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1.
In biological oceanography, it has been widely accepted that the maximum quantum yield of photosynthesis is influenced by nutrient stress. A closely related parameter, the maximum quantum yield for stable charge separation of PSII, (φ PSII )m, can be estimated by measuring the increase in fluorescence yield from dark-adapted minimal fluorescence (Fo) to maximal fluorescence (Fm) associated with the closing of photosynthetic reaction centers with saturating light or with a photosynthetic inhibitor such as 3′-(3,4-dichlorophenyl)-1′,1′-dimethyl urea (DCMU). The ratio Fv/Fm (= (Fm− Fo)/Fm) is thus used as a diagnostic of nutrient stress. Published results indicate that Fv/Fm is depressed for nutrient-stressed phytoplankton, both during nutrient starvation (unbalanced growth) and acclimated nutrient limitation (steady-state or balanced growth). In contrast to published results, fluorescence measurements from our laboratory indicate that Fv/Fm is high and insensitive to nutrient limitation for cultures in steady state under a wide range of relative growth rates and irradiance levels. This discrepancy between results could be attributed to differences in measurement systems or to differences in growth conditions. To resolve the uncertainty about Fv/Fm as a diagnostic of nutrient stress, we grew the neritic diatom Thalassiosira pseudonana (Hustedt) Hasle et Heimdal under nutrient-replete and nutrient-stressed conditions, using replicate semicontinuous, batch, and continuous cultures. Fv/Fm was determined using a conventional fluorometer and DCMU and with a pulse amplitude modulated (PAM) fluorometer. Reduction of excitation irradiance in the conventional fluorometer eliminated overestimation of Fo in the DCMU methodology for cultures grown at lower light levels, and for a large range of growth conditions there was a strong correlation between the measurements of Fv/Fm with DCMU and PAM (r2 = 0.77, n = 460). Consistent with the literature, nutrient-replete cultures showed consistently high Fv/Fm (∼0.65), independent of growth irradiance. Under nutrient-starved (batch culture and perturbed steady state) conditions, Fv/Fm was significantly correlated to time without the limiting nutrient and to nutrient-limited growth rate before starvation. In contrast to published results, our continuous culture experiments showed that Fv/Fm was not a good measure of nutrient limitation under balanced growth conditions and remained constant (∼0.65) and independent of nutrient-limited growth rate under different irradiance levels. Because variable fluorescence can only be used as a diagnostic for nutrient-starved unbalanced growth conditions, a robust measure of nutrient stressed oceanic waters is still required. 相似文献
2.
1. Non‐indigenous ecosystem engineers can substantially affect native biodiversity by transforming the physical structure of habitats. In the Great Lakes–St. Lawrence River system, introduced dreissenid mussels (Dreissena polymorpha and D. bugensis) and the native benthic macroalga Cladophora act as ecosystem engineers by increasing substratum complexity and providing interstitial habitat for benthic macroinvertebrates. 2. We manipulated the topography and perimeter‐to‐area ratio of patches of dreissenid mussels in a series of colonisation experiments conducted at two sites in the St. Lawrence River. Experimental substrata were variably colonised by Cladophora, prompting us to examine (i) how the topography of Dreissena patches affects benthic macroinvertebrate diversity and (ii) the extent to which the effects of Dreissena are altered by the presence of another habitat‐modifying organism (Cladophora). 3. The results of our first experiment suggested that a patchy distribution of dreissenid mussels is an important driver of benthic diversity at small spatial scales. The results of our second and third experiments suggested that a native habitat engineer, Cladophora, modifies the impact of Dreissena on benthic macroinvertebrate communities. 4. While macroalgal blooms have been linked to the large‐scale impacts of Dreissena on light and nutrient availability, Dreissena shells inhibited Cladophora growth at our experimental scale. These findings demonstrate that the interactions between habitat‐modifying species can complicate efforts to predict the community‐level effects of an invasion. 相似文献
3.
