共查询到20条相似文献,搜索用时 31 毫秒
1.
H. Zhang J. Jia M. D. Gale K. M. Devos 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):69-75
A comparative genetic map of Aegilops umbellulata with wheat was constructed using RFLP probes that detect homoeoloci previously mapped in hexaploid bread wheat. All seven
Ae. umbellulata chromosomes display one or more rearrangements relative to wheat. These structural changes are consistent with the sub-terminal
morphology of chromosomes 2 U, 3 U, 6 U and 7 U. Comparison of the chromosomal locations assigned by mapping and those obtained
by hybridization to wheat/Ae. umbellulata single chromosome addition lines verified the composition of the added Ae. umbellulata chromosomes and indicated that no further cytological rearrangements had taken place during the production of the alien-wheat
aneuploid lines. Relationships between Ae. umbellulata and wheat chromosomes were confirmed, based on homoeology of the centromeric regions, for 1 U, 2 U, 3 U, 5 U and 7 U. However,
homoeology of the centromeric regions of 4 U with wheat group-6 chromosomes and of 6 U with wheat group-4 chromosomes was
also confirmed, suggesting that a re-naming of these chromosomes may be pertinent. The consequences of the rearrangements
of the Ae. umbellulata genome relative to wheat for gene introgression are discussed.
Received: 10 July 1997 / Accepted: 19 September 1997 相似文献
2.
3.
Shinrin-yoku (forest-air bathing and walking) effectively decreases blood glucose levels in diabetic patients 总被引:6,自引:0,他引:6
Y. Ohtsuka Noriyuki Yabunaka Shigeru Takayama 《International journal of biometeorology》1998,41(3):125-127
The influence of ”shinrin-yoku” (forest-air bathing and walking) on blood glucose levels in diabetic patients was examined.
Eighty-seven (29 male and 58 female) non-insulin-dependent diabetic patients [61 (SEM 1) years old] participated in the present
study. Shinrin-yoku was performed nine times over a period of 6 years. The patients were divided into two parties. They then
walked in the forest for 3 km or 6 km according to their physical ability and/or the existence of diabetic complications.
The mean blood glucose level after forest walking changed from 179 (SEM 4) mg · 100 ml–1 to 108 (SEM 2) mg · 100 ml–1 (P<0.0001). The level of glycated haemoglobin A1c also decreased from 6.9 (SEM 0.2)% (before the first shinrin-yoku) to 6.5 (SEM 0.1)% (after the last shinrin-yoku; P<0.05). Blood glucose values declined by 74 (SEM 9) mg · 100 ml–1 and 70 (SEM 4) mg · 100 ml–1 after short- and long-distance walking respectively. There was no significant difference between these values. Since the
forest environment causes changes in hormonal secretion and autonomic nervous functions, it is presumed that, in addition
to the increased calorie consumption and improved insulin sensitivity, walking in a forest environment has other beneficial
effects in decreasing blood glucose levels.
Received: 9 July 1997/Accepted: 20 October 1997 相似文献
4.
Y. Hisatomi K. Hanada S. Iida 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(7):1049-1056
Transposable elements have often been discovered as new insertion sequences in known genes, and minisatellites are often
employed as molecular markers in diagnostic and mapping studies. We compared the genes for flower pigmentation in a line of
the common morning glory bearing fully colored flowers with those in two anthocyanin
flaked
mutable lines producing variegated flowers and found RFLPs at the region of the ANS gene for anthocyanin biosynthesis. The DNA rearrangements detected by the RFLPs are due to integration of a novel type of
minisatellite, MiniSip1, having a long LTR retrotransposon, RTip1, inserted in the mutable lines. The structural analysis of the rearranged region revealed that the 12.4-kb RTip1 element is flanked by 5-bp target duplications within the MiniSip1 sequence and contains two LTR sequences of about 590 bp, a primer binding site for tRNALys, a typical polypurine tract and another new type of minisatellite, MiniSip2. Since no long open reading frame corresponding to the gag and pol genes was found, RTip1 appears to be a defective Ty3/gypsy-like element. Interestingly, the 269-bp-long MiniSip1 element comprises two alternating motifs of 41 bp and 19 bp, whereas the 962 bp long MiniSip2 element consists of two partially alternating motifs of 86 bp and 90 bp which are partially homologous to each other. Possible
evolutionary processes that may have generated the rearranged structure at the ANS gene region are also discussed.
