Development of cyclic AMP receptor protein-based artificial transcription factor for intensifying gene expression

Applied Microbiology and Biotechnology - Vector-dependent gene overexpression typically relies on an efficient operon and sufficient RNA polymerases (RNAPs). The lac (lactose) operon is a paradigm...     

Insulin control of cyclic AMP phosphodiesterase

Cyclic AMP phosphodiesterase (PDE) is an enzyme involved in cellular homeostasis of cyclic AMP. It exists as multiple isozymes in cells, but only the high affinity, membrane-bound isozyme is sensitive to hormonal modulation. Several isozymes or isoforms of the low Km PDE have been detected. Data suggest that several mechanisms exist for hormonal modulation of PDE. Activity of the low Km PDE species may be modulated by phosphorylation/dephosphorylation, phospholipid substrate concentration, insulin second messenger, cyclic GMP, guanine nucleotide binding proteins, calmodulin, or aggregation/disaggregation of monomeric forms. Modulation of PDE isoforms by different hormones may be through different regulatory components or mechanisms.     

Control of developmental transitions in the cyclic AMP signalling system of Dictyostelium discoideum

In the first few hours after starvation, the developing cAMP secretory system in Dictyostelium discoideum has been observed to be successively in one of four states: (a) quiescent, (b) excitable (capable of relay), (c) autonomously oscillating, and (d) secreting at a high steady level. A theoretical model is presented which demonstrates that the proximal cause of the transitions between different types of behavior may be slow changes in the activities of the enzymes adenylate cyclase and phosphodiesterase. These changes affect the stability properties of the steady state admitted by the cAMP signalling system. Sustained oscillations develop when the steady state is unstable, whereas relay of cAMP signals occurs upon perturbation of a stable steady state for parameter values close to those which produce oscillations. The developmental path suggested in the adenylate cyclase-phosphodiesterase space for the sequential transitions compares with the time course observed for the synthesis of these enzymes after starvation. It is suggested that there is general significance for the understanding of differentiation in the example given of a state-point following a developmental path in parameter space, moving from one behavioral domain to another, and thereby bringing about shifts in qualitative behavior.