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We have isolated a cDNA clone (pSR132) representing a mRNA which accumulates in senescing carnation flower petals in response to ethylene. In vitro translation of RNA selected by hybridization with pSR132 indicated the mRNA encoded a polypeptide of approximately 36 kDa. This was confirmed by DNA sequence analysis, which predicted a peptide composed of 318 amino acids with a calculated molecular weight of 34.1 kDa. Comparison of the predicted peptide sequence of pSR132 with other proteins compiled in the NBRF data base revealed significant homology with carboxyphosphonoenolpyruvate mutase and phosphoenolpyruvate mutase from Streptomyces hygroscopicus and Tetrahymena pyriformis, respectively. These enzymes are involved in the formation of C-P bonds in the biosynthesis of phosphonates. C-P bonds are found in a wide range of organisms, but their presence or formation in higher plants has not been investigated. 相似文献
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R. Nichols 《Planta》1977,135(2):155-159
Production of endogenous ethylene from the styles, ovary and petals of pollinated and unpollinated flowers of Dianthus caryophyllus L. was measured. The rate of ethylene production of cut, unpollinated flowers aged in water at 18°C was low until the onset of petal wilting, when a rapid surge of ethylene occurred in all tissues. The flower ethylene production was evolved mostly from the styles and petals. The bases of petals from unpollinated, senescing flowers evolved ethylene faster and sometimes earlier than the upper parts. Treatment of cut flowers with propylene, an ethylene analogue, accelerated wilting of flower petals and promoted endogenous ethylene production in all flower tissues. Pollination of intact flowers also promoted endogenous ethylene production and caused accelerated petal wilting within 2–3 days from pollination. Although the data are consistent with the hypothesis that ethylene forms a link between pollination of the style and petal wilting, in the unpollinated flower the style and petals can evolve a surge of ethylene independently of each other, about the time when the petals irreversibly wilt. The results are discussed in relation to the role of ethylene in flower senescence. 相似文献
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A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques. The cDNA (2382 bp) contained an open reading frame of 1986 bp corresponding to 662 amino acids. The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively. Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA. 相似文献
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Molecular cloning and characterization of senescence-related genes from carnation flower petals 总被引:3,自引:3,他引:0
The senescence of carnation (Dianthus caryophyllus L.) flower petals is associated with increased production of ethylene which plays an important role in regulating this developmental event. Three senescence-related cDNA clones were isolated from a cDNA library prepared from mRNA isolated from senescing petals. These cDNAs are representative of two classes of mRNAs which increase in abundance in senescing petal tissue. The mRNA for one class is present at low levels during the early stages of development and begins to accumulate in mature petals prior to the increase in ethylene production. The accumulation of this mRNA is reduced, but not eliminated, in petals treated with aminooxyacetic acid, an inhibitor of ethylene biosynthesis, or silver thiosulfate, an ethylene action inhibitor. In contrast, expression of the second class of mRNAs appears to be highly regulated by ethylene. These mRNAs are not detectable prior to the rise in ethylene production and increase in abundance in parallel with the ethylene climacteric. Furthermore, expression of these mRNAs is significantly inhibited by both aminooxyacetic acid and silver thiosulfate. Expression of these mRNAs in vegetative and floral organs was limited to floral tissue, and predominantly to senescing petals. 相似文献
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The possibility that exopeptidases, i.e. aminopeptidases and carboxypeptidases, in addition to the previously studied endopeptidase
might also be developmentally regulated in daylily petals was examined. The level of leucine aminopeptidase and endopeptidase
activities changed after the flower was fully open while that of carboxypeptidase activity remained relatively unchanged throughout
senescence. Leucine aminopeptidase activity seemed to increase after the flower was fully open and peaked several hours earlier
than endopeptidase did. Taken together, it is postulated that leucine aminopeptidase might play a role in protein turnover
