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1.
《The International journal of biochemistry》1989,21(5):525-529
- 1.1. Effects of antioxidants (butylated hydroxytoluene and nor-dihydroguaiaretic acid), vitamin K-related quinones (vitamin K1 and coenzyme Q10) and inorganic copper (CuSO4), in concentrations inhibiting NADPH: cytochrome P -450 reductase, were re-examined on benzo(a)pyrene metabolism in mouse liver uninduced microsomes.
- 2.2. It was found that all these compounds decrease production of the two-electron oxygenation products of benzo(a)pyrene (monophenoles, diols) and the amounts of glucuronides in a manner parallel to their inhibitory potency against NADPH: cytochrome P-450 reductase.
- 3.3. No correlation was found between amounts of one-electron oxidation products of benzo(a)pyrene and inhibition of NADPH: cytochrome P-450 reductase.
- 4.4. Without added UDPGA the compounds studied decreased protein associated benzo(a)pyrene metabolites in parallel to the decreased overall metabolism of this polyaromatic hydrocarbon.
- 5.5. The mode of action of the studied compounds is discussed.
2.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1980,65(1):115-119
- 1.1. Albumin purified from rhesus monkey (MSA) shows immunological cross-reactivity with human serum albumin (HSA) by RIA.
- 2.2. The amino-terminal sequence of MSA shows a high degree of homology to HSA.
- 3.3. Thirty minutes after injection of radioactive leucine directly into the portal vein, albumin was purified chemically from the liver, kidneys and serum.
- 4.4. At this time, 15% of the label was incorporated into liver homogenate protein.
- 5.5. A highly labelled immunoreactive albumin form was purified from liver to constant specific radioactivity and separated from tissue and serum albumin.
- 6.6. The specific radioactivity of this proalbumin was 36-times higher than the specific radioactivity of albumin in liver tissue.
- 7.7. These similarities to HSA suggest that this non human primate species can serve as a useful model of human albumin synthesis in vivo.
3.
《The International journal of biochemistry》1977,8(4):323-327
- 1.1. The results on the distribution of lysosomal hydrolases indicated that the specific activity of acid phosphatase is 5 times higher in glomeruli compared to tubular fraction. The activity of gb-galactosidase was 4 times higher m tubules compared to glomeruli.
- 2.2. Sephadex G-150 gel chromatography of soluble fraction of cortex homogenate, glomeruli and tubules indicated that the enzyme acid phosphatase occurs in multiple forms designated as peaks I, II and III.
- 3.3. The specific activity of peak II was 12–15 times higher in glomeruli compared to the cortex homogenate, and very low in tubules.
- 4.4. Arylsulphatases A and B were also 2–3 times higher in the glomerular fraction compared to the tubules.
4.
《The International journal of biochemistry》1976,7(12):633-634
- 1.1. Plasma membranes have been isolated from frog (Rana esculenta) liver.
- 2.2. The average yield was 0.194 mg protein/g wet liver.
- 3.3. The activities of plasma membrane-bound enzymes (Na+-K+-ATPase and 5'-nucleotidase as well as of (Mg2+)-ATPase have been determined in the liver homogenate and in isolated plasma membranes.
- 4.4. (Na+-K+-ATPase, 5'-nucleotidase and (Mg2+)-ATPase activities of frog liver are compared with the same enzymatic activities observed in rat liver.
5.
《The International journal of biochemistry》1984,16(7):713-720
- 1.1. The microsomal flavin-containing monooxygenase has been purified from mouse and pig liver utilizing Cibacron-Blue Sepharose, Procion-Red agarose, and 2'5'-ADP Sepharose.
- 2.2. The enzymes had a final specific activity of 1200 and 954 nmol/min/mg protein from mouse and pig liver respectively.
- 3.3. The enzyme from both mouse and pig liver displayed typical flavoprotein spectra and appeared homogeneous by denaturing polyacrylamide gel electrophoresis.
6.
《The International journal of biochemistry》1993,25(6):879-884
- 1.1. Phospholipase A2 was isolated from Agkistrodon bilineatus venom by Sephadex G-75 and CM-Cellulose column chromatographies.
