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1.
Tryptophan decarboxylase (TDC), which catalyzes the conversion of Trp to tryptamine, provides a common backbone for many secondary metabolites, and is important in defending plants from abiotic stress such as pathogen infection and insect attack. In this study, we cloned two TDC genes, AeVTDC1 and AeVTDC2, from Ae. variabilis accession No. 1 with resistance to cereal cyst nematode (CCN) and root-knot nematode (RKN). AeVTDC1 and AeVTDC2 encode polypeptides of 510 and 518 amino acids, respectively, and both have a pyridoxal phosphate attachment site and specific catalytic residues. Comparative analyses of gene structure and amino acid motifs revealed that TDCs are highly conserved crossing the analyzed species in monocots and dicots. Phylogenetic analysis indicated that AeVTDCs were closer to TDCs of wheat, Ae. tauschii, Triticum urartu, Brachypodium distachyon, and Hordeum vulgare. Their functions and temporal and spatial expression patterns were investigated. Moreover, AeVTDC1 and AeVTDC2 exhibited different expression responses to the phytohormones abscisic acid, salicylic acid, and methyl jasmonate, suggesting that they may function differently in response to biotic and abiotic stresses. The inhibition of TDC activity with S-αFMT resulted in susceptibility of Ae. variabilis to CCN and RKN, suggesting that TDCs may play important roles in resistance to nematodes. 相似文献
2.
The cereal cyst nematode (CCN) Heterodera avenae, is a significant pathogen of wheat. The wild grass Aegilops variabilis Accession No.1 has been found to be resistant to pathotypes of CCN; at least two genes transferred to wheat, designated as
CreX and CreY, are involved in the resistance response. The CreY gene may be the same as Rkn-mn1, which confers resistance to root knot nematode (RKN) Meloidogyne naasi. The objective of this work was to pyramid the two CCN resistance genes in a wheat background through marker-assisted selection.
As a first step, molecular markers flanking CreX were identified. The completely linked RAPD marker of Rkn-mn1 ( CreY), OpY16- 1065, previously obtained, was converted into a SCAR. All these dominant markers were used to incorporate in the same genotype the
two Ae. variabilis chromosome segments carrying the two genes for resistance. CCN bioassays with the Ha12 pathotype showed that the level of
resistance of the pyramided line was significantly higher than that of CreX and CreY single introgression lines, but lower than that of Ae. variabilis. This study thus illustrates the utilization of molecular markers in breeding for host resistance. 相似文献
3.
In plant tissue, a wound signal is produced at the site of injury and propagates or migrates into adjacent tissue where it induces increased phenylalanine ammonia lyase (PAL, EC 4.3.1.5) activity and phenylpropanoid metabolism. We used excised mid-rib leaf tissue from Romaine lettuce ( Lactuca sativa L., Longifolia) as a model system to examine the involvement of components of the phospholipid-signaling pathway in wound-induced phenolic metabolism. Exposure to 1-butanol vapors or solutions inhibited wound-induced increase in PAL activity and phenolic metabolism. Phospholipases D (EC 3.1.4.4), an enzyme involved in the phospholipid-signaling pathway is specifically inhibited by 1-butanol. Re-wounding tissue, in which an effective 1-butanol concentration had declined below active levels by evaporation, did not elicit the normal wound response. It appears the 1-butanol-treated tissue developed resistance to wound-induced increases in phenylpropanoid metabolism that persisted even when active levels of 1-butanol were no longer present. However, a metabolic product of 1-butanol, rather than 1-butanol itself, may be the active compound eliciting persistence resistance. Inhibiting a subsequent enzyme in the phospholipid-signaling pathway, lipoxygenase (LOX; EC 1.13.11.12) with 1-phenyl-3-pyrazolidinone (1P3P) or reducing the product of LOX activity with diethyldithio-carbamic acid (DIECA) also inhibited wound-induced PAL activity and phenolic accumulation. The effectiveness of 1-butanol, DIECA, and 1P3P declined as the beginning of the 1-h immersion period was delayed from 0 to 4 h after excision. This decline in effectiveness is consistent with involvement of the inhibitors in the production or propagation of a wound signal. The wound signal in lettuce moves into adjacent tissue at 0.5 cm h −1, so delaying application would allow the signal to move into and induce the wound response in adjacent tissue before the delayed application inhibited synthesis of the signal. Salicylic acid (SA) inhibits allene oxide synthase (AOS, EC 4.2.1.92), another enzyme in the phospholipid-signaling pathway. Exposure to 1 or 10 m M SA for 60 min reduced wound-induced phenolic accumulation by 26 or 56%, respectively. However, 1 m M SA lost its effectiveness if applied 3 h after excision, while 10 m M SA remained effective even when applied 4 h after excision. At 1 m M, SA may be perturbing the wound signal through inhibition of AOS, while at 10 m M it appears to have some generally inhibitory effect on subsequent phenolic metabolism. These data further implicate the phospholipid-signaling pathway in the generation of a wound signal that induces phenolic metabolism in wounded leaf tissue. 相似文献
5.
