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1.
植物体内水分长距离运输的生理生态学机制   总被引:8,自引:0,他引:8       下载免费PDF全文
植物体内长距离水分运输是植物生理生态学研究中的一个重要问题,长期为植物生理学家和生理生态学家所关注。木质部探针技术的问世,掀起了近年来植物生理学界最为激烈的一场争论。提出了已经有100多年,风行40年的内聚力-张力(Cohesion-Tension, C-T)学说受到质疑。随后维护派和质疑派围绕木质部探针技术、压力室技术(C-T理论的主要支撑实验技术)的可靠性展开辩论。进一步从物理学原理和各种实验上就C-T理论的3个支柱(木质部导管或管胞中巨大的张力、沿树高的压力梯度、连续水柱)进行争论。这场争论似暂告一段落,C-T理论没有被推翻,但仍留有问题期待以后的研究。  相似文献   

2.
Simultaneous measurements were made with the xylem pressure probe on exposed, transpiring leaves and with the Scholander pressure chamber on both transpiring and covered, non-transpiring leaves of sugarcane and maize plants. Xylem tensions inferred from pressure chamber balancing pressures on non-transpiring leaves were similar to those measured directly with the xylem pressure probe in transpiring leaves. However, tensions inferred with the pressure chamber on transpiring leaves that were placed in plastics bags just prior to excision were up to 0.6 MPa greater than those measured concurrently with the xylem pressure probe. These findings suggest that relatively large differences in water potential between the xylem and bulk leaf tissue can exist during periods of rapid transpiration, and they confirm that the balance pressure of an excised, previously transpiring leaf is only a measure of the bulk average equilibrium leaf water potential and not of the true xylem pressure that existed prior to excision.Key words: Cohesion-Tension theory, xylem pressure probe, pressure chamber, xylem tension.   相似文献   

3.
The essentials of direct xylem pressure measurement   总被引:5,自引:0,他引:5  
This paper discusses the essentials of the oil‐filled pressure probe technique in the measurement of negative xylem pressures, focusing in particular on the technique and physics underlying our recent, successful experiment which has rekindled the debate on the validity of the Cohesion–Tension theory. We illustrate a number of general problems associated with the cell pressure probe and xylem pressure probe techniques, and propose appropriate criteria for micropipette construction. We enumerate factors dealing with the cavitation problem and suggest methods for eliminating air seeds in the system. We introduce reliable criteria for the successful measurement of xylem pressure, and emphasize the importance of the probe pressure relaxation test. Several problems regarding the controversy over the Cohesion–Tension theory are also discussed. We discuss the correlation between xylem pressure and the transpiration rate, the existence of absolute negative xylem pressure in intact plants, the most negative values of xylem pressure measured by the pressure probe, the agreement between the pressure probe and pressure bomb techniques, and the vulnerability to cavitation (tensile strength) of pressure probes.  相似文献   

4.
Hydraulic and osmotic properties of spruce roots   总被引:9,自引:6,他引:3  
Hydraulic and osmotic properties of roots of 2-year-old Norwayspruce seedlings (Plcea abiea (L.) Karst) were investigatedusing different techniques (steady flow, pressure probe, andstop flow technique). Root pressures were measured using theroot pressure probe. Compared to roots of herbaceous plantsor deciduous trees, excised root systems of spruce did not developappreciable root pressure (-0.001 to 0.004 MPa or -10 to 40cm of water column). When hydrostatic pressure gradients wereused to drive water flows across the roots, hydraulic conductivities(Lpr) were determined in two types of experiments: (i) rootpressure relaxations (using the root pressure probe) and (ii)steady flow experiments (pneumatic pressures applied to theroot system or xylem or partial vacuum applied to the xylem).Root Lpr ranged between 0.2 and 810–8m s–1 MPa–1(on average) depending on the conditions. In steady flow experiments,Lpr depended on the pressure applied (or on the flow acrossthe roots) and equalled (0.190.12) to (1.21.7)10–8m s–1 MPa–1 at pressures between 0.2 and 0.4 MPaand (1.51.3)10–8 m s–1 MPa–1 at appliedpressures between 0.8 and 1.0 MPa. When pressures or vacuumwere applied to the xylem, Lpr values were similar. The hydraulicconductivity measured during pressure relaxations (transientwater flows) was similar to that obtained at high pressures(and water flows). Although there was a considerable scatterin the data, there was a tendency of the hydraulic conductivityof the roots to decrease with increasing size of the root system.When osmotic gradients were used to drive water flows, Lpr valuesobtained with the root pressure probe were much smaller thanthose measured in the presence of hydrostatic gradients. Onaverage, a root Lpr=0.01710–8 was found for osmotic andLpr=6.410–8 m s–1 MPa–1 in correspondinghydrostatic experiments, i.e. the two values differed by a factorwhich was as large as 380. The same hydraulic conductivity asthat obtained in osmotic experiments using the pressure probewas obtained by the 'stop flow techniquel. In this technique,the suction created by an osmoticum applied to the root wasbalanced by a vacuum applied to the xylem. Lpr values of rootsystems did not change significantly when measured for up to5 d. In osmotic experiments with different solutes (Na2S04,K2S04, Ca(NO3)2, mannitol), no passive uptake of solutes couldbe detected, i.e. the solute permeability was very low whichwas different from earlier findings on roots of herbs. Reflectioncoefficients of spruce roots (O were low for solutes for whichplant cell membranes exhibit values of virtually unity (  相似文献   