- 2.2. The purified phospholipase A2-I gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis.
- 3.3. The enzyme preparation had a molecular weight of 14,000, isoelectric point of pH 8.77 and possessed 123 amino acid residues.
- 4.4. The purified phospholipase A2 possessed lethal, indirect hemolytic and anticoagulant activities.
- 5.5. The enzyme hydrolyzed the phospholipids phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI) and phosphatidyl serine (PS).
- 6.6. The concentration of mouse diaphragm was inhibited and the contraction of guinea pig left atrium was increased by phospholipase A2-I.
- 7.7. Phospholipase A2 activity of this preparation was inhibited by ethylenediamine tetraacetic acid, p-bromo phenacyl bromide, n-bromo succinimide or dithiothreitol, but not by diisopropyl fluorophosphate or benzamidine.
7.
《The International journal of biochemistry》1980,11(4):605-618
- 1.1. L-Glutamine conversion into ammonia, urea and glucose by the perfused liver of 48 hr starved guinea-pigs was concentration dependent attaining the maximal rate at 4 mM.
- 2.2. The activity of glutaminase I (EC 3.5.12), measured in isolated liver mitochondria was high enough to account for the observed rate of ammonia, urea and glucose formation by the perfused liver. Neither NH4C1 (5 mM) nor aminooxyacetate (0.5 mM) affected the rate of glutamine conversion into glutamate by isolated liver mitochondria.
- 3.3. Gluconeogenesis and ureogenesis from glutamine was inhibited by octanoate, Dt-3-hydroxybutyrate, aminooxyacetate, ethanol and p-hydroxyphenylpyruvate while ammonia formation was stimulated by aminooxyacetate. 2,4-Dinitrophenol stimulated the rate of the formation of all three metabolites from glutamine.
- 4.4. The major changes induced by aminooxyacetate, as determined in livers perfused with glutamine and stopped by freeze-clamping technique, consisted in a decrease in the content of ATP, aspartate and malate and in a slight increase in the content of glutamate.
- 5.5. Glutamine is an effective precursor of phosphoenolpyruvate in isolated liver mitochondria. Its formation was inhibited by octanoate and by DL-3-hydroxybutyrate.
- 6.6. The data are discussed in terms of regulation of glutamine catabolism in liver with emphasis on ureogenesis and gluconeogenesis.
8.
《The International journal of biochemistry》1981,13(4):471-482
- 1.1. Glucose formation from lactate by the perfused liver of 48 hr starved chickens was strongly inhibited by adenosine (Ado); the half-maximal inhibition was attained at 40 μM. This effect was paralleled by a four- to five-fold increase of ATP content as determined in freeze-clamped liver.
- 2.2. In chicken liver homogenate gluconeogenesis from precursors such as alanine, glutamate, glutamine and aspartate, which are not converted into glucose by the perfused chicken liver, proceeded at rates equal to or higher than that with lactate, being markedly inhibited by Ado.
- 3.3. In the perfused guinea-pig liver glucose synthesis with lactate, propionate, glycerol and fructose was also inhibited by Ado; however, when precursors such as pyruvate, glutamine and a mixture of lactate + pyruvate were supplied to the liver Ado did not inhibit gluconeogenesis.
- 4.4. Assay of adenine nucleotides in the perfused guinea-pig liver, stopped by freeze-clamping technique in a number of experimental variants, revealed no correlation between the rate of gluconeogenesis and the changes induced by Ado in the adenine nucleotide pool.
- 5.5. In the perfused liver of both chicken and guinea-pig Ado produced an increase of the lactate to pyruvate ratio and, in general, a diminution of the content of malate-aspartate shuttle intermediates.
- 6.6. The results are interpreted as suggesting that the inhibitory effect of Ado on hepatic gluconeogenesis is not necessarily mediated by the changes in the adenine nucleotide pool.
9.
- 1.1. The overall effect of handling, anaesthesia and sham injection on some blood metabolites, liver glycogen and several key enzymes involved in liver carbohydrates and nitrogen metabolism was studied in rainbow trout. In addition, the possible role of anaesthesia (MS222) itself as a stress-inductor or suppressor was also studied.
