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1.
Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex
materials in a vacuum. It is widely applied to the discrimination of closely related microbial strains. Leaf samples from
eight cultivars (‘Apricot Delight’, ‘Cooler Grape’, ‘Cooler Peppermint’, ‘Equator Grape’, ‘Equator Rose’, ‘Equator White’,
‘Equator White Eye’, and ‘Little Bright Eye’) of Catharanthus roseus were subjected to PyMS for spectral fingerprinting. Discriminant analysis (DA) of PyMS data enabled us to assign these cultivars
to discrete clusters. A hierarchical dendrogram based on DA provided a possible relationship among them that was in general
agreement with a previously reported classification of the cultivars based on DNA fingerprints. Furthermore, those belonging
to the same ‘series’ were grouped into a single cluster, which previously could not be achieved through similar approaches
based on Fourier transform infrared spectroscopy or 1H NMR data. Overall results suggest that chemical differences (i.e., in pyrolysate composition) among cultivars, as detected
by mass spectrometry, reflect their genetic variation. 相似文献
2.
This study analyzed genetic differences of 19 cultivars selected from somaclonal variants of Syngonium podophyllum Schott along with their parents as well as seven additional Syngonium species and six other aroids using amplified fragment length polymorphism (AFLP) markers generated by 12 primer sets. Among the 19 somaclonal cultivars, ‘Pink Allusion’ was selected from ‘White Butterfly’. Tissue culture of ‘Pink Allusion’ through organogenesis resulted in the development of 13 additional cultivars. Self-pollination of ‘Pink Allusion’ obtained a cultivar, ‘Regina Red Allusion’, and tissue culture propagation of ‘Regina Red Allusion’ led to the release of five other cultivars. The 12 primer sets generated a total of 1,583 scorable fragments from all accessions, of which 1,284 were polymorphic (81.9%). The percentages of polymorphic fragments within ‘White Butterfly’ and ‘Regina Red Allusion’ groups, however, were only 1.2% and 0.4%, respectively. Jaccard's similarity coefficients among somaclonal cultivars derived from ‘White Butterfly’ and ‘Regina Red Allusion’, on average, were 0.98 and 0.99, respectively. Seven out of the 15 cultivars from the ‘White Butterfly’ group and three out of six from the ‘Regina Red Allusion’ group were clearly distinguished by AFLP analysis as unique fragments were associated with respective cultivars. The unsuccessful attempt to distinguish the remaining eight cultivars from the ‘White Butterfly’ group and three from the ‘Regina Red Allusion’ group was not attributed to experimental errors or the number of primer sets used; rather it is hypothesized to be caused by DNA methylation and/or some rare mutations. This study also calls for increased genetic diversity of cultivated Syngonium as they are largely derived from somaclonal variants. 相似文献
3.
Zhentu Ying Thomas L. Davenport Taifang Zhang Raymond J. Schnell Cecile L. Tondo 《Plant Molecular Biology Reporter》2009,27(3):374-380
‘Hass’ is the most popular avocado (Persea americana Mill.) cultivar in the world. It has been characterized as a crop requiring cross-pollination. However, the potential extent
of self-pollination and the most effective pollen donors (best cross-pollinizing cultivars) have not been determined. In this
study, 56 markers were screened against ‘Hass’ and nine commonly used pollinizing cultivars grown in southern California:
‘Bacon,’ ‘Ettinger,’ ‘Fuerte,’ ‘Harvest,’ ‘Lamb Hass,’ ‘Marvel,’ ‘Nobel,’ ‘Sir Prize,’ and ‘Zutano.’ Seventeen microsatellite,
i.e., simple sequence repeat (SSR) markers, were found to be very promising for paternity analysis. Four highly informative
SSR markers were selected to accurately and unequivocally identify pollen parents of ‘Hass’ fruit from an orchard interplanted
with these pollinizing cultivars. From 2003 to 2006, 7,984 ‘Hass’ fruit were analyzed for their paternity. Overall, the pollen
parents of 99.55% of the analyzed fruit could be unequivocally identified with a single multiplex polymerase chain reaction
(PCR). Only 36 fruits (<0.45%) required a second PCR reaction to reach unequivocal identification of the pollen parents. 相似文献
4.
