共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
DNA marker analysis of loci conferring resistance to peanut root-knot nematode in soybean 总被引:4,自引:0,他引:4
J. P. Tamulonis B. M. Luzzi R. S. Hussey W. A. Parrott H. R. Boerma 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):664-670
Peanut root-knot nematode [Meloidogyne arenaria (Neal) Chitwood] (Ma) is a serious pathogen of soybean, Glycine max L. Merrill, in the southern USA. Breeding for root-knot nematode resistance is an important objective in many plant breeding
programs. The inheritance of soybean resistance to Ma is quantitative and has a moderate-to-high variance-component heritability
on a family mean basis. The objectives of the present study were to use restriction fragment length polymorphism (RFLP) markers
to identify quantitative trait loci (QTLs) conferring resistance to Ma and to determine the genomic location and the relative
contribution to resistance of each QTL. An F2 population from a cross between PI200538 (Ma resistant) and ‘CNS’ (Ma susceptible) was mapped with 130 RFLPs. The 130 markers
converged on 20 linkage groups spanning a total of 1766 cM. One hundred and five F2:3 families were grown in the greenhouse and inoculated with Ma Race 2. Two QTLs conferring resistance to Ma were identified
and PI200538 contributed the alleles for resistance at both QTLs. One QTL was mapped at 0-cM recombination with marker B212V-1
on linkage group-F (LG-F) of the USDA/ARS-Iowa State University RFLP map, and accounted for 32% of the variation in gall number.
Another QTL was mapped in the interval from B212D-2 to A111H-2 on LG-E, and accounted for 16% of the variation in gall number.
Gene action for the QTL located on LG-F was additive to partially dominant, whereas the gene action for the QTL on LG-E was
dominant with respect to resistance. The two QTLs, when fixed on the framework map, accounted for 51% of the variation in
gall number in a two-QTL model. The two QTLs for Ma resistance were found in duplicated regions of the soybean genome, and
the major QTL for Ma resistance on LG-F is positioned within a cluster of eight diverse disease-resistance loci.
Received: 10 June 1996 / Accepted: 18 April 1997 相似文献
3.
QTL Mapping for Frond Length and Width in Laminaria japonica Aresch (Laminarales, Phaeophyta) Using AFLP and SSR Markers 总被引:1,自引:0,他引:1
Fuli Liu Zhanru Shao Haining Zhang Jidong Liu Xiuliang Wang Delin Duan 《Marine biotechnology (New York, N.Y.)》2010,12(4):386-394
In Laminaria japonica Aresch breeding practice, two quantitative traits, frond length (FL) and frond width (FW), are the most important phenotypic
selection index. In order to increase the breeding efficiency by integrating phenotypic selection and marker-assisted selection,
the first set of QTL controlling the two traits were determined in F2 family using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Two prominent L. japonicas inbred lines, one with “broad and thin blade” characteristics and another with “long and narrow blade” characteristics, were
applied in the hybridization to yield the F2 mapping population with 92 individuals. A total of 287 AFLP markers and 11 SSR markers were used to construct a L. japonica genetic map. The yielded map was consisted of 28 linkage groups (LG) named LG1 to LG28, spanning 1,811.1 cM with an average
interval of 6.7 cM and covering the 82.8% of the estimated genome 2,186.7 cM. While three genome-wide significant QTL were
detected on LG1 (two QTL) and LG4 for “FL,” explaining in total 42.36% of the phenotypic variance, two QTL were identified
on LG3 and LG5 for the trait “FW,” accounting for the total of 36.39% of the phenotypic variance. The gene action of these
QTL was additive and partially dominant. The yielded linkage map and the detected QTL can provide a tool for further genetic
analysis of two traits and be potential for maker-assisted selection in L. japonica breeding. 相似文献
4.