Probenazole (PBZ; 3-allyloxy-1,2-benzisothiazole-1,1-dioxide) is a highly effective chemical inducer of systemic-acquired
resistance (SAR). It has been used widely to protect rice plants against the rice blast fungus Magnaporthe grisea. Previous studies have shown that PBZ induces SAR through enhanced accumulation of salicylic acid (SA). Plants synthesize
SA by either a pathway that uses phenylalanine as substrate or another that involves isochorismate. To clarify how SA is produced
in PBZ-treated Arabidopsis, we examined the expression patterns and enzyme activities of phenylalanine ammonia lyase (PAL) and isochorismate synthase
(ICS), which are the main components of the phenylalanine and isochorismate pathways, respectively. PBZ exposure significantly
improved the accumulation of SA and increased ICS activity. In the sid2–2 mutant, which has a defect in ICS1, PBZ had no effect on the level of endogenous SA or activity of ICS. In contrast, PAL activity and the expression of most
PAL genes were down-regulated by such treatment in wild-type plants. These results suggest that SA is mainly synthesized via
the ICS-mediated pathway in Arabidopsis. 相似文献
6.
Aegilops tauschii (goat grass) is the progenitor of the D genome in hexaploid bread wheat. We have screened more than 200 Ae. tauschii accessions for resistance against leaf rust ( Puccinia triticina) isolates, which are avirulent on the leaf rust resistance gene Lr1. Approximately 3.5% of the Ae. tauschii accessions displayed the same low infection type as the tester line Thatcher Lr1. The accession Tr.t. 213, which showed resistance after artificial infection with Lr1 isolates both in Mexico and in Switzerland, was chosen for further analysis. Genetic analysis showed that the resistance in this accession is controlled by a single dominant gene, which mapped at the same chromosomal position as Lr1 in wheat. It was delimited in a 1.3-cM region between the restriction fragment length polymorphism (RFLP) markers ABC718 and PSR567 on chromosome 5DL of Ae. tauschii. The gene was more tightly linked to PSR567 (0.47 cM) than to ABC718 (0.79 cM). These results indicate that the resistance gene in Ae. tauschii accession Tr.t. 213 is an ortholog of the leaf rust resistance gene Lr1 of bread wheat, suggesting that Lr1 originally evolved in diploid goat grass and was introgressed into the wheat D genome during or after domestication of hexaploid wheat. Compared to hexaploid wheat, higher marker polymorphism and recombination frequencies were observed in the region of the Lr1 ortholog in Ae. tauschii. The identification of Lr1Ae, the orthologous gene of wheat Lr1, in Ae. tauschii will allow map-based cloning of Lr1 from this genetically simpler, diploid genome.Hong-Qing Ling and Jiwen Qiu have contributed equally to this work 相似文献
8.