Ying Li Yuping Song Gongjun Shi Jianjun Wang Xilin Hou 《Acta Physiologiae Plantarum》2009,31(1):155-162
Changes in ascorbic acid content and antioxidant enzyme activities were investigated in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) leaves of ‘Wutacai’ and ‘Erqing’ exposed to excess copper (Cu). Cu treatment reduced the fresh weight of shoot and
root by 57% and 46% in ‘Wutacai’, and 60 and 54% in ‘Erqing’, respectively. The accumulation of copper in leaves was higher
in ‘Wutacai’ than that in ‘Erqing’. Compared to the control, ascorbic acid (AsA) contents were significantly decreased after
copper treatment in both cultivars, while they were higher in ‘Wutacai’ than in ‘Erqing’, which may explain the higher copper-tolerance
of ‘Wutacai’ with higher copper accumulation. The higher AsA contents of ‘Wutacai’ resulted from their lower activities of
degrading enzymes, such as ascorbate oxydase (AAO) and ascorbate peroxidase (APX), as well as the increasing activity of dehydroascorbate
reductase (DHAR) after copper treatment compared with ‘Erqing’. Copper stimulated superoxide dismutase (SOD) activity in both
cultivars, but for catalase (CAT), there was little difference between both cultivars. Peroxidases (POD) activity was decreased
after copper treatment in ‘Erqing’, while in ‘Wutacai’, it was significantly increased at 14 days, and POD activity was higher
in ‘Wutacai’ than that in ‘Erqing’ at 21 and 28 days. Therefore, the induced increasing activity of POD in ‘Wutacai’ also
played an important role in its copper tolerance. 相似文献
5.
Mustapha Ennajeh Ahmadou Mohamed Vadel Habib Khemira 《Acta Physiologiae Plantarum》2009,31(4):711-721
In this study, we compared the efficacy of defense mechanisms against severe water deficit in the leaves of two olive (Olea europaea L.) cultivars, ‘Chemlali’ and ‘Meski’, reputed drought resistant and drought sensitive, respectively. Two-year old plants
growing in sand filled 10-dm3 pots were not watered for 2 months. Changes in chlorophyll fluorescence parameters and malondialdehyde content as leaf relative
water content (RWC) decreased showed that ‘Chemlali’ was able to maintain functional and structural cell integrity longer
than ‘Meski’. Mannitol started to accumulate later in the leaves of ‘Chemlali’ but reached higher levels than in the leaves
of ‘Meski’. The latter accumulated several soluble sugars at lower dehydration. ‘Chemlali’ leaves also accumulated larger
quantities of phenolic compounds which can improve its antioxidant response. Furthermore, the activity of three antioxidant
enzymes catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) increased as leaf RWC decreased. However, differences
were observed between the two cultivars for CAT and POD but not for APX. The activity of the first two enzymes increased earlier
in ‘Meski’, but reached higher levels in ‘Chemlali’. At low leaf hydration levels, ‘Chemlali’ leaves accumulated mannitol
and phenolic compounds and had increased CAT and POD activities. These observations suggest that ‘Chemlali’ was more capable
of maintaining its leaf cell integrity under severe water stress because of more efficient osmoprotection and antioxidation
mechanisms. 相似文献
6.
Betelvine (Piper betle L., family Piperaceae) is an important, traditional and widely cultivated crop of India. The cultivators and consumers recognize
more than 100 cultivars (landraces) based on regional and organoleptic considerations, while in terms of phytochemical constituents
only five groups have been identified for all the landraces. Since betelvine is an obligate vegetatively propagated species,
genomic changes, if any, may have become ‘fixed’ in the landraces. We carried out random amplified polymorphic DNA (RAPD)
analysis in several landraces considered in four groups, namely, ‘Kapoori’, ‘Bangla’, ‘Sanchi’ and ‘Others’ in order to ascertain their genetic diversity. On the basis of the data from eleven RAPD primers, we distinguished genetic
variation within and among the four groups of landraces. The results indicate the’Kapoori’ group is the most diverse. The neighbour joining (NJ) tree after a bootstrap (500 replicate) test of robustness clearly shows
the four groups to be well separated. Interestingly, all known male or female betelvine landraces have separated in the NJ
tree indicating an apparent gender-based distinction among the betelvines. 相似文献
7.