- 2.2. Stress resulted in hyperglycaemia and initially in liver glycogen depletion, as well as increasing plasma amino acid levels.
- 3.3. Glycogen stores subsequently recovered while amino acid concentration fell.
- 4.4. These changes seemed to correlate with the increased activity of liver fructose 1,6-bisphosphatase, glucose 6-phosphate dehydrogenase, alanine aminotransferase and glutamate dehydrogenase, thus supporting the hypothesis that gluconeogenic flux from amino acids increases in stressed trouts.
- 5.5. Anaesthesia, under the same experimental conditions, did not seem to mediate in stress production, but rather resulted in stress suppression.
10.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1981,68(3):515-520
- 1.1. Fatty acid synthetase from liver of cold and warm adapted flounder and rabbit was purified to homogenity and compared.
- 2.2. The mol. wt of the cold and warm flounder enzyme was estimated to be about 457,000.
- 3.3. The kinetic properties were found to be similar for warm and cold adapted flounder liver enzyme and not different from the rabbit liver enzyme when measured at 5, 10, 15, 20 and 37°C.
- 4.4. Palmitic acid was the main product of both the flounder and rabbit enzyme, but significant amounts of butyric acid were also synthesized. The product composition did not change for any of the enzymes tested when the incubation temperature was changed.
- 5.5. It was concluded that fatty acid synthetase from flounder liver is similar to mammalian fatty acid synthetase with regard to molecular weight and kinetic properties.
11.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1987,86(2):551-555
- 1.1. Growing male kittens were fed an 18% casein diet supplemented with 2, 3, or 4% l-methionine (MET) for 6 weeks.
- 2.2. Free MET concentration in liver increased 30-fold and cystathionine two- to three-fold; the activity of adenosyl-MET transferase and cystathionase also increased but remained lower than previously found in rats.
- 3.3. Taurine concentration in liver decreased in cats fed excess MET and appeared to depend on taurine intake.
- 4.4. Alanine aminotransferase activity was high in all groups while serine dehydratase activity was very low.
- 5.5. Pyruvate kinase and malic enzyme activities which are normally low in cat liver increased after excess MET. Also, glucose 6-phosphate and 6-phosphogluconate dehydrogenases increased.
- 6.6. Cat liver metabolism showed limited adaptation to an excess dietary intake of methionine compared to that found in rats.
12.
《The International journal of biochemistry》1980,11(1-2):209-214
- 1.1. Puromycin aminonucleoside (PA) nephrosis may constitute a good experimental model to investigate the involvement of the cGMP system in the regulation of several kidney functions and especially glomerular permeability.
- 2.2. After a single intravenous injection of PA we studied the evolution of the guanylate cyclase (GCase) and cGMP-phosphodiesterase (G-PDE) activities in pure preparations of glomeruli (Gl) and tubules (TU).
- 3.3. Both Gl and TU homogenates showed a strong increase of the GCase activity 12 days after PA injection.
- 4.4. In the presence of Triton X-100, TU homogenate GCase showed the same pattern as in absence of this detergent while the Gl enzyme decreased unexpectedly. On the other hand, the only G-PDE change was observed in the TU where this activity decreases progressively.
- 5.5. Gl pellets and TU supernatants showed similar changes as in total homogenates. But, compared to the total homogenate, both Gl and TU supernatant GCases were strongly activated and in the Gl these activation rates were not the same in normal and 12 days-nephrotic rats.
- 6.6. These results could be explained by the existence of a membrane bound GCase “effector” involved in the physiopathological evolution of the disease.
- 7.7. In conclusion it seems to be clear that the cGMP-system is involved in the evolution of PA-nephrosis. But the precise relation between variations in the cGMP-system and the disease remains unclear and needs further investigation.
13.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1980,65(1):147-150
- 1.1. An esterase which hydrolyzes 4-nitrophenyl(phenyl)phosphonic acid (4-NPPP) was purified from M. senile (sea anemone).