Received: 25 April 1997 / Accepted: 16 May 1997 相似文献
5.
The objective of this study was to assess fermentation product, growth rate and growth yield responses of Selenomonas ruminantium HD4 to limiting and non-limiting ammonia concentrations. The ammonia half-inhibition constant for S. ruminantium in batch culture was 296 mM. Cells were grown in continuous culture with a defined ascorbate-reduced basal medium containing
either 0.5, 5, 25, 50, 100 or 200 mM NH4Cl and dilution rates were 0.07, 0.14, 0.24 or 0.40 h-1. Ammonia was the growth-limiting nutrient when 0.5 mM NH4Cl was provided and the half-saturation constant was 72 μM. Specific rates of glucose utilization and fermentation acid carbon
formation were highest for 0.5 mM NH4Cl. Lactate production (moles per mole of glucose disappearing) increased at the fastest dilution rate (0.40 h-1) for 5.0 mM NH4Cl while acetate and propionate decreased when compared to slower dilutions (0.07 and 0.14 h-1). Lactate production remained low while acetate and propionate remained high for all dilution rates when NH4Cl concentrations were 25 mM or greater. Yield (Y
Glc and Y
ATP) were nearly doubled when NH4Cl was increased from 0.5 mM (25.1 g cells/mol glucose used and 13.9 g cells/mol ATP produced respectively) to the higher
concentrations. Y
Glc was highest at 25 mM and 50 mM NH4Cl (48.2 cells/mol and 43.1 cells/mol respectively) as was Y
ATP (23.2 cells/mol and 20.8 cells/mol respectively). Y
NH3 was highest at the lowest NH4Cl concentration. The maximal fermentation product formation rate occurred at a growth-limiting ammonia concentration, while
maximal glucose and ATP bacterial yields occurred at non-growth-limiting ammonia concentrations. Given the growth response
of this ruminal bacterium, it is possible that maximization of ruminal bacterial yield may necessitate sacrificing the substrate
degradation rate and vice versa.
Received: 5 December 1995/Received revision: 2 April 1996/Accepted: 22 April 1996 相似文献
6.
Connie M. Williams Guichang Zhang Marek Michalak D. D. Cass 《Sexual plant reproduction》1997,10(2):83-88
To examine possible calcium (Ca2+)-mediated prefertilization events in male gametes of higher plants, we studied protein phosphorylation and the Ca2+-binding proteins, calmodulin and calreticulin, in sperm cells isolated from maize (Zea mays L.) pollen in the presence and absence of Ca2+. Using immunoblotting, we detected calmodulin and calreticulin and Ca2+-induced variations. Exposure of sperm cells to 1 mM Ca2+ for 1 h increased calmodulin content by 136% compared with the control. Ca2+ had little effect on calreticulin at 1 h, but induced a 34% increase after 3 h. Phosphorylation of proteins was low in 1 h-control
and Ca2+-treated cells. However, a 13-fold increase in phosphorylation of a 18-kDa protein was found at 12 h in the presence of Ca2+. Ca2+-induced changes in calmodulin, calreticulin and protein phosphorylation observed in maize sperm cells may reflect prefertilization
changes in vivo that facilitate sperm cell fusion with egg and central cells.
Received: 26 July 1996 / Revision accepted: 7 February 1997 相似文献
7.