Sibylle Stoeckli Karsten Mody Cesare Gessler Andrea Patocchi Mauro Jermini Silvia Dorn 《Tree Genetics & Genomes》2008,4(4):833-847
The rosy apple aphid (Dysaphis plantaginea), the leaf-curling aphid (Dysaphis cf. devecta) and the green apple aphid (Aphis pomi) are widespread pest insects that reduce growth of leaves, fruits and shoots in apple (Malus × domestica). Aphid control in apple orchards is generally achieved by insecticides, but alternative management options like growing
resistant cultivars are needed for a more sustainable integrated pest management (IPM). A linkage map available for a segregating
F1-cross of the apple cultivars ‘Fiesta’ and ‘Discovery’ was used to investigate the genetic basis of resistance to aphids.
Aphid infestation and plant growth characteristics were repeatedly assessed for the same 160 apple genotypes in three different
environments and 2 consecutive years. We identified amplified fragment length polymorphism (AFLP) markers linked to quantitative
trait loci (QTLs) for resistance to D. plantaginea (‘Fiesta’ linkage group 17, locus 57.7, marker E33M35–0269; heritability: 28.3%), and to D. cf. devecta (‘Fiesta’ linkage group 7, locus 4.5, marker E32M39–0195; heritability: 50.2%). Interactions between aphid species, differences
in climatic conditions and the spatial distribution of aphid infestation were identified as possible factors impeding the
detection of QTLs. A pedigree analysis of simple sequence repeat (SSR) marker alleles closely associated with the QTL markers
revealed the presence of the alleles in other apple cultivars with reported aphid resistance (‘Wagener’, ‘Cox’s Orange Pippin’),
highlighting the genetic basis and also the potential for gene pyramiding of aphid resistance in apple. Finally, significant
QTLs for shoot length and stem diameter were identified, while there was no relationship between aphid resistance and plant
trait QTLs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Effects of exogenous B supply on growth, B accumulation and distribution of two navel orange cultivars 总被引:3,自引:0,他引:3
Ou Sheng Shang W. Song Yun J. Chen Shu A. Peng Xiu X. Deng 《Trees - Structure and Function》2009,23(1):59-68
To investigate the effects of boron (B) on growth, B concentration and distribution of two navel orange cultivars, ‘Newhall’
(Citrus sinensis Osbeck) and ‘Skagg’s Bonanza’ (Citrus sinensis Osbeck) grafted on the rootstock trifoliate orange [Poncirus trifoliata (L.) Raf.], B at five levels was exogenously supplied to 1-year-old grafted plants of both cultivars under greenhouse conditions.
Plants were grown in sand:perlite (1:1, v/v) medium and were irrigated every 2 days with half-strength Hoagland’s No. 2 nutrient
solutions containing different B, 0.01, 0.05, 0.10, 0.25 and 2.50 mg l−1 (0.25 and 2.50 mg l−1 were considered as control and excess B treatment, respectively, and the other three B levels were considered as low B treatments).
After treatments for 183 days, leaves (from basal, middle, upper parts of the shoots), stem of scion, stem of rootstock and
root were separately sampled. Our results showed that plant growth (plant height, root volume and dry weights of various parts)
was inhibited in response to low or excess B supplies in both cultivars. It was found that B concentrations in the upper leaves
of both cultivars were substantially higher than those in the basal leaves when low concentrations (≤0.05 mg l−1) of exogenous B were applied, suggesting that B was preferentially translocated to the upper-younger leaves to support their
growth. Analysis of B distribution in different parts indicated that translocation of B from the root to the scion’s shoots
(stems and leaves of scion) may be restricted upon exposure to low B conditions. When B was inadequately supplied, growth
of ‘Skagg’s Bonanza’ was better than ‘Newhall’, implying that the former cultivar was more tolerant to low B status, which
may be due to the higher efficiency of B translocation from the root to the scion’s shoots. However, when the plants were
treated with excess B (2.50 mg l−1), both cultivars showed a similar degree of B toxicity. The probability of scion–rootstock interactions in relation to the
differential responses of growth and different efficiency of B translocation involved in the two orange cultivars following
the long-term low B stress were discussed. 相似文献
9.
P. K. Subudhi S. Nandi C. Casal S. S. Virmani N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):941-949
The cytoplasmic genetic male-sterile (CMS) lines developed at the International Rice Research Institute are valuable in producing
tropical rice hybrids. Efficient use of CMS lines in hybrid rice production will depend on their level of genetic diversity.