- 2.2. The enzyme showed no 5′-nucleotide phosphodiesterase activity with 5′-(4-nitrophenyl) TMP or phosphomonoesterase activity with 4-nitrophenylphosphate.
- 3.3. Addition of excess Zn2+ restored activity after inactivation by EDTA.
- 4.4. Thiol reagents and phenylmenthanesulfonylfluoride did not inactivate, whereas, dithiothreitol inactivated.
- 5.5. Aminoethylphosphonic acid (AEP) was a competitive inhibitor of 4-NPPP indicating possible activity with phosphonomonoesters of AEP.
14.
《Comparative biochemistry and physiology. A, Comparative physiology》1990,95(1):139-144
- 1.1. Common carp (Cyprinus carpio) exposed to experimental temperatures of 12, 18, 24, 30 or 36°C for a 4-week period were used to investigate the effect of temperature acclimation on the frequency of opercular movement (FOM), growth and cytochrome c oxidase (CCO) activity in heart, liver and muscle.
- 2.2. An exponential relationship between FOM and temperature after the first week (1010 =1.76) disappeared after the second week.
- 3.3. The initially high FOM at temperatures of 30 or 36°C and the low FOM at 18 or 12°C changed over 4 weeks to approach the FOM of fish at 24°C.
- 4.4. This change in the relationship of FOM to temperature from highly dependent to independent appeared to be thermal compensation.
- 5.5. Heart and liver CCO activities were significantly affected by temperature, with the lowest activity at the approximate optimum temperature for growth, 24°C.
- 6.6. Highest CCO activities for heart and liver occurred at both the highest and lowest temperatures.
- 7.7. Among the three tissues, heart CCO activity was generally the highest and most affected by acclimation temperature.
- 8.8. Muscle tissue had the lowest CCO activity and was unaffected by temperature.
- 9.9. The high CCO activity at a cold acclimation of temperature 12°C was probably due to thermal compensation and the high activity at 36°C may have been a result of thermal stress.
15.
《The International journal of biochemistry》1993,25(2):247-251
- 1.1. Neonatal mice received subcutaneous injections of buffer, thiourea (TU) or propylthiouracil (PTU).
- 2.2. The PTU-treated mice were sacrificed on postnatal day 14 (P14) and the TU-treated mice on P28.
- 3.3. Brain weights of the TU- and PTU-treated mice were not significantly different from the controls.
- 4.4. Acid but not alkaline phosphatase activity in the braistem decreased after TU and PTU treatment.
- 5.5. Myelination as indicated by intensity of luxol fast blue staining was weaker in drug-treated animals.
- 6.6. The level of myelin marker enzyme, 2′,3′-cyclic nucleotide 3′-phosphohydrolase, was lower in the brainstem of PTU-treated animals.
- 7.7. The results suggest a correlation between acid phosphatase but not alkaline phosphatase activity with myelination in the developing mouse brain.
16.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(3):569-571
- 1.1. The influence of the gut microflora on lipid metabolism was investigated in germ-free (GF) and conventional (CV) laying Japanese quail.
- 2.2. Serum and egg yolk cholesterol concentrations showed comparable values in both GF and CV environments.
- 3.3. The fatty acid compostion of liver lipids was modified by the presence of gut microflora. Notably, in the presence of the gut microflora, proportion of oleic acid was reduced and conversely, stearic and linoleic acids were enhanced.
- 4.4. In egg yolk lipids, the proportion of myristoleic and palmitoleic acids was significantly lowered and that of stearic acid was significantly enhanced by the presence of the gut microflora, though the difference was very small.
- 5.5. It was suggested that oleic acid could be easily either hydrogenated to stearic acid or desaturated to linoleic acid by the action of the gut microflora in Japanese quail.
17.
On the synthesis and incorporation of catalase and urate oxidase into the peroxisomes of mouse liver
《The International journal of biochemistry》1983,15(12):1429-1437
- 1.1. The processes associated with the biogenesis of peroxisomes in mouse liver have been studied by following the incorporation of radiolabelled leucine into major enzymic components of this organelle.
