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1.
We studied the histochemical distribution of zinc in rat epididymis using a sulphide-silver method. In the supranuclear cytoplasm of the principal cells that line the epididymis of rats, varying amounts of sulphide-silver-reactive zinc were visualized. In adult mating rats, significant amounts of zinc were found in the proximal portion of the epididymis, whereas in non-mating, mature and immature young rats, this heavy metal was most prominent in the distal portion of this organ. In all of the rats studied, zinc was sparsely distributed in the intermediate portion of the epididymis. From these results, it can be assumed that the zinc present in the epithelial lining of rat epididymis plays an important role in the maturation of spermatozoa. The present results represent a useful contribution to our understanding of the functional morphology of rat epididymis. 相似文献
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Histochemical demonstration of zinc ions in human epididymis using autometallography 总被引:1,自引:0,他引:1
Meredin Stoltenberg Lars Lund Soren Juhl Gorm Danscher Erik Ernst 《Journal of molecular histology》1997,29(10):721-726
Summary A recently described autometallographic technique, allowing demonstration of chelatable zinc in human biopsy material, was
applied to cryostat sections from biopsies of human epididymis. Sections from the rat epididymis were used as control materials
to examine the quality of the method compared with a previously used autometallographic method. The human epididymis exhibits
heavy staining in the head of the epididymis and only small amounts of zinc in the body and tail of the organ. The zinc staining
was found in the apical part of the ciliated cells and in the lumen. The present technique can be used to localize zinc ions
at ultrastructural levels. Zinc grains were localized in lysosome-like bodies and secretory granules of the ciliated cells.
The luminal staining was present as free, evenly dispersed zinc grains or attached to sperm cells and stereocilia in the lumen.
The large differences in staining patterns along the epididymal tract in humans and rats suggest that zinc ions are important
for the maturation of sperm cells
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
3.
Meredin Stoltenberg Erik Ernst Arne Andreasen Gorm Danscher 《The Histochemical journal》1996,28(3):173-185
Summary In the present study, the autometallograpic zinc sulphide technique, an improved version of the original Timm sulphide-silver method, was used. This technique reveals a particular pool of ionic zinc that is chelatable by diethyldithiocarbamate. At the light microscopical level, no reaction for zinc was found in tissues of young prepubertal rats. In adult mating and non-mating rats low zinc staining was found in the head and intermediate epididymis whereas the tail of the epididymis demonstrated high levels of zinc ions. Sections from the epididymal tail revealed a compartmentalization, based on pronounced differences in staining intensity along the epididymal ducts. At higher magnification zinc ions were found in the apical part of the principal cell and in the lumen. At the ultrastructural level autometallographic grains were located in vesicles and in lysosome-like structures of the apical parts of the principal cells. The luminal grains were found either associated with sperm cells, with the surface of the large microvilli (stereocilia), or free in the seminal fluid. The variation in content of zinc ions in the epididymal epithelium and lumen suggests that zinc ions are secreted into the lumen from the epididymal tail and may somehow be involved in maturation of the sperm cells. 相似文献
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Y. Fujii K. Shimizu M. Satoh M. Fujita Y. Fujioka Y. Li Y. Togashi N. Takeichi K. Nagashima 《Histochemistry and cell biology》1993,100(4):249-256
Livers of LEC rats were histochemically stained for copper according to the modified Timm's method, which includes trichloroacetic acid (TCA) treatment. TCA pretreatment was effective in removing zinc and iron, leaving copper as the major metal in the liver. Hepatocytes in 3-month-old rats were stained intensely by the modified Timm's method, both in frozen sections and in paraffin-embedded specimens. The centrilobular hepatocytes were usually stained, but positive cells were also randomly distributed in the hepatic lobes, showing a mosaic pattern. The staining was intensified in 8- compared to 3-month-old LEC rats. In contrast hepatocytes from LEA rats, the normal counterpart of LEC rats, were faintly stained for copper. Proliferating cholangioles found in older LEC rats were shown to lack copper deposition, and hepatocellular carcinoma showed less copper deposits than the hepatocytes surrounding the tumor. The copper staining was augmented in livers of LEC rats subjected to copper-loading, but was less intense in the livers treated with d-penicillamine. The staining intensity under the various experimental conditions showed good correlation with the copper concentration. Lysosomal deposition of copper in hepatocytes was demonstrated by electron microscopic analysis for copper. Thus the modified Timm's method was shown to produce valuable results in demonstrating copper in LEC rat livers, providing important information for an understanding of the mechanism of copper deposition and hepatic disease of the animal. 相似文献