J. N. A. M. Rouppe van der Voort H. J. van Eck P. M. van Zandvoort H. Overmars J. Helder J. Bakker 《Molecular & general genetics : MGG》1999,261(6):1021-1031
A mapping strategy is described for the construction of a linkage map of a non-inbred species in which individual offspring
genotypes are not amenable to marker analysis. After one extra generation of random mating, the segregating progeny was propagated,
and bulked populations of offspring were analyzed. Although the resulting population structure is different from that of commonly
used mapping populations, we show that the maximum likelihood formula for a normal F2 is applicable for the estimation of
recombination. This “pseudo-F2” mapping strategy, in combination with the development of an AFLP assay for single cysts, facilitated
the construction of a linkage map for the potato cyst nematode Globodera rostochiensis. Using 12 pre-selected AFLP primer combinations, a total of 66 segregating markers were identified, 62 of which were mapped
to nine linkage groups. These 62 AFLP markers are randomly distributed and cover about 65% of the genome. An estimate of the
physical size of the Globodera genome was obtained from comparisons of the number of AFLP fragments obtained with the values for Caenorhabditis elegans. The methodology presented here resulted in the first genomic map for a cyst nematode. The low value of the kilobase/centimorgan
(kb/cM) ratio for the Globodera genome will facilitate map-based cloning of genes that mediate the interaction between the nematode and its host plant.
Received: 7 January 1999 / Accepted: 16 April 1999 相似文献
5.
Salmaso M Malacarne G Troggio M Faes G Stefanini M Grando MS Velasco R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(8):1129-1143
Grapevine molecular maps based on microsatellites, AFLP and RAPD markers are now available. SSRs are essential to allow cross-talks
between maps, thus upgrading any growing grapevine maps. In this work, single nucleotide polymorphisms (SNPs) were developed
from coding sequences and from unique BAC-end sequences, and nested in a SSR framework map of grapevine. Genes participating
to flavonoids metabolism and defence, and signal transduction pathways related genes were also considered. Primer pairs for
351 loci were developed from ESTs present on public databases and screened for polymorphism in the “Merzling” (a complex genotype
Freiburg 993–60 derived from multiple crosses also involving wild Vitis species) × Vitis vinifera (cv. Teroldego) cross population. In total 138 SNPs, 108 SSR markers and a phenotypic trait (berry colour) were mapped in
19 major linkage groups of the consensus map. In specific cases, ESTs with putatively related functions mapped near QTLs previously
identified for resistance and berry ripening. Genes related to anthocyanin metabolism mapped in different linkage groups.
A myb gene, which has been correlated with anthocyanin biosynthesis, cosegregated with berry colour on linkage group 2. The possibility
of associating candidate genes to known position of QTL is discussed for this plant.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Marzia Salmaso and Giulia Malacarne contributed equally to the present work. 相似文献
6.
Fernando J. Yuste-Lisbona Carmen Capel Emilio Sarria Rocío Torreblanca María L. Gómez-Guillamón Juan Capel Rafael Lozano Ana I. López-Sesé 《Molecular breeding : new strategies in plant improvement》2011,27(2):181-192
Powdery mildew caused by Podosphaera xanthii has become a major problem in melon since it occurs all year round irrespective of the growing system. The TGR-1551 melon
genotype was found to be resistant to several melon diseases, among them powdery mildew. However, the corresponding resistance
genes have been never mapped. We constructed an integrated genetic linkage map using an F2 population derived from a cross
between the multi-resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’. The map spans 1,284.9 cM,
with an average distance of 3.6 cM among markers, and consists of 354 loci (188 AFLP, 39 RAPD, 111 SSR, 14 SCAR/CAPS/dCAPS,
and two phenotypic traits) distributed in 14 linkage groups. QTL analysis identified one major QTL (Pm-R) on LG V for resistance to races 1, 2, and 5 of powdery mildew. The PM4-CAPS marker is closely linked to the Pm-R QTL at a genetic distance of 1.9 cM, and the PM3-CAPS marker is located within the support interval of this QTL. These codominant
markers, together with the map information reported here, could be used for melon breeding, and particularly for genotyping
selection of resistance to powdery mildew in this vegetable crop species. 相似文献
7.
Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map 总被引:10,自引:0,他引:10
F. Dunemann R. Kahnau I. Stange 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1146-1155
A molecular linkage map of Rhododendron has been constructed by using a segregating population from an interspecific cross. Parent-specific maps based on 239 RAPD,
38 RFLP, and two microsatellite markers were aligned using markers heterozygous in both parents. The map of the male parent
‘Cunningham’s White’ comprised 182 DNA markers in 13 linkage groups corresponding to the basic chromosome number. In the female
parent ‘Rh 16’ 168 markers were located on 18 linkage groups. An assignment of putative homologous linkage groups was possible
for 11 groups of each parent. QTL analyses based on the non-parametric Kruskal-Wallis rank-sum test were performed for the
characters “leaf chlorosis” and “flower colour” scored as quantitative traits. For leaf chlorosis, two genomic regions bearing
QTLs with significant effects on the trait were identified on two linkage groups of the chlorosis-tolerant parent. RAPD marker
analysis of additional lime-stressed genotypes tested under altered environmental conditions verified the relationship between
marker allele frequencies and the expression of chlorosis. Highly significant QTL effects for flower colour were found on
two chromosomes indicating major genes located in these genome areas. The prospects for utilization of a linkage map in Rhododendron are discussed.
Received: 28 September 1998 / Accepted: 5 November 1998 相似文献
8.
Holger Budahn Herbert Peterka Magdi Ali Ahmed Mousa Yunhua Ding Shaosong Zhang Jinbin Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):775-782
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil
radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration
to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition
of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated
markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted
of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome
d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed
as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish
linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible).
A dominant major QTL Hs1
Rph
explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments
to incorporate segments carrying desired genes as Hs1
Rph
from radish into rapeseed by means of chromosome addition lines. 相似文献
9.
Kurt O Yilmaz-Aydogan H Uyar M Isbir T Seyhan MF Can A 《Molecular biology reports》2012,39(6):6723-6730
It has been suggested that the estrogen receptor alpha (ERα) and vitamin D receptor (VDR) genes as possibly implicated in reduced bone mineral density (BMD) in osteoporosis. The present study investigated the relation
of ERα PvuII/XbaI polymorphisms and VDR FokI/TaqI polymorphisms with BMD in Turkish postmenopausal women. Eighty-one osteoporotic and 122 osteopenic postmenopausal women
were recruited. For detection of the polymorphisms, polymerase chain reaction-restriction fragment lenght polymorphism techniques
have been used. BMD was measured at the lumbar spine and hip by dual-energy X-ray absorptiometry. Distributions of ERα (PvuII dbSNP: rs2234693, XbaI dbSNP: rs9340799) and VDR genotypes (FokI dbSNP rs10735810, TaqI dbSNP: rs731236) were similar in study population. Although overall prevalence of osteoporosis had no association with these
genotypes, the prevalence of decreased femoral neck BMD values were higher in the subjects with ERα PvuII “PP” and ERα
XbaI “XX” genotypes than in those with “Pp/pp” genotypes and “xx” genotype, respectively (P < 0.05). Furthermore, subjects with VDR FokI “FF” genotype had lower BMD values of femoral neck and total hip compared to those with “Ff” genotype (P < 0.05). In the logistic regression analysis, we confirmed the presence of relationships between the VDR
FokI “FF” genotypes, BMI ≤ 27.5, age ≥ 55 and the increased risk of femoral neck BMD below 0.8 value in postmenopausal women.
The present data suggests that the ERα PvuII/XbaI and VDR FokI polymorphisms may contribute to the determination of bone mineral density in Turkish postmenopausal women. 相似文献
10.
