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Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants 总被引:3,自引:0,他引:3
Kareem A. Mosa Kundan Kumar Sudesh Chhikara Joseph Mcdermott Zijuan Liu Craig Musante Jason C. White Om Parkash Dhankher 《Transgenic research》2012,21(6):1265-1277
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Cell‐type‐ and tissue‐specific transcriptomes of the white spruce (Picea glauca) bark unmask fine‐scale spatial patterns of constitutive and induced conifer defense
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Jose M. Celedon Macaire M.S. Yuen Angela Chiang Hannah Henderson Karen E. Reid Jörg Bohlmann 《The Plant journal : for cell and molecular biology》2017,92(4):710-726
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Shailendra Singh Seung Hyun Kang Wonkyu Lee Ashok Mulchandani Wilfred Chen 《Biotechnology and bioengineering》2010,105(4):780-785
Phytochelatin (PC) is a naturally occurring peptide with high affinity towards arsenic (As). In this article, we demonstrated the systematic engineering of PC‐producing E. coli for As accumulation by addressing different bottlenecks in PC synthesis as well as As transport. Phytochelatin synthase from Schizosaccharomyces pombe (SpPCS) was expressed in E. coli resulting in 18 times higher As accumulation. PC production was further increased by co‐expressing a feedback desensitized γ‐glutamylcysteine synthetase (GshI*), resulting in 30‐fold higher PC levels and additional 2‐fold higher As accumulation. The significantly increased PC levels were exploited further by co‐expressing an arsenic transporter GlpF, leading to an additional 1.5‐fold higher As accumulation. These engineering steps were finally combined in an arsenic efflux deletion E. coli strain to achieve an arsenic accumulation level of 16.8 µmol/g DCW, a 80‐fold improvement when compared to a control strain not producing phytochelatins. Biotechnol. Bioeng. 2010. 105: 780–785. © 2009 Wiley Periodicals, Inc. 相似文献
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