The START cell cycle transition in the budding yeast Saccharomyces cerevisiae is catalyzed by the Cdc28 cyclin-dependent kinase associated with Cln-type cyclins. Since ectopic expression of the B-type
cyclin CLB5 can efficiently rescue the inviability that results from CLN depletion, we tested the specificity of the CLN and CLB classes of cyclins for promoting START-associated events. Several aspects of the regulation of the mating factor response
were compared for cells in which START activity was provided by either Cln-cyclins or Clb5. Unlike Cln1 and Cln2, high level
expression of Clb5 was unable to repress the activity of the mating factor response pathway at START. Downregulation of Far1
protein at START is normal in cln−GAL1::CLB5 cells. Even though the Clb5-Cdc28 kinase activity in cln−GAL1::CLB5 cells is not downregulated in response to mating factor, cells arrest in the first cycle after addition of mating factor
with a similar sensitivity as wild-type cells. However, whereas wild-type cells treated with mating factor arrest specifically
in G1 phase as unbudded cells with unreplicated DNA (pre-START), most cln−GAL1::CLB5 cells arrest as budded post-START cells with replicated DNA. Our findings demonstrate the ability of post-START cells to
arrest in response to mating factor and provide novel evidence for mechanisms that contribute to restrict mating factor-induced
arrest in wild-type cells to the G1 phase of the cell cycle.
Received: 25 September 1997 / Accepted: 9 November 1997 相似文献
8.
P. L. Pfahler M. J. Pereira R. D. Barnett 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1218-1222
In vitro pollen germination and tube length studies are valuable in elucidating mechanisms (germination capacity and rate, tube growth
rate) possibly associated with genetic differences in male transmission. On each of two collection dates, the percentage germination
and tube length of the binucleate pollen grains from five diverse sesame (Sesamum indicum L.) genotypes were determined at eight times (30, 60, 90, 120, 150, 180, 240, 300 min) after inoculation on a semisolid medium
containing 10% (100 g l-1) sucrose (C12H22O11), 0.4% (4 g l-1) purified agar (Fisher Lot 914409), 0.1% (1 g l-1) calcium nitrate [Ca(NO3)2 ⋅ 4H2O] and 0.01% (100 mg l-1) boric acid (H3BO3). Before heating, the pH of the medium was adjusted to 7.0 with a 0.1 N potassium hydroxide (KOH) solution. Over the five genotypes, 5% germination was found 30 min after inoculation and a maximum
of 37% germination 120 min after inoculation with no significant changes thereafter. As indicated by the highly significant
genotype×time after inoculation interaction, the genotypes differed in the time at which germination was initiated and maximum
germination attained. Over all five genotypes, the tube length was 91 μm 30 min after inoculation, reaching a maximum of 1000 μm
300 min after inoculation. As shown by the highly significant genotype×time after inoculation interaction, the genotypes differed
in the time at which tube length was observed and the maximum tube length was attained. Little or no relationship between
percent germination and tube length was observed among the genotypes. For both percent germination and tube length, the statistical
significance of collection date and its interactions with genotype and time after inoculation indicated that environment in
the form of collection date was also an influencing factor. These results indicated that genetic differences among genotypes
were present for in vitro germination capacity, germination rate and tube growth rate and that these factors singly or in combination could alter male
transmission of genetic elements.
Received: 5 February 1997 / Accepted: 23 June 1997 相似文献
9.
Construction of an RFLP linkage map for cultivated sunflower 总被引:5,自引:0,他引:5
C. C. Jan B. A. Vick J. F. Miller A. L. Kahler E. T. Butler III. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):15-22
An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus
a fertility restoration gene, Rf
1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation,
with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters
of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with
11 markerless regions exceeding 20 cM.
Received: 17 June 1997 / Accepted: 19 June 1997 相似文献
10.
The New Zealand hagfish, Eptatretus cirrhatus, is known to eliminate parts of its chromosomes during embryogenesis from presumptive somatic cells. Electrophoresis of germ
line and somatic DNAs of this species, after treatment with the restriction endonucleases DraI and EcoRI, revealed three fragments
of DNA that were restricted to the germ line. DNA filter hybridization experiments demonstrated that these fragments were
present almost exclusively in the germ line DNA of E. cirrhatus and that they were highly and tandemly repeated. Thus, these DNA fragments appeared to be eliminated during embryogenesis.
