共查询到20条相似文献,搜索用时 15 毫秒
1.
W. Patterson A. McDowell A. Hughes J. Bilotta 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2002,188(4):283-293
While some lower vertebrates, such as zebrafish, do not appear to possess anatomically separate pathways of processing visual information (such as M-pathways and P-pathways), it is believed that separate processing of the visual stimulus (such as luminance and chromatic processing) is a basic requirement of vertebrate vision. In this study, spectral sensitivity functions were obtained from electroretinogram responses to heterochromatic flicker photometry stimuli at several flicker rates, including a low flicker rate (2 Hz), in an attempt to predominantly stimulate chromatic processes and a high flicker rate (16 Hz), in an attempt to predominantly stimulate luminance processes. In addition, chromatic adaptation was used to isolate and examine the temporal properties of the different cone-type contributions to the electroretinogram response. Spectral sensitivity functions based on responses to heterochromatic stimuli of a low flicker rate appeared to receive both opponent and nonopponent contributions; however, when the stimulus flicker rate was high, spectral sensitivity appeared to be a function of only nonopponent mechanisms. Also, the differences in cone contributions to the spectral sensitivity functions across the different flicker rates appear to be related to the temporal properties of the cone contributions to the electroretinogram response. 相似文献
2.
M. Lehrer M. Wunderli M. V. Srinivasan 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1993,172(1):1-6
Freely flying honeybees were trained to discriminate a stimulus consisting of two alternating chromatic lights (heterochromatic flicker) from a steady mixture of the same two lights, using 3 different pairs of lights: blue-UV, UV-green, and green-UV. With each light pair, training to the heterochromatic flicker was conducted at several flicker frequencies, using experimentally naive bees in each training. In subsequent tests, the trained bees were given a choice between the two lights that constituted the flicker, presented steady, as well as between either of them and the steady mixture. We find that bees trained to particular frequencies of heterochromatic flicker prefer one of the component lights over the other as well as over the steady mixture, suggesting that the colour they perceive in the heterochromatic flicker to which they have been trained is shifted in the direction of one of the lights contained in the flicker. The colour shift occurs at flicker frequencies that depend on the pair of lights used. We propose that the shift is generated by an effect similar to the Brücke-Bartley phenomenon known from human vision. This effect is based on the enhancement of the photoreceptors' response upon onset of stimulation, causing an intermittent light to appear brighter than a steady light of identical physical intensity. We propose that the degree of enhancement might differ among the 3 spectral classes of photoreceptor, causing the colour perceived in a heterochromatic flicker to differ from that perceived in a steady mixture of its two light components. 相似文献
3.
《Archives of biochemistry and biophysics》1985,240(1):246-256
Previous work on adenosine transport has always had problems with the interference of adenosine metabolism, due to its high metabolic rate and because the enzymes involved are consistently present in most tissues. A new experimental model for studying adenosine transport in human erythrocyte ghosts is presented in this work: Human erythrocyte ghosts were sealed in the presence of erythro-3(2-hydroxynonyl)adenine and P1-P5-di(adenosine)5′-pentaphosphate, inhibitors of adenosine deaminase and adenosine kinase, respectively. These ghosts proved to lack adenosine metabolism when incubated in [U-14C]adenosine at 10 μm concentration at 37 °C for 60 min. Ghosts were 99.4% sealed in the correct orientation and had constant intracellular water volume. With these characteristics, the erythrocyte ghost preparation has many advantages for studying adenosine transport without adenosine metabolism interference. Adenosine transport was studied following the technique of 24., 25. Experiments to study Zero-trans influx and efflux, equilibrium exchange, and infinite-trans influx and efflux are presented. Adenosine transport did not behave linearly in any of these experimental procedures. Adenosine basic kinetic constants, calculated according to the procedure of Lieb and Stein, were R1→-2 = 4.1 × 10−4, R2→-1 = 3.97 × 10−4, Ree = 1.94 × 10−4, Roo = 6.08 × 10−4, K1→-2 = 125.67 μm, andK2→-1 = 84.36 μm. Lieb and Stein rejection criteria were used to distinguish a simple pore from a simple carrier. The data accumulated indicate that adenosine transport is carried out by a system that satisfies the criteria used for the simple carrier model. Asymmetric behavior was observed indicating lower affinity of the carrier for adenosine influx, although Vmax values for influx and efflux were similar. 相似文献
4.
