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1.
Implanting ovaries or injecting 20-hydroxyecdysone into male houseflies induced sex pheromone production, including (Z)-9-tricosene (muscalure), 9,10-epoxytricosane and (Z)-14-tricosen-10-one, which normally occurs only in vitellogenic females. Control males did not produce detectable amounts of these compounds. Injection of 20-hydroxyecdysone (5 μg/insect per day) for 3 days resulted in the accumulation of 1.81 μg/insect of (Z)-9-tricosene, 0.97 μg/insect of 9,10-epoxytricosane and 0.12 μg/insect (Z)-14-tricosen-10-one. Multiple injections of 20-hydroxyecdysone at doses as low as 50 ng resulted in the accumulation of 23:1, C23 epoxide and C23 ketone; shifted the distribution of label within the alkenes from 27:1 to 23:1 and decreased the amount of label in the hydrocarbon fractions as alkenes. Structures of the C23 alkene and epoxide produced by the males were verified by gas chromatography-mass spectrometry. Radioactivity from [1-14C] acetate was incorporated into the C23 alkene, epoxide and ketone in male insects after ovaries were implanted or they were injected with 20-hydroxyecdysone. Synthesis of the C23 pheromone components decreased rapidly within several days after the administration of 20-hydroxyecdysone ceased, indicating that the enzymes involved in sex pheromone production were not permanently induced by hormone treatment. Ecdysone was also effective in initianing pheromone production in males, whereas inokosterone and cholesterol were not effective. Data presented demonstrate that male houseflies possess the metabolic capability to produce the sex pheromone components, and this suggests that 20-hydroxyecdysone alters the production of cuticular hydrocarbons such that the C23 sex pheromone components become major products. 相似文献
2.
《Insect Biochemistry》1984,14(3):279-284
Components of the sex pheromone of the female housefly, (Z)-9,10-epoxytricosane and (Z)-14-tricosen-10-one, are absent on the surface of newly emerged insects, first appear on females on day 2, and increase in amount to day 10. All body parts contain these components, with the legs and abdomen containing the largest amounts. The incorporation of [1-14C]acetate into the non-hydrocarbon cuticular (NHC) fraction, which includes 9,10-epoxytricosane and (Z)-14-tricosen-10-one is very low in newly emerged and one-day-old female houseflies and then increases dramatically from day 2 to 6. The major labelled components in this fraction are the C23 epoxide and ketone. The increased amounts and incorporation of [1-14C]acetate into the C23 epoxide and ketone correlate closely with the production of (Z)-9-tricosene. [9,10-3H](Z)-9-Tricosene is readily converted to oxygenated components in female insects giving rise to the C23 epoxide (85.5%), C23 ketone (13.0%) and more polar components (1.5%). Both female and male insects of all ages metabolize [9,10-3H](Z)-9-tricosene to the epoxide and ketone. All major body parts in both males and females metabolized (Z)-9-tricosene when it was applied to the surface of the insect, with the highest rate of metabolism observed by the legs of male insects. 相似文献
3.
Sami Ahmad Kenneth E. Kirkland Gary J. Blomquist 《Archives of insect biochemistry and physiology》1987,6(2):121-140
Direct evidence is presented for the role of a cytochrome P-450 monooxygenase (called mixed-function oxidase, or polysubstrate mono-oxygenase, PSMO) in the metabolism of the sex pheromone (Z)-9-tricosene to its corresponding epoxide and ketone in the housefly. A secondary alcohol, most likely an intermediate in the conversion of the alkene to the ketone, was also tentatively identified. The results of in vivo and in vitro experiments showed that the PSMO inhibitors, piperonyl butoxide (PB) and carbon monoxide, markedly inhibited the formation of epoxide and ketone from (9,10-3H) (Z)-9-tricosene. An examination of the relative rates of (Z)-9-tricosene metabolism showed that males exhibited a higher rate of metabolism than females with the antennae of males showing the highest activity of any tissue/organ examined. The major product from all tissues/organs was the epoxide. Data from experiments with subcellular fractions showed that the microsomal fraction had the majority of enzyme activity, which was strongly inhibited by PB and CO and required NADPH and O2 for activity. A carbon monoxide difference spectrum with reduced cytochrome showed maximal absorbance at 450 nm and allowed quantification of the cytochrome P-450 in the microsomal fraction of 0.410-nmol cytochrome P-450 mg?1 protein. Interaction of (Z)-9-tricosene with the cytochrome P-450 resulted in a type I spectrum, indicating that the pheromone binds to a hydrophobic site adjacent to the heme moiety of the oxidized cytochrome P-450. 相似文献
4.