The response of tobacco ( Nicotiana tabacum L. cv. Xanthinc) plants, epigenetically suppressed for phenylalanine ammonia-lyase (PAL) activity, was studied following infection by tobacco mosaic virus (TMV). These plants contain a bean PAL2 transgene in the sense orientation, and have reduced endogenous tobacco PAL mRNA and suppressed production of phenylpropanoid products. Lesions induced by TMV infection of PAL-suppressed plants are markedly different in appearance from those induced on control plants that have lost the bean transgene through segregation, with a reduced deposition of phenofics. However, they develop at the same rate as on control tobacco, and pathogenesis-related (PR) proteins are induced normally upon primary infection. The levels of free salicylic acid (SA) produced in primary inoculated leaves of PAL-suppressed plants are approximately fourfold lower than in control plants after 84 h, and a similar reduction is observed in systemic leaves. PR proteins are not induced in systemic leaves of PAL-suppressed plants, and secondary infection with TMV does not result in the restriction of lesion size and number seen in control plants undergoing systemic acquired resistance (SAR). In grafting experiments between wild-type and PAL-suppressed tobacco, the SAR response can be transmitted from a PAL-suppressed root-stock, but SAR is not observed if the scion is PAL-suppressed. This indicates that, even if SA is the systemic signal for establishment of SAR, the amount of pre-existing phenylpropanoid compounds in systemic leaves, or the ability to synthesize further phenylpropanoids in response to the systemic signal, may be important for the establishment of SAR. Treatment of PAL-suppressed plants with dichloro-isonicotinic acid (INA) induces PR protein expression and SAR against subsequent TMV infection. However, treatment with SA, while inducing PR proteins, only partially restores SAR, further suggesting that de novo synthesis of SA, and/or the presence or synthesis of other phenylpropanoids, is required for expression of resistance in systemic leaves. 相似文献
9.
The high-molecular-weight (HMW) glute-nin subunit composition of seven species from the Cylindropyrum and Vertebrata sections of the Aegilops genus was studied using SDS-PAGE and Western blot analysis. Two subunits were detected in Ae. caudata and three in Ae. cylindrica. In both species, subunits showing electrophoretic mobility similar to that of 1Dx2 were present. Western blot analysis using
a monoclonal antibody (IFRN 1602) specific for the 1Ax and 1Dx subunits of bread wheat showed that the 1Dx-like subunit of
Ae. caudata gave only a weak reaction. This indicates that Ae. caudata expresses subunits which are more distantly related to the 1Dx subunits. Two subunits were detected in each of the 60 accessions
of Ae. tauschii, including several 1D tx subunits showing different electrophoretic mobilities from those of the 1Dx subunits commonly found in bread wheat. All
of the 1D tx subunits reacted strongly with IFRN 1602, confirming their close relationship to the 1Dx subunits of bread wheat. Three
subunits were found in Ae. crassa (6 x), four in Ae. ventricosa and Ae. juvenalis and five in Ae. vavilovii. In these four species, the subunits that showed electrophoretic mobility similar, or close, to that of 1Dx2 all reacted
with IFRN 1602. In addition, Ae. ventricosa contained a subunit showing electrophoretic mobility slower than that of 1Dx2.2, which also reacted with IFRN 1602. These
results suggest that the D-genome component in the multiploid Aegilops species express at least one HMW glutenin subunit that is structurally related to the 1Dx subunits of bread wheat.
Received: 5 November 1999 / Accepted: 12 February 2000 相似文献
10.
Cell suspension cultures (cell line No 615) of Catharanthus roseus cv. Little Delicata responded to elicitor treatment by accumulating monoterpenoid indole alkaloids and phenolic compounds. The excretion of phenols into the culture medium resulted from the induction of the branch-point enzyme phenylalanine ammonia lyase. The accumulation of alkaloids, however, occurred several hours earlier than the elicitor-mediated induction of tryptophan decarboxylase through which shikimate pathway intermediates are channelled into tryptamine and related indole alkaloids. The results indicate that both pathways for phenol and indole alkaloid biosynthesis responded to elicitor treatment and that no obvious causal relationship between pathways could be deduced from this study.Abbreviations PAL
phenylalanine ammonia lyase
- TDC
tryptophan decarboxylase
Dedicated to Dr. Friedrich Constabel on the occasion of his 60th birthday 相似文献
11.