Blake A. Schaeffer Daniel Kamykowski Laurie McKay Geoff Sinclair Edward J. Milligan 《Journal of phycology》2007,43(4):702-714
Many laboratories have solely used the Wilson isolate to physiologically characterize the harmful algal bloom (HAB) dinoflagellate Karenia brevis (C. C. Davis) G. Hansen et Moestrup. However, analysis of one isolate may lead to misinterpretations when extrapolating measurements to field populations. In this study, pulse‐amplitude‐modulated chlorophyll fluorometer (PAM‐FL) relative electron transport rate (ETR), Fv/Fm, and chl were compared with traditional techniques, such as 14C photosynthesis versus irradiance (P–E) curves, DCMU [3‐(3′,4′‐dichlorophenyl)‐1,1‐dimethyl urea] Fv/Fm, and extracted chl. The DCMU and PAM‐FL values of Fv/Fm (r2 = 0.51) and chl (r2 = 0.58) were in good agreement. There was no correlation between 14C and PAM‐FL α, Pmax, and β parameters because PAM‐FL ETR was only a relative measurement. The PAM‐FL techniques were then used to investigate P–E curves, quantum yield of PSII (Fv/Fm), and chl from 10 K. brevis isolates to determine whether one or all isolates would better represent the species. Comparisons were made with a radial photosynthetron, which allowed for controlled conditions of light and temperature. Isolate α, Pmax, and β varied between 0.097 and 0.204 μmol e? · m?2 · s?1 · (μmol quanta · m?2 · s?1)?1, 80.41 and 241 μmol e? · m?2 · s?1, and 0.005 and 0.160 μmol e? · m?2 · s?1 · (μmol quanta · m?2 · s?1)?1, respectively. Either carbon limitation and/or bacterial negative feedback were implicated as the cause of the P–E parameter variability. Furthermore, these results directly contradicted some literature suggestions that K. brevis is a low‐light‐adapted dinoflagellate. Results showed that K. brevis was more than capable of utilizing and surviving in light conditions that may be present on cloudless days off Florida. 相似文献
4.
5.
1. Our goal was to use physiological indicators [photosynthesis–irradiance (P–I) response, nutrient status], population level feedbacks (self‐shading) and ambient environmental conditions (dissolved nutrients, light, temperature) to improve our understanding of the seasonal and spatial population dynamics of Cladophora. 2. Cladophora grew in three distinct phases, rapid growth early in the season (May–July), a mid‐season population collapse (July–August) and autumn re‐growth. Across all sites and dates, mean net maximal photosynthesis [PM (NET)] was 6.9 ± 3.9 mg O2 g DM?1 h?1, and α was 0.055 ± 0.025 mg O2 g DM?1 μm photons?1 m?2. Mean values for critical irradiance (ICR) and the half‐saturation light intensity (IK), were 42.9 ± 32.1 and 189.3 ± 123.8 μm photons?1 m?2 s?1 respectively. 3. At most sites growth was phosphorus‐limited. Values of α were significantly higher at a site influenced by a nutrient enriched river plume, where algal growth was phosphorus‐sufficient. 4. Photoinhibition was not apparent in any of our P–I experiments. Even if photoinhibition had been apparent during in vitro P–I experiments, population level photosynthetic rates in the field would be little affected because intense self‐shading restricts inhibiting irradiances to the upper few mm–cm of the algal canopy. 5. Our physiological (P–I response) experiments contradicted previous assertions that high ambient temperatures, or nutrient deficiency, were primary causes of mid‐summer sloughing. In our study, sloughing occurred simultaneously at nutrient enriched and nutrient deficient sites, at temperatures well below critical values found during in vitro experiments, and our indicator of physiological condition (P–I response) remained unchanged leading up to, or immediately after, the sloughing event. 6. Self‐shading can reduce the convexity of the P–I response within in vitro incubations, even when the amount of algal material is low. Our experiments used 0.08 g DM of algal material that formed clumps c. 1 cm thick. Under these conditions, we estimated negligible (<1%) effects on PM, a 12% reduction in apparent values of α, and 14% and 17% increases in values of the α‐dependent terms ICR and IK, respectively. 7. Our results are consistent with the hypothesis that a population‐level negative feedback (self‐shading) is responsible for sloughing in dense macroalgal beds. Sloughing was probably inevitable once macroalgal bed density and thickness surpassed a critical threshold. Cells towards the base of the bed received insufficient light to maintain metabolic balance, began to decay and weaken, and became increasingly susceptible to physical detachment from shear stress. 相似文献
6.