Anne M. Rae Matthieu P. C. Pinel Catherine Bastien Maurizio Sabatti Nathaniel R. Street James Tucker Caroline Dixon Nicolas Marron Sophie Y. Dillen Gail Taylor 《Tree Genetics & Genomes》2008,4(1):97-112
Populus is a genus of fast growing trees that may be suitable as a bioenergy crop grown in short rotation, but understanding the
genetic nature of yield and genotype interactions with the environment is critical in developing new high-yield genotypes
for wide-scale planting. In the present study, 210 genotypes from an F2 population (Family 331; POP1) derived from a cross between Populus trichocarpa 93-968 and P. deltoides ILL-129 were grown in southern UK, central France and northern Italy. The performance of POP1, based upon first- and second-year
main stem traits and biomass production, improved from northern to southern Europe. Trees at the Italian site produced the
highest mean biomass ranging from 0.77 to 18.06 oven-dried tonnes (ODT) ha−1 year−1, and the UK site produced the lowest mean biomass ranging from 0.18 to 10.31 ODT ha−1 year−1. Significant genotype × environment interactions were seen despite heritability values across sites being moderate to high.
Using a pseudo-testcross analysis, 37 quantitative trait loci (QTL) were identified for the maternal parent and 45 for the
paternal parent for eight stem and biomass traits across the three sites. High genetic correlations between traits suggested
that collocating QTL could be inferred as a single pleiotropic QTL, reducing the number of unique QTL to 23 and 24 for the
maternal and paternal parent, respectively. Additive genetic effects were seen to differ significantly for eight QTL on the
maternal map and 20 on the paternal map across sites. An additive main effects and multiplicative interaction analysis was
carried out to obtain stability parameters for each trait. These parameters were mapped as QTL, and collocation to trait QTL
was accessed. Two of the eight stability QTL collocate to trait QTL on the maternal map, and 8 of the 20 stability QTL collocate
to trait QTL on the paternal map, suggesting that a regulatory gene model is prevalent over an allele sensitivity model for
stem trait stability across these environments. 相似文献
11.
M. H. Tixier P. Sourdille G. Charmet G. Gay C. Jaby T. Cadalen S. Bernard P. Nicolas M. Bernard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1076-1082
An intervarietal molecular-marker map was used for the detection of genomic regions influencing crossability between wheat
(Triticum aestivum L. em Thell) and rye (Secale cereale L.). Analysis of deviance and logistic marker-regression methods were conducted on data from doubled haploid lines from a
cross between “Courtot” and “Chinese Spring”. A major quantitative trait locus (QTL) involved in crossability, associated
with the marker Xfba367-5B, was detected on the short arm of chromosome 5B. An additional locus, Xwg583-5B, was indicated on the long arm of chromosome 5B. This minor QTL might correspond to Kr1 which was presumed to be the major gene controlling crossability. Another locus of the genome, Xtam51-7A on chromosome 7A, was significantly associated with this trait. Alleles of “non-crossability” were contributed by the non-crossable
cultivar “Courtot”. The three-marker model explains 65% of the difference in crossability between the two parents. The present
results are discussed in relation to those previously carried out to locate the Kr genes by using the telocentric mapping technique.
Received: 27 February 1998 / Accepted: 15 May 1998 相似文献
12.