5.
MILBURN  JOHN A. 《Annals of botany》1996,78(4):399-407
In recent years the cohesion theory has been attacked on thegrounds that direct measurements made with the pressure probeindicate that sap tensions are much less (maximum tension approx.0.7 MPa) than indicated by parallel measurements made with themore conventional methods: osmotic methods, pressure bomb, orpsychrometer. It has also been claimed that other direct methodsdo not support the cohesion theory. Thus a re-examination usingthe Renner technique indicated sap tensions of approx. 2.5 MPa.Also an independent method based on mercury penetrometry providesevidence that sap tensions of at least 2.0 MPa can be demonstrateddirectly implying, that serious limitations arise from the pressureprobe method itself. Without tensions exceeding 2.0 MPa mangroveswould be unable to extract fresh water for transpiration fromseawater. It is suggested that the pressure probe is susceptibleto bias because it investigates the least mature xylem conduitswhile they are still under varying degrees of turgor pressureand only partially interconnected with the main xylem system.This supposition is supported by claims that the xylem sap sampledby the probe contains significant concentrations of solutes.Additionally water, supplied by reverse osmosis from the sievetubes (‘Münch water’), is continually beingliberated in the vicinity of the outermost xylem vessels hydratingthem to an atypical degree which can explain several of thediscrepancies claimed. These results, which are supported bythe work of others, demonstrate that the challenges to the cohesiontheory for the ascent of sap are ill-founded. The release ofwater from the phloem can explain not only some discrepanciesclaimed by the cohesion challengers, but also explain the refillingof cavitated xylem conduits: a hitherto unsuspected role forthe phloem transport system. Cohesion theory; sap ascent; cavitation; pressure probe; xylem transport; vessel development; recycled water; reverse osmosis  相似文献   

6.
Wei C  Tyree MT  Steudle E 《Plant physiology》1999,121(4):1191-1205
The water relations of maize (Zea mays L. cv Helix) were documented in terms of hydraulic architecture and xylem pressure. A high-pressure flowmeter was used to characterize the hydraulic resistances of the root, stalk, and leaves. Xylem pressure measurements were made with a Scholander-Hammel pressure bomb and with a cell pressure probe. Evaporation rates were measured by gas exchange and by gravimetric measurements. Xylem pressure was altered by changing the light intensity, by controlling irrigation, or by gas pressure applied to the soil mass (using a root pressure bomb). Xylem pressure measured by the cell pressure probe and by the pressure bomb agreed over the entire measured range of 0 to −0.7 MPa. Experiments were consistent with the cohesion-tension theory. Xylem pressure changed rapidly and reversibly with changes in light intensity and root-bomb pressure. Increasing the root-bomb pressure increased the evaporation rate slightly when xylem pressure was negative and increased water flow rate through the shoots dramatically when xylem pressure was positive and guttation was observed. The hydraulic architecture model could predict all observed changes in water flow rate and xylem. We measured the cavitation threshold for oil- and water-filled pressure probes and provide some suggestions for improvement.  相似文献   

7.
Incisions used to offer radioisotopes and conditioning chemicalswere monitored for their effect on the transport system, usingmovement of 11C-photosynthate and 14C-sucrose to study phloemtransport in Helianthus and India ink to study xylem water movementin Heracleum. It was found that cuts made in air were more effectivethan cuts made under water, in part because both radioisotopesand conditioning chemicals penetrated more readily when cutswere made in air. Incisions made while the stem was under abackpressure, created by a pressure bomb, neutralized the releaseof pressure by the cut as seen in the movement of India inkin the xylem and biopotential changes as seen in vascular bundles. Key words: Phloem transport, Epidermal-wounding  相似文献   