Elizabeth C. Cooney Kerri A. Fredrickson Kelley J. Bright Suzanne L. Strom 《Journal of phycology》2019,55(5):1082-1095
While light limitation can inhibit bloom formation in dinoflagellates, the potential for high‐intensity photosynthetically active radiation (PAR) to inhibit blooms by causing stress or damage has not been well‐studied. We measured the effects of high‐intensity PAR on the bloom‐forming dinoflagellates Alexandrium fundyense and Heterocapsa rotundata. Various physiological parameters (photosynthetic efficiency Fv/Fm, cell permeability, dimethylsulfoniopropionate [DMSP], cell volume, and chlorophyll‐a content) were measured before and after exposure to high‐intensity natural sunlight in short‐term light stress experiments. In addition, photosynthesis‐irradiance (P‐E) responses were compared for cells grown at different light levels to assess the capacity for photophysiological acclimation in each species. Experiments revealed distinct species‐specific responses to high PAR. While high light decreased Fv/Fm in both species, A. fundyense showed little additional evidence of light stress in short‐term experiments, although increased membrane permeability and intracellular DMSP indicated a response to handling. P‐E responses further indicated a high light‐adapted species with Chl‐a inversely proportional to growth irradiance and no evidence of photoinhibition; reduced maximum per‐cell photosynthesis rates suggest a trade‐off between photoprotection and C fixation in high light‐acclimated cells. Heterocapsa rotundata cells, in contrast, swelled in response to high light and sometimes lysed in short‐term experiments, releasing DMSP. P‐E responses confirmed a low light‐adapted species with high photosynthetic efficiencies associated with trade‐offs in the form of substantial photoinhibition and a lack of plasticity in Chl‐a content. These contrasting responses illustrate that high light constrains dinoflagellate community composition through species‐specific stress effects, with consequences for bloom formation and ecological interactions within the plankton. 相似文献
7.
Ryuta Terada Yuto Nakashima Iris Ann Borlongan Hiromori Shimabukuro Jumpei Kozono Hikaru Endo Satoshi Shimada Gregory N. Nishihara 《Phycological Research》2020,68(1):70-79
The effects of irradiance, temperature, thermal‐ and chilling‐light sensitivities on the photosynthesis of a temperate alga, Sargassum macrocarpum (Fucales) were determined by a pulse amplitude modulation (PAM)‐chlorophyll fluorometer and dissolved oxygen sensors. Oxygenic photosynthesis–irradiance curves at 8, 20, and 28°C revealed that the maximum net photosynthetic rates (NP max) and saturation irradiance were highest at 28°C, and lowest at 8°C. Gross photosynthesis and dark respiration determined over a range of temperatures (8–36°C) at 300 μmol photons m?2 s?1 revealed that the maximum gross photosynthetic rate (GPmax) occurred at 27.8°C, which is consistent with the highest seawater temperature in the southern distributional limit of this species in Japan. Additionally, the maximum quantum yields of photosystem II (F v/F m) during the 72‐h temperature exposures were stable at 8–28°C, but suddenly dropped to zero at higher temperatures, indicative of PSII deactivation. Continuous exposure (12 h) to irradiance of 200 (low) and 1000 (high) μmol photons m?2 s?1 at 8, 20, and 28°C revealed greater declines in their effective quantum yields (Φ PSII) under high irradiance. While Φ PSII under low irradiance were very similar with the initial F v/F m under 20 and 28°C, values rapidly decreased with exposure duration at 8°C. At this temperature, F v/F m did not recover to initial values even after 12 h of dark acclimation. Final F v/F m of alga at 28°C under high irradiance treatment also did not recover, suggesting its sensitivity to photoinhibition at both low and high temperatures. These photosynthetic characteristics reflect both the adaptation of the species to the general environmental conditions, and its ability to acclimate to seasonal changes in seawater temperature within their geographical range of distribution. 相似文献
8.
