共查询到20条相似文献,搜索用时 15 毫秒
1.
J. Hutchinson T. E. Miller J. Jahier K. W. Shepherd 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,64(1):31-40
Summary The chromosomes of the tetraploid wheats Triticum timopheevi (Genome AAGG) and T. araraticum (Genome AAGG) were C-banded at mitosis. The identity of the banded and unbanded chromosomes was then established by firstly making comparisons with the hexaploid species T. zhukovskyi which has the genome formula AAAAGG. Secondly, the meiotic pairing in F1 hybrids between T. timopheevi and diploid wheats was examined by means of C-banding. The results showed that the banded chromosomes belonged to the G genome, while the unbanded chromosomes belonged to the A genome. Only one of the two pairs of satellited chromosomes had strong heterochromatic bands. The relationship between the genomes of T. timopheevi and T. dicoccum (Genome AABB) was then assessed at meiosis in hybrids between these species, using the techniques of C-banding and in situ hybridisation of a cloned ribosomal RNA gene probe. It was concluded that there were differences both in the amount and distribution of heterochromatin and also translocation differences between the species. 相似文献
2.
E. D. Badaeva R. L. Boguslavsky N. S. Badaev A. V. Zelenin 《Plant Systematics and Evolution》1990,169(1-2):13-24
DifferentTriticum araraticum lines were studied by C-banding method. The intraspecific divergence ofT. araraticum was shown to be caused mainly by large chromosomal rearrangements. Two main chromosomal types were distinguished among the studied lines: (1) a karyotype similar to that ofT. timopheevii and (2) different one. The first type includes some lines ofT. araraticum subspp.kurdistanicum andararaticum; the second comprises most lines ofT. araraticum subsp.araraticum. The lines of the first type can give fertile F1 hybrids withT. timopheevii. 相似文献
3.
Dhaliwal H. S. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1977,50(5):235-239
Summary Electrophoretic profiles of crude protein extracts from seed of F1 hybrids and reciprocal crosses among diploid, tetraploid and hexaploid wheats were compared with those of their respective parental species. The electrophoretic patterns within each of three pairs of reciprocal crosses, T.boeoticum X T.urartu, T.monococcun X T. urartu and T.dicoccum X T. araraticum, were different from one another but were identical with those of their respective maternal parents. Protein bands characteristic of the paternal parents were missing in F1 hybrid seed suggesting that the major seed proteins in wheat were presumably regulated by genotype of the maternal parent rather than by the seed genotype. However, in another three pairs of reciprocal crosses, T.boeoticum X T. durum, T.dicoccum X T.aestivum and T. zhukovskyi x T. aestivum, protein bands attributable to the paternal parents were present in the F1 hybrid seeds indicating that the seed proteins were not always exclusively regulated by the maternal genotype. The expression of paternal genomes is presumably determined by dosage and genetic affinity of the maternal and paternal genomes in the hybrid endosperm. The maternal regulation of seed protein content is probably accomplished through the maternal control over seed size. The seed protein quality may, however, depend upon the extent of expression of the paternal genome. 相似文献
4.
Anther morphology of various species of the genus Triticum is consistent with previous evidence that different biotypes of T. boeoticum (AA) and T. urartu (BB) contributed the respective A and B genomes to the emmer (AeAeBeBe) and timopheevi (AtAtBtBt) tetraploid complexes. Anther length of T. dicoccoides (2.8 mm) and T. araraticum (3.0 mm) is mid-way between that of T. boeoticum (3.6) and T. urartu (2.2) and the same as that of the sterile hybrid T. boeoticum X T. urartu. With respect to mode of dehiscence, post anthesis reflexion of anther lobes and twisting of the anthers, T. dicoccoides resembles T. boeoticum, whereas T. araraticum resembles T. urartu. With respect to anther apex, T. dicoccoides resembles neither T. boeoticum nor T. urartu but is identical with their F1 hybrid. Among amphiploids involving four different Aegilops species and T. boeoticum lines, none could similarly account for the length or other diagnostic attributes of the tetraploid anthers. Anther morphology is consistent with the presumed genomic composition of the cultivated tetraploid and hexaploid wheats and seems to distinguish effectively the different genomic groups of the genus Triticum. 相似文献
5.
