A new high‐speed cellular imaging method using clinically applicable moxifloxacin labeling, called as moxifloxacin based confocal microscopy (MBCM), was developed for fast and sensitive tumor detection and delineation. The MBCM demarcated malignant brain tumor from normal brain by visualizing dense and irregular cell distribution in the tumor. An image processing algorithm was developed for automated brain tumor detection. Further details can be found in the article by Seunghun Lee, Won Yeong Park, Hoonchul Chang, et al. ( e201900197 ).
A type of compact and cost‐effective light‐sheet imaging device, termed sub‐voxel‐resolving light‐sheet add‐on module (SLAM), is developed to cooperate with conventional 2D epifluorescence microscope, allowing high‐contrast, resolution‐improved 3D imaging of various biological samples at high throughput. Further details can be found in the article by Fang Zhao, Yicong Yang, Yi Li, et al. ( e201960243 ).
This study proposed a Sparse‐Graph Manifold Learning (SGML) method to balance the sparseness and morphology preserving for bioluminescence tomography reconstruction. It inherits the benefits of non‐convex sparsity constraint and dynamic Laplacian graph model. The results of numerical simulations and in vivo experiments demonstrate that the proposed method yields accurate and robust results in terms of tumor spatial location and morphology recovery. Further details can be found in the article by Hongbo Guo, Ling Gao, Jingjing Yu, et al. ( e201960218 )
A false‐colored and merged image of fresh, ex vivo rat kidney acquired using an excitation‐scanning hyperspectral imaging system. The spectral image was acquired using excitation wavelengths from 360 to 550 nm. Colors represent principal components extracted from a spectral image cube featuring no added labels or markers. Further details can be found in the article by Peter F. Favreau, Joshua A. Deal, Bradley Harris, et al. ( e201900183 ).
Top: Illustration of how to obtain 3D embryo images from a locust egg using OCT B‐scans. The internal structures of the embryo can be clearly identified as it develops. Bottom: The real time observation of katatrepsis and twist of a lowland locust embryo on days 7‐8, as the embryo develops. The embryonic movements can be readily identified by tracking the positions of the embryo's eyes (E1 and E2). Further details can be found in the article by Ya Su, Liya Wei, Hao Tan, et al. ( e201960047 ).
The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
Optical Coherence Tomography angiography (OCTA) is widely used to image chorioretinal vasculature, with contrast that derives from scattering and motion of red blood cells (RBCs). In the rat eye, a scattering tracer highlights vertical vessels which are not visualized by intrinsic RBC scattering alone. The dependence of microvessel OCTA on angular orientation, explored in this work, represents a potential artifact that should be considered in the clinical interpretation of OCTA. Further details can be found in the article by Jun Zhu, Marcel T. Bernucci, Conrad W. Merkle, and Vivek J. Srinivasan ( e202000090) .
A multi‐wavelength Spatial Frequency Domain Imaging (SFDI) utilizes structured illumination to provide absorption and reduced scattering coefficient maps of colorectal tissue. Combining SFDI with a Machine Learning algorithm ‐ AdaBoost, different types of colorectal tissues including normal, adenomatous polyp and cancer, can be differentiated with high accuracy. This new technique provides a potential method to assist in colorectal cancer screening. Further details can be found in the article by Shuying Li, Yifeng Zeng, William C. Chapman Jr, et al. ( e201960241 ).
Successful therapy of twin‐to‐twin transfusion syndrome requires accurate imaging to guide laser photocoagulation of the anastomosing placental vessels. Photoacoustic (PA) imaging is an alternative imaging method that provides contrast for hemoglobin, and in this study, it was used to visualize chorionic superficial and subsurface vasculature in human placentas. The strong potential of PA imaging to guide minimally invasive fetal therapies was demonstrated. Further details can be found in the article by Efthymios Maneas, Rosalind Aughwane, Nam Huynh, et al. ( e201900167 ).