Matthew E. S. Bracken 《Journal of phycology》2004,40(6):1032-1041
Even in nitrogen‐replete ecosystems, microhabitats exist where local‐scale nutrient limitation occurs. For example, coastal waters of the northeastern Pacific Ocean are characterized by high nitrate concentrations associated with upwelling. However, macroalgae living in high‐zone tide pools on adjacent rocky shores are isolated from this upwelled nitrate for extended periods of time, leading to nutrient limitation. When high‐intertidal pools are isolated during low tide, invertebrate‐excreted ammonium accumulates, providing a potential nitrogen source for macroalgae. I quantified the influence of mussels (Mytilus californianus Conrad) on ammonium accumulation rates in tide pools. I then evaluated the effects of ammonium loading by mussels on nitrogen assimilation and growth rates of Odonthalia floccosa (Esp.) Falkenb., a common red algal inhabitant of pools on northeastern Pacific rocky shores. Odonthalia was grown in artificial tide pool mesocosms in the presence and absence of mussels. Mesocosms were subjected to a simulated tidal cycle mimicking emersion and immersion patterns of high‐intertidal pools on the central Oregon coast. In the presence of mussels, ammonium accumulated more quickly in the mesocosms, resulting in increased rates of nitrogen assimilation into algal tissues. These increased nitrogen assimilation rates were primarily associated with higher growth rates. In mesocosms containing mussels, Odonthalia individuals added 41% more biomass than in mesocosms without mussels. This direct positive effect of mussels on macroalgal biomass represents an often overlooked interaction between macroalgae and invertebrates. In nutrient‐limited microhabitats, such as high‐intertidal pools, invertebrate‐excreted ammonium is likely an important local‐scale contributor to macroalgal productivity. 相似文献
9.
Two methods of measuring primary production, modulated fluorimetry (PAM) and the traditional carbon incorporation method (13C), were compared in four phytoplankton species, two diatoms (Pseudo-nitzschia pungens and Asterionellopsis glacialis), and two dinoflagellates (Heterocapsa sp and Karenia mikimotoï), under N (nitrogen), P (phosphorus) and Si (silicon) limited semi-continuous culture. N and Si-limited cultures showed relatively high quantum efficiency of the PSII (Fv/Fm) values, confirming that Fv/Fm is not a good proxy for nutrient stress in balanced systems, whereas P limitation had a drastic effect on many physiological parameters. In all species, the physiological capacity of phytoplankton cells to acclimate to nutrient limitations led to changes in the cellular biochemical composition and the structure of the photosynthetic apparatus. The observed physiological responses were species and nutrient specific. The values of the chlorophyll-specific absorption cross section (a*) increased with nutrient limitation due to package effect, while the carbon/Chl a ratio was higher under N and P limitations. In diatoms, Si limitation did not affect photosynthesis confirming the uncoupling between Si and carbon metabolisms. In all four species and under all treatments, significant relationships were found between photosynthetic activities, ETRChl (electron transport rate) and PChl (carbon fixation rate) estimated using PAM measurements and 13C incorporation, showing that the fluorescence technique can reliably be used to estimate carbon fixation by phytoplankton. The relationship between ETRChl and PChl can be described by the shape and the slope of the curve (ΦC.e). Linear relationships were found for dinoflagellates and P. pungens under all treatments. A decrease in ΦC.e was observed under N and P limitation probably due to structural damage to the photosynthetic apparatus. A. glacialis showed a logarithmic relationship in N and P limited conditions, due to the alternative electron flow which takes place to optimise photosynthetic performances under high light and/or nutrient stress. 相似文献
10.
Extracellular alkaline phosphatase enzyme activity (APA) is important for algal phosphorus (P) acquisition in P‐limited freshwater ecosystems and is often used as an indicator of P deficiency. APA allows access to organic P (monophosphate esters), but the regulation of APA in response to availability of both PO43− and organic P is poorly characterized. This study aimed to examine the regulation of APA in freshwater Cladophora‐epiphyte assemblages in response to PO43− and a hydrolyzable organic P source, and for the first time to apply enzyme linked fluorescence (ELF) to localize APA within freshwater macroalgal‐epiphyte assemblages. In response to elevated PO43− concentrations, a component of net APA was suppressed, but there was also a constitutive APA, which was maintained even after prolonged exposure to nearly 1,000 μM PO43− and saturation of internal P pools. When supplied with organic glycerol P as the sole P source, the algae maintained APA in excess of needs for supplying PO43− for uptake, resulting in PO43− release into the medium. Constitutive APA may be adaptive to growth under chronic P limitation in oligotrophic freshwater habitats. Excess APA and release of PO43− could benefit different algal and bacterial partners within assemblages. APA in both Cladophora sp. and epiphytic algae was localized with ELF only when ethanol fixation was omitted. In algal subsamples exposed to different P treatments, there was no correlation between bulk APA (using 4‐methylumbelliferyl phosphate [MUP] substrate) and % cell labeling with ELF, suggesting that ELF labeling of APA was at best semiquantitative in the algal assemblages. 相似文献
11.