Moreover, one fragment (a DraI fragment) cross-hybridized with the germ line DNA from other species of hagfish, namely, Eptatretus okinoseanus and Paramyxine atami. Molecular cloning and sequence analysis revealed that the DraI fragment was composed mainly of closely related sequences
of 85 bp in length and that this sequence was about 75% homologous to the sequence of EEEo2 (eliminated element of E. okinoseanus 2) which is a germ line-restricted and highly repetitive sequence that was isolated previously from E. okinoseanus. The other two fragments were composed of three families of closely related sequences that were 172 bp long (designated EEEc1),
61 bp long (EEEc2) and 54 bp long (EEEc3). Fluorescence in situ hybridization experiments revealed that each eliminated element
was distributed on several chromosomes that are limited to germ cells. EEEo2 was dispersed on 12 C-band-positive chromosomes.
EEEc1 and EEEc3 were dispersed on all C-band-positive and several C-band-negative chromosomes. By contrast, EEEc2 was located
to terminal regions of several C-band-negative chromosomes. These results suggest that the eliminated chromosomes in hagfish
are mosaics of highly repeated, germ line-restricted families of DNA sequences.
Received: ██; in revised form: 25 October 1997 / Accepted: ██ 相似文献
11.
Tropical zooplankton in the highly-enclosed lagoon of Taiaro Atoll (Tuamotu Archipelago, French Polynesia) 总被引:1,自引:0,他引:1
Nocturnal zooplankton assemblages around Taiaro Atoll were sampled over six nights during February 1994. Replicate zooplankton
samples were collected at windward and leeward locations in the enclosed lagoon and adjacent ocean with a metered net (85 cm
diameter, 500 μm mesh) towed for 15 min at 5 m depth. The zooplankton community in the lagoon was very different from that
in the ocean. Oceanic samples contained 50 mostly holoplanktonic taxa (diversity index, H′=2.62; evenness index, J′=0.67).
Lagoonal samples contained 19 mostly meroplanktonic taxa (H′=1.54, J′=0.52) with three taxa (decapod larvae; an ostracod,
Cypridina sp.; a copepod, Acartia fossae) contributing >90% of the individuals. Unlike the ocean, zooplankton distributions in the lagoon were not homogenous; instead
spatial patterns were apparently formed by the interaction between hydrodynamic processes and species-specific behaviour.
Accepted: 28 August 1997 相似文献
12.
Andreas Houben Carolyn R. Leach Dawn Verlin Ruth Rofe Jeremy N. Timmis 《Chromosoma》1997,106(8):513-519
Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from
plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere,
rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After
in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could
be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome.
Received: 30 May 1997; in revised form: 20 August 1997 / Accepted: 20 August 1997 相似文献
13.
Trisomy associated with loss of maturation capacity in a long-term embryogenic culture of Abies alba
R. Roth I. Ebert J. Schmidt 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(3):353-358
Karyological studies were made on a 6-year-old embryogenic cell line of Abies alba. Embryogenic cells were obtained from a mature zygotic embryo cultivated on modified MCM-medium and subcultured every 3 weeks.
Three years after induction, part of the cell line was transferred to media supplemented with 500 or 1000 mg l-1 caseine hydrolysate and 500 mg l-1
L-glutamine. Approximately 3 years after addition of the organic nitrogen to the medium, morphological changes such as malformation
of the suspensor cells and a loss of maturation capacity occurred. Chromosome counts revealed that all cells cultivated on
medium with organic nitrogen were trisomic. Fluorescent-banding methods and comparison with an euploid cell line showed that
the additional chromosome belonged to the group of long, metacentric chromosomes of Abies alba without secondary constriction. Those cells cultured on medium not supplemented with caseine hydrolysate and L-glutamine retained a stable chromosome number of 2n=24. Both normal and deformed suspensor cells were observed. The maturation
frequency was very low. The emergence of aneuploidy within one cell line could be the consequence of high selection pressure
caused by the different culture conditions.
