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1.
V. M. Kaluskar B. P. Kapadanis M. J. Penninckx 《World journal of microbiology & biotechnology》1995,11(3):353-354
A Streptomyces sp. isolate, from decayed wood shavings, solubilized lignocellulose (LC) and lignin of Pinus radiata, producing about 50 mg acid-precipitable polymeric lignin per g LC. The product was poor in protein and carbohydrates and contained mainly vanillin, guaicol, vanillic and ferulic acids. Hardwood LC is thus suitable for producing APPL as a phenolic chemical feedstock.V.M. Kaluskar is with the Department of Microbiology, J and J Science College, Nadiad 387001, Gujarat, India. B.P. Kapadanis is with the Department of Microbiology, School of Sciences, University of Pune, Ganesh Khind, Pune-41107, Maharashtra, India. M.J. Penninckx is with the Unit of Microbial Physiology and Ecology, Free University of Brussels, c/o IPB 642, rue Engeland, B-1180, Brussels, Belgium 相似文献
2.
Pavel Petrosyan Agustín Luz-Madrigal Carlos Huitrón María Elena Flores 《Biotechnology letters》2002,24(18):1473-1476
Streptomyces sp. DSM 41796 produced four major extracellular xylanases with Mr of 145, 120, 60 and 45 kDa. Those of 145 and 60 kDa formed a heterodimer. All xylanases, except that of 120 kDa, were induced by xylose, d-arabinose or sucrose, while commercial xylans induced the 60 kDa xylanase in a major proportion than others, and sugar-cane bagasse pith or lemon peel induced predominantly the 45 kDa xylanase. 相似文献
3.
A Streptomyces sp. was isolated that produced novel thermoalkalotolerant cellulase activity after growth on crystalline cellulose at 50°C.
Three major components of the cellulases (CMCase, Avicelase and cellobiase) were produced with maximal activities (11.8, 7.8
and 3.9 IU/ml) and maximum specific activities 357, 276 and 118 IU/mg protein, respectively, after 120 h growth. Maximum CMCase
activity was between 50 and 60°C measured over 3 h. The enzyme also retained 88% of its maximum activity at 70°C and pH 5,
and 80% of the activity at pH 10 and 50°C when assayed after 1 h. After incubation at 40°C for 1 h with commercial detergent
(Tide) at pH 11, 95% activity was retained. The enzyme mixture produced glucose from crystalline cellulose. 相似文献
4.
Sacramento Doralice Rodrigues Coelho Rosalie Reed Rodrigues Wigg Márcia Dutra Toledo Luna Linhares Luiz Fernando de Matos dos Santos Marta Gonçalves Azevedo Soares Semêdo Luzia Teixeira de Ribeiro da Silva Antonio Jorge 《World journal of microbiology & biotechnology》2004,20(3):225-229
A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial
activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions,
obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic
fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type
1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
5.
Shin Hye Park Jung-Hyun Lee Sung-Hwan Ko Deuk-Soo Lee Hong Kum Lee 《Biotechnology letters》2000,22(17):1389-1395
Of a number of actinomycetes isolated from Antarctica and coastal regions of Korea, one isolate, identified as a Streptomycessp., demulsified an emulsion of kerosene/0.2% Triton X-100 (2:1, v/v) within 1 min. The aerial spores broke emulsions of low viscosity hydrocarbons such as kerosene and gasoline within 1 min, but after 3 min of contact they could demulsify less than 50% of emulsions of high viscosity hydrocarbons diluted with kerosene. 相似文献
6.
Sergio A. Cuozzo Graciela G. Rollán Carlos M. Abate María J. Amoroso 《World journal of microbiology & biotechnology》2009,25(9):1539-1546
Synthesis of dechlorinase in Streptomyces sp. M7 was induced when the microorganism was grown in the presence of lindane (γ-hexachlorocyclohexane) as the only carbon source. Activity of cells grown with lindane was about four and half times higher
compared to cells grown with glucose. Maximum dechlorinase activity was observed at 30°C in alkaline conditions pH (7.9) and
the enzyme did not show cation dependency. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed one differential
band with a molecular weight similar to serum albumin (M
r 66,200), which corresponded to polynucleotide phosphorylase, an enzyme that plays an important role in the regulation system
and could be involved in the regulation of the dechlorinase gene. Detected in cell-free extracts were γ-pentachlorocyclohexene and 1,3,4,6-tetrachloro-1,4-cyclohexadiene, both being products of the dechlorinase activity. This
is the first time that the presence of an enzyme with dechlorinase activity has been demonstrated in an actinomycete strain
isolated in Tucumán, Argentina. Characteristics of this enzyme revealed that Streptomyces sp. M7 could be useful in the future in bioremediation of soil or as a biosensor. 相似文献
7.
Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp. 总被引:1,自引:0,他引:1
Charin Techapun Suphawat Sinsuwongwat Naiyatat Poosaran Masanori Watanabe Ken Sasaki 《Biotechnology letters》2001,23(20):1685-1689
1444 microorganisms were isolated from soil samples from the northern Thai and screened at 55 °C by using basal medium supplemented with 1% carboxymethyl cellulose as a sole carbon source. One isolate, Streptomyces Ab106, had a high activity of a cellulase-free xylanase also without mannanase activity. The maximum cellulase-free xylanase activities of 3.5, 3.3, 3.1 and 2.7 IU were after growth of the organism with 1% (w/v) corn hull, corncob, bagasse and oat spelt xylan, respectively, at 55 °C for 6 days, respectively. The activity was more than 5 times higher than that at 35 °C. 相似文献
8.
Manfio GP Atalan E Zakrzewska-Czerwinska J Mordarski M Rodríguez C Collins MD Goodfellow M 《Antonie van Leeuwenhoek》2003,83(3):245-255
The taxonomic positions of soil isolates known as Streptomyces groups A, B and C were clarified. Comparative 16S rDNA sequence studies indicated that representatives of all three taxa
formed distinct phyletic lines within the Streptomyces tree though the group A strains were shown to be related to Streptomyces griseus and associated validly described species. The taxonomic integrity of all three groups was highlighted by DNA:DNA relatedness
and ribotype data though the group A strains encompassed a higher degree of genetic variation than the group B and C strains.
In light of these and earlier phenotypic data it is proposed that Streptomyces groups A, B and C be given species status as Streptomyces sanglieri sp. nov., Streptomyces aureus sp. nov. and Streptomyces laceyi sp. nov., respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
Glutamate oxidase activity was studied in 1254Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers
of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis
were optimized. A multistep mutagenesis-selection procedure allowed a genetically stable strain,Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate. 相似文献
10.
Bioconversion of compactin into pravastatin by Streptomyces sp. 总被引:3,自引:0,他引:3
Park JW Lee JK Kwon TJ Yi DH Kim YJ Moon SH Suh HH Kang SM Park YI 《Biotechnology letters》2003,25(21):1827-1831
Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l–1 from 750 mg compactin l–1 in 24 h. By intermittent feeding of compactin into the culture medium, both the compactin concentration and its conversion increased to 2000 mg l–1 and 1000 mg pravastatin l–1, respectively, with the conversion rate of 10 mg l–1 h–1. Continuous feeding of compactin increased production of pravastatin to 15 mg l–1 h–1. 相似文献
11.
A growth medium based on starch and fish flour, optimal for the inducible synthesis of elastase by strain Streptomyces sp. 82 was composed using a factorial experiment. Elastase yield was raised 7.5 times compared to the basic medium. 相似文献
12.
The taxonomic position of an actinomycete isolated from an ultramafic soil in New Caledonia was examined using a polyphasic
approach. The organism, which was designated SFOCin 76, was found to have chemical and morphological properties typical of
streptomycetes and formed a distinct phyletic line in the Streptomyces violaceusniger clade of the 16S rDNA tree. It also showed a unique pattern of phenotypic properties that distinguished it from representatives
of all of the validly described species classified in this clade. It is, therefore, proposed that strain SFOCin 76 be classified
in the genus Streptomyces as Streptomyces yatensis sp. nov.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
13.