8.
Excised roots of Plantago media L. were subjected to small hydrostaticpressure gradients. Pressure applied with a displacement pumpeasily extracted gas present in cortical lacunae consequenton the gas saturation deficit of the bathing solution. The temporarypressure treatment resulted in an irreversible decrease in theO2 diffusion rate through the root tissue and consequently inO2 deficient root cores. Therefore electrogenic pumps located at the symplast/xylem interface,i.e. in the centre of the root, were inhibited by pressure. The longitudinal distribution of the xylem pumps could be computed:in Plantago media they are located in the basal parts of theroot. The effect of pressure on the electrogenic xylem pumpsdemonstrated here should be taken into account when the processof ion transport from the root to the shoot is studied whilethe root is pressurized. (Received December 5, 1983; Accepted March 26, 1984)  相似文献   

9.
How Does Water Ascend in Tall Trees and Other Vascular Plants?   总被引:5,自引:1,他引:4  
Since the Cohesion Theory was first introduced, a series ofincompatible observations has appeared in the literature. Directmeasurements of xylem pressure in single vessels of higher plantsand tall trees by means of the xylem pressure probe techniqueindicate that xylem tension in the leaves of intact, transpiringplants is often much smaller than that predicted for transpiration-drivenwater ascent through continuous water columns. We conclude thatthe available evidence warrants a critical reappraisal of tension-drivenwater transport as the exclusive mechanism of long-distancewater transport in plants.Copyright 1995, 1999 Academic Press Cohesion Theory, higher plants, long-distance transport, trees, water ascent, xylem pressure probe  相似文献   

10.
Hydraulic properties of entire root systems and isolated rootsof three contrasting sugarcane clones were evaluated using transpiration-induceddifferences in hydrostatic pressure across intact root systems,root pressure-generated xylem sap exudation, and pressure-fluxrelationships. Regardless of the measurement technique employed,the clones were ranked in the same order on the basis of theirleaf area–specific total root system hydraulic conductance(Croot). All methods employed detected large developmental changesin Grootroot with maximum values occurring in plants with approximately02 m2 total leaf area. Genotypic ranking according to Groot,was reflected as a similar ranking according to root length-specifichydraulic conductance (L) of individual excised roots. Genotypicdifferences in Groot and L were consistent with anatomical characteristicsobserved in individual roots. Patterns of Groot, during soildrying and following re-irrigation suggested that the declinein Groot, observed during soil drying occurred within the rootsrather than at the soil–root interface and may have beencaused in part by xylem cavitation in the roots. Key words: Root hydraulic conductance, Saccharum spp, transpiration, root pressure, pressure-flux  相似文献   

11.
TYREE  M. T. 《Annals of botany》1981,47(5):547-559
A relationship has been derived mathematically between the bulkmodulus of elasticity of a leaf, E, measured by the pressurebomb technique, and the individual cellular bulk moduli, e,of the leaf tissue as measured by the pressure probe technique.A number of examples have been worked out that shows that Eunderestimates the mean value of e by less than 10 per centin most circumstances. Within 2 per cent, E equals the ‘mean’value of e weighted according to the water content change ofeach cell expressed as a fraction of the water content changeof the whole leaf when the water potential. , changes by a smallamount. An analysis of the sources of error involved in theevaluation of E by the pressure bomb technique reveals thatE can be calculated within an error of 10 per cent from valuesof and water loss, We; this error is about the same as theerror in e measured on individual cells by the pressure probetechnique. bulk elastic modulus, water potential isotherms, pressure bomb, pressure probe  相似文献   

12.
The vulnerability of xylem conduits to cavitation theoreticallydetermines the maximum flow rate of water through plants, andhence maximum transpiration (E), stomatal conductance (gs),and leaf area (A1. Field-grown Betula occidentalis with a favourablewater supply exhibit midday xylem pressures (  相似文献   