Aside from morphological characterization, molecular analysis based on DNA markers can provide information on the genetic
diversity of the germplasm. The Amplified Fragment Length Polymorphism (AFLP) technique was used to fingerprint 71 CMS lines
and four rice cultivars, ‘IR64’, ‘Azucena’, ‘IR74’, and ‘FR13A’. Eleven primer pair combinations specific to the enzymes PstI and MseI were used to generate 530 AFLP markers, 176 of which were polymorphic. Each CMS line revealed a distinct fingerprint. The
AFLP marker-based dendrogram depicted genetic variation among the CMS lines. The CMS lines developed in japonica background
grouped with ‘Azucena’, a japonica cultivar. None of the CMS lines clustered with ‘FR13A’, a flood-tolerant traditional indica
variety. ‘IR64’ was found to be distinct from the other indica CMS lines and clustered with lines developed in its background.
The grouping of CMS lines into a few groups is useful for breeders in selecting genetically diverse CMS lines for hybrid rice
production and in avoiding test crossing every CMS line empirically. This study demonstrated that AFLP is a powerful and reliable
tool in determining the genetic relationships and in producing distinct fingerprints of rice cultivars.
Received: 20 December 1996 / Accepted: 9 October 1997 相似文献
10.
J. Perry Gustafson Xue-Feng Ma Viktor Korzun John W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):793-800
A consensus map of rye (Secale cereale L.) was constructed using JoinMap 2.0 based on mapping data from five different mapping populations, including ‘UC90’ × ‘E-line’,
‘P87’ × ‘P105’, ‘I0.1-line’ × ‘I0.1-line’, ‘E-line’ × ‘R-line’, and ‘Ds2’ × ‘RxL10’. The integration of the five mapping populations resulted in a 779-cM map
containing 501 markers with the number of markers per chromosome ranging from 57 on 1R to 86 on 4R. The linkage sizes ranged
from 71.5 cM on 2R to 148.7 cM on 4R. A comparison of the individual maps to the consensus map revealed that the linear locus
order was generally in good agreement between the various populations, but the 4R orientations were not consistent among the
five individual maps. The 4R short arm and long arm assignments were switched between the two population maps involving the
‘E-line’ parent and the other three individual maps. Map comparisons also indicated that marker order variations exist among
the five individual maps. However, the chromosome 5R showed very little marker order variation among the five maps. The consensus
map not only integrated the linkage data from different maps, but also greatly increased the map resolution, thus, facilitating
molecular breeding activities involving rye and triticale. 相似文献
11.
When an indigenous insect becomes a pest, comparisons of performance of pest and non-pest populations on crop plants and of
genetic variation in that performance may provide insight into the evolution of pest populations. To measure such genetic
variation, 8–15 clones of the grape phylloxera (Daktulosphaira vitifoliae Fitch) were collected from wild grapevines in each of 3 geographically isolated sites (populations) and from commercial vineyards
in northern California. A complete life table was made for clonal replicates from populations collected from wild grapevines
on each of two commercial grape cultivars, the susceptibleVitis vinifera (L.) cultivar Cabernet Sauvignon, and the phylloxera-resistant rootstock ‘AxR # 1’. Variation in mean performance on these
two hosts was partitioned among clones within collection sites and among sites. Performance measures included an individual
analog to the intrinsic rate of increase (r), age at first oviposition, fecundity in the first ten days of reproduction, total fecundity, and longevity. The overall
performance of phylloxera from the wild grapevines on the resistant cultivar AxR # 1 was greater than or equal to that on
the susceptible cultivar Cabernet Sauvignon. There was significant variation among clones within populations from wild grapes
in the rate of increase on ‘AxR # 1’ and marginally significant clonal variation in some of the component paramters. There
was no significant variation among clones within populations on ‘Cabernet Sauvignon’ and no significant differences between
populations on either crop in any trait.
In a second experiment we compared the relative performance of 15–17 clones from wild grapevines and from commercial vineyards
when reared on ‘Cabernet Sauvignon’ and ‘AxR # 1’. Phylloxera from commercial vineyards had much higher overall performance
on ‘Cabernet Sauvignon’ than did phylloxera from the wild grapevines. Phylloxera from the commercial vineyard also had higher
performance on ‘Cabernet Sauvignon’ than on ‘AxR′ 1’ but the performance of the phylloxera from wild and commercial grapes
did not differ on ‘AxR # 1’.