JOSHUA J. LAWLER DENIS WHITE† RONALD P. NEILSON‡ ANDREW R. BLAUSTEIN 《Global Change Biology》2006,12(8):1568-1584
Predicted changes in the global climate are likely to cause large shifts in the geographic ranges of many plant and animal species. To date, predictions of future range shifts have relied on a variety of modeling approaches with different levels of model accuracy. Using a common data set, we investigated the potential implications of alternative modeling approaches for conclusions about future range shifts and extinctions. Our common data set entailed the current ranges of 100 randomly selected mammal species found in the western hemisphere. Using these range maps, we compared six methods for modeling predicted future ranges. Predicted future distributions differed markedly across the alternative modeling approaches, which in turn resulted in estimates of extinction rates that ranged between 0% and 7%, depending on which model was used. Random forest predictors, a model‐averaging approach, consistently outperformed the other techniques (correctly predicting >99% of current absences and 86% of current presences). We conclude that the types of models used in a study can have dramatic effects on predicted range shifts and extinction rates; and that model‐averaging approaches appear to have the greatest potential for predicting range shifts in the face of climate change. 相似文献
5.
We propose a new computational model to predict amide proton chemical shifts in proteins. In addition to the ring-current,
susceptibility and electrostatic effects of earlier models, we add a hydrogen-bonding term based on density functional calculations
of model peptide–peptide and peptide–water systems. Both distance and angular terms are included, and the results are rationalized
in terms of natural bond orbital analysis of the interactions. Comparison to observed shifts for 15 proteins shows a significant
improvement over existing structure-shift correlations. These additions are incorporated in a new version of the SHIFTS program. 相似文献
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7.
Spectrin: the ghost in the machine 总被引:1,自引:0,他引:1
Thomas GH 《BioEssays : news and reviews in molecular, cellular and developmental biology》2001,23(2):152-160
It has long been speculated that spectrin, the actin crosslinking and molecular scaffold protein, is involved in the development of apicobasal polarity in epithelia. While spectrins can undoubtedly influence the protein content of specific membrane domains, recent genetic evidence indicates that this activity is not necessary for the establishment or maintenance of this axis. Instead, these studies point to critical roles in tissue stability and morphogenesis. A possible role in cellular contractility is highlighted in this review. 相似文献
8.
M. H. Ledlie N. A. J. Graham J. C. Bythell S. K. Wilson S. Jennings N. V. C. Polunin J. Hardcastle 《Coral reefs (Online)》2007,26(3):641-653
Cousin Island marine reserve (Seychelles) has been an effectively protected no-take marine protected area (MPA) since 1968 and was shown in 1994 to support a healthy herbivorous fish assemblage. In 1998 Cousin Island reefs suffered extensive coral mortality following a coral bleaching event, and a phase shift from coral to algal dominance ensued. By 2005 mean coral cover was <1%, structural complexity had fallen and there had been a substantial increase in macroalgal cover, up to 40% in some areas. No clear trends were apparent in the overall numerical abundance and biomass of herbivorous fishes between 1994 and 2005, although smaller individuals became relatively scarce, most likely due to the loss of reef structure. Analysis of the feeding habits of six abundant and representative herbivorous fish species around Cousin Island in 2006 demonstrated that epilithic algae were the preferred food resource of all species and that macroalgae were avoided. Given the current dominance of macroalgae and the apparent absence of macroalgal consumers, it is suggested that the increasing abundance of macroalgae is reducing the probability of the system reverting to a coral dominated state. 相似文献
9.