A survey of the spatial distribution of benthic macroalgae in a fluvial lake of the St. Lawrence River (Lake Saint‐Pierre, Quebec, Canada) revealed a shift in composition from chlorophytes to cyanobacteria along the flow path of nutrient‐rich waters originating from tributaries draining farmlands. The link between this shift and changes in water quality characteristics was investigated by sampling at 10 sites along a 15 km transect. Conductivity, current, light extinction, total phosphorus (TP; >25 μg P · L?1), and ammonium (8–21 μg N · L?1) remained fairly constant along the transect in contrast to nitrate concentrations, which fell sharply. Filamentous and colonial chlorophytes [Cladophora sp. and Hydrodictyon reticulatum (L.) Bory] dominated in the first 5 km where nitrate concentrations were >240 μg N · L?1. A mixed assemblage of chlorophytes and cyanobacteria characterized a 1 km transition zone where nitrate decreased to 40–80 μg N · L?1. In the last section of the transect, nitrate concentrations dropped below 10 μg N · L?1, and cyanobacteria (benthic filamentous mats of Lyngbya wollei Farl. ex Gomont and epiphytic colonies of Gloeotrichia) dominated the benthic community. The predominance of nitrogen‐fixing, potentially toxic cyanobacteria likely resulted from excessive nutrient loads and may affect nutrient and trophic dynamics in the river. 相似文献
12.
13.
The photochemical efficiency of symbiotic dinoflagellates within the tissues of two reef‐building corals in response to normal and excess irradiance at water temperatures < 30 °C were investigated using pulse amplitude modulated (PAM) chlorophyll fluorescence techniques. Dark‐adapted Fv/Fm showed clear diurnal changes, decreasing to a low at solar noon and increasing in the afternoon. However, Fv/Fm also drifted downwards at night or in prolonged darkness, and increased rapidly during the early morning twilight. This parameter also increased when the oxygen concentration of the water holding the corals was increased. Such changes have not been described previously, and most probably reflect state transitions associated with PQ pool reduction via chlororespiration. These unusual characteristics may be a feature of an endosymbiotic environment, reflective of the well‐documented night‐time tissue hypoxia that occurs in corals. Fv/Fm decreased to 0·25 in response to full sunlight in shade‐acclimated (shade) colonies of Stylophora pistillata, which is considerably lower than in light‐acclimated (sun) colonies. In sun colonies, the reversible decrease in Fv/Fm was caused by a lowering of Fm and Fo suggesting photoprotection and no lasting damage. The decrease in Fv/Fm, however, was caused by a decrease in Fm and an increase in Fo in shade colonies suggesting photoinactivation and long‐term cumulative photoinhibition. Shade colonies rapidly lost their symbiotic algae (bleached) during exposure to full sunlight. This study is consistent with the hypothesis that excess light leads to chronic damage of symbiotic dinoflagellates and their eventual removal from reef‐building corals. It is significant that this can occur with high light conditions alone. 相似文献
14.
NPQ(T): a chlorophyll fluorescence parameter for rapid estimation and imaging of non‐photochemical quenching of excitons in photosystem‐II‐associated antenna complexes
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Stefanie Tietz Christopher C. Hall Jeffrey A. Cruz David M. Kramer 《Plant, cell & environment》2017,40(8):1243-1255
In photosynthesis, light energy is absorbed by light‐harvesting complexes and used to drive photochemistry. However, a fraction of absorbed light is lost to non‐photochemical quenching (NPQ) that reflects several important photosynthetic processes to dissipate excess energy. Currently, estimates of NPQ and its individual components (qE, qI, qZ and qT) are measured from pulse‐amplitude‐modulation (PAM) measurements of chlorophyll fluorescence yield and require measurements of the maximal yield of fluorescence in fully dark‐adapted material (Fm), when NPQ is assumed to be negligible. Unfortunately, this approach requires extensive dark acclimation, often precluding widespread or high‐throughput use, particularly under field conditions or in imaging applications, while introducing artefacts when Fm is measured in the presence of residual photodamaged centres. To address these limitations, we derived and characterized a new set of parameters, NPQ(T), and its components that can be (1) measured in a few seconds, allowing for high‐throughput and field applications; (2) does not require full relaxation of quenching processes and thus can be applied to photoinhibited materials; (3) can distinguish between NPQ and chloroplast movements; and (4) can be used to image NPQ in plants with large leaf movements. We discuss the applications benefits and caveats of both approaches. 相似文献
15.