T. Terachi Y. Ogihara K. Tsunewaki 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(3):366-373
Summary Many related species and strains of common wheat were compared by matching differences among their mitochondrial genomes with their parent nuclear genomes. We examined three species of Aegilops, section Sitopsis (Ae. bicornis, Ae. sharonensis, and Ae. speltoides), emmer wheat (Triticum dicoccoides, T. dicoccum, and T. durum), common wheat (T. spelta, T. aestivum, and T. compaction), and timopheevi wheat (T. araraticum, T. timopheevi, and T. zhukovskyi). A single source of the cytoplasm was used in all the species, except Ae. speltoides (two sources), T. araraticum (two), and T. aestivum (three). Following restriction endonuclease analyses, the mitochondrial genomes were found to comprise seven types, and a dendrogram showing their genetic relatedness was constructed, based upon the percentage of common restriction fragments. MtDNAs from T. dicoccum, T. durum, T. aestivum, and T. compactum yielded identical restriction fragment patterns; these differed from T. dicoccoides and T. spelta mtDNAs in only 2.3% of their fragments. The fragment patterns of T. timopheevi and T. zhukovskyi were identical, and these differed from T. araraticum mtDNA by only one fragment. In both the emmer-dinkel and timopheevi groups, mitochondrial genome differentiation is evident, suggesting a diphyletic origin of each group. MtDNAs from four accessions of the Sitopsis species of Aegilops differ greatly from one another, but those of Ae. bicornis, Ae. sharonensis, and Ae. searsii, belonging to the same subsection Emarginata, are relatively similar. MtDNAs of timopheevi species are identical, or nearly so, to those of Ae. speltoides accession (09), suggesting that the latter was the cytoplasm donor to the former, polyploid group. The origin of this polyploid group seems to be rather recent in that the diploid and polyploid species possess nearly identical mitochondrial genomes. We cannot determine, with precision, the cytoplasm donor to the emmer-dinkel group. However, our results do suggest that mitochondrial DNAs show larger evolutionary divergence than do the ctDNAs from these same strains.Contribution no. 507 from the Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan 相似文献
6.
A. Michael Powell 《American journal of botany》1972,59(7):760-768
Artificial hybridizations were conducted with 37 species of four genera of the subtribe Peritylanae (Compositae). Crossability, pollen stainability, and meiotic behavior were examined for most of the resulting 61 F1 hybrid combinations. Intergeneric hybrids involving Amauria, Eutetras, Pericome, and Perityle, and infrageneric hybrids involving Perityle were readily obtained. Lower crossability and hybrid fertility were displayed by intergeneric crosses. The infrageneric crosses of Perityle were organized into two categories, intersectional and infrasectional, based upon the taxonomic sections that are recognized for the genus; sect. Pappothrix, sect. Laphamia, and sect. Perityle. In general, the crossability and hybrid fertility of intersectional crosses was markedly higher than that of intergeneric crosses. The results suggest a closer relationship between the three taxonomic sections of Perityle than between Perityle and other genera of the subtribe. 相似文献
7.
N. Mori Y. -G. Liu K. Tsunewaki 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(1):129-134
Intra- and inter-specific variations in the nuclear DNA of Triticum dicoccoides Körn. (2n = 28, genome constitution AABB) and T. araraticum Jakubz. (2n = 28, AAGG), wild species, respectively, of the Emmer and Timopheevi group, were studied by restriction fragment length polymorphism (RFLP) analysis. Total DNAs of 32 T. dicoccoides and 24 T. araraticum accessions, collected from throughout the distribution areas of these species, were treated with two 6-bp cutters and hybridized with 30 nuclear DNA clones as probes to detect RFLPs. A total of 167 hybrid bands were observed per accession. All the enzyme-probe combinations showed RFLPs between accessions. The average genetic distance between the T. dicoccoides accessions was 0.0135 ± 0.0031 and that between the T. araraticum accessions 0.0036 ± 0.0015, indicative of about a four-fold intraspecific variation in T. dicoccoides as compared to T. araraticum in terms of genetic distance. No significant genetic differentiation was found for the geographical populations of these species, the genetic distance between the two species being 0.0482 ± 0.0022. The interspecific divergence corrected for intraspecific divergence was 0.0395, about three times that for T. dicoccoides and 11 times that for T. araraticum. The results show that in the wild state the Emmer and Timopheevi groups are clearly differentiated and that T. dicoccoides has much greater variation than T. araraticum, suggesting a relatively recent origin for the latter and therefore a diphyletic origin for these species.Contribution from the Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan, No. 535 相似文献
8.