Houseflies ovariectomized within 12 h after emergence do not produce (Z)-9-tricosene nor demonstrate the shift from alkene to alkane synthesis that is typcal of flies with developing ovaries. A single injection of 20-hydroxyecdysone at doses of 0.1 to 10 μg will induce the pattern in ovariectomized insects that is characteristic of flies with ovaries. Furthermore, this pattern persists for 3 days, but by 6 days after hormone injection, the synthesis of (Z)-9-tricosene stops and more alkenes are produced than alkanes. A post-hormone treatment time of 16 h was required before detectable amounts of (Z)-9-tricosene appeared on ovariectomized flies. Multiple injections of 20-hydroxyecdysone at doses of 50 ng into ovariectomized flies induced (Z)-9-tricosene synthesis and a shift in alkene to alkane synthesis. Thus, 20-hydroxyecdysone was able to act as an ovarian substitute in ovariectomized flies by stimulating pheromone synthesis. 相似文献
5.
Effect of age and sex on the production of internal and external hydrocarbons and pheromones in the housefly, Musca domestica 总被引:2,自引:0,他引:2
Mpuru S Blomquist GJ Schal C Roux M Kuenzli M Dusticier G Clément JL Bagnères AG 《Insect biochemistry and molecular biology》2001,31(2):139-155
The epicuticular and internal waxes of male and female houseflies were examined by capillary gas chromatography-mass spectrometry at closely timed intervals from emergence until day-6 of adulthood. New components identified included tricosan-10-one, 9,10-epoxyheptacosane, heptacosen-12-one, a series of odd-carbon numbered dienes from C31 to C39, several positional isomers of monoenes including (Z)-9- and 7-pentacosene and a number of methyl- and dimethylalkanes. (Z)-9-tricosene appears in internal lipids prior to appearing on the surface of the insect, suggesting that it is transported in the hemolymph to its site of deposition on the epicuticle. The large increases in the amount of (Z)-9-tricosene in females from day-2 until day-6 is compensated for by a concomitant decrease in (Z)-9-heptacosene. The C23 epoxide and ketone only appear in females after the production of (Z)-9-tricosene is induced, and are only abundant in epicuticular waxes, suggesting they are formed after (Z)-9-tricosene is transported to the cells which are involved in taking them to the surface of the insect. Mathematical analysis indicated that the time shift between internal production and external accumulation in females is more than 24 h. The divergence between male and female lipid production occurs at an early stage, when insects are less than one day old. 相似文献
6.
Using gas chromatography the relative amounts of (Z)-9-tricosene (muscalure) and some other hydrocarbons on the cuticle of 1- to 20-day-old houseflies (Musca domestica L.) from different strains were determined. Flies from a WHO strain, in culture since 1961, and first-generation laboratory-cultured flies from two wild-type strains from a poultry breeding and a cow-house with pigsty, respectively, were compared. On WHO females hydrocarbons with 23–25 C atoms constituted about 65% of the total hydrocarbons, whereas on wild-type females less than 2% of these compounds was present. (Z)-9-tricosene comprised up to 20–30% of the total hydrocarbons on 5- to 20-day-old WHO females, whereas less than 0.5% (Z)-9-tricosene was present on the wild-type females. We also compared the amounts of (Z)-9-tricosene and some other hydrocarbons on female houseflies, kept in culture in the laboratory for several generations. It appeared that whereas on first-generation wild-type females hardly or no (Z)-9-tricosene could be detected, the amounts of this substance had increased considerably after some tens of generations in the laboratory. It is suggested that this was due to selection in subsequent generations of high-density populations. Production of (Z)-9-tricosene and of tricosane was shown to be closely linked. Selection did not affect the production of other cuticular hydrocarbons by the females. It is suggested that in mixed populations (both sexes together in a cage) in the course of time (Z)-9-tricosene is transferred from females to males and (Z)-9-heptacosene from males to females. It is concluded that reproductive ability of houseflies does not primarily depend on the amounts of (Z)-9-tricosene on females, although higher amounts of this substance may increase contacts between males and females. 相似文献
7.