Pulse amplitude modulated (PAM) fluorometry has been suggested as a tool for estimating environmental stresses on corals.
However, information regarding natural changes in maximal quantum yields (F
v/F
m) of corals during “normal” (i.e. non-bleaching) years has been limited. In this study, seasonal variations in F
v/F
m for Stylophora pistillata and Favia favus, measured in situ, correlated with seasonal changes in solar irradiance but not in sea temperature. Interactions between
sea temperature and irradiance were further studied by growing these corals and Pocillopora damicornis under controlled conditions. Exposure to high light with normal or high temperatures resulted in lower F
v/F
m values than exposure to low light at both temperatures. Thus, high irradiances may cause decreased F
v/F
m values in corals at least as much as, if not more than, high temperatures. Such seasonal variations should be taken into
account when using PAM fluorometry as a diagnostic tool for predicting coral bleaching. 相似文献
16.
Phytoplankton primary productivity in the Southern Ocean is controlled by complex interactions among iron, light, and grazing. This project interfaced with the Southern Ocean iron experiment (SOFeX) that created two iron‐enriched patches north and south of the Polar Front each with distinct silicic acid concentrations. We used pulse amplitude modulated fluorometry and measured the recovery of the maximum quantum yield of photochemistry (Fv/Fm) for three size fractions (whole, <5, <20 μm) and light adapted quantum yield (ΔF/F′m) for single phytoplankton cells. The rates of recovery from iron stress were found to be unrelated to average cell size for both size‐fractioned and single‐celled measurements. The smallest cells appeared to exhibit more severe iron stress at the onset of the experiment than the larger taxa. The largest response detected in regression parameters was that of the pennate diatoms, which took only ~3.4 days to reach the maximum quantum yield, whereas the centric diatom Asteromphalus sp. reached maximum ΔF/F′m after ~10.4 days. The north patch measurements showed a different response; the smallest cells never reached maximum ΔF/F′m, whereas the size fraction containing the largest cells did. Single‐celled measurements made nearly 30 days after the initial iron enrichment suggested that diatoms were experiencing either silicic acid or iron limitation, whereas measurements of Phaeocystis sp. did not. These data represent the first study of in situ recovery rates of PSII for groups of diatoms, and may help elucidate the mechanisms of species change in response to environmental perturbation. 相似文献
17.
Leaf Gas Exchange and Chlorophyll Fluorescence Parameters in Phaseolus Vulgaris as Affected by Nitrogen and Phosphorus Deficiency 总被引:2,自引:0,他引:2
The effects of N and P deficiency, isolated or in combination, on leaf gas exchange and fast chlorophyll (Chl) fluorescence
emission were studied in common bean cv. Negrito. 10-d-old plants grown in aerated nutrient solution were supplied with high
N (HN, 7.5 mol m−3) or low N (LN, 0.5 mol m−3), and also with high P (HP, 0.5 mol m−3) or low P (LP, 0.005 mol m−3). Regardless of the external P supply, in LN plants the initial fluorescence (F0) increased 12 % in parallel to a quenching of about 14 % in maximum fluorescence (Fm). As a consequence, the variable to maximum fluorescence ratio (Fv/Fm) decreased by about 7 %, and the variable to initial fluorescence ratio (Fv/F0) was lowered by 25 % in relation to control plants. In LP plants, Fv/Fm remained unchanged whilst Fv/F0 decreased slightly as a result of 5 % decline in Fm. Under N deficiency, the net photosynthetic rate (P
N) halved at 6 d after imposition of treatment and so remained afterwards. As compared to LN plants, P
N declined in LP plants latter and to a less extent. From 12 d of P deprivation onwards. P
N fell down progressively to display rates similar to those of LN plants only at the end of the experiment. The greater P
N in LP plants was not reflected in larger biomass accumulation in relation to LN beans. In general, P and N limitation affected
photosynthesis parameters and growth without showing any synergistic or additive effect between deficiency of both nutrients.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
18.