We examined the effects of random fluctuations and diffusion on regime shifts in bistable aquatic ecosystems, using a simple two-component reaction–diffusion model with nutrients and phytoplankton. The corresponding mean-field model generates two stable attractors: one is a nutrient-dominated (ND) state or a clear-water state, and the other is a phytoplankton-dominated (PD) state or a turbid-water state. According to our simulations of the reaction–diffusion model without fluctuations, either of two stable states can be dominant corresponding to the given parameters and the configuration of two states, meaning that the long-term coexistence of these two states is difficult. The dominant state expands gradually eliminating the other non-dominant one, which results in the homogeneous distribution of the dominant state in the whole lake. Further simulations including the effects of temporally changing fluctuations show that the regime shifts driven by external noise proceed even within a bistable region, which complicates the detection of hysteresis in real aquatic ecosystems. 相似文献
10.
Peter J. Mumby 《Coral reefs (Online)》2009,28(3):761-773
Caribbean coral reefs are widely thought to exhibit two alternate stable states with one being dominated by coral and the
other by macroalgae. However, the observation of linear empirical relationships among grazing, algal cover and coral recruitment
has led the existence of alternate stable states to be questioned; are reefs simply exhibiting a continuous phase shift in
response to grazing or are the alternate states robust to certain changes in grazing? Here, a model of a Caribbean forereef
is used to reconcile the existence of two stable community states with common empirical observations. Coral-depauperate and
coral-dominated reef states are predicted to be stable on equilibrial time scales of decades to centuries and their emergence
depends on the presence or absence of a bottleneck in coral recruitment, which is determined by threshold levels of grazing
intensity and other process variables. Under certain physical and biological conditions, corals can be persistently depleted
even while increases in grazing reduce macroalgal cover and enhance coral recruitment; only once levels of recruitment becomes
sufficient to overwhelm the population bottleneck will the coral-dominated state begin to emerge. Therefore, modest increases
in grazing will not necessarily allow coral populations to recover, whereas large increases, such as those associated with
recovery of the urchin Diadema antillarum, are likely to exceed threshold levels of grazing intensity and set a trajectory of coral recovery. The postulated existence
of alternate stable states is consistent with field observations of linear relationships between grazing, algal cover and
coral recruitment when coral cover is low and algal exclusion when coral cover is high. The term ‘macroalgal dominated’ is
potentially misleading because the coral-depauperate state can be associated with various levels of macroalgal cover. The
term ‘coral depauperate’ is preferable to ‘macroalgal dominated’ when describing alternate states of Caribbean reefs. 相似文献
11.
Employment of the C-banding procedure disclosed the presence of slightly stained bands in some chromosome regions of several Triturus species. These regions have been previously described in other organisms as 'grey C-bands'. Possible explanations for the presence of these bands in Triturus karyotypes are discussed. 相似文献
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For the biophysical study of membranes, a variety of model systems have been used to measure the different parameters and to extract general principles concerning processes that may occur in cellular membranes. However, there are very few reports in which the results obtained with the different models have been compared. In this investigation, we quantitatively compared the phase coexistence in Langmuir monolayers, freestanding bilayers and supported films composed of a lipid mixture of DLPC and DPPC. Two-phase segregation was observed in most of the systems for a wide range of lipid proportions using fluorescence microscopy. The lipid composition of the coexisting phases was determined and the distribution coefficient of the fluorescent probe in each phase was quantified, in order to explore their thermodynamic properties. The comparison between systems was carried out at 30 mN/m, since it is accepted that at this or higher lateral pressures, the mean molecular area in bilayers is equivalent to that observed in monolayers. Our study showed that while Langmuir monolayers and giant unilamellar vesicles had a similar phase behavior, supported films showed a different composition of the phases with the distribution coefficient of the fluorescent probe being close to unity. Our results suggest that, in supported membranes, the presence of the rigid substrate may have led to a stiffening of the liquid-expanded phase due to a loss in the degrees of freedom of the lipids as a consequence of the proximity of the solid material. 相似文献
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15.
Celik Kayalar 《The Journal of membrane biology》1979,45(1-2):37-42
Summary A mechanism is proposed for the translocation of protons through the hydrogen bonded peptide groups of a protein by means of keto-enol tautomerization. The model is discussed in relation to energy coupling in biomembranes. 相似文献
16.
