共查询到20条相似文献,搜索用时 78 毫秒
1.
Van Balkom Roland H.H.; Zhan Wen-Zhi; Prakash Y. S.; Dekhuijzen P. N.Richard; Sieck Gary C. 《Journal of applied physiology》1997,83(4):1062-1067
Van Balkom, Roland H. H., Wen-Zhi Zhan, Y. S. Prakash, P. N. Richard Dekhuijzen, and Gary C. Sieck. Corticosteroid effects on isotonic contractile properties of rat diaphragm muscle. J. Appl. Physiol. 83(4):1062-1067, 1997.The effects of corticosteroids (CS) on diaphragmmuscle (Diam) fiber morphologyand contractile properties were evaluated in three groups of rats:controls (Ctl), surgical sham and weight-matched controls (Sham), andCS-treated (6 mg · kg1 · day1prednisolone at 2.5 ml/h for 3 wk). In the CS-treatedDiam, there was a selectiveatrophy of type IIx and IIb fibers, compared with a generalized atrophyof all fibers in the Sham group. Maximum isometric force was reduced by20% in the CS group compared with both Ctl and Sham. Maximumshortening velocity in the CS Diam was slowed by ~20% compared with Ctl and Sham. Peak power output ofthe CS Diam was only 60% of Ctland 70% of Sham. Endurance to repeated isotonic contractions improvedin the CS-treated Diam comparedwith Ctl. We conclude that the atrophy of type IIx and IIb fibers inthe Diam can only partiallyaccount for the CS-induced changes in isotonic contractile properties.Other factors such as reduced myofibrillar density or alteredcross-bridge cycling kinetics are also likely to contribute to theeffects of CS treatment. 相似文献
2.
Roy Roland R.; Talmadge Robert J.; Fox Kenneth; Lee Michael; Ishihara Aki; Edgerton V. Reggie 《Journal of applied physiology》1997,83(1):280-290
Roy, Roland R., Robert J. Talmadge, Kenneth Fox, MichaelLee, Aki Ishihara, and V. Reggie Edgerton. Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers.J. Appl. Physiol. 83(1): 280-290, 1997.The effects of 1 and 10 wk of functional overload (FO) of therat plantaris with (FOTr) andwithout daily endurance treadmill training on its myosin heavy chain(MHC) composition were studied. After 1 and 10 wk of FO, plantaris masswas 22 and 56% greater in FO and 37 and 94% greater, respectively, inFOTr rats compared withage-matched controls. At 1 wk, pure type I and pure type IIa MHC fiberswere hypertrophied in FO (39 and 44%) andFOTr (70 and 87%) rats. By 10 wkall fiber types comprising >5% of the fibers sampled showed ahypertrophic response in both FO groups. One week of FO increased thepercentage of hybrid (containing both type I and type IIa MHC) fibersand of fibers containing embryonic MHC. By 10 wk, the percentage ofpure type I MHC fibers was ~40% in both FO groups compared with 15%in controls, and the percentage of fibers containing embryonic MHC wassimilar to that in controls. Sodium dodecyl sulfate-polyacrylamide gelelectrophoresis analyses showed an increase in type I MHC and adecrease in type IIb MHC in both FO groups at 10 wk, whereas littlechange was observed at 1 wk. These data are consistent with hypertrophyand transformation from faster to slower MHC isoforms in chronicallyoverloaded muscles. The additional overload imposed by daily endurancetreadmill training employed in this study (1.6 km/day; 10% incline)results in a larger hypertrophic response but appears to have a minimaleffect on the MHC adaptations. 相似文献
3.
Zhan Wen-Zhi; Watchko Jon F.; Prakash Y. S.; Sieck Gary C. 《Journal of applied physiology》1998,84(4):1260-1268
Postnatal transitions in myosin heavy chain (MHC) isoformexpression were found to be associated with changes in both isometric and isotonic contractile properties of rat diaphragm muscle(Diam). Expression of MHCneo predominated inneonatal Diam fibers but was usually coexpressed withMHCslow or MHC2A isoforms. Expression ofMHCneo disappeared by day 28. Expression ofMHC2X and MHC2B emerged at day 14 andincreased thereafter. Associated with these MHC transitions in theDiam, maximum isometric tetanic force (Po), maximum shortening velocity, and maximum power output progressively increased during early postnatal development. Maximum power output ofthe Diam occurred at ~40% Po at days0 and 7 and at ~30% Po in older animals.Susceptibility to isometric and isotonic fatigue, defined as a declinein force and power output during repetitive activation, respectively,increased with maturation. Isotonic endurance time, defined as the timefor maximum power output to decline to zero, progressively decreasedwith maturation. In contrast, isometric endurance time, defined as thetime for force to decline to 30-40% Po, remained>300 s until after day 28. We speculate that with thepostnatal transition to MHC2X and MHC2Bexpression energy requirements for contraction increase, especiallyduring isotonic shortening, leading to a greater imbalance betweenenergy supply and demand. 相似文献
4.