Abies faxoniana is a key species in reforestation processes in the southeast of the Qinghai-Tibetan Plateau of China. The changes in growth,
photosynthesis and nutrient status of A. faxoniana seedlings exposed to enhanced ultraviolet-B (UV-B), nitrogen supply and their combination were investigated. The experimental
design included two levels of UV-B treatments (ambient UV-B, 11.02 KJ m−2 day−1; enhanced UV-B, 14.33 KJ m−2 day−1) and two nitrogen levels (0; 20 g N m−2). The results indicated that: (1) enhanced UV-B significantly caused a marked decline in growth parameters, net photosynthetic
rate (Pn), photosynthetic pigments and F
v/F
m, (2) supplemental nitrogen supply increased the accumulation of total biomass, Pn, photosynthetic pigments and F
v/F
m under ambient UV-B, whereas supplemental nitrogen supply reduced Pn, and not affect biomass under enhanced UV-B, (3) enhanced
UV-B or nitrogen supply changed the concentration of nutrient elements of various organs. 相似文献
19.
Contrasting traits,contrasting environments,and considerations on population dynamics under a changing climate: an ecophysiological field study of two co‐dominant tree species
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Edgard A. Bontempo e Silva Kiyomi Ono Akihiro Sumida Shigeru Uemura Toshihiko Hara 《Plant Species Biology》2016,31(1):38-49
The increase in light availability resulting from canopy changes or opening is not always beneficial and can inhibit photosynthesis of tree seedlings already under other environmental stress. Tree seedlings' responses to compounded abiotic stress depend on their life‐history traits, and understanding the variations of such responses is important for understanding population dynamics under a changing climate. In this study we investigate how the photosynthesis of juveniles of two canopy tree species with different life‐history traits, Abies sachalinensis and Betula ermanii, differs in two contrasting sites at a sub‐boreal forest in northern Japan—one under a deciduous canopy (Closed site) and the other at a wide canopy opening (Open site). Seedlings at the Open site had low Fv/Fm (quantum yield of photosystem II) for a longer period than those at the Closed site. Abies sachalinensis at the Closed site showed lower Fv/Fm in spring than those at the Open site, but recovered after the canopy's new leaves flushed, indicating its acclimation to the shaded condition. Mean Pmax (light‐saturated photosynthetic rate at ambient CO2 levels) of A. sachalinensis seedlings was affected by site and air temperature, while B. ermanii seedlings were also affected by precipitation. Only B. ermanii's seedlings presented growth in the period studied, in spite of observed mid‐day drops to Fv/Fm attributed to water‐deficit‐related photoprotection. Results suggest that the climate change predicted for the Hokkaido area may increase the competitive advantage of broad‐leaved deciduous species, such as B. ermanii, in relation to evergreen conifers like A. sachalinensis. 相似文献
20.
The PSII photochemical activity in a terrestrial cyanobacterium Nostoc commune Vaucher ex Bornet et Flahault during rewetting was undetectable in the dark but was immediately recognized in the light. The maximum quantum yield of PSII (Fv/Fm) during rewetting in the light rose to 85% of the maximum within ~30 min and slowly reached the maximum within 6 h, while with rewetting in the darkness for 6 h and then exposure to light the recovery of Fv/Fm required only ~3 min. These results suggested that recovery of photochemical activity might depend on two processes, light dependence and light independence, and the activation of photosynthetic recovery in the initial phase was severely light dependent. The inhibitor experiments showed that the recovery of Fv/Fm was not affected by chloramphenicol (CMP), but severely inhibited by 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea (DCMU) in the light, suggesting that the light‐dependent recovery of photochemical activity did not require de novo protein synthesis but required activation of PSII associated with electron flow to plastoquinone. Furthermore, the test indicated that the lower light intensity and the red light were of benefit to its activation of photochemical activity. In an outdoor experiment of diurnal changes of photochemical activity, our results showed that PSII photochemical activity was sensitive to light fluctuation, and the nonphotochemical quenching (NPQ) was rapidly enhanced at noon. Furthermore, the test suggested that the repair of PSII by de novo protein synthesis played an important role in the acclimation of photosynthetic apparatus to high light, and the heavily cloudy day was more beneficial for maintaining high photochemical activity. 相似文献