Ion concentrations were measured in the leaves of synthetichexaploid wheats and their parents growing in saline hydroponicculture. The synthetic hexaploids contained genomes from a tetraploidwheat (Triticum diccocum, T. durum, T. araraticum or T. timopheevi)and from a diploid species (T. monococcum, T. urartu, T. boeoticumor Aegilops squarrosa). Leaf Na concentrations were low, andK concentrations high, in Ae. squarrosa, T. araraticum and allof the synthetic hexaploids, but high in T. dicoccum and T.durum. At low salinities leaf Na concentrations were particularlyhigh in T. durum in comparison with the other species. Theseresults suggest that the enhanced K/Na discrimination character,originally found in Ae. squarrosa and BBAADD genome hexaploidwheats, is also present in diploid wheat and in GGAA genometetraploid wheats. It is suggested that this character has beenlost in the evolution of the BBAA genome tetraploid wheats. Key words: Salt, ion transport, A genome, Triticum spp 相似文献
9.
Crosses were made in all combinations of the six diploid species of the Solarium nigrum complex that occur in North America. Some interspecific pollinations failed to yield viable seed; successful crosses gave rise to moderately to highly sterile F1 hybrids. Results of interspecific crosses suggested phylogenetic relationships that were not completely in accord with those suggested by morphology. Interspecific crosses also gave varied results. All interpopulational crosses within S. interius and S. sarachoides produced fully fertile hybrids. In contrast, hybrids within S. americanum and S. douglasii varied from fully fertile to almost completely sterile. Populations of S. pseudogracile could be divided into two groups which are geographically separated but not morphologically differentiated. Fully fertile hybrids resulted from crosses within a group, whereas crosses between groups gave hybrids with reduced fertility. Four crossing groups were observed within 5. nodiflorum; three of the parental groups are sympatric and are morphologically differentiated. Although hybrid sterility in interspecific crosses is sometimes used to support delimitation of species, the presence of sterility in intraspecific crosses suggests that such an interpretation is unwarranted for the S. nigrum complex. Hybrid sterility, therefore, is not considered to have special taxonomic significance in this complex. 相似文献
10.
K. K. Pandey 《American journal of botany》1962,49(8):874-882
Pandey , K.K. (Crop Res. Div., D.S. & I.R., Lincoln, Christchurch, New Zealand.) Interspecific incompatibility in Solanum species. Amer. Jour. Bot. 49(8): 874–882. Illus. 1962.—A diallel cross involving 11 self-incompatible and 3 self-compatible species of Solanum was made to study the genetic basis of interspecific incompatibility. Interspecific incompatibility was not limited to crosses in which a self-compatible species was used as the male parent onto a self-incompatible species (unilateral incompatibility). A number of crosses between self-incompatible species were incompatible. In one cross, Q vernei X verrucosum, a self-compatible species was successful as a pollen parent with a self-incompatible species. Unlike other hybrids between self-compatible and self-incompatible species, which are self-incompatible, these F1 hybrids were self-fertile, and cross-fertile among themselves and with both parents. The self-fertile S. polyadenium was cross-incompatible as a female as well as a male parent with all other species. It is suggested that the unilateral incompatibility is a property of the allele SC which originated as a consequence of one kind of breakdown of the SI gene; the SC allele produces “bare” pollen growth substances which are inactivated in an incompatible style. It is proposed that the failure of the principle of unilateral interspecific incompatibility in solanaceous species may be due to the action of alleles at the second incompatibility locus revealed in certain Mexican species. It is assumed that the South American species are selected intraspecifically only for the action of S alleles but that in certain interspecific crosses and rarely in intraspecific crosses the alleles at the second locus may be expressed, thus interfering with the usual action of S alleles. The F1 hybrids Q verrucosum (self-fertile) X simplicifolium (self-sterile) were self-incompatible at the tetraploid as well as the diploid level, and their cross-compatibility behavior was consistent with the expected activity of the SC and SI alleles of the 2 parents respectively. 相似文献
11.
