx1 homeobox gene. 相似文献
2.
The role of cadherin,beta-catenin,and AP-1 in retinoid-regulated carcinoma cell differentiation and proliferation 总被引:7,自引:0,他引:7
Shah S Pishvaian MJ Easwaran V Brown PH Byers SW 《The Journal of biological chemistry》2002,277(28):25313-25322
Vitamin A derivatives (retinoids) are potent regulators of cell proliferation and differentiation. Retinoids inhibit the function of the oncogenic AP-1 and beta-catenin/TCF pathways and also stabilize components of the adherens junction, a tumor suppressor complex. When treated with retinoic acid (RA), the breast cancer cell line, SKBR3, undergoes differentiation and reduction in cell proliferation. The present work demonstrates that in SKBR3 cells, which exhibit high AP-1 activity, RA-regulation of cadherin expression and function, but not changes in AP-1 (or beta-catenin/TCF) signaling, is responsible for the epithelial differentiation. However, cadherin function and recruitment of beta-catenin to the membrane is not required for RA to regulate DNA synthesis in these cells. RA also reduces the activity of an AP-1 and TCF-sensitive cyclin D1 reporter in SKBR3 cells in a manner that is independent of the TCF site. In contrast, in SW480 cells, which have high levels of beta-catenin/TCF signaling, the activity and retinoid responsiveness of the cyclin D1 promoter was markedly inhibited by mutation of the TCF site. These data indicate that the remarkably broad effects of RA on the growth and differentiation of many different epithelial cancers may well be explained by the ability of RA to differentially regulate the activity of RAR/RXR, AP-1, and beta-catenin/TCF pathways. 相似文献
3.
4.
5.
6.
The beta-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells 总被引:56,自引:0,他引:56
van de Wetering M Sancho E Verweij C de Lau W Oving I Hurlstone A van der Horn K Batlle E Coudreuse D Haramis AP Tjon-Pon-Fong M Moerer P van den Born M Soete G Pals S Eilers M Medema R Clevers H 《Cell》2002,111(2):241-250
7.
8.
9.
10.
11.
Rockman SP Currie SA Ciavarella M Vincan E Dow C Thomas RJ Phillips WA 《The Journal of biological chemistry》2001,276(48):45113-45119
12.
13.
14.
Vitamin D(3) promotes the differentiation of colon carcinoma cells by the induction of E-cadherin and the inhibition of beta-catenin signaling 总被引:13,自引:0,他引:13 下载免费PDF全文
Pálmer HG González-Sancho JM Espada J Berciano MT Puig I Baulida J Quintanilla M Cano A de Herreros AG Lafarga M Muñoz A 《The Journal of cell biology》2001,154(2):369-387
15.
16.
17.
18.
Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB 总被引:35,自引:0,他引:35
Batlle E Henderson JT Beghtel H van den Born MM Sancho E Huls G Meeldijk J Robertson J van de Wetering M Pawson T Clevers H 《Cell》2002,111(2):251-263
In the small intestine, the progeny of stem cells migrate in precise patterns. Absorptive, enteroendocrine, and goblet cells migrate toward the villus while Paneth cells occupy the bottom of the crypts. We show here that beta-catenin and TCF inversely control the expression of the EphB2/EphB3 receptors and their ligand ephrin-B1 in colorectal cancer and along the crypt-villus axis. Disruption of EphB2 and EphB3 genes reveals that their gene products restrict cell intermingling and allocate cell populations within the intestinal epithelium. In EphB2/EphB3 null mice, the proliferative and differentiated populations intermingle. In adult EphB3(-/-) mice, Paneth cells do not follow their downward migratory path, but scatter along crypt and villus. We conclude that in the intestinal epithelium beta-catenin and TCF couple proliferation and differentiation to the sorting of cell populations through the EphB/ephrin-B system. 相似文献
19.