13.
In earlier work tobacco leaves were placed in a Scholander-Hammel pressure bomb and the end of the petiole sealed with a pressure transducer in order to measure pressure transmission from the compressed gas (Pg) in the bomb to the xylem fluid (Px). Pressure bomb theory would predict a 1:1 relationship for Pg:Px when tobacco leaves start at a balance pressure of zero. Failure to observe the expected 1:1 relationship has cast doubt on the pressure-bomb technique in the measurement of the xylem pressure of plants. The experimental and theoretical relationship between Px and Pg was investigated in Tsuga canadensis (L) branches and Nicotiana rustica (L) leaves in this paper. It is concluded that the non 1:1 outcome was due to the compression of air bubbles in embolized xylem vessels, evaporation of water from the tissue, and the expansion of the sealed stem segment (or petiole) protruding beyond the seal of the pressure bomb. The expected 1:1 relationship could be obtained when xylem embolism was eliminated and stem expansion prevented. It is argued that the non 1:1 relationship in the positive pressure range does not invalidate the Scholander pressure bomb method of measuring xylem pressure in plants because Px never reaches positive values during the determination of the balance pressure.  相似文献   

14.
Determination of the pressure in the water-conducting vessels of intactNicotiana rustica L. plants showed that the pressure probe technique gave less-negative values than the Scholander-bomb method. Even though absolute values of the order of −0.1 MPa could be directly recorded in the xylem by means of the pressure probe, pressures between zero and atmospheric were also frequently found. The data obtained by the pressure probe for excised leaves showed that the Scholander bomb apparently did not read the actual tension in the xylem vessles ofNicotiana plants. The possibility that the pressure probe gave false readings was excluded by several experimental controls. In addition, cavitation and leaks either during the insertion of the microcapillary of the pressure probe, or else during the measurements were easily recognized when they occurred because of the sudden increase of the absolute xylem tension to that of water vapour or to atmospheric, respectively. Tension values of the same order could also be measured by means of the pressure probe in the xylem vessels of pieces of stem cut from leaves and roots under water and clamped at both ends. The magnitude of the absolute tension depended on the osmolarity of the bathing solution which was adjusted by addition of appropriate concentrations of polyethylene glycol. Partial and uniform pressurisation of plant tissues or organs, or of entire plants (by means of the Scholander bomb or of a hyperbaric chamber, respectively) and simultaneous recording of the xylem tension using the pressure probe showed that a 1∶1 response in xylem pressure only occurred under a few circumstances. A 1∶1 response required that the xylem vessels were in direct contact with an external water reservoir and/or that the tissue was (pre-)infiltrated with water. Corresponding pressure-probe measurements in isolated vascular bundles ofPlantago major L. orP. lanceolata L. plants attached to a Hepp-type osmometer indicated that the magnitude of the tension in the xylem vessels was determined by the external osmotic pressure of the reservoir. These and other experiments, as well as analysis of the data using classical thermodynamics, indicated that the turgor and the internal osmotic pressure of the accessory cells along the xylem vessels play an important role in the maintenance of a constant xylem tension. This conclusion is consistent with the cohesion theory. In agreement with the literature (P.E. Weatherley, 1976, Philos. Trans. R. Soc. London Ser. B23, 435–444; 1982, Encyclopedia of plant physiology, vol. 12B, 79-109), it was found that the tension in the xylem of intact plants under normal and elevated ambient pressure (as measured with the pressure probe) under quasi-stationary conditions was independent of the transpiration rate over a large range, indicating that the conductance of the flow path must be flow-dependent.  相似文献   

15.
16.
树木树液上升机理研究进展   总被引:8,自引:0,他引:8  
何春霞  李吉跃  郭明 《生态学报》2007,27(1):329-337
水分在植物体内的运输一直是很多植物生理生态学家所关注的一个重要问题。介绍了内聚力学说的基本假设和其存在争议,总结了近年来这一研究领域的几个热点问题,主要包括:(1)木质部栓塞及其恢复机理;(2)木质部压力探针和压力室法测定的木质部张力值不一致的现象及其可能原因;(3)补偿压学说;(4)不同界面层张力以及输水管道的毛细作用力、薄壁细胞膨压和木质部渗透压、逆向蒸腾等在树木汁液上升中的贡献;(5)最近发现的存在于木质部导管伴胞和韧皮部薄壁细胞等质膜中的水孔蛋白在植物水分运输中的调控作用等。这些方面在解释树木的树液上升中都起着重要的作用。  相似文献   