Contractile function of single muscle fibers after hindlimb unweighting in aged rats 总被引:1,自引:0,他引:1
Thompson, L. V., and J. A. Shoeman. Contractilefunction of single muscle fibers after hindlimb unweighting in aged rats. J. Appl. Physiol. 84(1):229-235, 1998.This investigation determined how muscle atrophyproduced by hindlimb unweighting (HU) alters the contractile functionof single muscle fibers from older animals (30 mo). After 1 wk of HU,small bundles of fibers were isolated from the soleus muscles and thedeep region of the lateral head of the gastrocnemius muscles. Singleglycerinated fibers were suspended between a motor lever and forcetransducer, functional properties were studied, and the myosin heavychain (MHC) composition was determined electrophoretically. After HU, the diameter of type I MHC fibers of the soleus declined (88 ± 2 vs. 80 ± 4 µm) and reductions were observed in peak active force (47 ± 3 vs. 28 ± 3 mg) and peak specific tension(Po; 80 ± 5 vs. 56 ± 5 kN/m2). The maximal unloadedshortening velocity increased. The type I MHC fibers from thegastrocnemius showed reductions in diameter (14%), peak active force(41%), and Po (24%), whereas thetype IIa MHC fibers showed reductions in peak active force andPo. Thus 1 wk ofinactivity has a significant effect on the force-generating capacity ofsingle skeletal muscle fibers from older animals in a fibertype-specific manner (type I MHC > type IIa MHC > type I-IIa MHC).The decline in the functional properties of single skeletal musclefibers in the older animals appears to be more pronounced than what hasbeen reported in younger animal populations. 相似文献
5.
Novel transitions in MHC isoforms: separate and combined effects of thyroid hormone and mechanical unloading 总被引:1,自引:0,他引:1
Caiozzo Vincent J.; Baker Michael J.; Baldwin Kenneth M. 《Journal of applied physiology》1998,85(6):2237-2248
Single-fiber(n = 3,818 fibers) electrophoreticanalyses were used to delineate the separate and combined effects ofhyperthyroidism (T3) andhindlimb suspension (HS) on the myosin heavy chain (MHC) isoformcomposition (1-, 2-, and 4-wk time points) of the rat soleus muscle.The key findings of this study are as follows. First,T3 and HS both altered thedistribution of MHC isoforms at the single-fiber level; however, thepopulations of fibers produced by these two interventions were clearlydifferent from one another. Second,T3 + HS rapidly converted thesoleus into a fast muscle, producing large increases in the relativecontents of the fast type IIx and IIb MHC isoforms which were primarily expressed in several populations of hybrid fibers (e.g., types I/IIa/IIx, I/IIx/IIb, I/IIa/IIx/IIb). Finally,T3 + HS produced uniquepopulations of hybrid fibers that did not adhere to the IIIaIIxIIb sequential scheme of MHC plasticity. Collectively, the findings of this study demonstrate that the intervention of T3 + HS is a powerful model formanipulating and studying MHC isoform plasticity in slow skeletal muscle. 相似文献
6.
