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Iu A Knirel' N A Kocharova A S Shashkov B A Dmitriev N K Kochetkov 《Bioorganicheskaia khimiia》1986,12(10):1384-1390
Polysaccharide chains of P. aeruginosa O5a, b, c, O5a, b, d and O5a, d (Lányi classification) lipopolysaccharides contain D-xylose, N-acetyl-D-fucosamine (FucNAc) and a derivative of 5,7-diamino-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid, PseN2) carrying acetyl or (R)-3-hydroxybutyryl (Hb) and formyl (Fm) groups as N-acyl substituents. Degradation of the lipopolysaccharides with dilute acetic acid caused depolymerisation of the polysaccharide chains as a result of cleavage of glycosidic linkage of pseudaminic acid to give trisaccharides representing chemical repeating units of the polysaccharides. Basing on analysis of the trisaccharides using 1H and 13C NMR spectroscopy and mass-spectrometry, the following structures of the polysaccharide chains were established: (Formula: see text). O5a, d polysaccharide is identical to P. aeruginosa immunotype 6 O-specific polysaccharide. 相似文献
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Y A Knirel I M Skvortsov A S Shashkov B A Dmitriev N K Kochetkov E S Stanislavsky G M Mashilova 《European journal of biochemistry》1985,150(3):551-557
Lipopolysaccharides were isolated from the phenol layer on aqueous phenol extraction of cells of Pseudomonas aeruginosa O11 (Lányi classification), strains 170021 and 170040. On mild acid degradation of the lipopolysaccharides, with the subsequent gel-filtration on Sephadex G-50, neutral O-specific polysaccharides made up of 6-deoxysugars alone were obtained. Two 2-acetamido-2,6-dideoxy-L-galactose (LFucNAc), 2-acetamido-2,6-dideoxy-D-glucose (DQuiNAc) and L-rhamnose (LRha) residues were found to be the components of the strain 170021 polysaccharide repeating units; those of strain 170040 contained the same monosaccharides, but, instead of 2-acetamido-2,6-dideoxy-D-glucose residue, that of 2-acetamido-2,6-dideoxy-D-galactose (DFucNAc) was present. On the basis of the 13C nuclear magnetic resonance data, methylation analysis and three successive Smith degradations the following structures were determined for the polysaccharide repeating units: strain 170021----2) LRha(alpha 1----3)LFucNAc(alpha 1----3)LFucNAc(alpha 1----3)DQuiNAc(beta 1----; strain 170040,----2)LRha(alpha 1----3)LFucNAc-(alpha 1----3)LFucNAc(alpha 1----3)DFucNAc(beta 1----; differing from one another by configuration of C-4 of 2-acetamido-2,6-dideoxy-D-hexopyranose only. 相似文献
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There is a special emphasis today on integrating traditional healing within health services. However, most areas in which
there is a system of traditional healing have undergone colonization and a number of pressures suppressing tradition for hundreds
of years. The question arises as to how one can understand today’s tradition in light of earlier traditions. This article
is based on material collected in Sámi areas of Finnmark and Nord-Troms Norway; it compares local healing traditions with
what is known of earlier shamanic traditions in the area. The study is based on 27 interviews among healers and their patients.
The findings suggest that although local healing traditions among the Sámi in northern Norway have undergone major transformations
during the last several hundred years, they may be considered an extension of a long-standing tradition with deep roots in
the region. Of special interest are also the new forms tradition may take in today’s changing global society. 相似文献
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