Lowe I Jankuloski L Chao S Chen X See D Dubcovsky J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(1):143-157
A mapping population of 186 recombinant inbred lines developed from a cross between UC1110, an adapted California spring wheat,
and PI610750, a synthetic derivative from CIMMYT’s Wide Cross Program, was evaluated for its response to current California
races of stripe rust (Puccinia striiformis f. sp. tritici) in replicated field trials over four seasons (2007–2010) in the northern Sacramento Valley. A genetic map was constructed
consisting of 1,494 polymorphic probes (SSRs, DArTs, and ESTs) mapped to 558 unique loci, and QTL analysis revealed the presence
of four stripe rust resistance QTL segregating in this population, two from UC1110 (on chromosomes 3BS and 2BS) and two from
PI610750 (5AL and 2AS). The two QTL of largest effects (on 3BS and 5AL) were validated in independent populations and their
intervals narrowed to 2.5 and 5.3 cM, respectively. The 3BS QTL was shown, by allelism test and genotype, to carry a gene
different from the Yr30/Sr2 complex. Mapped position also suggests that the 3BS QTL is associated with a gene different from either Yrns-B1 or YrRub, two stripe rust resistance genes mapped to this region in other studies. The 5AL QTL carries a previously unreported partial
stripe rust resistance gene, designated here as Yr48. This paper discusses the individual contributions to resistance of these four QTL, their epistatic interactions, and their
potential in durable resistance breeding strategies based on combinations of partial resistance genes. 相似文献
13.
The green peach aphid (GPA), Myzus persicae (Sulzer), is a widespread pest insect that significantly reduces yield in peach orchards [Prunus persica (L.) Batsch]. Chemical control of the GPA population in the orchards showed little efficiency because of the development
of resistance to most classes of insecticides. Biological control partially gave convincing results. Breeding for resistant
peach cultivars is therefore a serious option to take into account for the development of sustainable pest management. Among
the few available resistance cultivars, the rootstock peach “Rubira?” shows a strong induced antixenosis-type GPA resistance.
This was demonstrated segregating as a single dominant gene. In order to investigate the genetic basis of resistance and develop
molecular tools useful in breeding programs, a F2 population derived from “Rubira?” also segregating for leaf color was grown and scored for GPA resistance under contrasted
environmental conditions. An SSR-based genetic linkage map composed of 120 SSR loci spanned over a distance of 497.8 cM was
then established. The GPA resistance mapped to a single locus at the bottom end of linkage group 1. We propose to name Rm2 the dominant allele of the underlying gene. Additionally, a reciprocal translocation was identified near the Gr gene controlling leaf color. The red-leaf parent “Rubira?” was demonstrated responsible for the translocation. This study
provides the basis for future molecular analysis for the use of Rm2 in peach breeding programs against GPA in peach orchards. 相似文献
14.
15.
Dadkhodaie NA Karaoglou H Wellings CR Park RF 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):479-487
The rust resistance genes Lr53 and Yr35, transferred to common wheat from Triticum dicoccoides, were reported previously to be completely linked on chromosome 6B. Four F
3 families were produced from a cross between a line carrying Lr53 and Yr35 (98M71) and the leaf rust and stripe rust susceptible genotype Avocet “S” and were rust tested using Puccinina triticina pathotype 53-1,(6),(7),10,11 and Puccinia striiformis f. sp. tritici pathotype 110 E143 A+. The homozygous resistant lines produced infection types of “;1−” and “;N” to these pathotypes, respectively.
The Chi-squared tests indicated goodness-of-fit of the data for one leaf rust gene and one stripe rust gene segregation. Linkage
analysis using this population demonstrated recombination of 3% between the genes. Microsatellite markers located on the short
arm of chromosome 6B were used to map the genes, with the markers cfd1 and gwm508 being mapped approximately 1.1 and 4.5 cM, respectively, proximal to Lr53. Additional studies of the relationship between Lr36, also located on the short arm of chromosome 6B, and Lr53 indicated that the two genes were independent. 相似文献
16.