Physiological amounts of 20-hydroxyecdysone do not initiate vitellogenin synthesis in unfed, non-vitellogenic mosquitoes. Injecting more than 10,000 times the physiological amount induced synthesis, but considerably less than was induced by a blood meal. A dose of 20-hydroxyecdysone which exceeded the physiological level only several hundred times, did not sustain vitellogenin synthesis, when blood-fed mosquitoes were ovariectomized just prior to injection. Transplanting ovaries from vitellogenic to non-vitellogenic females did not initiate synthesis of vitellogenin in the recipient. In vitro, neither 20-hydroxyecdysone nor the ovaries of vitellogenic females were able to induce synthesis of vitellogenin in non-vitellogenic fat bodies. These experiments suggest that ecdysteroid, released by the ovaries, does not initiate ovarian development in mosquitoes. 相似文献
8.
Chemical signals originating from the ovaries of gravid females of Musca domestica (Diptera: Cyclorrhapha: Muscidae) attract ovipositing females to common egg-laying sites. Behavioral experiments indicated that females preferred to oviposit in fermented wheat bran containing ovaries from reproductively mature houseflies. Females preferred to oviposit in fermented wheat bran than wet wheat bran. This effect was additive with the attraction to housefly ovaries. Solvent extracts from housefly ovaries were attractive to gravid females. Extracts obtained with hexane were most attractive to gravid females for egg laying, and extracts obtained with ethyl acetate attracted more egg laying than extracts obtained by dichloromethane. Gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC-MS) analysis showed that tricosane and (Z)-9-tricosene were the main components of the hexane extracts. Both tricosane and (Z)-9-tricosene were shown to elicit dose-dependent aggregation of gravid females in oviposition bioassays, but high doses of either chemical were not attractive. 相似文献
9.
In mosquitoes, a hormone (egg development neurosecretory hormone or EDNH), produced by the medial neurosecretory cells and stored in the corpus cardiacum soon after eclosion, is released after a blood meal, and vitellogenesis begins a few hours later. When either the ovaries or the neurosecretory cells and corpus cardiacum are removed before the blood meal, vitellogenin is not synthesized. Therefore, we tested the hypothesis that the release of EDNH from the corpus cardiacum is dependent on the secretion of a releasing factor from the ovaries.Using a bioassay for EDNH in the corpus cardiacum, we found that the gland of an ovariectomized female remained active after blood feeding, and therefore, has not released EDNH. When an ovary was implanted before the blood meal, the corpus cardiacum was inactive, and therefore, had released EDNH. We concluded that the ovaries secrete an EDNH-releasing factor, and that this factor and EDNH must both be in circulation before vitellogenesis can begin. Although releasing factor alone did not stimulate vitellogenesis, it was the rate limiting process that controlled the onset of vitellogenesis.Using a bioassay for the EDNH-releasing factor from the ovaries and using rocket-immuno-electrophoresis, we showed that a Culex ovary, but not an Anopheles ovary, could replaces Aedes ovaries as a source of the releasing factor.In Ae. aegypti, EDNH-releasing factor was required again after oviposition in order to reinitiate the vitellogenic process in females that took a second blood meal. Thus, the releasing factor is part of the mechanism regulating cyclic egg maturation in mosquitoes. 相似文献
10.
