共查询到20条相似文献,搜索用时 31 毫秒
1.
Genetic evidence that small maf proteins are essential for the activation of antioxidant response element-dependent genes 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Katsuoka F Motohashi H Ishii T Aburatani H Engel JD Yamamoto M 《Molecular and cellular biology》2005,25(18):8044-8051
While small Maf proteins have been suggested to be essential for the Nrf2-mediated activation of antioxidant response element (ARE)-dependent genes, the extent of their requirement remains to be fully documented. To address this issue, we generated mafG::mafF double-mutant mice possessing MafK as the single available small Maf. Induction of the NAD(P)H:quinone oxidoreductase 1 (NQO1) gene was significantly impaired in double-mutant mice treated with butylated hydroxyanisole, while other ARE-dependent genes were less affected. Similarly, in a keap1-null background, where many of the ARE-dependent genes are constitutively activated in an Nrf2-dependent manner, only a subset of ARE-dependent genes, including NQO1, were sensitive to a simultaneous deficiency in MafG and MafF. Examination of single and double small maf mutant cells revealed that MafK also contributes to the induction of ARE-dependent genes. To obtain decisive evidence, we established mafG::mafK::mafF triple-mutant fibroblasts that completely lack small Mafs and turned out to be highly susceptible to oxidative stress. We found that induction in response to diethyl maleate was abolished in a wider range of ARE-dependent genes in the triple-mutant cells. These data explicitly demonstrate that small Mafs play critical roles in the inducible expression of a significant portion of ARE-dependent genes. 相似文献
2.
MafT, a new member of the small Maf protein family in zebrafish 总被引:3,自引:0,他引:3
Takagi Y Kobayashi M Li L Suzuki T Nishikawa K Yamamoto M 《Biochemical and biophysical research communications》2004,320(1):62-69
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to identify and characterize small Maf proteins in zebrafish to elucidate their conservation and diversity in the fish kingdom. We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. In addition, a novel type of small Maf protein MafT was identified. MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. These results indicated that MafT is a new member of small Maf proteins and involved in the Nrf2-dependent gene regulation in cellular defense system. 相似文献
3.
Yamazaki H Katsuoka F Motohashi H Engel JD Yamamoto M 《Molecular and cellular biology》2012,32(4):808-816
Embryogenesis is a period during which cells are exposed to dynamic changes of various intracellular and extracellular stresses. Oxidative stress response genes are regulated by heterodimers composed of Cap'n'Collar (CNC) and small Maf proteins (small Mafs) that bind to antioxidant response elements (ARE). Whereas CNC factors have been shown to contribute to the expression of ARE-dependent cytoprotective genes during embryogenesis, the specific contribution of small Maf proteins to such gene regulation remains to be fully examined. To delineate the small Maf function in vivo, in this study we examined mice lacking all three small Mafs (MafF, MafG, and MafK). The small Maf triple-knockout mice developed normally until embryonic day 9.5 (E9.5). Thereafter, however, the triple-knockout embryos showed severe growth retardation and liver hypoplasia, and the embryos died around E13.5. ARE-dependent cytoprotective genes were expressed normally in E10.5 triple-knockout embryos, but the expression was significantly reduced in the livers of E13.5 mutant embryos. Importantly, the embryonic lethality could be completely rescued by transgenic expression of exogenous MafG under MafG gene regulatory control. These results thus demonstrate that small Maf proteins are indispensable for embryonic development after E9.5, especially for liver development, but early embryonic development does not require small Mafs. 相似文献
4.
5.
6.
7.
8.
9.
10.
Small Maf compound mutants display central nervous system neuronal degeneration,aberrant transcription,and Bach protein mislocalization coincident with myoclonus and abnormal startle response
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Katsuoka F Motohashi H Tamagawa Y Kure S Igarashi K Engel JD Yamamoto M 《Molecular and cellular biology》2003,23(4):1163-1174
11.
Multiple mechanisms and functions of maf transcription factors in the regulation of tissue-specific genes 总被引:2,自引:0,他引:2
Kataoka K 《Journal of biochemistry》2007,141(6):775-781
12.
13.
Cadmium induces nuclear export of Bach1, a transcriptional repressor of heme oxygenase-1 gene 总被引:5,自引:0,他引:5
Suzuki H Tashiro S Sun J Doi H Satomi S Igarashi K 《The Journal of biological chemistry》2003,278(49):49246-49253
14.
15.
16.
17.
18.
19.