Salt tolerance in Lycopersicon spp. VII. Pleiotropic action of genes controlling earliness on fruit yield 总被引:1,自引:0,他引:1
A. J. Monforte M. J. Asíns E. A. Carbonell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(3-4):593-601
The change from vegetative to reproductive development (earliness) in Lycopersicon chesmannii line L2 was delayed for 20 weeks when compared to other Lycopersicon species under greenhouse conditions. The interspecific hybrid of L. chesmannii L2 and L. esculentum E9, a cherry tomato cultivar, also showed this delay in reproductive development. The distribution of this character in the
F2-derived population showed a bimodal shape, plants could be scored easily as “early” or “late” in two nutrient conditions
(optimum and high salinity). A QTL with major effects on earliness was detected in salinity, which explained 35.6% of the
phenotypic variation. The effect of this QTL greatly diminished under control conditions, indicating differences in the genetic
control of earliness between treatments. ACC synthase or phytochrome B2 are the products of candidate genes for such a major
QTL. Other QTLs with minor effects, and epistatic interactions, are also involved in earliness under both conditions. A “late”
F2 subpopulation yielded twice as much as an “early” F2; conversely, “early” plants were taller than “late” plants, regardless of the treatment. QTL analysis, carried out in both
subpopulations, showed that yield differences may be explained by chesmannii alleles showing negative additive effects at some QTLs only in the “early” subpopulation. The effect of population subdivision
on QTL analysis was investigated by computer simulations to show sample-size or random effects; thus, important pleiotropic
or regulatory effects of genes controlling earliness on yield that affect QTL analysis, have been reveiled. Therefore alleles
controlling earliness in L. chesmannii have to be taken into account for a more efficient utilization of the genetic resources of this species.
Received: 30 June 1998 / Accepted: 31 August 1998 相似文献
17.
Fukino N Ohara T Monforte AJ Sugiyama M Sakata Y Kunihisa M Matsumoto S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,118(1):165-175
Powdery mildew caused by Podosphaera xanthii is an important foliar disease in melon. To find molecular markers for marker-assisted selection, we constructed a genetic
linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant AR
5 and susceptible ‘Earl’s Favourite (Harukei 3)’. The map spans 877 cM and consists of 167 markers, comprising 157 simple
sequence repeats (SSRs), 7 sequence characterized amplified region/cleavage amplified polymorphic sequence markers and 3 phenotypic
markers segregating into 20 linkage groups. Among them, 37 SSRs and 6 other markers were common to previous maps. Quantitative
trait locus (QTL) analysis identified two loci for resistance to powdery mildew. The effects of these QTLs varied depending
on strain and plant stage. The percentage of phenotypic variance explained for resistance to the pxA strain was similar between
QTLs (R
2 = 22–28%). For resistance to pxB strain, the QTL on linkage group (LG) XII was responsible for much more of the variance
(41–46%) than that on LG IIA (12–13%). The QTL on LG IIA was located between two SSR markers. Using an independent population,
we demonstrated the effectiveness of these markers. This is the first report of universal and effective markers linked to
a gene for powdery mildew resistance in melon. 相似文献
18.
A "defeated" rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv. oryzae 总被引:7,自引:0,他引:7
Li ZK Luo LJ Mei HW Paterson AH Zhao XH Zhong DB Wang YP Yu XQ Zhu L Tabien R Stansel JW Ying CS 《Molecular & general genetics : MGG》1999,261(1):58-63
The genetic components responsible for qualitative and quantitative resistance of rice plants to three strains (CR4, CXO8,
and CR6) of Xanthomonas oryzae pv. oryzae (Xoo) were investigated using a set of 315 recombinant inbred lines (RILs) from the cross Lemont (japonica) × Teqing (indica) and a complete linkage map with 182 well distributed RFLP markers. We mapped a major gene (Xa4) and ten quantitative trait loci (QTLs) which were largely responsible for segregation of the resistance phenotype in the
RILs. The Teqing allele at the Xa4 locus, Xa4
T
, acted as a dominant resistance gene against CR4 and CXO8. The breakdown of Xa4
T
-associated resistance mediated by the mutant allele at the avrXa4 locus in the virulent strain CR6 results from significant changes in both gene action (lose of dominance) and the magnitude
of gene effect (≈50% reduction). Nevertheless, Xa4
T
still acted as a recessive QTL with a significant residual effect against CR6. The mutant alleles at the avrXa4 locus in CXO8 and CR6 that lead to a reduction in effect, or “breakdown”, of Xa4
T
were apparently accompanied by corresponding penalties for their fitness. The quantitative component of resistance to Xoo in the RILs was largely due to a number of resistance QTLs. Most resistance QTLs mapped to genomic locations where major
resistance genes and/or QTLs for resistance to Xoo, blast and sheath blight were identified in the same cross. Most QTLs showed consistent levels of resistance against all
three Xoo strains. Our results suggest that a high level of durable resistance to Xoo may be achieved by the cumulative effects of multiple QTLs, including the residual effects of “defeated” major resistance
genes.