A 3D printed stereotaxic head mount that enables high resolution imaging of the anterior chamber of the eye in alert and freely mobile mice is designed in this study. The system is placed non‐invasively using temporal mount bars and a snout mount, without breaking the skin or risking suffocation, while an endoscopic channel stabilizes the ocular probes. Further details can be found in the article by Bjorn Paulson, Sangwook Lee, Miyeon Jue, Kyungsung Lee, Sanghwa Lee, Guk Bae Kim, Youngjin Moon, Joo Yong Lee, Namkug Kim, and Jun Ki Kim ( e201960188 ).
This study presents a novel intraoperative in vivo imaging approach which harnessed Cerenkov luminescence (CL) to detect primary and metastatic colorectal cancer (CRC) using clinically approved radiopharmaceuticals. In the mice and swine experiments, the proposed approach effectively improved the effect of CRC surgery. The approach is believed to be promising for utilizing CL in open surgery. Further details can be found in the article by Zeyu Zhang, Yawei Qu, Yu Cao et al. ( e201960152 )
Infrared attenuated total reflection spectroscopy is an emerging label‐free method for analyzing the degree of damage in cartilage samples. In the present study, sheep menisci have been characterized after meniscectomy via the variances of relevant biomolecules at the incision surface. Further details can be found in the article by Angela I. López‐Lorente et al. ( e201800429 ).
The figure shows the detailed morphology of vasculature and dynamic changes of the blood vessel diameter and density and the oxygen saturation in the blood vessels in fetal brain after acute prenatal ethanol exposure in the second‐trimester equivalent murine model obtained using a real‐time photoacoustic tomography (PAT) system. Further details can be found in the article by Tianqi Shan, Yuan Zhao, Shixie Jiang, Huabei Jiang ( e201960161 ).
Intraoperative neurosurgical diagnosis of brain tumor determines the success rate of patients' prognoses. We firstly proposed a novel approach based on an optical method to distinguish the site of the tumor functionally. The promoted technique is non‐invasive, non‐radioactive, dye‐free, and potential of real‐time monitoring, which is still not available nowadays by using other techniques. The method could be applied to neuroimage guiding system for precision surgery of brain. Further details can be found in the article by Xin‐Rui Liu, Tien‐Yu Hsiao, Yun‐Qian, et al. ( e201900200 ).
Confocal Raman microscopy provides composition and constitution of label-free samples at high spatial-resolution. In this study, the histology of atherosclerotic arteries was measured using a custom-built confocal Raman microscopy that was developed to improve imaging speed, diffraction efficiency, and spectral resolution. Additionally, the machine learning method was used to enhance the accuracy of classification. Results show that each layer of arteries and lipid-rich inflamed plaque can be successfully characterized. Further details can be found in the article by Jingchao Xing, Dong-Ryoung Lee, Jin Won Kim, and Hongki Yoo ( e202200243 ).
iSERS (SERS=surface‐enhanced Raman scattering) microscopy is an emerging Raman‐based staining technique for the selective localization of target proteins on cells and tissues using antibody‐ SERS nanotag conjugates. In this contribution we demonstrate the feasibility of iSERS for imaging of programmed cell death‐ligand 1 (PD‐L1), an important predictive biomarker, on single SkBr‐3 breast cancer cells. Further details can be found in the article by Elzbieta Stepula, Matthias König, Xin‐Ping Wang, et al. ( e201960034 ).
This work addresses an unmet clinical need, that of glaucoma monitoring through intraocular pressure (IOP) interrogation in patients with artificial corneas (keratoprost hesis). We demonstrate direct integration of a low‐drift, fiber‐optic Fabry‐Perot pressure sensor embedded in the keratoprosthesis via rapid, non‐contact micromagnetic fiber alignment. IOP interrogation is achieved by using white‐light interferometry, which provides sub‐mmHg IOP sensitivity. Further details can be found in the article by Pui‐Chuen Hui, Katia Shtyrkova, Chengxin Zhou, et al. ( e202000031 ).
Photodynamic inactivation of prions by disulfonated hydroxyaluminum phthalocyanine. Further details can be found in the article by Marie Kostelanska, Jaroslav Freisleben, Zdenka Backovska Hanusova, et al. ( e201800430 ).