Van Der Heijden H. F.M.; Zhan W. Z.; Prakash Y. S.; Dekhuijzen P. N.R.; Sieck G. C. 《Journal of applied physiology》1998,85(2):525-529
The effects of the2-adrenoceptor agonistsalbutamol (Slb) on isometric and isotonic contractile properties ofthe rat diaphragm muscle(Diamus) were examined. Aloading dose of 25 µg/kg Slb was administered intracardially beforeDiamus excision to ensure adequatediffusion. Studies were then performed with 0.05 µMSlb in the in vitro tissue chamber. cAMP levels were determined by radioimmunoassay. Compared with controls (Ctl), cAMP levels were elevated after Slb treatment. In Slb-treated rats, isometric twitch andmaximum tetanic force were increased by ~40 and ~20%,respectively. Maximum shortening velocity increased by ~15% afterSlb treatment, and maximum power output increased by ~25%. Duringrepeated isotonic activation, the rate of fatigue was faster in theSlb-treated Diamus, but bothSlb-treated and Ctl Diamusfatigued to the same maximum power output. Still, endurance time duringrepetitive isotonic contractions was ~10% shorter in the Slb-treatedDiamus. These results areconsistent with the hypothesis that -adrenoceptor stimulation by Slbenhances Diamus contractility andthat these effects of Slb are likely mediated, at least in part, byelevated cAMP. 相似文献
7.
Interaction of hyperthyroidism and hindlimb suspension on skeletal myosin heavy chain expression 总被引:1,自引:0,他引:1
Haddad F.; Qin A. X.; Zeng M.; McCue S. A.; Baldwin K. M. 《Journal of applied physiology》1998,85(6):2227-2236
We examined the novel interaction ofhyperthyroidism and hindlimb suspension on the pattern of myosin heavychain (MHC) expression (mRNA and protein) in skeletal muscles. FemaleSprague-Dawley rats were assigned to four groups:1) normal control (Con);2) thyroid hormone treated[150 µg 3,5,3'-triiodothyronine(T3) · kg1 · day1](T3);3) hindlimb suspension (HS); or4)T3-treated and HS(T3 + HS). Results show for thefirst time the novel observation that the combinationT3 + HS induces a rapid andsustained, marked (80-90%) downregulation of type I MHC geneexpression that is mirrored temporally by concomitant markedupregulation of type IIb MHC gene expression, as evidenced by the denovo synthesis of type IIb MHC protein in the soleus. The fast type IIxMHC isoform showed a differential response among the experimentalgroups, generally increasing with the separate and combined treatments in both the soleus and vastus intermedius muscles while decreasing inthe plantaris muscles. The fast type IIa MHC was the least responsiveto suspension of the MHCs and reflected its greatest responsiveness toT3 treatment while also undergoingdifferential adaptations in slow vs. fast muscle (increases vs.decreases, respectively). These results confirm previous findings thatall four adult MHC genes are sensitive toT3 and suspension in amuscle-specific manner. In addition, we show thatT3 + HS can interactsynergistically to create novel adaptations in MHC expression thatcould not be observed when each factor was imposed separately. 相似文献
8.
In the cat diaphragm, fiber capillarity, cross-sectional area, and succinate dehydrogenase (SDH) activity were measured across the first 6 wk of postnatal development. Fibers were classified as type I, IIa, IIb, or IIc on the basis of staining for myofibrillar adenosinetriphosphatase (ATPase). Capillaries were identified in sections stained for ATPase at pH 4.2. Fiber cross-sectional areas and SDH activities were quantified using an image-processing system. During postnatal development, the proportions of type I fibers increased while type II fibers decreased. At birth, all type II fibers were IIc. From the 1st to the 2nd postnatal wk, the proportion of type IIc fibers decreased while the numbers of IIa and IIb increased. Thereafter the proportion of type IIb fibers continued to increase while the number of IIa steadily declined. At birth, capillarity, cross-sectional areas, and SDH activities of type I and II fibers were low compared with other postnatal age groups. Fiber cross-sectional areas increased progressively with age. The number of capillaries surrounding type I and II fibers increased markedly by the 2nd wk and then continued to increase at a slower rate. The number of capillaries per fiber area reached a peak by the 2nd wk and then declined as fiber cross-sectional area increased. Postnatal changes in capillarity depended on fiber type, being greatest in IIb. SDH activities of type I and II fibers were initially low during the first 2 postnatal wk and then peaked by the 3rd wk. After the 6th wk, fiber SDH activities decreased to adult values. Among the type II fibers, IIb showed the greatest change in SDH activity during early postnatal development. 相似文献
9.