Phenylpropanoid derivatives are a complex class of secondary metabolites that have many important roles in plants during normal growth and in responses to environmental stress. Phenylalanine ammonialyase (PAL) catalyzes the first step in the biosynthesis of phenylpropanoids, and is usually encoded by a multi-gene family. Genomic clones for three Arabidopsis thaliana PAL genes containing the entire protein-coding region and upstream and downstream sequences have been obtained and completely sequenced. Two A. thaliana PAL genes ( PAL1 and PAL2) are structurally similar to PAL genes that have been cloned from other plant species, with a single intron at a conserved position, and a long highly conserved second exon. Previously identified promoter motifs plus several additional sequence motifs were found in the promoter regions of PAL1 and PAL2. Expression of PAL1 and PAL2 is both qualitatively and quantitatively similar in different plant organs and under various inductive conditions. A third A. thaliana PAL gene, PAL3, differs significantly from PAL1 and PAL2 and other sequenced plant PAL genes. PAL3 contains an additional intron, and its deduced amino acid sequence is less homologous to other PAL proteins. The PAL3 promoter region lacks several sequence motifs conserved between A. thaliana PAL1 and PAL2, as well as motifs described in other genes involved in phenylpropanoid metabolism. A. thaliana PAL3 was expressed at very low levels under the conditions examined. 相似文献
12.
Herbivory damage leads to induction of rapid signals and responses in plants such as oxidative burst, accumulation of secondary metabolites and defensive proteins. Response of various defensive enzymes and secondary metabolites in flag leaf samples of six bread wheat varieties against aphid feeding was investigated. Six bread wheat varieties, namely PBW 621 and HD 2967 (timely sown irrigated), PBW 590 and PBW 658 (late sown irrigated), and PBW 644 and PBW 660 (timely sown rainfed) were grown under the aphid infested and uninfested conditions and were sampled at a regular interval to analyze the biochemical changes caused by aphid feeding. A tremendous increase in the overall activity of various enzymes namely superoxide dismutase, glutathione reductase, phenylalanine ammonia lyase and polyphenol oxidase was observed, all of which play an important role in plants defense towards aphid feeding. Each wheat genotype showed an overall difference in their defensive activity towards aphid feeding. However, certain genotypes under different conditions showed significantly less susceptibility towards aphid damage. Abbreviations: GR: glutathione reductase; HPR: host plant resistance; PAL: phenylalanine ammonia lyase; PPO: poly phenol oxidase; POD: peroxidase; SOD: superoxide dismutase 相似文献
15.
Blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Phenylalanine ammonia lyase (PAL) is a key enzyme in the phenylpropanoid pathway, which leads to the biosynthesis of defense‐related phytohormone salicylic acid (SA) and flavonoid‐type phytoalexins sakuranetin and naringenin. However, the roles and biochemical features of individual rice PALs in defense responses to pathogens remain unclear. Here, we report that rice OsPAL06, which can catalyze the formation of trans‐cinnamate using l ‐phenylalanine, is involved in rice root– M. oryzae interaction. Os PAL06‐knockout mutant showed increased susceptibility to M. oryzae invaded from roots and developed typical leaf blast symptoms, accompanied by nearly complete disappearance of sakuranetin and naringenin and a two‐third reduction of the SA level in roots. This mutant also showed compensatively induced expression of chalcone synthase, which is involved in flavonoid biosynthesis, isochorismate synthase 1, which is putatively involved in SA synthesis via another pathway, reduced jasmonate content and increased ethylene content. These results suggest that OsPAL06 is a positive regulator in preventing M. oryzae infection from roots. It may regulate defense by promoting both phytoalexin accumulation and SA signaling that synergistically and antagonistically interacts with jasmonate‐ and ethylene‐dependent signaling, respectively. 相似文献
16.
苯丙氨酸解氨酶(phenylalanine ammonia lyase, PAL)是苯丙烷类代谢途径的关键酶,催化苯丙氨酸转化为肉桂酸,促进黄酮、香豆素等次生代谢物的生成。本文根据已克隆的麻疯树苯丙氨酸解氨酶基因JcPAL的序列设计引物,通过DNA步移技术,克隆出长度为1 334 bp的JcPAL基因起始密码子上游序列。序列分析显示其不仅具备CAAT、TATA盒这些保守元件,而且包含多种胁迫诱导元件,特别是在序列中发现一些苯丙氨酸解氨酶特有的元件。为了鉴定JcPAL基因的启动子元件,分别将长度不同的5′端侧翼区缺失体定向插入载体pBI121中, 取代原有的CaMV35S启动子,构建了4个驱动报告基因GUS的植物表达载体。 相似文献
17.
