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Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters. 总被引:17,自引:12,他引:5
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D Sun P Fajardo-Cavazos M D Sussman F Tovar-Rojo R M Cabrera-Martinez P Setlow 《Journal of bacteriology》1991,173(24):7867-7874
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Use of immunofluorescence to visualize cell-specific gene expression during sporulation in Bacillus subtilis. 总被引:15,自引:6,他引:9
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We have adapted immunofluorescence microscopy for use in Bacillus subtilis and have employed this procedure for visualizing cell-specific gene expression at early to intermediate stages of sporulation. Sporangia were doubly stained with propidium iodide to visualize the forespore and mother cell nucleoids and with fluorescein-conjugated antibodies to visualize the location of beta-galactosidase produced under the control of the sporulation RNA polymerase sigma factors sigma E and sigma F. In confirmation and extension of earlier reports, we found that expression of a lacZ fusion under the control of sigma E was confined to the mother cell compartment of sporangia at the septation (II) and engulfment (III) stages of morphogenesis. Conversely, sigma F-directed gene expression was confined to the forespore compartment of sporangia at postseptation stages of development. Little indication was found for sigma E- or sigma F-directed gene expression prior to septation or in both compartments of postseptation sporangia. Gene expression under the control of the forespore sigma factor sigma G also exhibited a high level of compartmentalization. A high proportion of sporangia exhibited fluorescence in our immunostaining protocol, which should be suitable for the subcellular localization of sporulation proteins for which specific antibodies are available. 相似文献
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J Errington L Appleby R A Daniel H Goodfellow S R Partridge M D Yudkin 《Journal of general microbiology》1992,138(12):2609-2618
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Initiation of endospore formation in Clostridium acetobutylicum 总被引:2,自引:0,他引:2
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