Received: 27 February 1997 / Accepted: 7 March 1997 相似文献
14.
S. C. K. Milach H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):783-790
Restriction fragment length polymorphism (RFLP) analysis provides a valuable tool for characterizing and understanding relationships
among genes for useful traits in crop species, particularly in ones with complex genomes such as the hexaploid cultivated
oat Avena sativa L. (2n=6x=42). Using Bulked Segregant Analysis (BSA) and F2 RFLP linkage data, we mapped three dominant oat dwarfing loci to different regions of the oat genome. Dw6, in oat line OT207, is 3.3±1.3 cM from the Xumn145B locus, which has not been placed on the hexaploid oat linkage map. Dw7, in line NC2469-3, is 4.3±2.3 cM from Xcdo1437B and 33±4.1 cM from Xcdo708B. This places Dw7 to linkage group 22. Dw8, in the Japanese lines AV17/3/10 and AV18/2/4, mapped 4.9±2.2 cM from Xcdo1319A in an AV17/3/10בKanota’ F2 population and 6.6±2.6 cM from it in an AV18/2/4בKanota’ population. This places Dw8 to linkage group 3. Aneuploid analysis of markers linked to the dwarfing genes located Dw6 on the smallest oat chromosome (chromosome 18) and Dw7 on the longest satellited chromosome (chromosome 19). The RFLP markers closely linked to the three dwarfing genes identify
distinct regions of the oat genome that contribute to plant height and they should be useful in characterizing new genetic
sources of dwarfness in oat.
Received: 8 May 1997 / Accepted: 20 May 1997 相似文献
15.
When cultured together in a primary serum-free hormone-free system, hepatocytes and exocrine pancreas cells from the carp,
Cyprinus carpio, spontaneously establish unique morphological structures that do not occur in vivo. These structures include intercellular
bile canaliculi between neighbouring hepatocytes and hybrid canaliculi between hepatocytes and pancreas cells. In vivo, carp
hepatocytes form only unicellular bile canaliculi; hybrid canaliculi between hepatocytes and exocrine pancreas cells do not
exist at all in nature. This study shows that, in an artificial environment, cells are able spontaneously to establish novel
morphological structures that are absent in the animal from which the cells have been obtained.
Received: 3 January 1996 / Accepted: 17 March 1997 相似文献
16.
G. R. V. Babu O. K. Vijaya V. L. Ross J. H. Wolfram K. D. Chapatwala 《Applied microbiology and biotechnology》1996,45(1-2):273-277
Our isolate, Pseudomonas putida, is known to be capable of utilizing cyanides as the sole source of carbon (C) and nitrogen (N) both in the form of free cells
and cells immobilized in calcium alginate. In the present study, the cell-free extract(s) were prepared from the cells of
P. putida grown in the presence of sodium cyanide. The ability of enzyme(s) to convert cyanides, cyanates, thiocyanates, formamide and
cyanide-containing mine waters into ammonia (NH3) was studied at pH 7.5 and pH 9.5. The kinetic analysis of cyanide and formamide conversion into NH3 at pH 7.5 and pH 9.5 by the cell-free extract(s) of P. putida was also studied. The K
m and V
max values for cyanide/formamide were found to be 4.3/8 mM and 142/227 μmol NH3 released mg protein-1 min-1 respectively at pH 7.5 and 5/16.67 mM and 181/434 μmol NH3 released mg protein-1 h-1 respectively at pH 9.5. The study thus concludes that the cell-free extract(s) of P. putida is able to metabolize not only cyanides, cyanates, thiocyanates, and formamide but also cyanide-containing mine waters to
NH3.
Received: 10 April 1995/Received revision: 24 July 1995/Accepted: 22 August 1995 相似文献
17.
R. N. Morgan J. P. Wilson W. W. Hanna P. Ozias-Akins 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):413-420
Pearl millet [Pennisetum glaucum (L.) R.Br.] is a warm-season grass used for food, feed, fodder and forage, primarily in countries of Africa and India but
grown around the world. The two most-destructive diseases to pearl millet in the United States are rust (caused by Puccinia substriata var. indica) and pyricularia leaf spot (caused by Pyricularia grisea). Genes for disease resistance to both pathogens have been transferred into agronomically acceptable forage and grain cultivars.