Hexaploid bread wheat was derived from a hybrid cross between a cultivated form of tetraploid Triticum wheat (female progenitor) and a wild diploid species, Aegilops tauschii Coss. (male progenitor). This cross produced a fertile triploid F 1 hybrid that set hexaploid seeds. The identity of the female progenitor is unknown, but various cultivated tetraploid Triticum wheats exist today. Genetic and archaeological evidence suggests that durum wheat ( T. turgidum ssp. durum) may be the female progenitor. In previous studies, however, F 1 hybrids of durum wheat crossed with Ae. tauschii consistently had low levels of fertility. To establish an empirical basis for the theory of durum wheat being the female progenitor of bread wheat, we crossed a durum wheat cultivar that carries a gene for meiotic restitution with a line of Ae. tauschii. F 1 hybrids were produced without using embryo rescue techniques. These triploid F 1 hybrids were highly fertile and spontaneously set hexaploid F 2 seeds at the average selfed seedset rate of 51.5%. To the best of our knowledge, this is the first example of the production of highly fertile F 1 hybrids between durum wheat and Ae. tauschii. The F 1 and F 2 hybrids are both similar morphologically to bread wheat and have vigorous growth habits. Cytological analyses of F 1 male gametogenesis showed that meiotic restitution is responsible for the high fertility of the triploid F 1 hybrids. The implications of these findings for the origin of bread wheat are discussed. 相似文献
18.
Pre-inoculation of plants with a pathogen that induces necrosis leads to the development of systemic acquired resistance (SAR) to subsequent pathogen attack [1]. The phenylpropanoid-derived compound salicylic acid (SA) is necessary for the full expression of both local resistance and SAR [2] [3]. A separate signaling pathway involving jasmonic acid (JA) is involved in systemic responses to wounding and insect herbivory [4] [5]. There is evidence both supporting and opposing the idea of cross-protection against microbial pathogens and insect herbivores [6] [7]. This is a controversial area because pharmacological experiments point to negative cross-talk between responses to systemic pathogens and responses to wounding [8] [9] [10], although this has not been demonstrated functionally in vivo. Here, we report that reducing phenylpropanoid biosynthesis by silencing the expression of phenylalanine ammonialyase (PAL) reduces SAR to tobacco mosaic virus (TMV), whereas overexpression of PAL enhances SAR. Tobacco plants with reduced SAR exhibited more effective grazing-induced systemic resistance to larvae of Heliothis virescens, but larval resistance was reduced in plants with elevated phenylpropanoid levels. Furthermore, genetic modification of components involved in phenylpropanoid synthesis revealed an inverse relationship between SA and JA levels. These results demonstrate phenylpropanoid-mediated cross-talk in vivo between microbially induced and herbivore-induced pathways of systemic resistance. 相似文献
20.
We examined basal defense responses and cytomolecular aspects of riboflavin-induced resistance (IR) in sugar beet-Rhizoctonia solani pathsystem by investigating H(2)O(2) burst, phenolics accumulation and analyzing the expression of phenylalanine ammonia-lyase (PAL) and peroxidase (cprx1) genes. Riboflavin was capable of priming plant defense responses via timely induction of H(2)O(2) production and phenolics accumulation. A correlation was found between induction of resistance by riboflavin and upregulation of PAL and cprx1 which are involved in phenylpropanoid signaling and phenolics metabolism. Application of peroxidase and PAL inhibitors suppressed not only basal resistance, but also riboflavin-IR of sugar beet to the pathogen. Treatment of the leaves with each inhibitor alone or together with riboflavin reduced phenolics accumulation which was correlated with higher level of disease progress. Together, these results demonstrate the indispensability of rapid H(2)O(2) accumulation, phenylpropanoid pathway and phenolics metabolism in basal defense and riboflavin-IR of sugar beet against R. solani. 相似文献
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