Shahina A. Ghazanfar 《Plant Systematics and Evolution》1989,168(3-4):143-148
Ten species in the genusSilene sectt.Siphonomorpha andAuriculatae were crossed artificially involving 612 crosses to test inter- and infraspecific, intervarietal and intersectional crossability. In sect.Siphonomorpha all interspecific crosses (between diploids) failed due to cross- or seed-incompatibility; however, intervarietal crosses betweenS. gigantea var.gigantea andS. gigantea var.incana produced hybrids. In sect.Auriculatae hybrids were produced betweenS. vallesia andS. boryi, both tetraploids, but crosses between these and the diploid species were unsuccessful. The delimitation and status of the species in both sections was supported by the crossing results. 相似文献
12.
Patrick A. Vigueira Jacob F. Schaefer David D. Duvernell Brian R. Kreiser 《Evolutionary ecology》2008,22(1):55-70
We assessed prezygotic (probability of spawning) and postzygotic (hatching success) reproductive isolation among the three
ecologically and morphologically similar species in the Fundulus notatus species complex. We employed a multi-generation breeding experiment to test the hypotheses that karyotypic differences, body
size differences, or geographic isolation among populations will increase pre or postzygotic reproductive barriers. Overall,
prezygotic barriers were strong and postzygotic barriers weak in crosses of non-hybrid heterospecifics (F1 hybrid crosses)
while prezygotic barriers were weaker and postzygotic barriers stronger in crosses involving hybrid individuals (F2 hybrid
crosses and backcrosses). Prezygotic barriers among the two smaller species (Fundulus notatus and F. euryzonus) broke down rapidly; first generation hybrids spawned (F2 hybrid crosses and backcrosses) as frequently as parental forms
in intraspecific crosses. There was no increase in postzygotic barriers among species with cytogenetic differences. There
were increased prezygotic, but not postzygotic, barriers among geographically isolated populations of one species. While pure
males and females were just as likely to spawn with hybrids, some types of hybrid females suffered from increased sterility,
but not inviability, over hybrid males. Female sterility was only seen in hybrids with a Fundulus euryzonus parent, while other female hybrids produced viable eggs. 相似文献
13.
Drosophila mojavensis and Drosophila arizonae are cactophilic flies that have been used extensively in speciation studies. Incomplete premating isolation, evidence of reinforcement, and a lack of recent introgression between these species point to a potentially important role for post‐zygotic isolating barriers in this system. Other than hybrid male sterility, however, post‐zygotic isolation between D. mojavensis and D. arizonae has received little attention. In this study, we examined viability and life‐history traits of D. mojavensis/D. arizonae F1 hybrids from sympatric crosses. Specifically, we reared hybrids and purebreds on the natural host cacti of each parental species and compared viability, development time, thorax length, and desiccation resistance between hybrids and purebreds. Interestingly, hybrid females from both crosses performed similarly or even better than purebred females. In contrast, hybrid sons of D. arizonae mothers, in addition to being sterile, had shorter average thorax length than males of both parental species, and hybrid males from both crosses had substantially lower desiccation resistance than D. mojavensis males. The probable cost to hybridization for D. mojavensis females resulting from reduced desiccation resistance of hybrid sons may have been an important selective factor in the history of reinforcement for crosses involving these females. 相似文献
14.
Michel Solignac 《Oecologia》1976,26(1):33-52
Summary A demographic study was carried out on two closely related species of the isopods Jaera (albifrons) ischiosetosa and J. (a.) albifrons and their F
1 hybrids. The results from the 16 possible combinations of crosses have permitted an analysis of the nature of the mechanisms assuring the isolation of the species studied. Although intraspecific crosses yield an immediate success, interspecific crosses in the absence of choice of mates progress only slowly during the course of weeks. The results of both crosses between hybrids and back-crosses turn out to be intermediate between those of intra- and interspecific crosses. The hybrids of the first generation are perfectly viable and their survival curves are identical to those of the parents. The fertility of parents in intra- and interspecific crosses is comparable, with the exception of the fragility of female descendants (heterogametic sex) in one direction of crossing. The fertility of the F
1 hybrids, however, crossed either among themselves or with their parents, is quite noticeably decreased: the time needed to double the size of the population is 2.5 times longer for the hybrids than for the parents. This hybrid breakdown completes the pre-fertilization isolating mechanisms: partial ecological isolation, and especially ethological isolation, is practically total when a choice of mates exists. The two species studied, for which demographic parameters are quite close, were raised together for ten generations and yielded only exceptional hybrids with a frequency which does not exceed that found under natural conditions. 相似文献
15.