Widrick JJ 《American journal of physiology. Cell physiology》2002,282(4):C647-C653
Chemically skinned muscle fibers,prepared from the rat medial gastrocnemius and soleus, were subjectedto four sequential slack tests in Ca2+-activating solutionscontaining 0, 15, 30, and 0 mM added Pi. Pi (15 and 30 mM) had no effect on the unloaded shortening velocity (Vo) of fibers expressing type IIb myosin heavychain (MHC). For fibers expressing type I MHC, 15 mM Pi didnot alter Vo, whereas 30 mM Pireduced Vo to 81 ± 1% of the original 0 mM Pi value. This effect was readily reversible whenPi was lowered back to 0 mM. These results are notcompatible with current cross-bridge models, developed exclusively fromdata obtained from fast fibers, in which Vo isindependent of Pi. The response of the type I fibers at 30 mM Pi is most likely the result of increased internal drag opposing fiber shortening resulting from fiber type-specific effects ofPi on cross bridges, the thin filament, or therate-limiting step of the cross-bridge cycle. 相似文献
10.
Mitochondrial respiratory rates and regulation by phosphate acceptors were studied on permeabilized fiber bundles differing in their myosin heavy chain profiles. The acceptor control ratio, an indicator of oxidation to phosphorylation coupling, and mitochondrial Km for ADP were the highest in type I, intermediate in mixed IIa/IIx and the lowest in IIx and predominantly IIb fiber bundles. A functional coupling between mitochondrial creatine kinase and oxidative phosphorylation occurred in type I and IIa/IIx fiber bundles, exclusively. Our study suggests that mitochondrial functioning in fast IIa fibers is closer to that of the slow/I than fast IIx or IIb fibers. (Mol Cell Biochem 276: 15–20, 2005) 相似文献
11.
Myosin heavy chain mRNA transform to faster isoforms in immobilized skeletal muscle: a quantitative PCR study 总被引:4,自引:0,他引:4
Jankala Heidi; Harjola Veli-Pekka; Petersen Niels Erik; Harkonen Matti 《Journal of applied physiology》1997,82(3):977-982
Jänkälä, Heidi, Veli-Pekka Harjola, NielsErik Petersen, and Matti Härkönen. Myosin heavy chainmRNA transform to faster isoforms in immobilized skeletal muscle: aquantitative PCR study. J. Appl.Physiol. 82(3): 977-982, 1997.A quantitative polymerase chain reaction (PCR) method was used to measure the quantities of type I, IIa, IIx, and IIb myosin heavy chain (MHC) mRNAin total RNA preparations of the soleus, gastrocnemius, and plantarismuscles of normal and hindlimb-immobilized rats. Type IIx and even typeIIb MHC mRNA were demonstrated at extremely low levels in normalsoleus, 2.1 ± 0.4 × 105and 5.0 ± 0.2 × 105molecules of mRNA per microgram total RNA, respectively. Immobilization for 1 wk significantly altered the gene expression of MHC isoforms. Insoleus, both type IIx and IIb MHC genes became significantly upregulated, 24-fold (P < 0.005) and 2.6-fold (P < 0.05),respectively. In gastrocnemius, the level of type IIa MHC mRNAdecreased by 51% (P < 0.01) and thelevel of type IIx MHC mRNA increased by 140%(P < 0.05). In plantaris, the levelof type IIa MHC mRNA decreased by 58%(P < 0.005). In conclusion,immobilization changed the MHC mRNA profile in three different types ofskeletal muscle toward faster isoforms. The quantitative results permitreliable evaluation of changes in mRNA levels. 相似文献
12.
Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers 总被引:4,自引:0,他引:4
Talmadge Robert J.; Roy Roland R.; Edgerton V. Reggie 《Journal of applied physiology》1996,81(6):2540-2546
Talmadge, Robert J., Roland R. Roy, and V. Reggie Edgerton.Distribution of myosin heavy chain isoforms in non-weight-bearing rat soleus muscle fibers. J. Appl.Physiol. 81(6): 2540-2546, 1996.The effects of14 days of spaceflight (SF) or hindlimb suspension (HS) (Cosmos 2044)on myosin heavy chain (MHC) isoform content of the rat soleus muscleand single muscle fibers were determined. On the basis ofelectrophoretic analyses, there was a de novo synthesis of type IIx MHCbut no change in either type I or IIa MHC isoform proportions aftereither SF or HS compared with controls. The percentage of fiberscontaining only type I MHC decreased by 26 and 23%, and the percentageof fibers with multiple MHCs increased from 6% in controls to 32% inHS and 34% in SF rats. Type IIx MHC was always found in combinationwith another MHC or combination of MHCs; i.e., no fibers contained typeIIx MHC exclusively. These data suggest that the expression of thenormal complement of MHC isoforms in the adult rat soleus muscle isdependent, in part, on normal weight bearing and that the absence ofweight bearing induces a shift toward type IIx MHC protein expression in the preexisting type I and IIa fibers of the soleus. 相似文献
13.