17.
BARCELO  A. ROS 《Annals of botany》1998,82(1):97-103
Production of hydrogen peroxide (H2O2) by the lignifying xylemof several vascular plants has been studied using a new histochemicalmethod based on the H2O2-dependent oxidation of 3,5,3'5'-tetramethylbenzidine(TMB) catalysed by cell wall peroxidases. This method allowsH2O2to be determined in the range of 5–100 µM, whereother methods, such as the KI/starch reagent, fail. With thismethod, it has been possible to determine H2O2production inthe lignifying xylem of a wide range of vascular plants (gymnospermsand angiosperms). The capability of xylem tissues of sustainingH2O2production lends support to the hypothesis that cinnamylalcohol polymerization in xylem vessels is caused by an H2O2-dependentoxidative coupling process.Copyright 1998 Annals of Botany Company H2O2generation, lignification, peroxidase, tetramethylbenzidine, xylem.  相似文献   

18.
Myrothamnus flabellifoliusWelw. is a desiccation-tolerant (‘resurrection’)plant with a woody stem. Xylem vessels are narrow (14 µmmean diameter) and perforation plates are reticulate. This leadsto specific and leaf specific hydraulic conductivities thatare amongst the lowest recorded for angiosperms (ks0.87 kg m-1MPa-1s-1;kl3.28x10-5kg m-1MPa-1s-1, stem diameter 3 mm). Hydraulic conductivitiesdecrease with increasing pressure gradient. Transpiration ratesin well watered plants were moderate to low, generating xylemwater potentials of -1 to -2 MPa. Acoustic emissions indicatedextensive cavitation events that were initiated at xylem waterpotentials of -2 to -3 MPa. The desiccation-tolerant natureof the tissue permits this species to survive this interruptionof the water supply. On rewatering the roots pressures thatwere developed were low (2.4 kPa). However capillary forceswere demonstrated to be adequate to account for the refillingof xylem vessels and re-establishment of hydraulic continuityeven when water was under a tension of -8 kPa. During dehydrationand rehydration cycles stems showed considerable shrinking andswelling. Unusual knob-like structures of unknown chemical compositionwere observed on the outer surface of xylem vessels. These maybe related to the ability of the stem to withstand the mechanicalstresses associated with this shrinkage and swelling.Copyright1998 Annals of Botany Company cavitation, desiccation, hydraulic conductivity, refilling, resurrection plant, root pressure, xylem anatomy,Myrothamnus flabellifolius  相似文献   

19.
Elongation growth of abraded hypocotyl sections of Vigna unguiculataunder xylem perfusion was markedly promoted a few minutes afterthe application of an acid aerosol generated from a solutionof HCl. At the beginning of the acid-induced growth, intracellularpressure (Pi) began to decrease and the membrane potential betweenthe symplast and the xylem apoplast (Vpx) began to depolarize.Subsequently, Pi and Vpx remained at a reduced level and a depolarizedlevel, respectively, while the promotion of elongation growthcontinued for more than 4 hours. The electrogenic componentof the xylem membrane potential (Vpxact) gradually increasedto about twice that before acid treatment. There was a closecorrelation between the enhanced growth and the decrease inintracellular pressure within 30 min after application of acidbut little correltion after 60 min. By contrast, there was littlecorrelation between the promotion of growth and the activityof the xylem pump after 30 min while a close correlation wasobserved after 60 min. It is inferred that the acid-induced activation of water uptakeconsists of two major processes, in series, that are drivenby different forces: the rapid uptake of water for more than30 min, driven by hydrostatic force generated by loosening ofcell walls; and a long-lasting enhancement of water uptake forat least 4 h, which is driven by osmotic force that is generatedby the canal system within the xylem. (Received October 17, 1994; Accepted January 23, 1995)  相似文献   

20.
We report measurements of hydraulic conductivity of Vitis oinifera,Oleo europaea and Populus deltoides 1-year-old twigs. Singleserial internodes were tested for the volume flow rate whichwas related to: (a) the xylem tissue cross-sectional area, (b)the vessel lumina cross-sectional area and (c) the leaf surfacearea supplied by a given stem section. From this, whole xylemhydraulic conductivity (Lx), vessel lumina hydraulic conductivity(Lxv) and leaf specific conductivity (LSC) were calculated.All the three parameters turned out to be linearly related toeach other. This is kcause: (a) the leaf surface area (A1) waslinearly related to xylem cross-sectional area (Ax and (b) theratio of the vessel lumina cross-sectional area (Axv) to xylemcross-sectional area (Ax) was approximately constant along thetwigs. Moreover, the hydraulic conductivity of twig segmentswhere buds grow most actively (distal internodes in V. viniferaand proximal ones in O. europaea) was much lower than in therest of the twigs. A possible role played by these ‘constricted’twig regions is discussed. Key words: Changes, Hydraulic conductivity, Stem  相似文献   

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