The diploid wheats Triticum boeoticum and T. urartu are sympatric with one another throughout the geographic range of the wild tetraploids. Reciprocal crosses between ecogeographic types within each diploid species gave viable seed, but interspecific crosses consistently gave viable seed only when T. boeoticum was the female parent. Apparently urartu cytoplasm in combination with the boeoticum genome resulted in nonviable seed. The endosperm failed to develop normally despite regular endosperm fertilization. The F1 plants obtained were completely self sterile although they showed regular intergenomic pairing (7II) at meiosis. Presumably the accumulation of cryptic differences between the two closely related genomes under reproductive isolation accounts for this sterility. The same accumulated cryptic differences could largely account for the preferential diploid pairing in the tetrapolid wheats which presumably were derived from such hybrids by chromosome doubling. The behavior of reciprocal crosses between the diploids and tetraploids suggested that T. boeoticum contributed the cytoplasm to both of the wild tetraploid species. 相似文献
16.
TheNor-loci of polyploid wheats and their putative diploid progenitor species were assayed by probing isolated nuclear DNA with ribosomal DNA spacer sequences (spacer rDNA sequences, isolated by cloning), from theNor-loci of genomes B (Triticum aestivum), G (T. timopheevi), B (syn. S,T. speltoides), A (T. monococcum) and V (Dasypyrum villosum). DNA samples for analysis were digested with the restriction endonuclease Taq 1 and assayed by DNA-DNA hybridization under standard (37°C) and high stringency (64°C) conditions. The assay procedure emphasized differences between the divergent spacer sequences of the polyploid species and allowed relative homologies to the respective sequences in diploid species to be established. — The studies indicated thatT. timopheevi andT. speltoides contain different sets of spacer rDNA sequences which were readily distinguishable and, in the case ofT. timopheevi, assigned toNor-loci on different chromosomes. This contrast with the spacer rDNA sequences of the majorNor-loci on chromosomes 1 B and 6 B inT. aestivum, which were difficult to distinguish and were deduced to contain very similar sequences. Among the diploid progenitor species only the spacer rDNA fromT. speltoides shared close homology with polyploid wheat species. OneNor-locus inT. timopheevi (on chromosome 6 G) did not show close homology with any of the rDNA spacer probes available. — The data suggestsT. speltoides was the origin of someNor-loci for both theT. timopheevi andT. turgidum lines of tetraploid wheats. The possibility that the 6GNor-locus inT. timopheevi may have derived from an unknown diploid species by introgressive hybridization is discussed. The spacer rDNA sequence probe fromT. monococcum shared good homology with some accessions ofD. villosum and a line ofT. dicoccoides; the implications of this finding for evolution of present-day wheats are discussed. 相似文献
17.
Ahmad Hashemi Ali Estilai Jan E. West J. Giles Waines 《American journal of botany》1988,75(2):197-204
ABSTRACT Caespitose and cold-tolerant plants of Parthenium ligulatum (Jones) Barneby (Asteraceae) from a native population in the Uinta Basin, Utah, were uprooted, potted, and transferred to a greenhouse in California. Approximately two years after transfer, the plants flowered and subsequently were crossed to diploid guayule (Parthenium argentatum Gray), the rubber-bearing species, native to the state of Durango, Mexico. Only female guayule × male P. ligulatum crosses produced F1 hybrids. Only crosses involving guayule as female parent and F1 plants as male parents produced backcross (BC,) plants. Hybrid plants were variable with respect to their growth habit, inflorescence, and leaf shape. Both parents and F1 hybrids had 2n = 36 chromosomes. Unlike the parents, however, meiosis was irregular in the hybrids which showed a range of 0–5 and an average of 2.1 univalents at metaphase I. Hybrids averaged 0.87 laggards at anaphase I and 0.83 micronuclei at the tetrad stage. The crossability of guayule and P. ligulatum, the high degree of chromosome pairing of the F1 hybrids, and the production of BC1 plants indicate that the two species are related in spite of their distinct morphological and ecological differences. This study suggests that the cold-tolerance trait of P. ligulatum may be transferred to guayule through interspecific hybridization followed by backcrossing. The development of cold-tolerant guayule cultivars is expected to expand the areas of guayule production beyond that of the Chihuahuan desert and similar climates. 相似文献
18.