Sticky card captures of house flies, Musca domestica L. (Diptera: Muscidae), were used to compare efficacy of screen-covered baits containing sugar, sugar and 0.1% (Z)-9-tricosene, sugar and 1.0% (Z)-9-tricosene, Golden Malrin [1.1% methomyl and 0.049% (Z)-9-tricosene], and Quick-Bayt [0.5% imidacloprid and 0.1% (Z)-9-tricosene]. The QuickBayt treatment caught more flies per hour (mean = 116.5) than sugar alone (mean = 81.0), but the addition of (Z)-9-tricosene to sugar did not increase fly capture compared with sugar alone. More males (65% of total) than females were collected on the sticky cards for all treatments. Fly kill by plain sugar (control) and the commercial baits Golden Malrin, QuikStrike Fly Abatement strips (1.0% nithiazine), and QuickBayt was tested over a 90-min period. An average of 1.4, 5.6, 363.0, and 1,266.0 flies were killed using sugar, Golden Malrin, QuikStrike, and QuickBayt, respectively. The similarity between Golden Malrin and plain sugar reflects severe resistance to this once effective methomyl bait. A no-choice feeding assay using lab-reared methomyl-susceptible and methomyl-resistant house flies was conducted with and without (Z)-9-tricosene. Adult mortality was significantly higher in the methomyl-susceptible strain exposed to treatments containing methomyl. Lower consumption of the methomyl treatments by resistant flies suggested resistance was behavioral and mortality was not influenced by (Z)-9-tricosene for either fly strain. 相似文献
11.
《Insect Biochemistry》1978,8(3):149-154
Synthesis of vitellogenin and its release into the haemolymph of vitellogenic and ovariectomized females of Leucophaea maderae were monitored. Six-and-a-half months after ovariectomy, release of vitellogenin from the fat bodies had declined to about 12% of that in normal females. At this time, vitellogenin made up over 70% of the total haemolymph proteins in ovariectomized females, whereas in normal vitellogenic females it represented only 5–6%. Synthesis of vitellogenin on ergastoplasmic membranes of the fat bodies continued in ovariectomized females at nearly the same level as in unoperated ones. Most of this vitellogenin was stored in the fat bodies. From these data it is concluded that a high vitellogenin titre in the haemolymph inhibits its further release from the fat bodies, yet has no or very little effect on vitellogenin production. In this species the ovaries do not exert any control over vitellogenin synthesis. 相似文献
12.
13.
The number of adult male and female house flies, Musca domestica L. (Diptera: Muscidae), near varying levels of (Z)-9-tricosene alone (5, 50, or 100 micdrol) or combined (50 microl) with sugar was determined using conical screened traps on a dairy in southern California. Overall, significantly more males than females were collected in the traps. Significantly more flies (male and female) were collected in traps with (Z)-9-tricosene. There were no significant differences among doses of (Z)-9-tricosene alone, but numbers of both sexes were significantly higher in traps baited with (Z)-9-tricosene and sugar compared with the 5- and 50-microl doses without sugar. The age of female flies collected in traps was determined by pterin analysis. Mean female ages ranged from 94.7 to 99.6 degree-days (6.3-6.8 d of age) and did not differ significantly among treatments. Dissections of a subset of females from each treatment determined that collected females were primarily nongravid (86.3%). Proportions of gravid females that were collected did not differ among treatments. 相似文献
14.