Haddad Fadia; Qin Anqi X.; Zeng Ming; McCue Sam A.; Baldwin Kenneth M. 《Journal of applied physiology》1998,84(6):2036-2041
This studytested the hypothesis that an isometric resistance-training programinduces upregulation of slow myosin heavy chain (MHC) expression in afast-twitch skeletal muscle. Thus we studied the effects of tworesistance-training programs on rodent medial gastrocnemius (MG) musclethat were designed to elicit repetitive isometric contractions(10-12 per set; 4 sets per session) of different duration (8 vs. 5 s) and activation frequency (100 vs. 60 Hz) per contraction during eachtraining session (total of 6 and 12 sessions). Results showed that bothtraining paradigms produced significant increases in muscle weight(~11-13%) after completion of training(P < 0.05). Significanttransformations in MHC expression occurred and involved specifically adecrease in the relative expression of the fast type IIb MHC andconcomitant increased expression of the fast type IIx MHC.These adaptations were observed in both the "white" and"red" regions of the MG, and they occurred at both the mRNA andprotein levels. These adaptations were detected after onlysix training sessions. Neither of the training programs produced anychange in the relative expression of either the slow type I MHC or themoderately fast type IIa MHC, which can be upregulated in the red MG bychronic functional overload. These findings show that theisometric protocols used in this investigation were not sufficient toinduce the hypothesized changes in the myosin heavy chain isoformexpression in rodent skeletal muscle. 相似文献
14.
Frank Andreas O.; Chuong C. J.Charles; Johnson Robert L. 《Journal of applied physiology》1997,82(6):2036-2044
Frank, Andreas O., C. J. Charles Chuong, and Robert L. Johnson. A finite-element model of oxygen diffusion in thepulmonary capillaries. J. Appl.Physiol. 82(6): 2036-2044, 1997.We determined the overall pulmonary diffusing capacity(DL) and the diffusing capacities of the alveolar membrane (Dm) and the red blood cell (RBC)segments (De) of the diffusional pathway forO2 by using a two-dimensionalfinite-element model developed to represent the sheet-flowcharacteristics of pulmonary capillaries. An axisymmetric model wasalso considered to assess the effect of geometric configuration. Results showed the membrane segment contributing the major resistance, with the RBC segment resistance increasing asO2 saturation(SO2) rises during the RBC transit:RBC contributed 7% of the total resistance at the capillary inlet (SO2 = 75%) and 30% toward thecapillary end (SO2 = 95%) for a 45%hematocrit (Hct). Both Dm and DLincreased as the Hct increased but began approaching a plateau near anHct of 35%, due to competition between RBCs forO2 influx. Both Dm andDL were found to be relatively insensitive (2~4%) to changes in plasma protein concentration (28~45%). Axisymmetric results showed similar trends for all Hct andprotein concentrations but consistently overestimated the diffusingcapacities (~2.2 times), primarily because of an exaggerated air-tissue barrier surface area. The two-dimensional model correlated reasonably well with experimental data and can better represent theO2 uptake of the pulmonarycapillary bed. 相似文献
15.
Sanford Levine Chris Gregory Taitan Nguyen Joseph Shrager Larry Kaiser Neal Rubinstein Gary Dudley 《Journal of applied physiology》2002,92(3):1205-1213
To assess the effect of severe chronic obstructive pulmonary disease (COPD) on the ability of human diaphragmatic myofibers to aerobically generate ATP relative to ATP utilization, we obtained biopsy specimens of the costal diaphragm from seven patients with severe COPD (mean +/- SE; age 56 +/- 1 yr; forced expiratory volume in 1 s 23 +/- 2% predicted; residual volume 267 +/- 30% predicted) and seven age-matched control subjects. We categorized all fibers in these biopsies by using standard techniques, and we carried out the following quantitative histochemical measurements by microdensitometry: 1) succinate dehydrogenase (SDH) activity as an indicator of mitochondrial oxidative capacity and 2) calcium-activated myosin ATPase (mATPase) activity, the ATPase that represents a major portion of ATP consumption by contracting muscle. We noted the following: 1) COPD diaphragms had a larger proportion of type I fibers, a lesser proportion of type IIax fibers, and the same proportion of type IIa fibers as controls. 2) SDH activities of each of the fiber types were higher in COPD than control diaphragms (P < 0.0001); the mean increases (expressed as percent of control values) in types I, IIa, and IIax were 84, 114, and 130%, respectively. 3) COPD elicited no change in mATPase activity of type I and IIa fibers, but mATPase decreased in type IIax fibers (P = 0.02). 4) Mitochondrial oxidative capacity relative to ATP demand (i.e., SDH/mATPase) was higher (P = 0.03) in each of the fiber types in COPD diaphragms than in controls. These results demonstrate that severe COPD elicits an increase in aerobic ATP generating capacity relative to ATP utilization in all diaphragmatic fiber types as well as the previously described fast-to-slow fiber type transformation (Levine S, Kaiser L, Leferovich J, and Tikunov B, N Engl J Med 337: 1799-1806, 1997). 相似文献
16.