Our results show that there is genetic variation in traits related to performance on a resistant rootstock within these indigenous
non-pest populations of phylloxera, but not among them. The pattern of performance of pest and non-pest populations on two
commercial cultivars suggests that current levels of phylloxera performance on crop cultivars are the result of adaptation
to those cultivars which has occurred while phylloxera has been associated with viticulture. Implications of these results
for understanding the recent adaptation of phylloxera to ‘AxR # 1’ in California are also discussed. 相似文献
12.
Experiments were conducted to quantify parasitism of Colorado potato beetle,Leptinotarsa decemlineata (Say), by the egg parasitoid,Edovum puttleri Grissell, on 3 different cultivars of eggplant,Solanum melongena L. Levels of parasitism were higher (P<0.05) on ‘Black Pride’ than on other cultivars. The percentage of egg masses that
were parasitized was 1.2-fold higher (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’. The number of eggs per
mass that were parasitized was 1.3- and 1.4- fold greater (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’,
respectively. The percentage of eggs that were parasitized per mass and percentage of emerged adult parasitoids did not differ
(P>0.05) among cultivars; between 2.1- to 2.6- fold more females than males emerged from eggs on all cultivars during the
growing season.Edovum puttleri suppressed the 2nd generation ofL. decemlineata on ‘Black Pride’ and ‘Harris Special’, but did not suppress populations on ‘White’.
相似文献
13.
Tadeusz Zaj c Stanisław Grzesiak Bogdan Kulig Milan Polá ek 《Acta Physiologiae Plantarum》2005,27(4):549-558
In three year field experiments (2001 – 2003) the growth, yield and productivity of 8 flax cultivars were compared. Cultivars
‘AC Linora’, ‘Flanders’, ‘Linola™ 947’, ‘Norlin’ and ‘Omega’ were obtained from Canada, ‘Barbara’ and ‘Hungarian Gold’ from
Hungary and ‘Opal’ from Poland. Apart from the estimation of the yield of aboveground parts dry matter and seed yield the
determinations of the primary index value of growth analysis were done and on their basis the indices LAI, LAD, RGR, CGR and
HI were calculated.
The obtained yield results of the examined flax cultivars show significant genotypic — environmental relationships pertaining
to the dynamics of dry matter accumulation and the amount of seed yield. Meteorological conditions in the successive years
significantly influenced the particular phases of growth and development of cultivars and the factor which increased the amount
of dry matter was the air temperature during the period of plant emergence — budding. During the vegetative season with a
large amount of rainfall the average seed yield was about 40 % lower than compared with a year of average precipitation and
a warm second part of the second period of flax vegetation. Among the analyzed cultivars a stable yield in all the years was
characteristic for cultivars ‘Flanders’, ‘Barbara’ and ‘AC Linora’ (that cultivar, however, during a wet year yielded at a
low level). The assimilation leaf surface of the linseed quickly increased during the period from budding to flowering and
the accumulation of dry matter of the aboveground parts lasted up to the green maturity. In the successive years of the experiment
there were observed significant (linear or logarithmic regressions) relationship between the yield of dry matter and the indices
of growth analysis. The biggest values of the CGR indicator were observed for the period from budding to flowering. The maintaining
of a high CGR value after plant flowering in the year with a favourable course of climatic parameters was beneficial for a
better yield of all flax cultivars. The low values of the RGR index after flowering of cultivar ‘Hungarian Gold’ and ‘Opal’
strictly corresponded to their low yield of seed and straw biomass. 相似文献
14.
