共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
5.
6.
Interferon-responsive regulatory elements in the promoter of the human 2'',5''-oligo(A) synthetase gene. 总被引:21,自引:5,他引:16
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The interferon (IFN)-activated human 2',5'-oligo(A) synthetase E gene contains 11 RNA starts and lacks TATA and CAAT signals. DNA sequences around the promoter make the expression of the chloramphenicol acetyltransferase gene (CAT) inducible over 20-fold by IFN. A 72-base-pair segment (E-IRS) immediately upstream of the RNA starts was defined as being required for IFN-activated expression of the E-gene promoter-CAT constructs and acts in a position-independent manner. It also confers IFN-activated enhancement to the herpes simplex virus thymidine kinase promoter. On this promoter, the 5' part of the E-IRS functions as a constitutive enhancer, while the last 16 base pairs of the E-IRS is sufficient to give IFN-induced expression. On the E-gene promoter, the constitutive enhancer and the IFN-activated sequence are both needed but can be separated. In addition, promoter competition experiments indicate a third regulatory region which helps to repress expression of the E gene in uninduced cells. 相似文献
7.
8.
K Nawa T Nakamura A Kumatori C Noda A Ichihara 《The Journal of biological chemistry》1986,261(36):16883-16888
9.
10.
11.
12.
13.
Structure of the rat alpha 1-acid glycoprotein gene. 总被引:4,自引:2,他引:2
Y C Liao J M Taylor J L Vannice G A Clawson E A Smuckler 《Molecular and cellular biology》1985,5(12):3634-3639
14.
D W Crabb P M Stein K M Dipple J B Hittle R Sidhu M Qulali K Zhang H J Edenberg 《Genomics》1989,5(4):906-914
15.
16.
17.
18.
19.
20.