Latzka William A.; Sawka Michael N.; Montain Scott J.; Skrinar Gary S.; Fielding Roger A.; Matott Ralph P.; Pandolf Kent B. 《Journal of applied physiology》1997,83(3):860-866
Latzka, William A., Michael N. Sawka, Scott J. Montain, GaryS. Skrinar, Roger A. Fielding, Ralph P. Matott, and Kent B. Pandolf.Hyperhydration: thermoregulatory effects during compensable exercise-heat stress. J. Appl.Physiol. 83(3): 860-866, 1997.This studyexamined the effects of hyperhydration on thermoregulatory responsesduring compensable exercise-heat stress. The general approach was todetermine whether 1-h preexercise hyperhydration [29.1 ml/kg leanbody mass; with or without glycerol (1.2 g/kg lean body mass)]would improve sweating responses and reduce core temperature duringexercise. During these experiments, the evaporative heat loss required(Ereq = 293 W/m2) to maintain steady-statecore temperature was less than the maximal capacity(Emax = 462 W/m2) of the climate forevaporative heat loss(Ereq/Emax = 63%). Eight heat-acclimated men completed five trials: euhydration, glycerol hyperhydration, and water hyperhydration both with and withoutrehydration (replace sweat loss during exercise). During exercise inthe heat (35°C, 45% relative humidity), there was no differencebetween hyperhydration methods for increasing total body water (~1.5liters). Compared with euhydration, hyperhydration did not alter coretemperature, skin temperature, whole body sweating rate, local sweatingrate, sweating threshold temperature, sweating sensitivity, or heartrate responses. Similarly, no difference was found between water andglycerol hyperhydration for these physiological responses. These datademonstrate that hyperhydration provides no thermoregulatory advantageover the maintenance of euhydration during compensable exercise-heatstress. 相似文献
17.
In mammals the type IIb Na/Pi-cotransporter is expressed in various tissues such as intestine, brain, lung and testis. The type IIb cotransporter shows 51% homology with the renal type IIa Na/Pi-cotransporter, for which a detailed model of the secondary structure has emerged based on recent structure/function studies. To make the type IIb Na/Pi-cotransporter available for future structural studies, we have expressed this cotransporter in Sf9 cells. Sf9 cells were infected with recombinant baculovirus containing 6His NaPi-IIb. Infected cells expressed a polypeptide of ~90 kDa, corresponding to a partially glycosylated form of the type IIb cotransporter. Transport studies demonstrated that the type IIb protein expressed in Sf9 cells mediates transport of phosphate in a Na-dependent manner with similar kinetic characteristics (apparent K
ms for sodium and phosphate and pH dependence) as previously described. Solubilization experiments demonstrated that, in contrast to the type IIa cotransporter, the type IIb can be solubilized by nonionic detergents and that solubilized type IIb Na/Pi-cotransporter can be purified by Ni-NTA chromatography. 相似文献
18.