- 2.2. Maximal incorporation of label into peroxisomal catalase and urate oxidase occurred within 2 hr, with the urate oxidase being labelled before catalase, but subsequent to the incorporation of phospholipid into this organelle.
- 3.3. Subsequently, immunoprecipitation of catalase from the large granular fraction of mouse liver was shown to result in the isolation of a catalase molecule which had lost a peptide of approx. 2000 dalton from each subunit by comparison with the newly-synthesized enzyme.
- 4.4. It was observed that the modification of catalase was obviated by the presence of leupeptin and iodoacetamide and this information has enabled the purification of both modified and unmodified forms of the enzyme.
- 5.5. The possible significance of these data has been discussed and the major features incorporated into a working model of peroxisomal biogenesis.
18.
Summary Changes of the specific activity of 3-mercaptopyruvate sulfurtransferase (MPST), rhodanese and cystathionase in Ehrlich ascites tumor cells (EATC) and tumor-bearing mouse liver after intraperitoneal administration of thiazolidine derivatives, L-cysteine, D,L-methionine, thiocystine or thiosulfate were estimated. Thiazolidine derivatives used were: thiazolidine-4-carboxylic acid (CF), 2-methyl-thiazolidine-2,4-dicarboxylic acid (CP) and 2-methyl-thiazolidine-4-carboxylic acid (CA). In the liver, the activity of MPST was significantly increased by all the studied compounds, whereas the activity of rhodanese was by CF and thiocystine and that of cystathionase was by the administration of cysteine and CP. Un the other hand, cysteine lowered the rhodanese activity and the activity of cystathionase was decreased by the administration of methionine and thiocystine. Activities of MPST and rhodanese were even lower in EATC than those in the liver of tumor-bearing mouse and the activity of cystathionase in EATC was not be detected. The thiazolidine derivatives significantly increased the level of MPST activity in EATC, but decreased the rhodanese activity. Thiosulfate also increased the activity of MPST to a lesser degree, but cysteine, methionine and thiocystine gave little change in the activity. The rhodanese activity in EATC was slightly increased only by thiocystine. These findings suggest that the sulfur metabolism in the tumor-bearing mouse liver is different from that in the normal mouse liver, and that sulfur compounds are minimally metabolized to sulfane sulfur, a labile sulfur, in EATC. 相似文献
19.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1981,68(3):595-599
- 1.1. Purine nucleoside kinase activities (adenosine kinase, deoxyadenosine kinase and arabinosyl adenine kinase) in mouse tissues were in the following order: liver > kidney > heart > lung > brain > spleen > intestine.
- 2.2. Ratios of deoxyadenosine or arabinosyl adenine kinase to adenosine kinase were significantly higher (10–200 fold) in human lymphoid cells than in mouse tissues.
- 3.3. Leukocytes from T-cell acute lymphoblastic leukemic patients had 5–20 fold elevated adenosine deaminase as compared to normal T-cells.
20.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(4):747-755
- 1.1. A comparison was made of the mechanical performance of heart muscle from mouse, an atricial mammal, with corticosterone as glucocorticoid and spiny mouse (Acomys cahirinus), a precocial mammal, with cortisol as glucocorticoid.
- 2.2. Force-frequency responses were negative in mouse and positive in spiny mouse.
- 3.3. During recovery, there was a gradual increase and an overshoot in the mouse, while in the spiny mouse there was an initial enhanced response, diminishing gradually with time.
- 4.4. High calcium concentration inhibited contractile tension in mouse heart, while it was positively inotropic in spiny mouse heart. Changes in the concentration of calcium did not change the patterns of force-frequency response.
- 5.5. Lowering the experimental temperature increased the time course and amplitude of the tension curve. However, various parameters exhibited different temperature sensitivity.
- 6.6. There was a significant difference in the levels of circulating cortisol between male and female spiny mice.
- 7.7. It is proposed that the differences in the mechanical responses of mouse and spiny mouse hearts may be explained in terms of the effects of the specific glucocorticoid hormone on the development of the sodium-calcium exchanger.