during flower opening and in the initiation of protein hydrolysis associated with petal senescence while the endopeptidase
could be responsible for the breakdown of the bulk of proteins at the later stages. The drop in leucine aminopeptidase activity
associated with the onset of daylily petal senescence was effectively halted by a cycloheximide treatment of cut daylily flowers
for 24 h which was previously shown to prolong the vase life of the flowers and prevent protein loss from the petals. Apart
from both being developmentally regulated in daylily petals, the leucine aminopeptidase activity and the previously studied
endopeptidase are different in several aspects. They appear to have different pH optima, 8 for leucine aminopeptidase and
6.2 for endopeptidase. Unlike the endopeptidase activity, no new leucine aminopeptidase isozymes appeared during petal senescence,
and the leucine aminopeptidase did not appear to belong to the cysteine class of proteolytic enzymes. 相似文献
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A cDNA clone encoding a lipase that is up-regulated in senescing leaves and flower petals has been isolated by screening an expression library. The abundance of the lipase mRNA increases as flowers and leaves begin to senesce, and expression of the gene is also induced by treatment with ethylene. Transgenic Arabidopsis plants in which levels of the senescence-induced lipase protein have been reduced show delayed leaf senescence. 相似文献
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Erin M. O’Donoghue Sheryl D. Somerfield Lyn M. Watson David A. Brummell Donald A. Hunter 《Planta》2009,229(3):709-721
Galactose was the major non-cellulosic neutral sugar present in the cell walls of ‘Mitchell’ petunia (Petunia axillaris × P. axillaris × P. hybrida) flower petals. Over the 24 h period associated with flower opening, there was a doubling of the galactose content of polymers
strongly associated with cellulose and insoluble in strong alkali (‘residual’ fraction). By two days after flower opening,
the galactose content of both the residual fraction and a Na2CO3-soluble pectin-rich cell wall fraction had sharply decreased, and continued to decline as flowers began to wilt. In contrast,
amounts of other neutral sugars showed little change over this time, and depolymerisation of pectins and hemicelluloses was
barely detectable throughout petal development. Size exclusion chromatography of Na2CO3-soluble pectins showed that there was a loss of neutral sugar relative to uronic acid content, consistent with a substantial
loss of galactose from rhamnogalacturonan-I-type pectin. β-Galactosidase activity (EC 3.2.1.23) increased at bud opening,
and remained high through to petal senescence. Two cDNAs encoding β-galactosidase were isolated from a mixed stage petal library.
Both deduced proteins are β-galactosidases of Glycosyl Hydrolase Family 35, possessing lectin-like sugar-binding domains at
their carboxyl terminus. PhBGAL1 was expressed at relatively high levels only during flower opening, while PhBGAL2 mRNA accumulation occurred at lower levels in mature and senescent petals. The data suggest that metabolism of cell wall-associated
polymeric galactose is the major feature of both the opening and senescence of ‘Mitchell’ petunia flower petals. 相似文献
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Daylily flowers (Hemerocallis hybrid, cv. Stella
d'Oro) senesce and die autonomously over a 24 h period after opening.
Investigations were performed to determine some of the mechanisms that lead
to death of the petals. The flowers are insensitive to ethylene, but
exogenous ABA prematurely upregulates events that occur during natural
senescence, such as loss or differential membrane permeability, increases
in lipid peroxidation and the induction of proteinase and RNase activities.
Furthermore, the same patterns of proteinase and RNase activities appearing
on activity gels during natural senescence are induced prematurely by ABA.
The mRNA profile from ABA-treated, prematurely senescing petals visualized
by differential display shows a high degree of similarity to the mRNA
profile of naturally senescing petals 18 h later. In addition, endogenous
ABA increases before flower opening and continues to increase during petal
senescence. An osmotic stress by sorbitol increases endogenous levels of
ABA and upregulates the same parameters of senescence as those occurring
during natural senescence and after application of ABA. The mRNA profile
from sorbitol-treated, prematurely senescing petals, but somewhat less
similarity to mRNA from ABA-treated petals. The possibility is discussed
that ABA is a constituent of the signal transduction chain leading to
programmed cell death of daylily petals. 相似文献