Shimpei Uraguchi Masako Kiyono Takuya Sakamoto Izumi Watanabe Katsuji Kuno 《Planta》2009,230(2):267-276
The contributions of cadmium (Cd) accumulation in cell walls, antioxidative enzymes and induction of phytochelatins (PCs)
to Cd tolerance were investigated in two distinctive genotypes of black oat (Avena strigosa Schreb.). One cultivar of black oat ‘New oat’ accumulated Cd in the leaves at the highest concentration compared to another
black oat cultivar ‘Soil saver’ and other major graminaceous crops. The shoot:root Cd ratio also demonstrated that ‘New oat’
was the high Cd-accumulating cultivar, whereas ‘Soil saver’ was the low Cd-accumulating cultivar. Varied levels of Cd exposure
demonstrated the strong Cd tolerance of ‘New oat’. By contrast, low Cd-accumulating cultivar ‘Soil saver’ suffered Cd toxicity
such as growth defects and increased lipid peroxidation, even though it accumulated less Cd in shoots than ‘New oat’. Higher
activities of ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1. 15. 1. 1) were observed in the leaves of
‘New oat’ than in ‘Soil saver’. No advantage of ‘New oat’ in PCs induction was observed in comparison to Cd-sensitive cultivar
‘Soil saver’, although Cd exposure increased the concentration of total PCs in both cultivars. Higher and increased Cd accumulation
in cell wall fraction was observed in shoots of ‘New oat’. On the other hand, in ‘Soil saver’, apoplasmic Cd accumulation
showed saturation under higher Cd exposure. Overall, the present results suggest that cell wall Cd accumulation and antioxidative
activities function in the tolerance against Cd stress possibly in combination with vacuolar Cd compartmentation. 相似文献
15.
Peroxidase activity of red raspberry canes was dependent on the cultivar and influenced the subsequent lignification. After
inoculation with Didymella applanata, responsible for the spur blight cane disease, the activity of soluble cytoplasmic enzyme increased in the moderately resistant
‘Latham’ and susceptible ‘Malling Promise’, similarly for syringaldazine and guaiacol as hydrogen donors. Systemic induction
found in ‘Latham’ was recognized as a symptom of defence mechanism responsible for fungal restriction. Locally enhanced peroxidase
activity in the ‘M.Promise’ tissues was related to the local lignification and/or may be associated with the loss of cell
integrity caused by pathogen penetration. Pathogen-induced changes of cell wall peroxidases were similar in both cultivars
mentioned above. No influence of the infection was found in the high susceptible Zeva cultivar.
Using native-PAGE analysis and horizontal starch electrophoresis of soluble fraction five constitutive acidic isoperoxidases
were detected in ‘Latham’ and three in ‘M. Promise’. The infection process was accompanied by the appearance of two new anodic
isoforms. 相似文献
16.
Kathryn Kamo Janet Chen Roger Lawson 《In vitro cellular & developmental biology. Plant》1990,26(4):425-430
Summary Inflorencence stalks from greenhouse-grownGladiolus plants of the cultivars ‘Blue Isle’ and ‘Hunting Song’ cultured on a Murashige and Skoog basal salts medium supplemented
with 53.6 μM 1-napthaleneacetic acid formed a compact, not friable type of callus that regenerated plantlets. Cormel slices and intact
plantlets of three cultivars (‘Peter Pears’, ‘Rosa Supreme’, ‘Jenny Lee’) propagated through tissue culture formed a friable
type of callus when cultured on Murashige and Skoog basal salts medium supplemented with 2,4-dichlorophenoxyacetic acid. This
friable callus readily formed a cell suspension when the callus was placed in a liquid medium. Plants were regenerated from
two-month-old suspension cell cultures of the commercial cultivar ‘Peter Pears’ after the suspension cells had been cultured
on solid medium. 相似文献
17.
M. J. van Steenis K. A. M. H. El-Khawass 《Entomologia Experimentalis et Applicata》1995,76(2):121-131
Life table data forAphis gossypii Glover (Homoptera: Aphididae), an important pest in glasshouse cucumber crops, were studied at 20, 25 and 30°C on two cucumber
cultivars (Cucumis sativus L.) in controlled climate cabinets. The development time on the cucumber cv. ‘Sporu’ ranged from 4.8 days at 20°C to 3.2
days at 30°C. Immature mortality was approximately 20% and did not differ between temperatures. Most mortality occurred during
the first instar. Reproduction periods did not differ among temperatures, but at 25 and 30°C more nymphs were produced (65.9
and 69.8 nymphs/♀, respectively) than at 20°C (59,9 nymphs/♀) because of a higher daily reproduction. Intrinsic rate of increase
was greatest at 25°C (r
m
=0.556 day−1). At 20 and 30°C the intrinsic rate of increase was 0.426 and 0.510, respectively. On cv. ‘Aramon’, the development time
ofA. gossypii was approximately 20% longer at all temperatures. Immature mortality did not differ between the two cultivars. The intrinsic
rate of increase on cv. ‘Aramon’ was 15% smaller than on cv. ‘Sporu’. The use of cucumber cultivars partially resistant to
aphids is discussed in relation to biological control of cotton aphid in glasshouses. Development time and immature mortality
on leaves of the middle and upper leaf layer of glasshouse grown cucumber plants (cv. ‘Aramon’) were comparable to development
in the controlled climate cabinets. On the lower leaves immature mortality was much higher (approximately 82%) than on leaves
of the middle (24.0%) and upper leaf layer (24.5%). Reproduction was less on the lower leaf layer (45.9, 70.5 and 70.1 nymphs/♀
on leaves of the lower, middle and upper leaf layer, respectively).