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T. Ueda Osamu Fujimori Azuma Tsukise Kazuyori Yamada 《Histochemistry and cell biology》1998,109(4):399-407
A variety of sialic acids contained in the rat epididymis were histochemically examined by means of lectin and pre-lectin
methods by light microscopy. Epididymides from adult male Sprague-Dawley rats were fixed in Bouin’s fluid and routinely embedded
in paraffin wax. Hydrated sections were subjected either to the lectin methods using biotinylated Limax flavus, Sambucus nigra, Sambucus sieboldiana or Maackia amurensis lectins or to the selective periodate oxidation–phenylhydrazine–thiocarbohydrazide–silver protein–physical development technique
with or without saponification. The present results revealed that principal cells in the initial segment and caput contain
sialic acid linked to α2,6-galactose/N-acetylgalactosamine, whereas those in the corpus and cauda include the sialic acidα2,3-galactose sequence. Narrow and clear
cells involve all the types of sialic acids examined. Basal and halo cells mainly contain sialic acidα2,3-galactose. 8- And/or
9-O-acetylated sialic acids were predominantly distributed in principal cells of the initial segment and proximal caput. These
findings are taken to indicate that various sialic acids in the epididymis could participate in different physiological functions
characteristic of the regions in this organ.
Accepted: 10 November 1997 相似文献
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Summary A histochemical method modified for ultrastructural studies of mercury induced changes is described. Rat neurons from areas known to be influenced by mercury are used as examples. The histochemical reaction, suggested to be caused by polymercury sulphide complexes, is localized to dense bodies where it is visible 14 days after initiation of peroral mercury treatment (20 mg HgCl2/l drinking water). 相似文献
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The localization of exopeptidase activities was demonstrated histochemically (by simultaneous azo coupling) on the visceral endoderm of whole unfixed yolk sacs of rats (12.5-18.5 days of gestation). For comparison, the topochemistry of exopeptidases was studied by conventional section histochemistry of frozen yolk sacs. The study of unfixed visceral yolk-sac epithelium showed that different artificial peptidase substrates (Ala-, Met-, Phe-, Leu-, alpha-Asp-, alpha-Glu-, gamma-Glu, Tyr-, Val-, Ser-, Arg- and Gly-Pro-MNA) are hydrolysed in the apical-cell membranes (membrane-bound peptidases) and, in a number of cells, within the cytoplasmic matrix. Section histochemistry showed that peptidase activities were almost only directed against gamma-Glu- and Gly-Pro-MNA at the cell apices. It is concluded that most of the exopeptidase activities in the apical cell membrane of the visceral yolk-sac epithelium are only demonstrable in unfixed yolk sacs. These activities are of great importance for the supplying of the embryo with amino acids. 相似文献
13.
Summary Male and female rat liver were studied during post-natal development. A correlation was found between biochemically determined hydroxylations and enzymhisto-chemically determined NADPH-nitro-BT reductase and Naphthol-AS-D esterase. No correlation was found between glucose-6-phosphate dehydrogenase or iso-citric acid dehydrogenase activity and hydroxylations. The difference in hydroxylating capacity between male and female rats may be caused by the fact that the number of cells with hydroxylating activity in the liver lobule, as judged by the NADPH-nitro-BT reductase and Naphthol-AS-D esterase activity, is higher in male than in female rats.List of Abbreviations NADH
reduced nicotinamide adenine dinucleotide
- NADPH
reduced nicotinamide adenine dinucleotide phosphate
- G6PD
glucose-6-phosphate dehydrogenase
- ICD
iso-citric acid dehydrogenase
- G6Pase
glucose-6-phosphatase
- NADPH
-nitro-BT red
- NADPH
Nitro-blue tetrazolium reductase
- SDH
succinic acid dehydrogenase
- TCA
trichloracetic acid 相似文献
14.