Luckenbach JA Kusakabe M Swanson P Young G 《Journal of experimental zoology. Part A, Ecological genetics and physiology》2008,309(8):468-476
Unilateral ovariectomy (ULO, removal of one ovary) is a powerful technique for studying aspects of reproductive physiology, including follicular recruitment and growth. To examine effects of ULO for the first time in a semelparous species, coho salmon (Oncorhynchus kisutch) were unilaterally ovariectomized during mid-vitellogenesis approximately 3 months before spawning. At termination of the study (79 days post-surgery), single ovaries of ULO fish were gravimetrically equivalent to paired ovaries of sham surgery, control fish. There was no evidence of recruitment of new vitellogenic follicles. Instead, the dramatic increase in ovary mass was attributable to hypertrophy of existing vitellogenic follicles (33% increase in volume) and increased fecundity achieved through a greater than two-fold reduction in follicular atresia. The composition of whole ovaries on a dry weight basis from ULO fish was greater in protein, but lower in lipid than that of control fish. Expressing the data on a per follicle basis, however, showed that follicles of ULO fish contained more protein, ash, water, and lipid. The results indicate that ULO of coho salmon induces compensatory hypertrophy of existing vitellogenic follicles, while maximizing fecundity through reduction of atresia. Thus, 3 months before spawning, coho salmon exhibit the ability to adjust final egg size and number when faced with significant depletion of ovarian follicles. This in vivo system provides a platform for further study of physiological mechanisms regulating follicular growth and atresia, and the trade-off between egg size and egg number. J. Exp. Zool. 309A:468-476, 2008. (c) 2008 Wiley-Liss, Inc. 相似文献
15.
Klea Lamnissou 《Genesis (New York, N.Y. : 2000)》1997,20(1):47-52
In interspecific matings between Drosophila virilis and Drosophila texana female sterility is observed in F2 hybrid females. A previous study has shown that no vitellogenin synthesis occurs in the fat body of sterile hybrid females. The results presented in this paper show that hybrid ovaries of sterile females transplanted into the abdomens of females of the parental species are not able to develop upon maturity. With few exceptions, the hybrid ovaries remained alive in the host environment, but their oocytes failed to develop to vitellogenic stages. Thus, in hybrid females between Drosophila virilis and Drosophila texana sterility is the result of defects in both the two main developmental processes of egg maturation, the synthesis of vitellogenins in the fat body and the uptake of vitellogenins by the ovary. Dev Genet 20:47–52, 1997. © 1997 Wiley-Liss, Inc. 相似文献
16.
Quentin Fouche Damien Charabidze Christophe Lucas 《Medical and veterinary entomology》2023,37(1):170-175
Chemical signals are widespread in insects, but those resulting in interspecific communication (i.e., synomones) remain understudied. Here, we analysed chemicals left on substrates by two species of blow fly larvae, Lucilia sericata (Meigen) and Calliphora vomitoria (Linneaus) (Diptera: Calliphoridae), which can aggregate together on carrion. Using solid-phase microextraction and dynamic headspace analysis, we identified six compounds common to both species: the decanoic, tetradecanoic, pentadecanoic, hexadecanoic and octadecanoic acids, and the 2-ethylhexyl salicylate. We then tested the behavioural effects of the decanoic and pentadecanoic acids using binary-choice experiments, along with the (Z)-9-tricosene, a pheromone found in many arthropods. The time spent by a larva and its average crawling speed were measured in two sides of an arena, where only one contained a compound at 0.25 or 25 μg/μl. No effect was observed when testing the decanoic acid. The pentadecanoic acid only reduced the speed of C. vomitoria larvae at 25 μg/μl. Finally, L. sericata larvae spent less time in the side containing the (Z)-9-tricosene at 0.25 μg/μl, whereas C. vomitoria spent more time and crawled faster in this side at 25 μg/μl. Although these results did not directly evidence synomones, they suggest that the (Z)-9-tricosene could regulate larval aggregations on carrion. 相似文献
17.