Aphids, successfully parasitized byAphidius colemani Viereck (Hymenoptera: Braconidae) only reproduced when they were parasitized after the third instar. Fecundity was 0.1 to
0.9 and 10.5 to 13.3 nymphs/♀ for aphids parasitized in the fourth instar or as adults, respectively. Reproduction of aphids
that were stung but survived the attack was lower than for aphids not stung. Average longevity of these aphids was equal to
the longevity of aphids not stung byA. colemani. 相似文献
18.
Response of twenty eight cultivars of durum wheat (Triticum turgidum var. durum) to immature embryo culture, callus production and in vitro salt tolerance was evaluated. For assessment of cultivars to salt tolerance, growing morphogenic calli were exposed to different
concentrations of NaCl (0, 0.3, 0.6, 0.9, 1.2, 1.5, 1.8 and 2.1% w/v) added to the culture medium during two subsequent subcultures
(4 weeks each). Comparison of cultivars for callus induction from immature embryo was based on callus induction frequency
and fresh weight growth of callus (FWG). While, for salt tolerance, the relative fresh weight growth (RFWG) and necrosis percent
of callus were used. There were significant differences among cultivars for potential of regeneration from immature embryo,
and ‘Shahivandi’ a native durum wheat cultivar originating from western Iran was superior among the cultivars tested. The
FWG distinguished cultivars more than callus induction frequency did for callus induction evaluation. Hence, a range of FWG
from 1.23 to 14.65 g was observed in ‘Mexical-75’ and ‘Omrabi-5’ cultivars, respectively. Growing calli derived from cultivars
‘PI 40100’ and ‘Dipper-6’ showed superiority for tolerating salinity under in vitro conditions.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
19.
Benzothiadiazole (BTH) was found to be highly effective in increasing resistance of two poinsettia cultivars — ‘Coco White’
and ‘Malibu Red’, moderately susceptible to the fungus Botrytis cinerea. BTH applied at a concentration of 0.3 mM on the discs cut out from the leaves of these poinsettia cultivars reduced disease
symptoms by more than 60 % in comparison to the control discs treated with water and exposed to infection.
It was also observed that the applied inducer at a concentration of 0.03 and 0.3 mM had a favourable influence on the increase
of poinsettia systemic resistance of SAR type (systemic acquired resistance). The effectiveness of BTH was much less when
disease development was examined on detached leaves (a 20 % reduction of lesion area) in comparison with a pronounced inhibition
of grey mould development on intact leaves of previously induced plants (a 80 % protection of intact plants).
Benzothiadiazole in the concentration range from 0.03 to 1.4 mM added to in vitro agar medium was not found to have an inhibitory influence on Botrytis cinerea mycelium growth and sporulation. 相似文献
20.
Taxonomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data 总被引:4,自引:0,他引:4
Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cells are analyzed. Leaf samples of seven higher plant species and varieties were subjected to FTIR to determine whether plants can be discriminated phylogenetically on the basis of biochemical profiles. A hierarchical dendrogram based on principal component analysis (PCA) of FTIR data showed relationships between plants that were in agreement with known plant taxonomy. Genetic programming (GP) analysis determined the top three to five biomarkers from FTIR data that discriminated plants at each hierarchical level of the dendrogram. Most biomarkers determined by GP analysis at each hierarchical level were specific to the carbohydrate fingerprint region (1,200–800 cm–1) of the FTIR spectrum. Our results indicate that differences in cell-wall composition and structure can provide the basis for chemotaxonomy of flowering plants.Abbreviations FTIR Fourier transform infrared spectroscopy - GP Genetic programming - PCA Principal component analysis - PyMS Pyrolysis mass spectrometry 相似文献