The following facts are considered in connection with the problem of population polymorphism at heterochromatic regions of maize chromosomes: (a) variation (1–3 μm) of the heterochromatic region of nucleolus organizer (NO knob) in pollen mother cell at the pachytene stage; (b) presumably function-dependent variation of the degree of its compaction (from a compact state in the majority of plants to a puff-like state); (c) the presence of rearrangements in the NO knob region (duplications and deletion); and (d) homozygous (in all cases) state of the NO knob. Deletion is combined with alterations in the structure of chromosomal NO and the overall karyotype. It is assumed that inbreeding and MGEs influence the mutability of the NO locus and activation of the gene set controlling cytokinesis, chemical reduplication, and, possibly, rDNA amplification. The mutation was classified as a systemic mutation. The mechanisms of NO knob homozygotization in meiosis (mitosis) and the mechanisms of maintenance of the polymorphism at functionally inactive chromosome knob regions in maize populations are compared. 相似文献
17.
Kreiling JA Tamamori-Adachi M Sexton AN Jeyapalan JC Munoz-Najar U Peterson AL Manivannan J Rogers ES Pchelintsev NA Adams PD Sedivy JM 《Aging cell》2011,10(2):292-304
Chromatin is highly dynamic and subject to extensive remodeling under many physiologic conditions. Changes in chromatin that occur during the aging process are poorly documented and understood in higher organisms, such as mammals. We developed an immunofluorescence assay to quantitatively detect, at the single cell level, changes in the nuclear content of chromatin-associated proteins. We found increased levels of the heterochromatin-associated proteins histone macro H2A (mH2A) and heterochromatin protein 1 beta (HP1β) in human fibroblasts during replicative senescence in culture, and for the first time, an age-associated increase in these heterochromatin marks in several tissues of mice and primates. Mouse lung was characterized by monophasic mH2A expression histograms at both ages, and an increase in mean staining intensity at old age. In the mouse liver, we observed increased age-associated localization of mH2A to regions of pericentromeric heterochromatin. In the skeletal muscle, we found two populations of cells with either low or high mH2A levels. This pattern of expression was similar in mouse and baboon, and showed a clear increase in the proportion of nuclei with high mH2A levels in older animals. The frequencies of cells displaying evidence of increased heterochromatinization are too high to be readily accounted for by replicative or oncogene-induced cellular senescence, and are prominently found in terminally differentiated, postmitotic tissues that are not conventionally thought to be susceptible to senescence. Our findings distinguish specific chromatin states in individual cells of mammalian tissues, and provide a foundation to investigate further the progressive epigenetic changes that occur during aging. 相似文献
18.
Hughes TP Rodrigues MJ Bellwood DR Ceccarelli D Hoegh-Guldberg O McCook L Moltschaniwskyj N Pratchett MS Steneck RS Willis B 《Current biology : CB》2007,17(4):360-365
Many coral reefs worldwide have undergone phase shifts to alternate, degraded assemblages because of the combined effects of over-fishing, declining water quality, and the direct and indirect impacts of climate change. Here, we experimentally manipulated the density of large herbivorous fishes to test their influence on the resilience of coral assemblages in the aftermath of regional-scale bleaching in 1998, the largest coral mortality event recorded to date. The experiment was undertaken on the Great Barrier Reef, within a no-fishing reserve where coral abundances and diversity had been sharply reduced by bleaching. In control areas, where fishes were abundant, algal abundance remained low, whereas coral cover almost doubled (to 20%) over a 3 year period, primarily because of recruitment of species that had been locally extirpated by bleaching. In contrast, exclusion of large herbivorous fishes caused a dramatic explosion of macroalgae, which suppressed the fecundity, recruitment, and survival of corals. Consequently, management of fish stocks is a key component in preventing phase shifts and managing reef resilience. Importantly, local stewardship of fishing effort is a tractable goal for conservation of reefs, and this local action can also provide some insurance against larger-scale disturbances such as mass bleaching, which are impractical to manage directly. 相似文献
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