B.P. Girish CH. Swetha P. Sreenivasula Reddy 《Biochemical and biophysical research communications》2014
The objective of the present study was to explore the site of synthesis of vitellogenin (Vtg) in fresh water edible crab, Oziothelphusa senex senex. Vtg cDNA fragments were isolated from the hepatopancreas of female crabs using RT-PCR method, and the deduced amino acid sequence of O. senex senex showed more than 60% identity with other brachyuran Vtg sequences. RT-PCR analysis showed that Vtg mRNA can be detected only in hepatopancreas of female Oziothelphusa but not in other tissues including eyestalks, Y-organs, mandibular organs, thoracic ganglion, hypodermis and ovary. Antibodies were raised against vitellin purified from the ovary of O. senex senex. Immunoprecipitation analysis revealed the presence of Vtg in the hepatopancreas of vitellogenic stage I females and in the hemolymph, hepatopancreas and ovary extracts from vitellogenic stage II females but absent in hemolymph and hepatopancreas extract of males. These results suggest that Vtg is synthesized only in hepatopancreas but not in the ovaries of O. senex senex. In addition, Vtg synthesized in hepatopancreas is transported to ovary through hemolymph. 相似文献
18.
七星瓢虫的卵黄发生:脂肪体与卵巢中卵黄原蛋白的合成与分泌 总被引:1,自引:1,他引:0
本文利用[3H]亮氨酸参入及特异性抗体沉淀等方法,研究了七星瓢虫体外培养的脂肪体中卵黄原蛋白合成与分泌的动力学,以及不同发育期脂肪体与卵巢中卵黄原蛋白合成的定量变化。脂肪体中卵黄原蛋白的合成与分泌在培养1—4小时内直线上升,到6小时稍下降。保留在脂肪体内的卵黄原蛋白缓慢积累,但一直水平很低。卵黄原蛋白合成的最初30分钟,分泌速率较慢,60%以上的卵黄原蛋白保留在脂肪体内。1小时后分泌速率加快,70%以上的卵黄原蛋白被分泌,保留的卵黄原蛋白在4小时中逐渐被释放。在4小时,被分泌的卵黄原蛋白超过80%,最高可达92%。 在雌虫发育过程中,脂肪体中卵黄原蛋白合成的高峰在羽化后11—15天,所合成的卵黄原蛋白占整个发育期合成总量的80%。在合成高峰期分泌的卵黄原蛋白高达90%以上,但在发育的早期和晚期分泌的卵黄原蛋白仅占30%或稍多。 卵黄发生前的卵巢就开始合成卵黄原蛋白,但卵巢中卵黄原蛋白的合成高峰期与脂肪体中大致相同。与脂肪体相反,卵巢合成的卵黄原蛋白大部分保留在卵巢内。在卵黄发生盛期,卵巢合成的卵黄原蛋白为脂肪体合成的卵黄原蛋白的20%。 相似文献
19.
Takao Yokota David R. Reeve Alan Crozier 《Bioscience, biotechnology, and biochemistry》2013,77(10):2091-2094
The synthesis of 2,3-(3H)-gibberellin A9 (GA9) with a specific activity of 47 Ci mmole?1 is described. △2,3GA9 methyl ester epoxide was converted to (3H)-GA9 methyl ester epoxide using carrier-free tritium gas. This product was de-epoxidized then hydrolysed to yield (3H)-GA9. 相似文献
20.
Thomas G. Wilson 《Development genes and evolution》1982,191(4):257-263
Summary It is known from previous work that juvenile hormone (JH) is required to initiate vitellogenin uptake into maturing oocytes ofDrosophila melanogaster, but additional requirements for this hormone during oocyte maturation have not been fully understood. To determine if early vitellogenic oocytes (stages 8 and 9) require JH for continued development, these oocytes were transplanted toDrosophila female and male hosts which were rendered deficient in JH by three methods. Implanted stage 9 and usually stage 8 oocytes were found to degenerate in JH-deficient hosts unless ZR-515, a JH analogue, was applied to the host shortly after implantation.These results were confirmed during in situ ovary development. JH deficiency was produced in gravid females, and ovaries examined at subsequent time intervals were found to be deficient in stage 8–10 oocytes as early as 6 h after treatment. Degenerating oocytes corresponding to these stages were commonly found. ZR-515 prevented oocyte degeneration during at least the first 8 h and continued to support stage 8–10 oocyte development 24 h after application to these females. The results suggest that JH is required not only for initiation but also for continuation of vitellogenin uptake and oocyte development. 相似文献