A novel actinomycete strain YIM 31724T was isolated from a soil sample collected from Dali, Yunnan Province, People’s Republic of China. The strain is characterized
by white to yellow white aerial mycelia, spiral spore chains and smooth spore surface. The cell wall of strain YIM 31724T contained LL-diaminopimelic acid (A2pm) and traces of meso-A2pm. Whole-cell hydrolysates contained mainly glucose and small amounts of galactose and xylose. The menaquinones were MK-9(H6) (31%) and MK-9(H8) (69%). Phosphatidylethanolamine was the diagnostic phospholipid. The DNA G+C content of strain YIM 31724T was 67.2 mol%. Phylogenetic analysis indicated that the strain belongs to the genus Streptomyces, with highest similarity to Streptomyces rimosus subsp. rimosus JCM 4667T (rRNA gene sequence similarity value of 98.9%) and Streptomyces erumpens DSM 40941T (rRNA gene sequence similarity value of 98.7%). Based on its phenotypic and genotypic characteristics, including low DNA–DNA
hybridization results, strain
is proposed as the type strain of a novel species, Streptomyces daliensis sp. nov. 相似文献
14.
Syed DG Agasar D Kim CJ Li WJ Lee JC Park DJ Xu LH Tian XP Jiang CL 《Antonie van Leeuwenhoek》2007,92(4):391-397
A Gram-positive, nonmotile, moderately halophilic, alkali and thermotolerant strain designated DAS 165(T), was isolated from a dry land soil sample from the Gulbarga region, Karnataka province, India. The isolate produced yellow substrate mycelia and gray aerial mycelia on most tested media. Strain DAS 165(T) showed growth in the presence of 5 to 7% NaCl and at 45 degrees C. The DNA G + C content was 69.7%. 16S rRNA gene sequence analysis together with these characteristics consistently assigned strain DAS 165(T) to the genus Streptomyces. The 16S rRNA gene sequence analysis revealed that strain DAS 165(T) was most closely related to S. tendae ATCC 19812(T) (D 63873) with a sequence similarity of 99.6% (three nucleotide differences out of 1,517). Strain DAS 165(T) formed a distinct clade based on analysis of the almost complete sequence and 120-nucleotide variable gamma region of the 16S rRNA gene. Despite the high sequence similarity, strain DAS 165(T) was phenotypically different from S. tendae ATCC 19812(T). DNA-DNA hybridization between these strains was 47% showing that strain DAS 165(T) is a distinct genomic species. Phenetic and genetic results support the classification of strain DAS 165(T) as a new species, for which the name S. tritolerans is proposed, with strain DAS 165(T) as the type strain (=DSM 41899(T )= CCTCCAA 206013(T)). 相似文献
15.
Masaru Kitagawa Tomoo Tokiwa Hong Fan Takao Raku Yutaka Tokiwa 《Biotechnology letters》2000,22(10):879-882
The transesterification of 1 M divinyladipate with 0.25 M glucose in dimethylformamide (DMF) catalyzed by 5 mg ml–1 alkaline protease (24 units mg–1 min–1) from Streptomyces sp. gave 6-O-vinyladipoyl d-glucose as the main product with yields are between 60 and 90%. The optimum temperature for the reaction was about 50 °C. 相似文献
16.
The survival of two different pentachlorophenol (PCP)-degrading bacteria were studied in natural soil. The PCP-degraders Rhodococcus chlorophenolicus and Flavobacterium sp., both able to mineralize PCP into CO2 and chloride in axenic culture, were tested for the capacity to survive and degrade PCP in natural soil. These bacteria were immobilized on polyurethane (PUR) foam and introduced into natural peaty soil to give about 109 cells g-1 of soil (dry weight). R. chlorophenolicus induced PCP-degrading activity in soil remained detectable for 200 days whether or not a carbon source was added (distillery waste or wood chips). Electron microscopic investigation performed almost a year after inoculation, revealed the presence of R. chlorophenolicus-like cells in the PUR foam particles. PCP-degrading activity of Flavobacterium sp. declined within 60 days of burial in the soil without enhancing the PCP removal. R. chlorophenolicus degraded PCP in soil at a mean rate of 3.7 mg of PCP day-1 kg-1 of soil, which corresponds to ca. 5×10-3 pg of PCP degraded per inoculated R. chlorophenolicus cell day-1. The solvent extractable organic chlorine contents of the soil decreased stoichiometrically (>95%) with that of PCP indicating that PCP was essentially mineralized.Abbreviations ATCC
American type culture collection
- DSM
Deutsche Sammlung für Mikroorganismen
- DW
distillery waste
- EM
electron microscopy
- EOX
extractable organic halogen
- GC/ECD
gas chromatograph/electron capture detector
- GC/MS
gas chromatograph/mass spectrometer
- PCP
pentachlorophenol
- WC
wood chips
- d.wt.
dry weight
- w.wt.
wet weight
- d.s.
dry soil
- d.H2O
distilled water
- PCA
polychlorinated aromatics 相似文献
17.