Received: 28 April 1998 / Accepted: 9 October 1998 相似文献
19.
Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize 总被引:1,自引:0,他引:1
Chung CL Poland J Kump K Benson J Longfellow J Walsh E Balint-Kurti P Nelson R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(2):307-326
To capture diverse alleles at a set of loci associated with disease resistance in maize, heterogeneous inbred family (HIF)
analysis was applied for targeted QTL mapping and near-isogenic line (NIL) development. Tropical maize lines CML52 and DK888
were chosen as donors of alleles based on their known resistance to multiple diseases. Chromosomal regions (“bins”; n = 39) associated with multiple disease resistance (MDR) were targeted based on a consensus map of disease QTLs in maize.
We generated HIFs segregating for the targeted loci but isogenic at ~97% of the genome. To test the hypothesis that CML52
and DK888 alleles at MDR hotspots condition broad-spectrum resistance, HIFs and derived NILs were tested for resistance to
northern leaf blight (NLB), southern leaf blight (SLB), gray leaf spot (GLS), anthracnose leaf blight (ALB), anthracnose stalk
rot (ASR), common rust, common smut, and Stewart’s wilt. Four NLB QTLs, two ASR QTLs, and one Stewart’s wilt QTL were identified.
In parallel, a population of 196 recombinant inbred lines (RILs) derived from B73 × CML52 was evaluated for resistance to
NLB, GLS, SLB, and ASR. The QTLs mapped (four for NLB, five for SLB, two for GLS, and two for ASR) mostly corresponded to
those found using the NILs. Combining HIF- and RIL-based analyses, we discovered two disease QTLs at which CML52 alleles were
favorable for more than one disease. A QTL in bin 1.06–1.07 conferred resistance to NLB and Stewart’s wilt, and a QTL in 6.05
conferred resistance to NLB and ASR. 相似文献
20.
Gao M Li G Yang B Qiu D Farnham M Quiros C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(2):277-287
We constructed a 1,257-marker, high-density genetic map of Brassica oleracea spanning 703 cM in nine linkage groups, designated LG1–LG9. It was developed in an F2 segregating population of 143 individuals
obtained by crossing double haploid plants of broccoli “Early-Big” and cauliflower “An-Nan Early”. These markers are randomly
distributed throughout the map, which includes a total of 1,062 genomic SRAP markers, 155 cDNA SRAP markers, 26 SSR markers,
3 broccoli BAC end sequences and 11 known Brassica genes: BoGSL-ALK, BoGSL-ELONG, BoGSL-PROa, BoGSL-PROb, BoCS-lyase, BoGS-OH, BoCYP79F1, BoS-GT (glucosinolate pathway), BoDM1 (resistance to downy mildew), BoCALa, BoAP1a (inflorescence architecture). BoDM1 and BoGSL-ELONG are linked on LG 2 at 0.8 cM, making it possible to use the glucosinolate gene as a marker for the disease resistance gene.
By QTL analysis, we found three segments involved in curd formation in cauliflower. The map was aligned to the C genome linkage
groups and chromosomes of B. oleracea and B. napus, and anchored to the physical map of A. thaliana. This map adds over 1,000 new markers to Brassica molecular tools.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献