A study was undertaken to identify molecular markers for three rust loci and one pyricularia resistance locus. Three segregating
populations were screened for RAPDs using random decamer primers and for RFLPs using a core set of probes detecting single-copy
markers on the pearl millet map. The rust resistance gene Rr
1
from the pearl millet subspecies P. glaucum ssp. monodii was linked 8.5 cM from the RAPD OP-G8350. The linkage of two RFLP markers, Xpsm108 (15.5 cM) and Xpsm174 (17.7 cM), placed the Rr
1
gene on linkage-group 3 of the pearl millet map. Rust resistance genes from both Tift 89D2 and ICMP 83506 were placed on linkage-group 4 by determining genetic linkage to the RFLP marker Xpsm716 (4.9 and 0.0 cM, respectively). Resistance in ICMP 83506 was also linked to the RFLP marker Xpsm306 (10.0 cM), while resistance in Tift 89D2 was linked to RAPD markers OP-K19350 (8.8 cM) and OP-O8350 (19.6 cM). Fragments from OP-K19 and OP-O8 in the ICMP 83506 population, and Xpsm306 in the Tift 89D2 population, were monomorphic. Only one RAPD marker (OP-D11700, 5.6 cM) was linked to pyricularia leaf spot resistance. Attempts to detect polymorphisms with rice RFLP probes linked to
rice blast resistance (Pyricularia oryzae; syn=P. grisea) were unsuccessful.
Received: 19 May 1997 / Accepted: 21 October 1997 相似文献
18.
Establishment of stable NaCl-resistant rice plant lines from anther culture: distribution pattern of K+/Na+ in callus and plant cells 总被引:1,自引:0,他引:1
P. Sathish O. L. Gamborg M. W. Nabors 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1203-1209
Soil salinity markedly suppresses the growth of rice (Oryza sativa L.). We established rice anther culture to select for rice callus lines adapted to NaCl stress and regenerated plant progenies
resistant to a NaCl stress of E.C. 16–18 mS. When exposed to NaCl, NaCl-adapted rice calli lost K+ and accumulated little Na+. Conversely, plant cells lost relatively little K+ and accumulated Na+. It is plausible that, NaCl-resistant mechanisms are different at callus and plant level. The stable NaCl-resistant lines
produced have potential use in elucidating the molecular mechanisms behind NaCl resistance in rice and in rice breeding.
Received: 27 February 1997 / Accepted: 4 April 1997 相似文献
19.
T. Matsunaga H. Sudo H. Takemasa Y. Wachi N. Nakamura 《Applied microbiology and biotechnology》1996,45(1-2):24-27
The cyanobacterium, Aphanocapsa halo-phytia MN-11, was immobilized in calcium alginate gel and coated on light-diffusing optical fibers (LDOF) for sulfated extracellular
polysaccharide production. Results indicated that sulfated extracellular polysaccharide production depends on the number of
immobilized cells and the light intensity. In addition, the production rate reached 116.0 mg (mg dry cells)-1 day-1 when the cells that were immobilized on LDOF were incubated under a light intensity of 1380 cd sr m-2 at a cell concentration of 1.0×108 cells/cm3 gel. Cells immobilized on LDOF produced about ten times more sulfated extracellular polysaccharide than those immobilized
in calcium alginate beads only (11.7 mg(mg dry cells)-1 day-1).
Received: 31 March 1995/Revised last revision 12 June 1995/Accepted 26 July 1995 相似文献
20.
Locating the petunia Rf gene on a 650-kb DNA fragment 总被引:1,自引:0,他引:1
S. Bentolila J. Zethof T. Gerats M. R. Hanson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):980-988
A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf ) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf (<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement between the petunia and the tomato genomes, this
synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf. Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb MluI fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.
Received: 20 August 1997 / Accepted: 21 October 1997 相似文献