Sivakumar U. Kalaichelvan G. Ramasamy K. 《World journal of microbiology & biotechnology》2004,20(6):563-568
Biodegradation of lignin by Streptomyces spp. results in the production of value-added chemicals such as Acid Precipitable Polymeric Lignins (APPLs), low molecular
weight phenols, etc. To hasten the conversion metabolism through genetic manipulation, a preliminary attempt was made to standardize
the methodology for isolation and regeneration of protoplasts. Protoplast fusion recombinants were developed and assayed for
their ligninolytic activity, production of ligninolytic enzymes viz., peroxidase, laccase, polyphenol oxidase and crude protein. In comparison with the mutants and wild strain, fusion recombinant
F4 showed higher laccase activity and lower peroxidase activity. This attribute can be positively used for polymerization of
free phenolics to polycondensates related to humic acids in soil or composting environments.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
18.
Cloning and Characterization of Alginate Lyase from a Marine Bacterium Streptomyces sp. ALG-5 总被引:2,自引:0,他引:2
A marine bacterium was isolated from seaweeds for its ability to degrade alginate. Analysis of 16S ribosomal DNA sequence
and chemotaxonomic characterizations revealed that the strain belongs to Streptomyces. The alginate lyase gene of Streptomyces sp. ALG-5 was cloned by using PCR with the specific primer designed from homologous nucleotide sequences. The consensus sequences
of N-terminal YXRSELREM and C-terminal YFKAGXYXQ were conserved in the ALG-5 alginate lyase gene. The recombinant alginate
lyase was purified by using Ni-Sepharose affinity chromatography. The alginate lyase appears to be poly-guluronate lyase degrading
poly-G block preferentially than poly-M block. The degraded products were determined to be di-, tri-, tetra- and pentasaccharides
by using BioGel P-2 gel filtration chromatography and ionization mass spectroscopy method. 相似文献
19.
Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9 总被引:1,自引:1,他引:0
Li N Meng K Wang Y Shi P Luo H Bai Y Yang P Yao B 《Applied microbiology and biotechnology》2008,80(2):231-240
A new xylanase gene, xynAS9, was cloned from Streptomyces sp. S9, which was isolated from Turpan Basin, China. The full-length gene consists of 1,395 bp and encodes 465 amino acids including 38 residues of a putative signal peptide. The overall amino acid sequence shares the highest identity (50.8%) with a putative endo-1,4-beta-xylanase from Streptomyces avermitilis of the glycoside hydrolase family 10. The gene fragment encoding the mature xylanase was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified to electrophoretic homogeneity and subsequently characterized. The optimal pH and temperature for the recombinant enzyme were 6.5 and 60 degrees C, respectively. The enzyme showed broad temperature adaptability, retaining more than 65% of the maximum activity when assayed at 50-80 degrees C. The enzyme also had good thermal and pH stability. The K (m) values for oat spelt xylan and birchwood xylan substrates were 2.85 and 2.43 mg ml(-1), with the V (max) values of 772.20 and 490.87 mumol min(-1) mg(-1), respectively. The hydrolysis products of xylan were mainly xylose and xylobiose. These favorable properties should make XynAS9 a good candidate in various industrial applications. 相似文献
20.
Effect of amino acids on production of xylanase and pectinase from Streptomyces sp. QG-11-3 总被引:5,自引:0,他引:5
Qasim Khalil Beg Bharat Bhushan Mukesh Kapoor G.S. Hoondal 《World journal of microbiology & biotechnology》2000,16(2):211-213
Xylanase and pectinase production by Streptomyces sp. QG-11-3 was stimulated by DL-norleucine, L-leucine, DL-isoleucine, L-lysine monohydrochloride and DL--phenylalanine by up to 3.72- and 2.78-fold, respectively, whereas the combination of DL-norleucine, L-leucine and DL-isoleucine synergistically stimulated the xylanase and pectinase production by up to 6.72- and 5.62-fold, respectively. Glycine, DL-norvaline, DL-methionine, and DL-aspartic acid showed no significant stimulatory effect on enzyme production. 相似文献