Daniel J. Crawford Jenny K. Archibald Mark E. Mort Arnoldo Santos-Guerra 《Plant Systematics and Evolution》2009,279(1-4):103-113
Pollen fertility was determined for synthetic F1 hybrids between members of the genus Tolpis endemic to the Canary Islands. Mean fertility varies from 1 to nearly 100%. High hybrid sterility is unusual for island lineages. Surprisingly, the fertility of hybrids between morphologically distinct species was generally higher than some crosses within the morphologically variable group that includes the Tolpis laciniata and T. lagopoda complexes. Within this group, fertility was lower particularly in hybrids involving one recognized segregate species (T. webbii), but none of the four potentially new species had reduced hybrid fertility. Despite the overall fertility differences between groupings within the T. laciniata and T. lagopoda complexes there is variation in fertility within groups; sterility factors have a complex inter-populational distribution. The cause of hybrid sterility is unknown, but preliminary data suggest that both chromosomal and genetic factors are involved. 相似文献
19.
S. W. Hussain W. M. Williams 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):678-690
Trifolium ambiguum M. Bieb and T. repens L. are taxonomically related but very difficult to cross. The rare hybrids so far reported between these two species were
obtained only by embryo culture. This difficulty has been overcome in the present research by the creation of a “fertile bridge”
between T. ambiguum and T. repens. Characters of interest can now be transferred from T. ambiguum to T. repens by using this “fertile bridge” without the use of sophisticated techniques. An array of backcross progenies was generated
from crosses between a T. ambiguum×T. repens F1 hybrid (8x H-435) and its parental species. The 8x hybrid was cross-fertile only with T. repens and resulted in 145 seeds from 1578 reciprocal crosses. Eleven of nineteen initially grown BC1F1 plants were all hexaploid with an average pollen stainability of 41.6%. A high frequency of multivalents at metaphase-I indicated
that both autosyndetic and allosyndetic pairing occurred. Backcrosses of 6x BC1F1 plants to T. repens resulted in 5x BC2F1 plants with an average pollen stainability of 59.3%. On the other hand, 6x BC1F1×6x T. ambiguum crosses did not produce any seed and only two pentaploid plants were obtained from 6x BC1F1×4x T. ambiguum crosses. The difficulty encountered in generating 6x backcross progeny with 6x T. ambiguum was overcome by intercrossing the 6x BC1F1 plants and producing 6x BC1F2 plants with an average pollen stainability of 65.8%. One of these 6x BC1F2 plants was cross-compatible as a female with 6x T. ambiguum and resulted in CBC2 plants that were all cross-compatible with 6x T. ambiguum. The 6x BC1F2 plants are likely to be superior to 6x BC1F1 progeny, as they have exhibited better expression of the combined rhizomatous and stoloniferous growth habit, improved fertility,
more frequent nodal rooting and heavier nodulation. Consequently, the 6x BC1F2 plants can either be used directly in the selection programme or as a “fertile bridge” between the two parental species.
The present work has resulted in the development of a series of fertile hybrids by the manipulation of chromosome numbers,
combining the agronomic characteristics of the parent species in varying genome balances and at a range of ploidy levels.
It is concluded that the initial sterility of the primary interspecific hybrids need not be a barrier to successful inter-breeding.
Received: 2 August 1996 / Accepted: 4 April 1997 相似文献
20.
Keisuke Atsumi Kazuhiro Nomoto Yoshiyasu Machida Masaki Ichimura Itsuro Koizumi 《Ichthyological Research》2018,65(1):165-167
Egg hatching rates of F1 hybrids among three fishes, Japanese dace Tribolodon hakonensis, Pacific redfin T. brandtii, and rosyface dace T. sachalinensis, were compared with pure crosses. They are highly divergent (diverged 10–20 million years ago) but naturally hybridizing. Although crosses involving female rosyface dace were not available, hatching success was high among all combinations of hybrids (76 ± 23%) and comparable to pure crosses (86 ± 8%), implying ecological or genetic isolation mechanisms maintaining each species in later life stages. 相似文献