Schepkin, V. D., I. O. Choy, and T. F. Budinger. Sodiumalterations in isolated rat heart during cardioplegic arrest. J. Appl. Physiol. 81(6):2696-2702, 1996.Triple-quantum-filtered (TQF) Na nuclearmagnetic resonance (NMR) without chemical shift reagent is used toinvestigate Na derangement in isolated crystalloid perfused rat heartsduring St. Thomas cardioplegic (CP) arrest. Theextracellular Na contribution to the NMR TQF signal of a rat heart isfound to be 73 ± 5%, as determined by wash-out experiments atdifferent moments of ischemia and reperfusion. With the use of thiscontribution factor, the estimated intracellular Na([Na+]i)TQF signal is 222 ± 13% of preischemic level after 40 min of CParrest and 30 min of reperfusion, and the heart rate pressure productrecovery is 71 ± 8%. These parameters aresignificantly better than for stop-flow ischemia: 340 ± 20% and 6 ± 3%, respectively. At 37°C, the initial delay of 15 min in[Na+]igrowth occurs during CP arrest along with reduced growth later (~4.0%/min) in comparison with stop-flow ischemia (~6.7%/min). The hypothermia (21°C, 40 min) for the stop-flow ischemia and CPdramatically decreases the[Na+]igain with the highest heart recovery for CP (~100%). These studiesconfirm the enhanced sensitivity of TQF NMR to[Na+]iand demonstrate the potential of NMR without chemical shift reagent tomonitor[Na+]iderangements. 相似文献
19.
Velocity, force, power, and Ca2+ sensitivity of fast and slow monkey skeletal muscle fibers 总被引:1,自引:0,他引:1
Fitts Robert H.; Bodine Sue C.; Romatowski Janell G.; Widrick Jeffrey J. 《Journal of applied physiology》1998,84(5):1776-1787
In this study,we determined the contractile properties of single chemically skinnedfibers prepared from the medial gastrocnemius (MG) and soleus (Sol)muscles of adult male rhesus monkeys and assessed the effects of thespaceflight living facility known as the experiment support primatefacility (ESOP). Muscle biopsies were obtained 4 wk before andimmediately after an 18-day ESOP sit, and fiber type was determined byimmunohistochemical techniques. The MG slow type I fiber wassignificantly smaller than the MG type II, Sol type I, and Sol type IIfibers. The ESOP sit caused a significant reduction in the diameter oftype I and type I/II (hybrid) fibers of Sol and MG type II and hybridfibers but no shift in fiber type distribution. Single-fiber peak force(mN and kN/m2) was similarbetween fiber types and was not significantly different from valuespreviously reported for other species. The ESOP sit significantlyreduced the force (mN) of Sol type I and MG type II fibers. Thisdecline was entirely explained by the atrophy of these fiber typesbecause the force per cross-sectional area (kN/m2) was not altered. Peakpower of Sol and MG fast type II fiber was 5 and 8.5 times that of slowtype I fiber, respectively. The ESOP sit reduced peak power by 25 and18% in Sol type I and MG type II fibers, respectively, and, for theformer fiber type, shifted the force-pCa relationship to the right,increasing the Ca2+ activationthreshold and the free Ca2+concentration, eliciting half-maximal activation. The ESOP sit had noeffect on the maximal shortening velocity(Vo) of anyfiber type. Vo ofthe hybrid fibers was only slightly higher than that of slow type Ifibers. This result supports the hypothesis that in hybrid fibers theslow myosin heavy chain would be expected to have a disproportionatelygreater influence onVo. 相似文献
20.
Nociceptive atrophy of the rat soleus muscle induced by bone fracture: a morphometric study 总被引:1,自引:0,他引:1
Zachaová, Gisela, HelenaKnotková-Urbancová, Pavel Hník, andTomá Soukup. Nociceptive atrophy of the rat soleus muscle induced by bone fracture: a morphometric study.J. Appl. Physiol. 82(2): 552-557, 1997.Reflex atrophy of the soleus muscle induced by ipsilateralmetatarsal bone fracture in Sagatal-anesthetized adult male rats wasstudied by using two-dimensional stereological methods 7 days after theoperation. When compared with contralateral solei, the wet weight ofthe experimental soleus muscles was decreased by ~24% and the areaof the entire muscle section by ~29%. In atrophied solei, the numberof type 1 fibers was lower by ~8%, resulting in lower total numberof fibers (by ~6%). This indicates that slow motor units might bemore sensitive to nociceptive stimulation. However, with respect to thefiber area, the reflex atrophy induced by metatarsal bone fracture inthe rat soleus muscle resembles simple atrophy after 7 days, as themean muscle fiber area was decreased by ~26% with no significantdifference between atrophy in type 1 and type 2a fibers (by 27.3 and23.0%, respectively). 相似文献