A histochemical method modified for ultrastructural studies of mercury induced changes is described. Rat neurons from areas known to be influenced by mercury are used as examples. The histochemical reaction, suggested to be caused by polymercury sulphide complexes, is localized to "dense bodies" where it is visible 14 days after initiation of peroral mercury treatment (20 mg HgCl2/l drinking water). 相似文献
15.
Summary The localization of exopeptidase activities was demonstrated histochemically (by simultaneous azo coupling) on the visceral endoderm of whole unfixed yolk sacs of rats (12.5–18.5 days of gestation). For comparison, the topochemistry of exopeptidases was studied by conventional section histochemistry of frozen yolk sacs. The study of unfixed visceral yolk-sac epithelium showed that different artificial peptidase substrates (Ala-, Met-, Phe-, Leu-, -Asp-, -Glu-, -Glu, Tyr-, Val-, Ser, Arg- and Gly-Pro-MNA) are hydrolysed in the apical-cell membranes (membrane-bound peptidases) and, in a number of cells, within the cytoplasmic matrix. Section histochemistry showed that peptidase activities were almost only directed against -Glu-and Gly-Pro-MNA at the cell apices. It is concluded that most of the exopeptidase activities in the apiccal cell membrane of the visceral yolk-sac epithelium are only demonstrable in unfixed yolk sacs. These activities are of great importance for the supplying of the embryo with amino acids.Supported by the Deutsche Forschungsgemeinschaft (SFB 105) 相似文献
16.
Histochemical activity of malate dehydrogenase (MDH: E.C. 1.1.1.37) in rat sperm mitochondria decreased during the epididymal transit of sperm. In an electrophoretic study two major MDH isozymes (MDH-A and MDH-B) were demonstrated in the sperm. The epididymal sperm showed two minor isozymes associated with MDH-A, while the testicular sperm did not. 相似文献
17.
F. Sinowatz M. Fischer R. Skolek-Winnisch J. A. Chandler 《The Histochemical journal》1979,11(1):103-109
Synopsis The histochemical localization of five glycosidases was studied in the epididymis of mature dogs. -Galactosidase showed a distinct to strong reaction in the epithelium of the ductuli efferentes and throughout the whole length of the ductus epididymidis. -N-Acetylglucosaminidase reactivity was weak in the initial segment, but increased significantly in the middle and terminal segment. The maximum -glucuronidase activity was found in the ductuli efferentes and in the initial segment. The -mannosidase reaction was weak in all segments except the middle segment where a distinct activity was seen. With the method employed, no -fucosidase activity could be detected. The physiological role of the glycosidases in the epididymis is discussed briefly. 相似文献
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Samuel H. Hori 《Histochemistry and cell biology》1966,7(4):297-302
Summary An attempt was made to locate the ATP: creatine phosphotransferase (creatine kinase, CKase) in rat skeletal muscle by a lead precipitation method. The muscle is not stained at all with creatine phosphate (CP), and only weakly with adenosine diphosphate (ADP) as substrate, while it hydrolyzes adenosine triphosphate (ATP) actively. Taking advantage of this fact, it is possible to demonstrate the CKase activity using both ADP and CP as substrate. The CKase activity thus obtained was located in various profiles of sarcoplasmic reticulum as well as in A bands, the staining being comparable to that obtained with ATP as substrate.A weak activity was found only in cisternal dilatations of sarcoplasmic reticulum when sections were incubated with ADP as substrate. 相似文献
20.
Histochemical and cytochemical demonstration of zinc cysteinate in the tapetum lucidum of the cat 总被引:1,自引:0,他引:1
T. Kohler 《Histochemistry and cell biology》1981,70(2):173-178
Summary Zinc cysteinate is shown histochemically and cytochemically in the tapetum lucidum of the cat. Heavy metal is demonstrated in the paraplasmic rods of the tapetal cells with a sulphide silver method whereas no such reaction can be found in the pigmented epithelium of the retina. These rods are also stained with silver methenamine indicating the presence of reducing groups which probably appeared after hydrolization of cysteine. 相似文献